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Identification and expression analysis of chlorogenic acid biosynthesis key gene PpHCT in peach

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Horticultural Plant Journal 2023年第4期9卷 670-680页

作者： Ziwen Su Meng Sun Zhixiang Cai Shaolei Guo Jiyao Li Bintao Zhao Zhijun Shen ruijuan ma Juan Yan Mingliang Yu College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu 210095China Institute of Pomology Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjingJiangsu 210014China

Shikimic acid/quinic acid hydroxy cinnamyl transferase(HCT)is one of the key enzymes in the phenylpropanoid ***,the role of the HCT gene in chlorogenic acid(CGA)biosynthesis in peach fruit remains *** this,we identified the accumulation pattern of CGA in four peach cultivars,cloned and characterized 11 PpHCT gene members,and further analyzed the expression patterns of these PpHCT genes during fruit *** contents of CGAs in the four peach cultivars all exhibited a trend of increasing and then decreasing during the fruit growth and ***,the contents of CGAs in the peel and flesh were *** structure analysis indicated that the PpHCT genes were highly conserved,containing two exons and one *** protein structure analysis demonstrated that the PpHCT proteins contained two conserved motifs(HXXXD,DFGWG)and a transferase domain(PF02458),which belonged to the BAHD acyltransferase *** cis-acting element analysis suggested that the promoters of PpHCT genes contained many light-related,hormone-related,stress-related,tissue-specific,and circadian-related elements,and they could participate in a variety of biological *** analysis showed that the HCT proteins of peach were closely related to the HCT proteins of plum and had a close evolutionary *** qRT-PCR analysis indicated that the expression levels of PpHCT1 and PpHCT2 showed an opposite trend to the accumulation of CGA,whereas the expression levels of PpHCT4,PpHCT5,PpHCT7,PpHCT8,and PpHCT11 demonstrated the same trend as CGA *** was worth noting that only PpHCT4 and PpHCT5 were highly expressed in the two high-CGA cultivars but showed low levels of expression in the two low-CGA ***,it was hypothesized that these two genes might be key genes to the synthesis of CGA in peach *** findings provide a theoretical basis for further study on the biological functions of the HCT gene and help to

关键词： Prunus persica(L.) Chlorogenic acid PpHCT Bioinformatics analysis Gene expression

Effect of Bag Removing with Reflective Film Mulching Before Harvest on Fruit Coloration and Expression of Anthocyanin Related Genes in Peach

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Horticultural Plant Journal 2015年第3期1卷 139-146页

作者： ZHANG Binbin ma ruijuan ZHANG Chunhua CAI Zhixiang YAN Zhimei Institute of Horticulture Jiangsu Academy of Agricultural Sciences Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement

Appearance quality is the main element forming the fruit quality. Both bagging and reflective film application can improve appearance comprehensive quality. In order to determine the effects of bagging and reflective film on peach fruit coloration, this study explored the effects of applying reflective film before harvest on fruit appearance quality of peach(Prunus persica), and discussed the influencing mechanism on fruit coloration. Yellow outside and black inside double-layer bags were removed from fruit twelve days d before harvest. Peel color, pigment content, enzyme activity as well as the gene expression related to fruit coloration of a well-colored and late-ripening peach cultivar ‘Xiahui 8' with different treatments(non-bagging with non-reflective film mulching, debagging with non-reflective film mulching, debagging with reflective film mulching) were researched. The results showed that both debagging with non-reflective film mulching and debagging with reflective film mulching could significantly improve red and green color difference value(a*), a*/b*(b* is yellow and blue color difference value), chroma(C), anthocyanin(Ant) content and reduce hue angle(h), while debagging with reflective film mulching had higher Ant content than debagging with non-reflective film mulching(P < 0.05). The two treatments up-regulated the transcript level of anthocyanin biosynthetic related genes(UFGT, CHS) at the beginning of the experiment, however, the expression of DFR, LDOX and F3 H were enhanced along with the testing process. A downward trend on peel phenylalanine ammonia lyase(PAL) activity of non-bagging with non-reflective film mulching was observed during the experiment, however, the peel PAL activity of the other two treatments first increased and then decreased. What's more, peel PAL activity of debagging with reflective film mulching was higher than debagging with non-reflective film mulching. These results suggested that Ant synthesis in the peel was the result

关键词： peach coloration reflective film color difference pigment gene expression

Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures

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Horticultural Plant Journal 2016年第2期2卷 75-81页

作者： YANG Yong ma ruijuan ZHANG Binbin SONG Zhizhong ZHANG Chunhua GUO Shaolei YU Mingliang Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Institute of Horticulture Jiangsu Academy of Agricultural Sciences College of Horticulture Nanjing Agricultural University

The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui' and ‘Yumyeong', which are stonyhard, ‘Yinhualu', which is softmelting, ‘Hujing Milu', which is hard-melting, and ‘Baby Gold 6', which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong' and ‘Xiacui' under either a room temperature(25 °C) or low temperature(4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong' fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however,was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously upregulated in ‘Yumyeong', but not in ‘Xiacui' peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures.

关键词： Prunus persica ethylene biosynthetic pathway soften gene expression

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基于语旨理论的会话幽默分析

河南科技学院学报（社会科学版） 2012年第5期32卷 70-72页

作者：马睿娟济南大学外国语学院山东济南250022

幽默是人类特有的一种语言现象,其特点决定了它是一门多学科性研究。文章结合系统功能语言学语域理论中的语旨变量,将马丁语旨三分法模式作为文章的理论框架,以著名美国家庭情景喜剧《成长的烦恼(第一季)》为语料,分别从情感类型、权势... 详细信息

幽默是人类特有的一种语言现象,其特点决定了它是一门多学科性研究。文章结合系统功能语言学语域理论中的语旨变量,将马丁语旨三分法模式作为文章的理论框架,以著名美国家庭情景喜剧《成长的烦恼(第一季)》为语料,分别从情感类型、权势高低、情感作用三个角度,探讨语旨对幽默生成的影响。通过对语料的分析、解释,揭示出情感类型的偏离、权势高低的违背以及情感作用的变异都会影响幽默效果的表达。

关键词：语旨会话幽默《成长的烦恼》

响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析

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食品工业科技 2024年第7期45卷 217-226页

作者：朱清清马瑞娟陈剑锋谢友坪福建省海产品废弃物综合利用工程技术研究中心福州市海产品高值化利用行业技术创新中心福州大学福建福州350108

为实现鱿鱼加工下脚料鱿鱼皮的高值化利用,本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数(Trichloroacetic acid-nitrogen soluble index,TCA-NSI)为评价指标,采用单因素和响应面试验优化酶解工艺参数,并对所制备... 详细信息

为实现鱿鱼加工下脚料鱿鱼皮的高值化利用,本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数(Trichloroacetic acid-nitrogen soluble index,TCA-NSI)为评价指标,采用单因素和响应面试验优化酶解工艺参数,并对所制备蛋白肽的氨基酸组成、分子量分布、蛋白肽序列、抗氧化活性及其体外模拟消化特性进行表征。结果表明,最佳酶解工艺条件为酶解温度43.50℃、酶解时间100 min、酶解pH7.50、胰蛋白酶与碱性蛋白酶比例2:1、加酶量4000 U/g,在此条件下其TCA-NSI可达89.02%±0.66%。所制备的鱿鱼皮蛋白肽其氨基酸组成均衡,必需氨基酸指数(Essential amino acid index,EAAI)可达0.90。此外,鱿鱼皮蛋白肽含有43个多肽序列,其中38个肽段的分子质量低于1800 Da,其DPPH自由基、ABTS^(+)自由基和羟自由基清除率的IC_(50)分别为0.61、0.28和1.95 mg/mL,且经体外模拟胃肠消化后仍能维持较好的抗氧化活性。本研究结果为鱿鱼加工下脚料的开发利用和精深加工提供了理论依据。

关键词：鱿鱼皮蛋白肽理化性质抗氧化活性体外模拟消化

同方期刊数据库博看期刊评论

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BIM技术在工程造价控制中的应用

电子技术（上海） 2022年第7期51卷 154-156页

作者：马睿涓甘肃建筑职业技术学院甘肃730050

阐述建筑工程造价中的控制与管理状况,分析BIM技术的应用,提出成本控制的精细化管理措施,BIM技术在不同工程控制过程中的关键作用和管理效果。

关键词：建筑模型信息化 BIM技术数据管理工程控制精细化

一种中药药效的多指标评价新方法——综合权重法

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中药新药与临床药理 2011年第5期22卷 569-572页

作者：马瑞娟苗明三河南中医学院郑州450008

目的建立一种多指标评价中药药效强弱的新方法。方法分析近年来医学上常用生物数学评价方法,借鉴其思路,结合中药药效特点,用数学公式建立一种新的评价中药药效的方法。结果目前常用生物数学评价方法各有优势和不足,提出的多指标评价中... 详细信息

目的建立一种多指标评价中药药效强弱的新方法。方法分析近年来医学上常用生物数学评价方法,借鉴其思路,结合中药药效特点,用数学公式建立一种新的评价中药药效的方法。结果目前常用生物数学评价方法各有优势和不足,提出的多指标评价中药药效的综合权重法,原理简单、应用方便、结果可信。结论综合权重法是一种适宜评价中药药效的新方法,突出了中药药效的特点。

关键词：多指标评价中药药效综合权重法

藏红花素减轻皮质神经元缺氧复氧损伤与抑制TLR4/NF-κB信号通路有关

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中国动脉硬化杂志 2023年第4期31卷 329-335,342页

作者：胡科李晓蕾麻瑞娟邯郸市中心医院神经内科河北省邯郸市056000

[目的]探讨藏红花素对皮质神经元缺氧复氧损伤的影响及其分子机制。[方法]分离并培养SD大鼠皮质神经元,设对照组、模型组、藏红花素(50 mg/L)组、藏红花素(50 mg/L)+脂多糖(TLR4激活剂,100μg/L)组。对照组常规培养,模型组建立缺氧4 h复... 详细信息

[目的]探讨藏红花素对皮质神经元缺氧复氧损伤的影响及其分子机制。[方法]分离并培养SD大鼠皮质神经元,设对照组、模型组、藏红花素(50 mg/L)组、藏红花素(50 mg/L)+脂多糖(TLR4激活剂,100μg/L)组。对照组常规培养,模型组建立缺氧4 h复氧24 h模型,藏红花素组和藏红花素+脂多糖组分别给药干预24 h后造模。通过CCK-8法、Annexin V-FITC/PI双染法检测神经元活力和凋亡率,ELISA法检测培养液中炎症因子水平,Western blot检测Toll样受体4(TLR4)、核因子κB p65(NF-κB p65)、p-NF-κB p65、NF-κB抑制蛋白α(IκBα)、p-IκBα、高迁移率族蛋白B1(HMGB1)、cleaved Caspase-3、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)表达。[结果]与对照组比较,模型组大鼠皮质神经元活力明显降低(P<0.05),凋亡率、培养液中TNF-α、IL-1β、IL-6水平、神经元TLR4、HMGB1、cleved Caspase-3表达量及p-NF-κB p65/NF-κB p65、p-IκBα/IκBα、Bax/Bcl-2比值均明显升高(P<0.05)。与模型组比较,藏红花素组皮质神经元活力升高94.97%(P<0.05),凋亡率降低65.80%(P<0.05),培养液中TNF-α、IL-1β、IL-6水平分别降低61.86%、78.34%、63.42%(P<0.05),神经元TLR4、HMGB1、cleaved Caspase-3表达量及p-NF-κB p65/NF-κB p65、p-IκBα/IκBα、Bax/Bcl-2比值分别降低73.43%、52.13%、81.52%、69.70%、60.55%、95.05%(P<0.05)。脂多糖明显逆转藏红花素对缺氧复氧损伤皮质神经元上述各指标的调控作用(P<0.05)。[结论]藏红花素可抑制缺氧复氧损伤皮质神经元凋亡和炎症反应,对缺氧复氧损伤皮质神经元起到保护作用,其机制可能与抑制TLR4/NF-κB信号通路有关。

关键词：藏红花素神经元缺氧复氧 TLR4/NF-κB通路炎症反应细胞凋亡

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固定化酶制备鳀鱼蒸煮液蛋白肽及其性能表征

食品与发酵工业 2020年第9期46卷 122-127页

作者：马瑞娟林煌华谢友坪陈剑锋福州大学海洋生物高值高质化利用技术创新服务平台福建福州350108 福州大学福建省海产品废弃物综合利用工程技术研究中心福建福州350108 福州大学福州市海产品高值化利用行业技术创新中心福建福州350108

以鳀鱼蒸煮液为原料,采用磁性交联酶聚集体技术制备的固定化酶酶解制备蛋白多肽,并分析水解肽的性能。在单因素试验基础上,运用中心组合设计法优化水解肽的制备工艺,并对酶解产物的氨基酸组成、分子质量分布、抗氧化性以及固定化酶的重... 详细信息

以鳀鱼蒸煮液为原料,采用磁性交联酶聚集体技术制备的固定化酶酶解制备蛋白多肽,并分析水解肽的性能。在单因素试验基础上,运用中心组合设计法优化水解肽的制备工艺,并对酶解产物的氨基酸组成、分子质量分布、抗氧化性以及固定化酶的重复利用性进行分析。优化得到的酶解工艺为:酶解时间45 min,酶解温度54.1℃,酶解pH 7.8,固定化碱性蛋白酶与中性蛋白酶比例为1.5∶1,在此条件下三氯乙酸氮溶解指数(trichloracetic acid-nitrogen solubility index, TCA-NSI)可达77.27%。酶解液的必需氨基酸指数大于0.95,分子质量低于1 500 Da的小肽占82.52%,且具有较强的抗氧化性。此外,固定化酶循环利用5次时TCA-NSI值仍可保持首次使用时的48%。优化后的工艺具有多肽得率高,酶解液营养价值高且抗氧化性强,固定化酶可重复利用等优点,可为低值鱼蒸煮液的高值化利用提供理论参考。

关键词：鳀鱼蒸煮液固定化酶蛋白多肽抗氧化