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Photosynthetic and Molecular Markers of CO_2-mediated Photosynthetic Downregulation in Nodulated Alfalfa

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Journal of Integrative Plant Biology 2013年第8期55卷 721-734页

作者： Alvaro Sanz-Sez Gorka Erice Iker Aranjuelo Ricardo Aroca Juan manuel Ruíz-Lozano Jone Aguirreolea Juan Jos Irigoyen manuel sanchez-diaz Department of Plant Biology University of NavarraPamplonaE-31008Spain Department of Plant Biology University of BarcelonaBarcelonaE-8028Spain Department of Plant Biology University of Illinois at Urbana-Champaign61801USA Agrobiotechnology Institute Public University of NavarraMutilva BajaE-31192Spain Department of Soil Microbiology and Symbiotic Systems Zaidin Experimental Station(CSIC)GranadaE-18008.Spain

Elevated CO2 leads to a decrease in potential net photosynthesis in long-term experiments and thus to a reduction in potential growth. This process is known as photosynthetic downregulation. There is no agreement on the definition of which parameters are the most sensitive for detecting CO2 acclimation. In order to investigate the most sensitive photosynthetic and molecular markers of CO2 acclimation, the effects of elevated CO2, and associated elevated temperature were analyzed in alfalfa plants inoculated with different Sinorhizobium meliloti strains. Plants （Medicago sativa L. cv. Aragon） were grown in summer or autumn in temperature gradient greenhouses （TGG）. At the end of the experiment, all plants showed acclimation in both seasons, especially under elevated summer temperatures. This was probably due to the lower nitrogen （N） availability caused by decreased N2-fixation under higher temperatures. Photosynthesis measured at growth CO2 concentration, rubisco in vitro activity and maximum rate of carboxylation were the most sensitive parameters for detecting downregulation. Severe acclimation was also related with decreases in leaf nitrogen content associated with declines in rubisco content （large and small subunits） and activity that resulted in a drop in photosynthesis. Despite the sensitivity of rubisco content as a marker of acclimation, it was not coordinated with gene expression, possibly due to a lag between gene transcription and protein translation.

关键词： Carbon dioxide Medicago sativa (alfalfa) photosynthetic downregulation RbcL and RbcS Sinorhizobium meliloti.

Association between lifestyle factors and thyroid function in young euthyroid adults

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Food Science and Human Wellness 2024年第1期13卷 265-275页

作者： Elisa Merchan-Ramirez Guillermo sanchez-Delgado Lucas Jurado-Fasoli Francisco M Acosta manuel Munoz-Torres Jose M.Llamas-Elvira Jonatan R Ruiz Sport and Health University Research Institute(iMUDS) Faculty of Sports ScienceUniversity of GranadaGranada 18016Spain Pennington Biomedical Research Center Baton Rouge 70808USA Faculty of Medicine University of GranadaGranada 18071Spain Turku PET Centre University of TurkuTurku 20500Finland Turku PET Centre Turku University HospitalTurku 20520Finland Biosanitary Research Institute of Granada(Ibs.Granada) Granada 18012Spain CIBERFES Instituto de Salud CarlosⅢMadrid 28029Spain Endocrinology and Nutrition Service University Hospital San CecilioGranada 18016Spain Nuclear Medicine Service "Virgen de las Nieves"University HospitalGranada 18014Spain

Purpose:The present work examines the associations of dietary habits,sedentarism,physical activity(PA)levels and sleep habits,with thyroid function in young euthyroid ***:A total of 105 young euthyroid adults participated in this cross-sectional *** function was determined in fasting conditions(>6 h).Dietary habits were measured by a food frequency questionnaire and three non-consecutive 24 h recalls,and different dietary intake and patterns were then *** time spent in sedentary,PA levels and sleep habits were objectively measured using a wrist-worn ***:Energy and carbohydrate intake were positively associated with thyroid stimulating hormone(TSH)(β=0.222;R^(2)=0.102;P=0.022 andβ=0.425;R^(2)=0.129;P=0.007,respectively)whereas fat intake was negatively associated with TSH(β=-0.428;R^(2)=0.137;P=0.004).Energy intake was also positively associated with free triiodothyronine(β=0.277;R^(2)=0.137;P=0.004).Further,adherence to the Mediterranean diet was negatively related to TSH and free thyroxine(FT4)(β=-0.221;R^(2)=0.113;P=0.020 andβ=-0.268;R^(2)=0.071;P=0.007,respectively).Vigorous-intensity and overall PA were negatively associated with FT4(β=-0.227;R^(2)=0.052;P=0.022 andβ=-0.204;R^(2)=0.042;P=0.041,respectively).In contrast,no associations were found between sleep parameters and thyroid ***:Lifestyle factors such as dietary intake and PA levels seems to be related to thyroid function even in young euthyroid adults.

关键词： Thyroid hormones Dietary intake Sleep Physical activity Euthyroid

Clinicopathological alterations in wild mammals from the reservoir system of Trypanosoma cruzi:a scoping review

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Animal Diseases 2024年第1期4卷 48-58页

作者： Ricardo sanchez Pérez Josémanuel Aranda-Coello JoséAntonio De Fuentes Vicente Oscar Rico Chavez Facultad de Estudios Superiores Cuautitlan Universidad Nacional Autonoma deMexicoCuautitlan lzcalliEstadodeMexicoMexico El Colegio de la Frontera Sur-Unidad Campeche CampecheCampecheMexico Instituto de Ciencias Biologicas-Universidad de Ciencias y Artes de Chiapas Tuxtla GutierrezChiapasMexico Facultad de Medicina Veterinaria y Zootecnia-Universidad Nacional Autonoma deMexico Ciudad deMexicoMexico

Trypanosoma cruzi is the etiologic agent of Chagas *** flagellated protozoan is transmitted to humans as well as different species of domestic and wild animals via vectors from the Reduviidae family(known as"kissing bugs").Despite the fact that hundreds of species of wild mammals are part of the reservoir system,the morphologi-cal changes and clinical manifestations resulting from the pathogenesis of the infection have been largely *** aim of this review is to systematically compile the available information regarding clinicopathological altera-tions in wild mammals due to natural infection by *** was obtained from six online bibliographic data search platforms,resulting in the identification of 29 publications that met the inclusion *** was the most common clinical manifestation,cardiac damage was the main finding at necropsy,and lymphoplas-macytic inflammation was the most frequent microscopic ***,regardless of its role as a reservoir,*** has the potential to affect the health status of wild mammals,a situation that highlights the need for further research to analyze,measure,and compare its effects at both the individual and population levels.

关键词： Trypanosoma cruzi Chagas disease American trypanosomiasis Clinicopathological alterations Natural infection Wild mammals

Maintaining flexibility in smart grid consumption through deep learning and deep reinforcement learning

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Energy and AI 2023年第3期13卷 137-148页

作者： Fernando Gallego Cristian Martin manuel diaz Daniel Garrido ITIS Software Institute University of MalagaMalagaSpain

The smart grid concept is key to the energy revolution that has been taking place in recent *** Grids have been present in energy research since their ***,the scarcity of data from different energy sources,hardware power,or co-simulation environments has hindered their *** advances in multi-agent-based systems,the possibility of simulating the behavior of different energy sources,combining real building consumption,and simulated data,storage batteries and vehicle charging points,has opened *** development has resulted in much research published using both simulated and physical *** these investigations show that the main problem is that the machine learning algorithms do not fully match the real behavior,it is complex to use them to replicate the different actions to be *** paper aims to combine the approach of behavior prediction with state-of-the-art techniques,such as deep learning and deep reinforcement learning,to simulate unknown or critical system scenarios.A very important element in smart grids is the possibility of maintaining consumption within specific ranges(flexibility).For this purpose,we have made use of Tensorflow libraries that predict energy consumption and deep reinforcement learning to select the optimal actions to be performed in our *** developed platform is flexible enough to include new technologies such as smart batteries,electric vehicles,etc.,and it is oriented to real-time operation,being applied in an on-going real project such as the European ebalance-plus project.

关键词： Multi-agent based system Smart grid Distributed energy resources

Operational definitions and measurement of externalizing behavior problems:An integrative review including research models and clinical diagnostic systems

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World Journal of Psychiatry 2023年第6期13卷 278-297页

作者： Lidia Torres-Rosado Oscar M Lozano manuel sanchez-Garcia Fermín Fernández-Calderón Carmen diaz-Batanero Department of Clinical and Experimental Psychology University of HuelvaHuelva 21071Spain Research Center for Natural Resources Health and EnvironmentUniversity of HuelvaHuelva 21071Spain

Measurement of externalizing disorders such as antisocial disorders,attentiondeficit/hyperactivity disorder or borderline disorder have relevant implications for the daily lives of people with these *** the Diagnostic and Statistical Manual of Mental Disorders(DSM)and the International Classification of Diseases(ICD)have provided the diagnostic framework for decades,recent dimensional frameworks question the categorical approach of psychopathology,inherent in traditional *** and instruments develop under the DSM or ICD framework preferentially adopt this categorical approach,providing diagnostic *** contrast,dimensional measurement instruments provide an individualized profile for the domains that comprise the externalizing spectrum,but are less widely used in *** paper aims to review the operational definitions of externalizing disorders defined under these different frameworks,revise the different measurement alternatives existing,and provide an integrative operational ***,an analysis of the operational definition of externalizing disorders among the DSM/ICD diagnostic systems and the recent Hierarchical Taxonomy of Psychopathology(HiTOP)model is carried ***,in order to analyze the coverage of operational definitions found,a description of measurement instruments among each conceptualization is *** phases in the development of the ICD and DSM diagnosis systems can be observed with direct implications for *** and DSM versions have progressively introduced systematicity,providing more detailed descriptions of diagnostic criteria and categories that ease the measurement instrument ***,it is questioned whether the DSM/ICD systems adequately modelize externalizing disorders,and therefore their *** recent theoretical approaches,such as the HiTOP model seek to overcome some of the criticism raised towards the classification ***,several issues

关键词： Externalizing disorders Measurement Diagnostic and Statistical Manual of Mental Disorders International Classification of Diseases Hierarchical Taxonomy of Psychopathology Psychopathology

Contributions to data transmission at critical moments on unmanned aerial vehicles

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Chinese Journal of Aeronautics 2023年第8期36卷 247-257页

作者： Jose Enrique RODRIGUEZ David SEGURADO manuel sanchez Jose Javier MARTINEZ Rafael GONZALEZ National Institute of Aerospace Technology Madrid 28850Spain Department of Computer Science University of AlcalaAlcala de HenaresMadrid 28054Spain

As for unmanned aircraft, the knowledge of the aircraft performance is directly related with the navigation, guidance, and control system programming. Therefore, the measured data in each phase of the flight must be sufficiently precise to obtain a good characterization of *** article proposes new methods of sending information to ground, which make it possible to know the aircraft behavior accurately, and for this purpose, four contributions have been made for ALO(Avión Ligero de Observación, Spanish acronym for Light Observation Aircraft). Currently, the characterization is based on data obtained at ten samples per second, insufficient to acquire detailed knowledge of what happened during the whole flight of an aircraft. As a result of these contributions, many more samples per second of accelerations and angular velocities are obtained at the most critical moments of the flight, such as takeoff or landing. Among the improvements included are data compression techniques, providing references to locate the measured data in time and identifying labels of each parameter.

关键词： Critical moments Data transmission Datacompression tech-niques Radio-modem Recommended Standard 232(RS-232) Unmannedaerial vehicle

The RNA binding proteins TIA1 and TIAL1 promote Mcl1 mRNA translation to protect germinal center responses from apoptosis

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Cellular & Molecular Immunology 2023年第9期20卷 1063-1076页

作者： Ines COsma-Garcia Mailys Mouysset Dunja Capitan-Sobrino Yann Aubert Martin Turner manuel D.diaz-Muñoz Institute for Infectious and Inflammatory Diseases(INFINITy) Inserm UMR1291CNRS UMR5051University Paul SabatierCHU PurpanToulouse 31024France Immunology Program The Babraham InstituteCambridgeUK

Germinal centers(GCs)are essential for the establishment of long-lasting antibody *** B cells rely on post-transcriptional RNA mechanisms to translate activation-associated transcriptional programs into functional changes in the cell ***,the critical proteins driving these key mechanisms are still ***,we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC ***1-and TIAL1-deficient GC B cells fail to undergo antigen-mediated positive selection,expansion and differentiation into B-cell clones producing high-affinity ***,TIA1 and TIAL1 control the transcriptional identity of dark-and light-zone GC B cells and enable timely expression of the prosurvival molecule ***,we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells.

关键词： Adaptive immunity Germinal centers Post-transcriptional gene regulation RNA binding proteins Cell identity Apoptosis/10.1038/s41423-023-01063-4

The influence of nanotopography on cell behaviour through interactions with the extracellular matrix-A review

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Bioactive Materials 2022年第9期7卷 145-159页

作者： Jiajun Luo Matthew Walker Yinbo Xiao Hannah Donnelly Matthew J.Dalby manuel Salmeron-sanchez Centre for the Cellular Microenvironment University of GlasgowGlasgowG128LTUK

Nanotopography presents an effective physical approach for biomaterial cell manipulation mediated through material-extracellular matrix *** extracellular matrix that exists in the cellular microenvironment is crucial for guiding cell behaviours,such as determination of integrin ligation and interaction with growth *** interactions with the extracellular matrix regulate downstream mechanotransductive pathways,such as rearrangements in the cytoskeleton and activation of signal *** adsorption onto nanotopography strongly influences the conformation and distribution density of extracellular matrix and,therefore,subsequent cell *** this review,we first discuss the interactive mechanisms of protein physical adsorption on ***,we summarise advances in creating nanotopographical features to instruct desired cell ***,we focus on the cellular mechanotransductive pathways initiated by *** review provides an overview of the current state-of-the-art designs of nanotopography aiming to provide better biomedical materials for the future.

关键词： Nanotopography Cell-material interaction Protein adsorption Biomaterials

Functional explanation of extreme hatching asynchrony: Male Manipulation Hypothesis

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Zoological Research 2022年第5期43卷 843-850页

作者： manuel Soler Francisco Ruiz-Raya Lucia sanchez-Perez Juan Diego Ibanez-Álamo Juan JoseSoler Departamento de Zoologia Facultad de CienciasUniversidad de GranadaGranada E-18071Spain Unidad Asociada Coevolucion:Cucos Hospedadores y Bacterias SimbiontesUniversidad de GranadaGranada E-18071Spain Centro de Investigacion Mariña Universidade de VigoGrupo de Ecoloxia AnimalVigo 36310Spain Departamento de Ecologia Funcional y Evolutiva Estacion Experimental de ZonasÁridas(CSIC)Almeria E-4120Spain

Hatching asynchrony in birds is considered an adaptation to facilitate brood reduction because under conditions of food scarcity, the smallest nestling usually dies soon after hatching, thereby minimizing parental effort. However, in species with extreme hatching asynchrony, the last hatchlings paradoxically experience a very low probability of survival and death can take so long that it can hardly be considered an adaptation. Here, we propose and experimentally tested a new adaptive hypothesis explaining the brood reduction paradox, namely the“Male Manipulation Hypothesis”. Our hypothesis suggests that by inducing asynchronous hatching,females increase the feeding requirements of the brood, which will induce males to increase provisioning effort. In addition, females may extend the period of male manipulation by feeding the smallest nestling just enough to sustain life. Our study showed that male common blackbirds(Turdus merula) increased their effort(i.e., number of food items per hour) in experimental asynchronous broods compared to synchronous broods, while females reduced their contribution, as predicted by the hypothesis.

关键词： Brood reduction Food allocation Hatchingasynchrony Malemanipulation hypothesis Sexual differences in food allocation Turdus merula