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iTRAQ-based quantitative proteomic analysis reveals key pathways responsible for scurs in sheep(Ovis aries)

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Journal of Integrative Agriculture 2018年第8期17卷 1843-1851页

作者： HE Xiao-hong CHEN Xiao-fei PU Ya-bin GUAN Wei-jun SONG Shen ZHAO Qian-jun LI Xiang-chen JIANG Lin ma yue-hui Institute of Animal Science Chinese Academy of Agricultural Sciences CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources Institute of Animal Science Chinese Academy of Agricultural Sciences Institute of Animal Science Enshi Autonomous Prefecture Academy of Agricultural Sciences

Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.

关键词： scurs sheep iTRAQ horn development ECM-receptor interaction pathway

Genetic Variation of EPAS1 Gene in Tibetan Pigs and Three Low-Altitude Pig Breeds in China

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Journal of Integrative Agriculture 2014年第9期13卷 1990-1998页

作者： DONG Kun-zhe KANG Ye YAO Na SHU Guo-tao ZUO Qing-qing ZHAO Qian-jun ma yue-hui Key Laboratory of Farm Animal Genetic Resources and Utilization Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences College of Animal Science and Technology Yunnan Agricultural University

Endothelial PAS domain protein 1（EPAS1）, also called hypoxia-inducible factor-2, is a key regulatory factor of hypoxic responses and plays an essential role in high-altitude adaptation in mammalian species. In this study, polymorphisms of EPAS1 were detected in 217 individuals from 2 Tibetan pig populations and 3 low-altitude pig breeds by DNA pooling, PCR-SSCP, PCR-RFLP and DNA sequencing methods. A total of 14 synonymous polymorphisms were identified in the coding region. The analysis suggested that SNP1（G963A）, SNP7（C1632T）, SNP10（G1929A） and SNP11（G1947A） showed potential association with high-altitude environment because of their particular variation patterns in Tibetan pigs. Linkage disequilibrium（LD） of these SNPs was analyzed. One common LD block including 5 SNPs clustering in exon 12 was identified in all studied pig populations. Haplotype H1（AGGTC） in LD block was dominant in Tibetan pigs（76.6 and 74.2% in Linzhi（LZ） and Chayu（CY） pigs, respectively） and segregated at higher frequency than that in low-altitude pig breeds（52.3, 58.7 and 56.2% in Wuzhishan（WZS）, Min（M） and Laiwu（LW） pigs, respectively）, indicating that H1 may relate to adaptation to high altitude in Tibetan pigs. These findings raise hope that EPAS1 gene can be a candidate gene that involved in adaptation of high altitude in Tibetan pigs.

关键词： pig EPAS1 gene SNP haplotype nature selection

Investigation of mitochondrial DNA genetic diversity and phylogeny of goats worldwide

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Journal of Integrative Agriculture 2022年第6期21卷 1830-1837页

作者： GUO Yi GONG Ying HE Yong-meng YANG Bai-gao ZHANG Wei-yi CHEN Bo-er HUANG Yong-fu ZHAO Yong-ju ZHANG Dan-ping ma yue-hui CHU Ming-xing E Guang-xin College of Animal Science and Technology Southwest UniversityChongqing 400715P.R.China Chongqing Agriculture and Rural Affairs Committee of Tongnan Chongqing 402660P.R.China Dazhou Animal Husbandry Technology Extension Station Dazhou 635299P.R.China Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

Genetic diversity,population structure,and population expansion of goats worldwide(4165 individuals from 196 breeds)were analyzed using published mitochondrial DNA(mtDNA)D_loop hypervariable region *** showed that 2409 haplotypes and 301 polymorphic sites were present within the 401-bp length D_loop region,the nucleotide diversity(Pi)was 0.03471,and the haplotype diversity(Hd)was *** analysis revealed that 98.92%of haplotypes were divided into six obvious clusters,consistent with the classification of the known mitochondrial haplogroups of *** A accounted for the largest proportion(86%).Interestingly,two unknown divisions(Unknown I and Unknown II)were discovered from goats in Southwest China,suggesting that Southwest China has unique maternal *** of molecular variance(AMOVA)and the average number of pairwise differences between populations(PiXY)indicated that geographical variation was small but *** tests(Tajima’s D and Fu’s FS tests)and mismatch distribution showed that haplogroups B,C,and G had expansion *** addition,the phylogenetic relationship between domestic and wild goats suggested that Capra aegagrus is the most likely wild ancestor and may have participated in the domestication of ancestral populations of A,B,C,and F haplogroups.A meta-analysis on the mtDNA sequences of goats from international databases was conducted to analyze goats’genetic diversity,population structure,and matrilineal system evolution *** results may help further understand the domestication history and gene flow of goats worldwide.

关键词： mitochondrial DNA genetic diversity population structure population expansion phylogeny

Analysis of SNPs in the Promoter Region of the Growth Hormone(GH) Gene in Mini-pigs

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畜牧兽医学报 2010年第S1期41卷 26-30页

作者： ZHENG mao-en PAN Deng-ke FENG Shu-tang LIU Xiao YE Shao-hui PU Ya-bin HE Xiao-hong ZHAO Qian-jun GUAN Wei-jun ma yue-hui Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China College of Animal Science and Technology Yunnan Agricultural UniversityKunming 650201China Lanzhou Institute of Animal and Veterinary Pharmaceutics Science Chinese Academy of Agricultural SciencesLanzhou 730050China Shandong Laboratory Animal Center Ji’nan 250002China

Single nucleotide polymorphisms(SNPs) of the growth hormone(GH) gene were investigated in six pig breeds,consisting of four mini-pig breeds(Wuzhishan,Bama,Xiang and Tibet pig),and two others(Dahlan and Landrace pig).Three pairs of primers for promoter regions of the GH gene were designed on the basis of the pig genomic sequence and SNPs were detected by the PCR-SSCP *** results indicated three mutations in the 5’-flanking *** analysis results showed that the frequencies of allele A and D in four mini-pig breeds were higher than that in other breeds at a locus within the 5’-flanking region(Pmay be associated with these SNPs of 5’-flanking region and amino acid mutation of the signal peptide of GH in these pig breeds.

关键词： minipigs growth hormone promoter region dwarf

Ni50Ti30Zr20与Ni50Ti50合金摩擦磨损性能对比研究

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表面技术 2019年第12期48卷 204-210,225页

作者：薛贤达马悦辉李岩北京航空航天大学材料科学与工程学院北京100191 兰州西脉记忆合金股份有限公司兰州730010

目的以Ni50Ti50合金为参照物,研究Ni50Ti30Zr20合金的摩擦磨损性能。方法采用非自耗真空电弧熔炼炉炼制Ni50Ti30Zr20合金,在合金铸锭上切取样品,以Ni50Ti50合金为对比样品,通过能谱测试(EDS)、X射线衍射(XRD)、显微硬度(HV)测试、摩擦... 详细信息

目的以Ni50Ti50合金为参照物,研究Ni50Ti30Zr20合金的摩擦磨损性能。方法采用非自耗真空电弧熔炼炉炼制Ni50Ti30Zr20合金,在合金铸锭上切取样品,以Ni50Ti50合金为对比样品,通过能谱测试(EDS)、X射线衍射(XRD)、显微硬度(HV)测试、摩擦磨损测试、扫描电镜测试(SEM)和3D形貌测试,分别评价Ni50Ti30Zr20合金与Ni50Ti50合金的成分、相组成、显微硬度、耐磨性、磨痕形貌和磨损体积。结果XRD结果显示,Ni50Ti50和Ni50Ti30Zr20合金室温分别由B2奥氏体和B19’马氏体相组成。显微硬度测试结果表明,Ni50Ti30Zr20和Ni50Ti50合金的显微硬度值分别为(381.64±7.32)HV和(230.58±6.74)HV。从形貌图可以看出,两种合金磨损后,磨痕形貌主要由剥层组成。从磨痕能谱分析得出,摩擦实验后,样品表面O和Si元素含量明显增加。根据摩擦系数曲线和磨痕三维形貌图发现,同样载荷下,Ni50Ti30Zr20合金的摩擦系数和磨损体积均小于Ni50Ti50合金,在载荷为20 N时,Ni50Ti30Zr20合金的磨损体积为0.078 mm^3,Ni50Ti50合金的磨损体积为0.084 mm^3。结论Ni50Ti30Zr20与Ni50Ti50合金的磨损机制均为疲劳磨损,两种合金在摩擦实验过程中均会发生氧化,同时磨球会有部分剥落到合金磨痕剥层中。Ni50Ti30Zr20合金的耐磨性优于Ni50Ti50合金。

关键词： Ni50Ti30Zr20 形状记忆合金相组成显微硬度耐磨性磨损机制

Hypidone hydrochloride(YL-0919)ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation

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Neural Regeneration Research 2025年第8期20卷 2325-2336页

作者： Yafan Bai hui ma yue Zhang Jinfeng Li Xiaojuan Hou Yixin Yang Guyan Wang Yunfeng Li Department of Anesthesiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Beijing Institute of Basic Medical Sciences BeijingChina Hebei North University ZhangjiakouHebei ProvinceChina Beijing Institute of Pharmacology and Toxicology State Key Laboratory of Toxicology and Medical CountermeasuresBeijing Key Laboratory of NeuropsychopharmacologyBeijingChina

Traumatic brain injury involves complex pathophysiological mechanisms,among which oxidative stress significantly contributes to the occurrence of secondary *** this study,we evaluated hypidone hydrochloride(YL-0919),a self-developed antidepressant with selective sigma-1 receptor agonist properties,and its associated mechanisms and targets in traumatic brain *** experiments to assess functional deficits were followed by assessment of neuronal damage through histological analyses and examination of blood-brain barrier permeability and brain ***,we investigated the antioxidative effects of YL-0919 by assessing the levels of traditional markers of oxidative stress in vivo in mice and in vitro in HT22 ***,the targeted action of YL-0919 was verified by employing a sigma-1 receptor antagonist(BD-1047).Our findings demonstrated that YL-0919 markedly improved deficits in motor function and spatial cognition on day 3 post traumatic brain injury,while also decreasing neuronal mortality and reversing blood-brain barrier disruption and brain ***,YL-0919 effectively combated oxidative stress both in vivo and in *** protective effects of YL-0919 were partially inhibited by *** results indicated that YL-0919 relieved impairments in motor and spatial cognition by restraining oxidative stress,a neuroprotective effect that was partially reversed by the sigma-1 receptor antagonist ***-0919 may have potential as a new treatment for traumatic brain injury.

关键词： antidepressant drug blood-brain barrier cognitive function hypidone hydrochloride(YL-0919) neurological function nuclear factor-erythroid 2 related factor 2 oxidative stress sigma-1 receptor superoxide dismutase traumatic brain injury

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中小学教师流动中权益保障的对策分析

江西教育科研 2007年第6期 42-43页

作者：麻跃辉云南师范大学教育科学与管理学院

当前,中小学教师的流动已逐渐频繁起来,为了保障中小学教师在流动中的权益,首先必须从立法的高度确定教师有"自主流动权"。同时,政府应该采取措施对这种权力予以保障。如,增加农村教育投入,建立个别化教师工资系统,建立健全教师人才流... 详细信息

当前,中小学教师的流动已逐渐频繁起来,为了保障中小学教师在流动中的权益,首先必须从立法的高度确定教师有"自主流动权"。同时,政府应该采取措施对这种权力予以保障。如,增加农村教育投入,建立个别化教师工资系统,建立健全教师人才流动市场等。

关键词：中小学教师流动权益保障对策

绵羊Cytb基因序列多态性及系统进化研究(英文)

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Acta Genetica Sinica 2006年第12期33卷 1081-1086页

作者：王昕马月辉陈宏西北农林科技大学动物科技学院杨凌712100 中国农业科学院畜牧研究所北京100094

以8个中国地方绵羊品种和1个外来品种的20个个体为研究对象,通过对Cytb基因的全序列测定,结果表明:绵羊的单倍型多样性为97.1%。所有序列的平均碱基组成为27.1%T,28.5%C,31.4%A及13.0%G,G+C含量为41.5%,核苷酸多样性为0.602%。在所有序... 详细信息

以8个中国地方绵羊品种和1个外来品种的20个个体为研究对象,通过对Cytb基因的全序列测定,结果表明:绵羊的单倍型多样性为97.1%。所有序列的平均碱基组成为27.1%T,28.5%C,31.4%A及13.0%G,G+C含量为41.5%,核苷酸多样性为0.602%。在所有序列中共检测到43个变异位点,其中包括40处转换和3处颠换。Fu’s中性检验表明差异不显著(0.10>P>0.05),说明绵羊群体未发生群体扩张事件。NJ、ME及UPGma聚类结果均表明,我国绵羊可分为3个单倍型组,这提示我国绵羊有3个母系起源。

关键词：绵羊 Cyt 6 系统进化

Treatment of skull base communicating tumor with endoscopeassisted microneurosurgery and diode laser

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Chinese Medical Journal 2007年第4期120卷 342-344页

作者： PAN Jian-wei ZHAN Ren-ya TONG Ying ZHENG Jie-sheng WAN Shu HUANG Hong-guang ma yue-hui ZHOU Yong-qing Department of Neurosurgery First Affiliated Hospital MedicalCollege of Zhejiang University Hangzhou 310003 China

The skull base communicating tumor is characterized by simultaneous invasion of the intracranial and extracranial structures. The tumor may originate from the intracranial structures and invade extracranially, or vice versa. The total resection of the tumor is difficult, and a lot of complications may occur after the operation.1 From September 1998 to September 2002, 21 patients with skull base communicating tumors were treated in our hospital by endoscope-assisted combined with diode laser through approaches. microneurosurgery different operative

关键词： skull base communicating tumor endoscope-assisted microneurosurgery diode laser