Electrochemical Analysis of Single Glucose Oxidase with a Nanopipette
作者机构：School of Chemistry and Chemical EngineeringMolecular Sensing and Imaging Center(MSIC)Nanjing UniversityNanjing210023P.R.China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2024年第40卷第2期
页 面：272-278页
核心收录：
学科分类：07[理学] 0703[理学-化学] 0701[理学-数学] 070101[理学-基础数学]
基 金：supported by the National Natural Science Foundation of China(Nos.22104052 2233000271 22027806)
主 题：Nanopore Single-molecule analysis Glucose oxidase Electrophoretic force Electroosmotic flow
摘 要：Nanopore-based electrochemical technique is a promising tool for detecting single ***,detecting single proteins using a nanopipette in their native state without labeling is challenging due to the rapid translocation,which results in an inefficient signal *** our study,we finely tuned the driving force equilibrium between electrophoretic force(EPF)and electroosmotic flow(EOF)inside the nanopipette for efficient sensing of single glucose oxidase(GOD)*** duration time of GOD within the nanopipette is extended to about 4 *** strategy provided clear ionic current signals with a signal-to-noise ratio of *** EPF increased in the direction opposite to the motion of GOD,we observed a nonlinear growth in GOD’s duration *** extended the duration to about 4.4 times longer at−1000 mV compared to at−800 ***,nanopore-based electrochemical sensing could be used for single GOD molecule analysis as an ultrasensitive method.
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