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Effect of long-term fertilization on bacterial communities in wheat endosphere

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Pedosphere 2021年第4期31卷 538-548页

作者： yuying ma Pamela WEISENHORN Xisheng GUO Daozhong WANG Teng YANG Yu SHI Huanchao ZHANG Haiyan CHU Co-Innovation Center for the Sustainable Forestry in Southern China College of ForestryNanjing Forestry UniversityNanjing 210037(China) State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Biosciences Division Argonne National LaboratoryArgonneIL 60439(USA) Key Laboratory of Nutrient Cycling and Resources Environment of Anhui Province Soil and Fertilizer Research InstituteAnhui Academy of Agricultural SciencesHefei 230031(China) University of Chinese Academy of Sciences Beijing 100049(China)

Fertilization has been shown to exert a significant influence on soil microorganisms and directly and indirectly influences plant growth and survival in agroecosystems. However, it is unknown whether fertilization affects endophytic microbial communities, which are ubiquitous and intimately associated with plant growth and health. Herein, we investigated endophytic bacterial communities in wheat leaves and roots under different long-term fertilization regimes,including NPK chemical fertilizer and NPK chemical fertilizer combined with wheat straw, pig manure, or cow manure. Endophytic bacterial community composition considerably differed in leaves and roots. Although different fertilization treatments did not affect the endophytic bacterial species richness or phylogenetic diversity in either leaves or roots, the community composition was significantly altered, particularly in roots. The endophytic bacterial co-occurrence network in leaves was more complex and stable than that in roots. Furthermore, many of the keystone species that were identified by their topological positions in the co-occurrence networks of leaves and roots were involved in plant growth and fitness. The total relative abundance of keystone species was the highest in the NPK plus cow manure treatment in both leaves and roots. Overall, our results suggest that different fertilization regimes can strongly affect endophytic bacterial communities, and the combination of NPK fertilizer and cow manure promoted the relative abundance of the key endophytic bacterial microbiota in both leaves and roots, which might be beneficial for plants in agroecosystems.

关键词： co-occurrence network endophytic bacterial community key microbiota long-term fertilization organic matter

Adsorption and Visible Light Photocatalytic Degradation of Electrospun PAN@W_(18)O_(49) Nanofibers

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Chemical Research in Chinese Universities 2021年第3期37卷 428-435页

作者： ma yuying HE Dayong LIU Jiadi WANG Yuannan YANG Mei WANG Hao QIU Ju LI Wenyan LI Yongxin WANG Ce Alan G.macDiarmid Institute College of ChemistryJilin UniversityChangchun130012P.R.China College of Plant Science Jilin UniversityChangchun130062P.R.China College of New Energy and Environment Jilin UniversityChangchun130021P.R.China

Low density and high porosity polyacrylonitrile(PAN)nanofibers prepared by electrospinning were used as brackets for photocatalyst W_(18)O_(49) to prepare a kind of light weight and easy recycling water purification material for the first *** influence of tungsten source concentration on the formation of W_(18)O_(49) during a solvothermal process was systematically *** prepared PAN@W_(18)O_(49) nanofibers(NFs)utilize the outstanding visible light photocatalytic performance and the adsorption performance of W_(18)O_(49),and at the same time give the advantages of low density and easy *** pollutant removal performance of the composite nanofibers was investigated by using five contaminants including rhodamine B(RhB),methylene blue(MB),malachite green(MG),methyl orange(MO)and chlortetracycline(CTC)as *** them,the degradation process of rhodamine B has been studied in *** five cycles,the degradation efficiency did not decrease significantly,showing excellent reusability of PAN@W_(18)O_(49) ***,the adsorption performance of PAN@W_(18)O_(49) NFs during the photocatalytic process was also studied in *** with recently reported literature,the degradation efficiency of organic pollutants by PAN@W_(18)O_(49) NFs showed better performance,and PAN@W_(18)O_(49) NFs felt had a promising prospect in the field of degradation of contaminants.

关键词： Photocatalysis Adsorption Electrospinning Solvothermal reaction Dye degradation

Determination of atractylon in rat plasma by a GC-MS method and its application to a pharmacokinetic study

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Journal of Pharmaceutical Analysis 2015年第5期5卷 327-331页

作者： Han Yan Yuanyuan Sun yuying ma Bin Ji Xiaohong Hou Zhiguo Yu Yunli Zhao School of Pharmacy Shenyang Pharmaceutical University School of Pharmaceutical Engineering Shenyang Pharmaceutical University

A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.

关键词： AtractylonAtractylodisRat plasmaPharmacokineticsGC-MS

Decreased levels of phosphorylated synuclein in plasma are correlated with poststroke cognitive impairment

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Neural Regeneration Research 2025年第9期20卷 2598-2610页

作者： Yi Wang Yuning Li Yakun Gu Wei ma yuying Guan Mengyuan Guo Qianqian Shao Xunming Ji Jia Liu Department of Clinical Laboratory Beijing Bo’ai HospitalChina Rehabilitation Research CenterCapital Medical UniversityBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Science and TechnologyCollaborative Innovation Center for Brain DisordersBeijing Advanced Innovation Center for Big Data-based Precision MedicineCapital Medical UniversityBeijingChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina

Poststro ke cognitive impairment is a major secondary effect of ischemic stroke in many patients;however,few options are available for the early diagnosis and treatment of this *** aims of this study were to(1)determine the specific relationship between hypoxic andα-synuclein during the occur of poststroke cognitive impairment and(2)assess whether the serum phosphorylatedα-synuclein level can be used as a biomarker for poststro ke cognitive *** found that the phosphorylatedα-synuclein level was significantly increased and showed pathological aggregation around the cerebral infa rct area in a mouse model of ischemic *** addition,neuronalα-synuclein phosphorylation and aggregation were observed in the brain tissue of mice subjected to chronic hypoxia,suggesting that hypoxia is the underlying cause ofα-synuclein-mediated pathology in the brains of mice with ischemic *** phosphorylatedα-synuclein levels in patients with ischemic stroke were significantly lower than those in healt hy subjects,and were positively correlated with cognition levels in patients with ischemic ***,a decrease in serum high-density lipoprotein levels in stroke patie nts was significantly correlated with a decrease in phosphorylatedα-synuclein *** ischemic stroke mice did not show significant cognitive impairment or disrupted lipid metabolism 14 days after injury,some of them exhibited decreased cognitive function and reduced phosphorylatedα-synuclein *** together,our results suggest that serum phosphorylatedα-synuclein is a potential biomarker for poststroke cognitive impairment.

关键词： biomarker high-density lipoprotein ischemic stroke phosphorylatedα-synuclein poststroke cognitive impairment

Perilipin 5 regulates hepatic stellate cell activation and high-fat diet-induced non-alcoholic fatty liver disease

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Animal Models and Experimental Medicine 2024年第2期7卷 166-178页

作者： Xuecui Yin Lin Dong Xiaohan Wang Zhenzhen Qin yuying ma Xiaofei Ke Ya Li Qingde Wang Yang Mi Quanjun Lyu Xia Xu Pengyuan Zheng Youcai Tang Department of Internal Medicine the Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Pediatrics the Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Clinical Nutrition the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education of ChinaCo-innovation Center of Henan Province for New drug R&D and Preclinical SafetySchool of Pharmaceutical SciencesZhengzhou UniversityZhengzhouChina Department of Pediatrics GastroenterologyHenan Key Laboratory of Rehabilitation MedicineHenan Joint International Research Laboratory of Chronic Liver Injury and Henan Provincial Outstanding Overseas Scientists Chronic Liver Injury Studiothe Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases *** stellate cells(HSCs)are the major effector cells of liver *** contain abundant lipid droplets(LDs)in their cytoplasm during *** 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid ***,little is known about the role of PLIN 5 in HSC ***:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus *** the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in *** corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy *** analysis of mice liver tissue metabolism was performed based on UPLC-MS/***,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and ***:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK *** addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver ***:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.

关键词： AMPK apoptosis hepatic stellate cell liver fibrosis perilipin 5

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GR－35双基推进剂工艺性能的研究

推进技术 1995年第4期16卷 66-70页

作者：马玉英山西兴安化学材料厂

ＧＲ－３５双基推进剂在其配方组成中，硝化甘油含量较高，固体颗粒含量较多，至使该推进剂的机械感度较高，这给药料进行螺旋挤压带来了一定的困难。合理地选择工艺条件，既保证了该推进剂安全、顺利进行挤压成型，而且又能压制出不同... 详细信息

ＧＲ－３５双基推进剂在其配方组成中，硝化甘油含量较高，固体颗粒含量较多，至使该推进剂的机械感度较高，这给药料进行螺旋挤压带来了一定的困难。合理地选择工艺条件，既保证了该推进剂安全、顺利进行挤压成型，而且又能压制出不同形状的药型，满足了多种武器的需要。

关键词：双基推进剂力学性质压制成形工艺性能

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纤维沥青胶浆流变性能的试验研究

公路工程 2014年第5期39卷 311-314页

作者：马煜缨陕西省交通规划设计研究院陕西西安710068

为考察纤维沥青胶浆的高温性能和流变性能,对掺不同种类和剂量纤维的沥青胶浆进行不同温度下的粘度试验和动态剪切流变试验,分析纤维沥青胶浆的粘度、复数模量、相位角和抗车辙因子的变化规律,同时分析粘度与抗车辙因子的相关性。研究... 详细信息

为考察纤维沥青胶浆的高温性能和流变性能,对掺不同种类和剂量纤维的沥青胶浆进行不同温度下的粘度试验和动态剪切流变试验,分析纤维沥青胶浆的粘度、复数模量、相位角和抗车辙因子的变化规律,同时分析粘度与抗车辙因子的相关性。研究结果表明,纤维对沥青胶浆有明显的增粘与增弹作用,其中聚酯纤维最大,其次为木质素纤维,矿物纤维最弱;随着纤维掺量的增大,纤维增粘和增弹作用逐步发挥,当掺量达到沥青质量的0.04%时,纤维沥青胶浆的粘度、复数模量和抗车辙因子显著增大而相位角显著降低;纤维沥青胶浆的粘度与抗车辙因子存在良好的线性关系。

关键词：道路工程沥青胶浆纤维粘度流变性能

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道家思想在美国的百年传播研究

东南学术 2023年第1期 214-223页

作者：马钰滢蒋述卓暨南大学文学院

在20世纪中国与西方、传统与现代碰撞融通的学术语境下,道家思想成为中国文化走向世界的重要传译中介和“形象大使”。大批中西方学者通过对道家思想的译介和阐释来为其理论寻找据点,尤以美国最为突出。道家思想在美国的传播历程可划分... 详细信息

在20世纪中国与西方、传统与现代碰撞融通的学术语境下,道家思想成为中国文化走向世界的重要传译中介和“形象大使”。大批中西方学者通过对道家思想的译介和阐释来为其理论寻找据点,尤以美国最为突出。道家思想在美国的传播历程可划分为三个阶段:附会与比较(19世纪末至20世纪30年代)、阐释与借鉴(20世纪40年代至60年代)、对话与互显(20世纪70年代至90年代末)。通过厘清道家思想在美国的百年传播史,呈现其接受语境、基本特征与变化趋势,可以彰显中国传统文化参与中西文明互鉴的意义。

关键词：道家思想英译传播美国汉学

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提高原木出材率的工艺措施

林业科技 2003年第3期28卷 44-45页

作者：马玉英黑龙江省穆棱林业局宏林木制品厂穆棱镇153071

生产实践经验表明 ,通过科学下锯、合理截断、合理使用锯条及科学合理地拼接板材等工艺措施 ,可以大幅度提高原木出材率 ,达到节约资源。

关键词：原木出材率工艺措施下锯截断拼接制材