Determination of atractylon in rat plasma by a GC-MS method and its application to a pharmacokinetic study
Determination of atractylon in rat plasma by a GC-MS method and its application to a pharmacokinetic study作者机构:School of PharmacyShenyang Pharmaceutical University School of Pharmaceutical EngineeringShenyang Pharmaceutical University
出 版 物:《Journal of Pharmaceutical Analysis》 (药物分析学报(英文版))
年 卷 期:2015年第5卷第5期
页 面:327-331页
学科分类:1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学]
基 金:supported by the National Natural Science Foundation of China(Nos.81073143 and 81373926) Research Fund for the Doctoral Program of Higher Education of China(No. 20092134110004)
主 题:AtractylonAtractylodisRat plasmaPharmacokineticsGC MS
摘 要:A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry (GC- MS) was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane (1:1, v/v) using acetophenone as an internal standard (IS). Analytes were determined in selective ion monitoring (SIM) mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.