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Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

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Stem Cell Discovery 2014年第3期4卷 61-66页

作者： Rosa maría López-Franco Jorge E. Moreno-Cuevas maría teresa gonzález-garza Servicio de Terapia Celular School of Medicine CITES Tecnológico de Monterrey Monterrey México

Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.

关键词： Stem Cells Recovery CD133+ Percoll Density Gradients

Lovastatin as a supplement to mitigate rumen methanogenesis:an overview

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Journal of Animal Science and Biotechnology 2022年第2期13卷 353-364页

作者： Amaury Ábrego-Gacía Héctor M.Poggi-Varaldo Vania Robles-gonzález teresa Ponce-Noyola Graciano Calva-Calva Elvira Ríos-Leal Daniel Estrada-Bárcenas Alfredo Mendoza-Vargas Department of Biotechnology and Bioengineering CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Environmental Biotechnology and Renewable Energies Group CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Instituto de Hidrología Universidad Tecnológica de la MixtecaOaxaca 69000 Huajuapan de LeónMexico National Collection of Microbial and Cellular Cultures CINVESTAV-IPNP.O.Box17-74007000 Mexico CityMexico Unidad de Secuenciación e Identificación de Polimorfismos Instituto Nacional de Medicina Genómica14610 Mexico CityMexico

Methane from enteric fermentation is the gas with the greatest environmental impact emitted by ***(Lv)addition to feedstocks could be a strategy to mitigate rumen methane emissions via decreasing the population of methanogenic archaea(ma).Thus,this paper provides the first overview of the effects of Lv supplementation,focusing on the inhibition of methane production,rumen microbiota,and ruminal *** indicated that Lv treatment had a strong anti-methanogenic effect on pure strains of ***,there are uncertainties from in vitro rumen fermentation trials with complex substrates and rumen ***-state fermentation(SSF)has emerged as a cost-effective option to produce *** this way,SSF of agricultural residues as an Lv-carrier supplement in sheep and goats demonstrated a consistent decrease in ruminal methane *** experimental evidence for in vitro conditions showed that Lv did not affect the volatile fatty acids(VFA).However,in vivo experiments demonstrated that the production of VFA was *** did not negatively affect the digestibility of dry matter during in vitro and in vivo methods,and there is even evidence that it can induce an increase in *** the rumen microbiota,populations of ma were reduced,and no differences were detected in alpha and beta diversity associated with Lv ***,some changes in the relative abundance of the microbiota were *** studies are recommended on:(i)Lv biodegradation products and stability,as well as its adsorption onto the solid matter in the rumen,to gain more insight on the“available”or effective Lv concentration;and(ii)to determine whether the effect of Lv on ruminal fermentation also depends on the feed composition and different ruminants.

关键词： Fermentation Lovastatin Methanogenesis Microbiota Rumen

Antiprotozoal Activity of a <i>Thymus vulgaris</i>Methanol Extract and Its Fractions

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Health 2017年第7期9卷 1081-1094页

作者： Jesús Norberto garza-gonzález Javier Vargas-Villarreal ma Julia Verde-Star Catalina Rivas-Morales Azucena Oranday-Cárdenas magda E. Hernandez-García Laura De La garza-Salinas Francisco gonzález-Salazar Department of Chemistry Phytochemistry and Analytical Chemistry Lab Faculty of Biological Sciences University Autonomous of Nuevo Leon San Nicolas de los Garza Mexico Department of Basic Sciences Division of Health Sciences University of Monterrey San Pedro Garza Garcia Mexico Laboratory of Biochemistry and Cell Physiology Division of Cellular and Molecular Biology Center for Biomedical Research of the Northeast Mexican Social Security Institute Monterrey Mexico Mexican Social Security Institute Nuevo Leon Delegation Monterrey Mexico

Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.

关键词： Thymus vulgaris Trichomonas vaginalis Giardia lamblia Entamoeba histolytica Antiprotozoal Agents

Emerging Water Quality Issues along Rio de la Sabana, Mexico

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Journal of Water Resource and Protection 2018年第7期10卷 621-636页

作者： Daniel Pineda-Mora Jeiry Toribio-Jiménez teresa Leal-Ascencio ma Ana Laura Juárez-López Justiniano gonzález-gonzález Jesús Carlos Ruvalcaba-Ledezma Ramón Alberto Batista-García Paul Alexander Arp Local Development Science Center Autonomous University of Guerrero Acapulco Mexico Faculty of Chemistry and Biological Science Autonomous University of Guerrero Chilpancingo Mexico Faculty of Chemistry Science University of Veracruz Xalapa Mexico Health Science Institute Autonomous University of Hidalgo Pachuca Mexico Cell Dynamics Research Center Autonomous University of Morelos Cuernavaca Mexico Faculty of Forestry and Environmental management University of New Brunswick Fredericton Canada

The basin of Rio de la Sabana is the largest tributary of the Tres Palos coastal lagoon in Southwest Mexico, east of Acapulco. This lagoon and its upstream basin areas have become a high priority area for the preservation of coastal and marine environments. To obtain information about water quality as affected by urban expansion since 2002, fourteen physicochemical parameters (temperature, pH, electrical conductivity, dissolved oxygen, ammonium, nitrate, nitrite, sulphate, phosphate), biochemical (biological and chemical oxygen demand, methylene blue active substances) and bacteriological parameters (total and fecal coliforms) were determined. This sampling was done for dry and rainy season conditions at seven locations (S1, S2, S3, …, S7) along the river, spaced 3 to 6 km apart to a total of 30.4 km. The results were grouped into four zones: (Z1) reference, (Z2) transition, (Z3) polluted, (Z4) recovery. The Alborada (S5) and Tunzingo (S6) sites, adjacent to dense high-class residential areas (Z3), had the greatest pollution charges in both seasons, while the La Poza (S7) site near the Tres Palos lagoon (Z4) showed a decrease in pollution. All parameters correlated with increasing head- to down-river sampling distance by following linear (pH, DO) or curvilinear patterns (all other parameters). Using sampling location and dry versus rainy sampling season as multivariate regression (predictor) variables led to least-squares capturing: 1) 66% to 95% of the T(°C), pH, DO, and PO3-4 variations, and 2) 57% to 96% of the log-linear variations of the other parameters. Among the parameters, T(°C), DO, and PO3-4 were not significantly affected by sampling season, while pH became so after deleting two higher than usual pH values at the S5 and S6 locations during the dry season.

关键词： Rio de la Sabana Urban Developments Water Pollution Sampling Locations Dry/Rainy Season Multivariate Analyses

Evaluation of the Hydroxynitrile Lyase Activity in Cell Cultures of Capulin (Prunus serotina)

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生物工程学报 2008年第7期24卷 1199-1201页

作者： Liliana Hemandez Hector Luna Arturo Navarro-Ocafia ma teresa de Jesus Olivera-Flores Ivon Ayala UniversidadAutdnoma Metropolitana UnidadXochimilco Depto. Sistemas Biologicos A. P. 23/181 Mgxico D. F. Mexico Facultad de Qufmica Universidad Nacional Autdnoma de Mexico Ciudad Universitaria Mgxico D. F. 04510 Mexico

Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The threeweek old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.

关键词：细胞裂解酶活性柳叶野黑桃

Specific metabolic biomarkers as risk and prognostic factors in colorectal cancer

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World Journal of Gastroenterology 2014年第29期20卷 9759-9774页

作者： magorzata Muc-Wierzgoń Ewa Nowakowska-Zajdel Sylwia Dziegielewska-Gesiak teresa Kokot Katarzyna Klakla Edyta Fatyga Elzbieta Grochowska-Niedworok Dariusz Waniczek Janusz Wierzgoń Department of Internal Diseases Medical University of Silesia Department of Human Nutrition Medical University of Silesia Department of Gastrointestinal Surgery Medical University of Silesia Department of Oncological and Reconstructive Surgery Center of Oncology Maria Skodowska-Curie Memorial Institute in Warsaw

Advances in genomics,molecular pathology and metabolism have generated many candidate biomarkers of colorectal cancer with potential clinical *** and biological studies suggest a role for adiposity,dyslipidaemia,hyperinsulinemia,altered glucose homeostasis,and elevated expression of insulin-like growth factor(IGF)axis members in the risk and prognosis of *** review discusses some recent past and current approaches being taken by researches in obesity and metabolic *** authors describe three main systems as the most studied metabolic candidates of carcinogenesis:dyslipidemias,adipokines and insulin/IGF ***,each of these components is unsuccessful in defining the diseases risk and progression,while their co-occurrence increases cancer incidence and mortality in both men and women.

关键词： Colorectal cancer Metabolic biomarkers Risk Prognosis Dyslipidemias Adipokines Insulin-like growth factor-system

Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy

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Stem Cell Discovery 2014年第1期4卷 1-7页

作者： maria teresa gonzalez-garza Citlali Alcaraz Lizbeth gonzalez-Jara Demetrio Arcos Jorge E. Moreno Cuevas Servicio de Terapia Celular School of Medicine Centro de Innovación y Transferencia en Salud Tecnológico de Monterrey Monterrey México

Stem cell therapy for cardiac infarct regeneration has been widely used in clinical research. Despite the fact that important advances in this field have been reached, the observed recovery does not demonstrate new cardiac muscle formation. Benefits have been observed due to an improvement in neovascularisation. The main objective of this study was to determine if pre-differentiated stem cells into cells of myocardic lineage are capable of engraftment in animal models with induced cardiac infarct and are capable of truly differentiating into myocardiocytes. Bone marrow rat stem cells were pre-differentiated with 5-AZ. After 4 weeks, pre-differentiated stem cells express muscarinic 1, 2 and β adrenergic 2 receptors. Also, proteins such as sarcomeric α-actin, cardiac myosin heavy chain, desmin and vimentin were detected by immunocytochemistry. Cells were transplanted intracardialy in an ischemic cardiac rat model. Pre-differentiated or non differentiated cells were transplanted after 4 weeks post infarct induction. Histopathology of the hearts was made 2 weeks after cell transplantation. Typical granulated tissue, scare formation and neovascularisation were observed in both groups. However, in those hearts from rats inoculated with pre-differentiated cells many appeared atypical and were α-actin sarcomeric positive. These events suggest that pre-differentiated cells conserve some muscle characteristic traits in situ that at least last for two weeks after transplantation.

关键词： Cell Therapy MSC Regenerative Medicine Cardiocyte Differentiation Stem Cells

Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data

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Journal of Integrative Agriculture 2018年第11期17卷 2509-2517页

作者： Daniel Bravo-Perez ma. teresa Santillbn-Galicia Roberto M. Johansen-Naime Hector gonzalez-Hernandez Obdulia L. Segura-Leon Daniel L, Ochoa-martinez Stephanie Guzman-Valencia Posgrado en Fitosanidad-Entomologia y Acarologia Colegio de Postgraduados Km 36.5 Carretera Mexico-Texcoco Montecillo Texcoco Estado de Mexico 56230 Mexico Instituto de Biologia de la Universidad Nacional Autonoma de Mexico (IBUNAM) Circuito exterior s/n Ciudad Universitaria Coyoacan Ciudad de Mexico 04510 Mexico Posgrado en Fitosanidad-Fitopatologia Colegio de Postgraduados Km 36.5 Carretera Mexico-Texcoco Montecillo Texcoco Estado de Mexico 56230 Mexico

Avocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal reports on species diversity of thrips in Mexico are very scarce. Morphological identification can sometimes be time-consuming and inconclusive. Therefore, we explored the species diversity of thrips in Mexican avocado orchards （Michoacan state） based on partial sequences of the mitochondrial gene cytochrome oxidase subunit I （COl）. Forty-four specimens were analysed, which represented approximately 8% of all individuals collected from five localities distributed in three Municipalities. All specimens were analysed using the COl marker, and specimens within the genera Frankliniella were also analysed using a marker within the D2 domain of the 28S （28SD2） nuclear ribosomal DNA. Molecular identifications were confirmed using morphological taxonomy. Overall, six genera were found （Neohydatothrips, Scirtothrips, Frankliniella, Arorathrips, Caliothrips and Leptothrips）. AII genera contained only one species, except Frankliniella, for which there were six species. Data from the two molecular markers suggest the existence of cryptic species within Mexican F. occidentalis populations.

关键词： molecular taxonomy morphological identification cytochrome oxidase subunit I 28S nuclear ribosomal DNA.

Synchronous and metachronous neoplasms in gastric cancer patients:A 23-year study

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World Journal of Gastroenterology 2014年第23期20卷 7480-7487页

作者： magorzata Lawniczak Alicja Gawin Halina Jaroszewicz-Heigelmann Wiesawa Rogoza-mateja Joanna Raszeja-Wyszomirska Andrzej Biaek Katarzyna Karpińska-Kaczmarczyk teresa Starzyńska Department of Gastroenterology Pomeranian Medical University71-252 SzczecinPoland Liver Unit Pomeranian Medical University70-111 SzczecinPoland Department of Pathology Pomeranian Medical UniversityUnii Lubelskiej 171-252 SzczecinPoland

AIM: To determine the prevalence and characteristics of additional primary malignancies in gastric cancer (GC) patients.

关键词： Gastric cancer Multiple primary cancers Synchronous Metachronous Blood groups