Electrochemical Analysis of Single Glucose Oxidase with a Nanopipette

作     者：WANG Yongyong MA Hui LONG Yi-Tao YING Yi-Lun 

作者机构：School of Chemistry and Chemical EngineeringMolecular Sensing and Imaging Center(MSIC)Nanjing UniversityNanjing210023P.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2024年第40卷第2期

页      面：272-278页

核心收录：

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.22104052 2233000271 22027806) 

主　　题：Nanopore Single-molecule analysis Glucose oxidase Electrophoretic force Electroosmotic flow 

摘      要：Nanopore-based electrochemical technique is a promising tool for detecting single ***,detecting single proteins using a nanopipette in their native state without labeling is challenging due to the rapid translocation,which results in an inefficient signal *** our study,we finely tuned the driving force equilibrium between electrophoretic force(EPF)and electroosmotic flow(EOF)inside the nanopipette for efficient sensing of single glucose oxidase(GOD)*** duration time of GOD within the nanopipette is extended to about 4 *** strategy provided clear ionic current signals with a signal-to-noise ratio of *** EPF increased in the direction opposite to the motion of GOD,we observed a nonlinear growth in GOD’s duration *** extended the duration to about 4.4 times longer at−1000 mV compared to at−800 ***,nanopore-based electrochemical sensing could be used for single GOD molecule analysis as an ultrasensitive method.