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云南白菜根肿病拮抗菌的筛选及鉴定

西南农业学报 2024年第1期37卷 137-144页

作者：张怡宁刘秋艳朱美竹曾民苏源梁家灿刘庆姚永兴周晓罡昆明学院农学与生命科学学院昆明650214 云南省农业科学院生物技术与种质资源研究所昆明650223 云南省农业生物技术重点实验室昆明650223 农业部西南作物基因资源与种质创制重点实验室昆明650223 云南昊盟农业科技有限公司云南楚雄651604

【目的】获得对云南白菜根肿病具有高效拮抗作用的微生物菌株,为云南白菜根肿病的生物防治提供潜在的菌种资源。【方法】从云南省曲靖市麒麟区三宝乡大棚白菜根肿病发病严重的地块,采集正常生长或发病较轻的白菜植株及其根际土壤,分离... 详细信息

【目的】获得对云南白菜根肿病具有高效拮抗作用的微生物菌株,为云南白菜根肿病的生物防治提供潜在的菌种资源。【方法】从云南省曲靖市麒麟区三宝乡大棚白菜根肿病发病严重的地块,采集正常生长或发病较轻的白菜植株及其根际土壤,分离筛选抑菌效果较好的菌株,并对其进行形态特征鉴定、生理生化检测及分子序列分析。【结果】经初步筛选共获得46株细菌。土壤样品中共分离出30株细菌,其中抑制效果最好的是TB⁃7菌株,该菌株对白菜根肿病菌休眠孢子萌发抑制率为76.88%;白菜植株内共分离出16株内生细菌,其中抑制效果最好的是EB⁃15菌株,该菌株对白菜根肿病菌休眠孢子萌发抑制率为67.38%。通过对比分析选择抑菌效果最好的TB⁃7菌株作为目标菌株,经鉴定,TB⁃7菌株为革兰氏阳性芽孢杆菌,菌体菌落较小,颜色淡黄色,表面光滑且扁平,菌体湿润,不透明,边缘规则;生理生化指标检测结果显示,TB⁃7菌株是好氧菌,V⁃P、淀粉水解、pH 5.7,生长3项检测指标均呈阳性,柠檬酸盐、丙酸盐、D⁃木糖、L⁃阿拉伯糖、D⁃甘露醇、明胶液化、7%NaCl生长、硝酸盐还原8项检测指标均呈阴性;16S rDNA扩增产物约为1500 bp,测序结果表明,TB⁃7菌株与坚强芽孢杆菌(Cytobacillus firmus)同源性达到100%,鉴定该菌株为芽孢杆菌属的坚强芽孢杆菌。【结论】通过初步筛选及鉴定,获得1株对白菜根肿病菌休眠孢子抑制率达到76.88%的拮抗菌株,该菌株的获得可为解决云南本地日益严重的白菜根肿病的生物防治奠定基础。

关键词：根肿病生防菌休眠孢子菌种鉴定

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Hydrothermal Synthesis of Polyoxovanadate-based MOFs Microsphere as Supercapacitor

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Chinese Journal of Structural Chemistry 2019年第4期38卷 581-586页

作者： zhu mei-Hua WU Yi-Wei GUO Hong-Xu College of Chemistry and Environment Minnan Normal University

Polyoxovanadate-based MOFs microsphere electrode material, [Ni_2(4,4?-bipyridine)_3(H_2O)_2V_4O_(12)]·2.5 H_2O, was synthesized through a simple hydrothermal method, and then characterized through FT-IR, XRD and SEM. Electrochemical properties of the sample as supercapacitor electrodes in aqueous electrolyte were developed and studied by cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy techniques. Results showed that a high specific capacitance of 152.36 F g^(-1) at current density of 1 A g^(-1) in 2 M KOH electrolyte, and 97.62% retention after 2500 charge-discharge cycles at current density of 4 A g^(-1), which is promising for applications in supercapacitors.

关键词： polyoxovanadate microsphere supercapacitor electrode material

A General Strategy for Efficiently Constructing Multifunctional Cluster Fillers Using a Three-Fluid Nozzle Spray Drying Technique for Dental Restoration

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Engineering 2022年第1期8卷 138-147页

作者： Dan-Lei Yang Dan Wang Hao Niu Rui-Li Wang mei Liu Fei-Min Zhang Jie-Xin Wang mei-Fang zhu State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Research Center of the Ministry of Education for High Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Jiangsu Key Laboratory of Oral Diseases Department of ProsthodonticsAffiliated Hospital of StomatologyNanjing Medical UniversityNanjing 210029China

Multifunctional fillers are greatly required for dental resin composites(DRCs).In this work,a spray dryer with a three-fluid nozzle was applied for the first time to construct high-performance complex nanoparticle clusters(CNCs)consisting of different functional nanofillers for dental *** application of a three-fluid nozzle can effectively avoid the aggregation of different nanoparticles with opposite zeta potentials before the spray drying process in order to construct regularly shaped *** a SiO_(2)–ZrO_(2) binary system,the SiO_(2)–ZrO_(2) CNCs constructed using a three-fluid nozzle maintained their excellent mechanical properties((133.3±4.7)MPa,(8.8±0.5)GPa,(371.1±13.3)MPa,and(64.5±0.7)HV for flexural strength,flexural modulus,compressive strength,and hardness of DRCs,respectively),despite the introduction of ZrO_(2) nanoparticles,whereas their counterparts constructed using a two-fluid nozzle showed significantly decreased mechanical ***,heat treatment of the SiO_(2)-ZrO_(2) CNCs significantly improved the mechanical properties and radiopacity of the *** DRCs containing over 10%mass fraction ZrO_(2) nanoparticles can meet the requirement for radiopaque *** importantly,this method can be expanded to ternary or quaternary *** filled with SiO_(2)-ZrO_(2)-ZnO CNCs with a ratio of 56:10:4 displayed high antibacterial activity(antibacterial ratio>99%)in addition to excellent mechanical properties and ***,the three-fluid nozzle spray drying technique holds great potential for the efficient construction of multifunctional cluster fillers for DRCs.

关键词： Multifunctional cluster fillers Three-fluid nozzle spray drying Mechanical properties Antibacterial activity Radiopacity Dental resin composites

A novel terbium complex using oxadiazole derivative as a neutral ligand:Synthesis and properties

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Chinese Chemical Letters 2007年第5期18卷 573-576页

作者： Yu Liu Kong Qiang Xing Ji Yong Deng mei Xiang zhu Xia Yu Wang Wei Guo zhu College of Chemistry Xiangtan University Xiangtan 411105 China Department of Chemistry Qiongzhou University Wuzhishan 572200 China

A novel terbium complex using 1,3,4-oxadiazole derivative as a neutral ligand was synthesized and characterized. Its thermal stability and photoluminescent properties were studied. The strong emission peaked at 546 nm with a full width at half maximum of 5 nm was observed in the pure terbium complex film under excitation of 328 nm light, which is attributed to the characteristic emission of terbium ion. The good thermal stability and intense sharp emission of this terbium complex display its potential application in electroluminescence devices.

关键词： rerbium complex 1,3,4-Oxadiazole Synthesis Photoluminescence

Attitude maneuver of liquid-filled spacecraft with a flexible appendage by momentum wheel

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Acta Mechanica Sinica 2012年第2期28卷 543-550页

作者： Dan-Dan Yang Bao-Zeng Yue Wen-Jun Wu Xiao-Juan Song Le-mei zhu Department of Mechanics School of Aerospace EngineeringBeijing Institute of Technology100081 BeijingChina

Attitude maneuver of liquid-filled spacecraft with an appendage as a cantilever beam by momentum wheel is studied. The dynamic equations are derived by conserva- tion of angular momentum and force equilibrium principle. A feedback control strategy of the momentum wheel is ap- plied for the attitude maneuver. The residual nutation of the spacecraft in maneuver process changes with some chosen parameters, such as steady state time, locations of the liq- uid container and the appendage, and appendage parame- ters. The results indicate that locations in the second and fourth quadrants of the body-fixed coordinate system and the second quadrant of the wall of the main body are better choices *** the liquid containers and the appendage than other locations if they can be placed randomly. Higher density and thicker cross section are better for lowering the residual nutation if they can be changed. Light appendage can be modeled as a rigid body, which results in a larger residual nutation than a flexible model though. The resid- ual nutation decreases with increasing absolute value of the initial sloshing angular height.

关键词： Liquid-filled spacecraft Appendage Attitude maneuver Momentum wheel Steady state time Residual nutation

早期序贯康复护理联合童趣化互动干预对外伤性颅内出血患儿的影响

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护理实践与研究 2024年第6期21卷 821-827页

作者：黄霞张莉狄红珍冯小丹朱梅苏州大学附属儿童医院神经外科江苏省苏州市215025

目的探究早期序贯康复护理联合童趣化互动干预对外伤性颅内出血患儿的影响。方法选取2021年12月—2022年11月在医院进行外伤性颅内出血治疗的100例患儿为研究对象,按照组间基本特征具有可比性的原则将其分为对照组与观察组,每组50例... 详细信息

目的探究早期序贯康复护理联合童趣化互动干预对外伤性颅内出血患儿的影响。方法选取2021年12月—2022年11月在医院进行外伤性颅内出血治疗的100例患儿为研究对象,按照组间基本特征具有可比性的原则将其分为对照组与观察组,每组50例。对照组开展常规护理,观察组开展早期序贯式康复护理联合童趣化互动干预。比较两组患儿干预前后神经功能、营养状态、运动功能及生存质量。结果两组患儿实施干预方法前上肢、下肢的肢体运动功能评分比较差异均无统计学意义(P>0.05);观察组患儿实施早期序贯康复护理联合童趣化互动干预后上肢、下肢运动评分高于对照组干预后,差异有统计学意义(P0.05);观察组患儿实施早期序贯康复护理联合童趣化互动干预后NIHSS评分低于对照组干预后,组间比较差异有统计学意义(P0.05);观察组患儿实施早期序贯康复护理联合童趣化互动干预后RBP及PAB高于对照组干预后,差异有统计学意义(P0.05);观察组患儿实施早期序贯康复护理联合童趣化互动干预后各维度生存质量评分高于对照组干预后,差异有统计学意义(P<0.05)。结论对外伤性颅内出血患儿实施早期序贯式康复护理联合童趣化互动干预,能够有效改善患儿神经功能,同时有助于提高患儿运动功能及生存质量。

关键词：早期序贯式康复护理童趣化互动干预外伤性颅内出血神经功能运动功能生活质量

Blood pressure circadian rhythm and heart rate turbulence in hypertensive patients: relationship with left ventricular hypertrophy

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Journal of Geriatric Cardiology 2009年第1期6卷 38-41页

作者： mei zhu Mohan Liu Xinhong Guo Shiwen Wang Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China

Objective To investigate the relationship of blood pressure circadian rhythm with myocardial hypertrophy and the changes of autonomic nerve function in patients with essential hypertension (EH). Methods Eighty-two female patients with essential hypertension (EH) underwent 24-hours ambulatory blood pressure monitorings (ABPM), dynamic electrocardiogram (Holter) and echocardiography examination. Patients were classified into non-dipping group (n=40) and dipping group (n=42) according to the result of ABPM. Left ventricular mass index (LVMI), heart rate variability (HRV) in time domain (including SDNN, SDANN, rMSSD, PNN50) and heart rate turbulence (HRT) parameters (including turbulence onset [TO] and turbulence slope [TS]) were measured. Results Compared with those in dipping group, patients in non-dipping group have higher incidence of LVH (19.0% vs 52.5%, P<0.01), greater mean LVMI (112.39±12.79 g/m2 vs 121.98±13.35 g/m2, P<0.01), decreased PNN50 and rMSSD. TS value was decreased while TO was increased in non-dipping group compared with those in dipping group (both P <0.01); patients with LVH showed decreased TS and increased TO, compared with those without LVH. Conclusion In female patients with EH, non-dipping blood pressure circadian is associated with higher incidence of LVH. The HRV and HRT were more remarkably blunted in non-dipping patients, as well as those with LVH.

关键词： Hypertension myocardial hypertrophy heart rate variability heart rate turbulence

Unusual magnetic resonance imaging findings of brain and leptomeningeal metastasis in lung adenocarcinoma:A case report

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World Journal of Clinical Cases 2022年第5期10卷 1723-1728页

作者： Na Li Yu-Jun Wang Fang-mei zhu Shui-Tang Deng Department of Radiology Tongde Hospital of Zhejiang ProvinceHangzhou 310012Zhejiang ProvinceChina Department of Radiology Zhejiang Provincial Hospital of Chinese MedicineHangzhou 310012Zhejiang ProvinceChina

BACKGROUND Metastatic tumors are the most common malignancies of central nervous system in adults,and the frequent primary lesion is lung *** and leptomeningeal metastases are more common in patients with non-small-cell lung cancer harboring epidermal growth factor receptor ***,the coexist of brain metastasis with leptomeningeal metastasis(LM)in isolated gyriform appearance is *** SUMMARY We herein presented a case of a 76-year-old male with an established diagnosis as lung adenocarcinoma with gyriform-appeared cerebral parenchymal and leptomeningeal metastases,accompanied by mild peripheral edema and avid contrast enhancement on magnetic resonance *** and pathological examinations confirmed the brain and leptomeningeal metastatic lesions in the left frontal cortex,subcortical white matter and local *** This case was unique with respect to the imaging findings of focal gyriform appearance,which might be caused by secondary parenchymal brain metastatic tumors invading into the leptomeninges or coexistence with *** should be aware of this uncommon imaging presentation of tumor metastases to the central nervous system.

关键词： Brain metastasis Leptomeningeal metastasis Magnetic resonance imaging Lung cancer Epidermal growth factor receptor Case report

Preparation and purification of F(ab′) _2 fragment from anti hepatoma mouse IgG_1 mAb

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World Journal of Gastroenterology 1999年第6期5卷 522-524页

作者： LIU Cheng Gang 1, zhu mei Cai 1 and CHEN Zhi Nan 2 Center of Clinical Molecular Biology General Hospital of Air Force Beijing 100036 China Center of Clinical Molecular Biology General Hospital of Air Force Fu Cheng Road 30 Beijing 100036 China Department of Pathology Fourth Military Medical University Xi'an 710032 Shaanxi Province China

INTRODUCTIONSince the advent of hybridoma technology[1], monoclonal antibodies have been widely used in basic studies and clinical application. F(ab')2 is a bivalent antibody fragment which is currently used for both diagnosis and treatment[2], and better than the original mAbs, because it does not retain complement-binding function due to lack of Fc regions and reduced interaction with non-specific proteins and the smaller molecular weight than the mAbs, furthermore, it can be digested by pepsin or papain and purified by size exclusion chromatography[4], ion-exchange chromatography[6] or hydrophobic interaction[7]. However, these methods are time-consuming, and cannot attain sufficient purity and recovery of F(ab')2 fragment. Development of efficient procedures for F(ab')2 preparation is an urgent necessity.

关键词： antibody, monoclonal F(ab′) 2 fragment papain digestion liver neoplasms ion exchange chromatography