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Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

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Biomedical and Environmental Sciences 2024年第3期37卷 294-302页

作者： ZHANG Ya Li SU Wen Zhe WANG Rui Chen LI Yan ZHANG Jun feng LIU Sheng Hui HU Dan He XU Chong Xiao YIN Jia Yu YIN Qi Kai HE Ying LI Fan FU Shi Hong NIE Kai LIANG Guo Dong TAO Yong XU Song Tao ma chao feng WANG Huan Yu Shaanxi University of Chinese Medicine Xianyang 712000ShaanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Guangzhou Center for Disease Control and Prevention Guangzhou 510000GuangdongChina Shandong Center for Disease Control and Prevention Jinan 250000ShandongChina Department of Ophthalmology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Xi'an Center for Disease Control and Prevention Xi’an 710054ShaanxiChina

Objective Viral encephalitis is an infectious disease severely affecting human *** is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other *** laboratory diagnosis of viral encephalitis is a worldwide ***,high-throughput sequencing technology has provided new tools for diagnosing central nervous system ***,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon *** We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant *** detection ability of the primers was verified by software simulation and the detection of known positive *** sequencing was used to validate the samples,and consistency was compared with Sanger *** The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted *** sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger *** Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral *** is also a useful tool for the high-volume screening of clinical samples.

关键词： Viral encephalitis Amplicon sequencing High-throughput sequencing Multipathogen detection

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乡村数智化治理的新质生产力逻辑、机制及进路

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深圳大学学报（人文社会科学版） 2024年第4期41卷 97-105页

作者：肖龙马超峰延安大学政法与公共管理学院陕西延安716000 南京林业大学人文社会科学学院江苏南京210037

乡村数智化治理是我国全面推进乡村振兴战略中面临的重大理论和实践问题。乡村社会是国家治理的“神经末梢”,关乎党在基层的执政根基,也是实现国家治理体系与治理能力现代化的薄弱环节。乡村数智化治理以大数据为基础,结合人工智能、... 详细信息

乡村数智化治理是我国全面推进乡村振兴战略中面临的重大理论和实践问题。乡村社会是国家治理的“神经末梢”,关乎党在基层的执政根基,也是实现国家治理体系与治理能力现代化的薄弱环节。乡村数智化治理以大数据为基础,结合人工智能、数字孪生、元宇宙、区块链等新质生产力技术,实现乡村社会治理效能的整体提升。首先,数智技术嵌入农业产业催生新质农业生产力,实现新质农业劳动者、新质农业生产资料、新质农业生产对象的优化组合,将有力地推动农业产业的高质量发展,实现农村生产关系的变革。其次,农业新质生产力通过乡村数字经济发展、乡村数字社会建设、乡村数字政务建设等驱动机制实现乡村数智化治理转型。通过运用数字孪生技术,实现乡村社会的数字画像;通过数字技术转化,实现乡村社会治理的数字塑型;通过优化数智化场景应用,实现乡村社会治理的数智铸魂。最后,农业新质生产力驱动的乡村数智化治理转型,在解决乡村社会治理系列难题的同时,还需要通过人工智能与群众智慧的“双智融合”、防范数智化的技术风险、促使“科技向善”、因地制宜“梯度嵌入”等进路,推动乡村数智化治理的系统性优化。

关键词：乡村数智化治理新质生产力农村生产关系理论逻辑驱动机制实践进路

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河南省部分地区奶牛场病毒性腹泻血清流行病学调查

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动物医学进展 2024年第7期45卷 135-139页

作者：叶丛华王满红马超锋赵文凤河南省固始县动物疫病预防控制中心河南信阳465200 河南省西平县畜牧综合服务中心河南驻马店463900 河南省信阳市畜牧兽医技术服务中心河南信阳464000 信阳航空职业学院公共教学部河南信阳464000

为掌握河南省奶牛场病毒性腹泻流行情况,于2020年9月至2023年2月,采用ELISA对采自河南省18个地区30个奶牛场的853份血清样品中病毒性腹泻的抗体进行了检测分析。结果显示,病毒性腹泻病毒抗体阳性场27个,场阳性率高达90%;阳性样品475份,... 详细信息

为掌握河南省奶牛场病毒性腹泻流行情况,于2020年9月至2023年2月,采用ELISA对采自河南省18个地区30个奶牛场的853份血清样品中病毒性腹泻的抗体进行了检测分析。结果显示,病毒性腹泻病毒抗体阳性场27个,场阳性率高达90%;阳性样品475份,个体阳性率为55.7%;不同生长阶段牛群阳性率差异极显著(P<0.01),其中育成牛(14~24月龄)阳性率最高(75%),犊牛(3月龄以下)阳性率最低(20.5%);不同规模奶牛场阳性率差异极显著(P<0.01),其中大型场(500头以上)阳性率最高(66.8%),而小型场(200头以下)的阳性率最低(34.1%)。研究结果为奶牛场病毒性腹泻防控提供了重要参考依据。

关键词：奶牛病毒性腹泻酶联免疫吸附试验阳性率

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牛病毒性腹泻抗体制剂的制备及其应用研究

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中国牛业科学 2024年第1期50卷 19-23页

作者：项朝荣郑虎马超锋徐华济源市动物疫病预防控制中心河南济源459000 罗山县畜牧兽医技术服务中心河南罗山464200 信阳市畜牧兽医技术服务中心河南信阳464000

抗体制剂是牛病毒性腹泻最为有效的临床治疗药剂,但现有制剂易在瘤胃中降解而药效不佳。本研究利用抗体包被技术开发了一种能有效避免瘤胃降解的牛病毒性腹泻抗体制剂的制备方法,对抗体制剂筛选的6个组方体系进行稳定性、安全性和药效... 详细信息

抗体制剂是牛病毒性腹泻最为有效的临床治疗药剂,但现有制剂易在瘤胃中降解而药效不佳。本研究利用抗体包被技术开发了一种能有效避免瘤胃降解的牛病毒性腹泻抗体制剂的制备方法,对抗体制剂筛选的6个组方体系进行稳定性、安全性和药效综合考察,表明体系1(抗体5.0g、卵磷脂20.0g、聚乙二醇80015g、β-谷甾醇1.0g、鞣云实素3.0g、吐温-2015.0g、去离子水41.0g)为最优组方,临床疗效显著高于普通抗体制剂。该制剂具有稳定性强和安全性高的特点,能有效解除瘤胃液对口服抗体的影响,促进其在肠道中的高效吸收和利用,对临床防控牛病毒性腹泻有着重要意义。

关键词：牛牛病毒性腹泻抗体制剂

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How can technology and efficiency alleviate the dilemma of economic growth and carbon emissions in China's industrial economy? A metafrontier decoupling decomposition analysis

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Petroleum Science 2024年第2期21卷 1415-1428页

作者： Miao Wang chao feng School of Business Zhengzhou UniversityZhengzhou450001HenanChina School of Economics and Business Administration Chongqing UniversityChongqing400030China

This paper attempts to explore the decoupling relationship and its drivers between industrial economic increase and energy-related CO_(2) emissions(ICE). Firstly, the decoupling relationship was evaluated by Tapio index. Then, based on the DEA meta-frontier theory framework which taking into account the regional and industrial heterogeneity and index decomposition method, the driving factors of decoupling process were explored mainly from the view of technology and efficiency. The results show that during2000-2019, weak decoupling was the primary state. Investment scale expansion was the largest reason hindering decoupling process of industrial increase from ICE. Both energy saving and production technology achieved significant progress, which facilitated the decoupling process. Simultaneously, the energy technology gap and production technology gap among regions have been narrowed, and played a role in promoting decoupling process. On the contrary, both scale economy efficiency and pure technical efficiency have inhibiting effects on decoupling process. The former indicates that the scale economy of China's industry was not conducive to improve energy efficiency and production efficiency, while the latter indicates that resource misallocation problem may exist in both energy market and product market.

关键词： China's industrial sector Decoupling process Meta-frontier DEA Index decomposition method Driving factors

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GNAS mutations suppress cell invasion by activating MEG3 in growth hormone–secreting pituitary adenoma

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Oncology Research 2024年第6期32卷 1079-1091页

作者： chao TANG CHUNYU ZHONG JUNHAO ZHU feng YUAN JIN YANG YONG XU CHIYUAN ma Department of Neurosurgery Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjing210002China Department of Neurosurgery Children’s Hospital of Nanjing Medical UniversityNanjing210019China Affiliated Eye Hospital Nanjing Medical UniversityNanjing210029China Department of Neurosurgery Jinling HospitalSouthern Medical UniversityNanjing210002China Department of Neurosurgery Jinling HospitalNanjing Medical UniversityNanjing210002China

Approximately 30%–40%of growth hormone–secreting pituitary adenomas(GHPAs)harbor somatic activating mutations in GNAS(αsubunit of stimulatory G protein).Mutations in GNAS are associated with clinical features of smaller and less invasive ***,the role of GNAS mutations in the invasiveness of GHPAs is *** mutations were detected in GHPAs using a standard polymerase chain reaction(PCR)sequencing *** expression of mutation-associated maternally expressed gene 3(MEG3)was evaluated with ***3 was manipulated in GH3 cells using a lentiviral expression *** invasion ability was measured using a Transwell assay,and epithelial–mesenchymal transition(EMT)-associated proteins were quantified by immunofluorescence and western ***,a tumor cell xenograft mouse model was used to verify the effect of MEG3 on tumor growth and *** invasiveness of GHPAs was significantly decreased in mice with mutated GNAS compared with that in mice with wild-type ***,the invasiveness of mutant GNASexpressing GH3 cells ***3 is uniquely expressed at high levels in GHPAs harboring mutated ***,MEG3 upregulation inhibited tumor cell invasion,and conversely,MEG3 downregulation increased tumor cell ***,GNAS mutations inhibit EMT in ***3 in mutated GNAS cells prevented cell invasion through the inactivation of the Wnt/β-catenin signaling pathway,which was further validated in *** data suggest that GNAS mutations may suppress cell invasion in GHPAs by regulating EMT through the activation of the MEG3/Wnt/β-catenin signaling pathway.

关键词： GHPAs GNAS mutation MEG3 β-catenin EMT

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Effects of Lactobacillus paracasei N1115 on gut microbial imbalance and liver function in patients with hepatitis B-related cirrhosis

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World Journal of Gastroenterology 2024年第11期30卷 1556-1571页

作者： Yan-chao Hu Xiang-Chun Ding Hui-Juan Liu Wan-Long ma Xue-Yan feng Li-Na ma Department of Infectious Disease General Hospital of Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Infectious Disease Clinical Research Center of Ningxia Yinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Hepatitis B cirrhosis(HBC)is a chronic disease characterized by irreversible diffuse liver damage and aggravated by intestinal microbial imbalance and metabolic *** the relationship between certain single probiotics and HBC has been explored,the impact of the complex ready-to-eat Lactobacillus paracasei N1115(LP N1115)supplement on patients with HBC has not been *** To compare the changes in the microbiota,inflammatory factor levels,and liver function before and after probiotic treatment in HBC *** This study included 160 HBC patients diagnosed at the General Hospital of Ningxia Medical University between October 2018 and December *** were randomly divided into an intervention group that received LP N1115 supplementation and routine treatment and a control group that received routine treatment *** samples were collected at the onset and conclusion of the 12-wk intervention *** structure of the intestinal microbiota and the levels of serological indicators,such as liver function and inflammatory factors,were *** Following LP N1115 intervention,the intestinal microbial diversity significantly increased in the intervention group(Pmatory factors(Pmatory factor levels.

关键词： Hepatitis B cirrhosis N1115 ready-to-eat lactobacillus Inflammation Liver function Lachnospiraceae incertae sedis Probiotic

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Clinical application of COVID-19 vaccine in liver transplant recipients

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Hepatobiliary & Pancreatic Diseases International 2024年第4期23卷 339-343页

作者： feng-chao Liu man Xie Wei Rao Division of Hepatology Liver Disease CenterThe Affiliated Hospital of Qingdao UniversityQingdao 266000China Organ Transplantation Center The Affiliated Hospital of Qingdao UniversityQingdao 266000China Institute of Organ Donation and Transplantation of Qingdao University Qingdao 266000China Department of Gastroenterology The Affiliated Hospital of Qingdao UniversityQingdao 266000China

Background:Solid organ transplant(SOT)activities,such as liver transplant,have been greatly influenced by the pandemic of coronavirus disease 2019(COVID-19),a disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Immunosuppressed individuals of liver transplant recipients(LTRs)tend to have a high risk of COVID-19 infection and related ***,COVID-19 vaccination has been recommended to be administered as early as possible in *** sources:The keywords“liver transplant”,“SARS-CoV-2”,and“vaccine”were used to retrieve articles published in ***:The antibody response following the 1st and 2nd doses of vaccination was disappointingly low,and the immune responses among LTRs remarkably improved after the 3rd or 4th dose of *** the 3rd or 4th dose of COVID-19 vaccine increased the antibody titer,a proportion of patients remained ***,recent studies showed that SARS-CoV-2 vaccine could trigger adverse events in LTRs,including allograft rejection and liver ***:This review provides the recently reported data on the antibody response of LTRs following various doses of vaccine,risk factors for poor serological response and adverse events after vaccination.

关键词： Liver transplant COVID-19 SARS-CoV-2 Vaccine Antibody response

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CToMP: a cycle-task-oriented memory protection scheme for unmanned systems

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Science China(Information Sciences) 2024年第6期67卷 399-417页

作者： Chengyan ma Ning XI Di LU Yebo feng Jianfeng ma School of Cyber Engineering Xidian University School of Computer Science and Technology Xidian University Department of Computer and Information Science University of Oregon

Memory corruption attacks(MCAs) refer to malicious behaviors of system intruders that modify the contents of a memory location to disrupt the normal operation of computing systems, causing leakage of sensitive data or perturbations to ongoing processes. Unlike general-purpose systems, unmanned systems cannot deploy complete security protection schemes, due to their limitations in size, cost and *** in unmanned systems are particularly difficult to defend against. Furthermore, MCAs have diverse and unpredictable attack interfaces in unmanned systems, severely impacting digital and physical sectors. In this paper, we first generalize, model and taxonomize MCAs found in unmanned systems currently, laying the foundation for designing a portable and general defense approach. According to different attack mechanisms,we found that MCAs are mainly categorized into two types — return2libc and return2shellcode. To tackle return2libc attacks, we model the erratic operation of unmanned systems with cycles and then propose a cycle-task-oriented memory protection(CToMP) approach to protect control flows from tampering. To defend against return2shellcode attacks, we introduce a secure process stack with a randomized memory address by leveraging the memory pool to prevent Shellcode from being executed. Moreover, we discuss the mechanism by which CTo MP resists the return-oriented programming(ROP) attack, a novel variant of return2libc attacks. Finally, we implement CTo MP on CUAV V5+ with Ardupilot and Crazyflie. The evaluation and security analysis results demonstrate that the proposed approach CTo MP is resilient to various MCAs in unmanned systems with low footprints and system overhead.

关键词： unmanned system memory corruption attack memory protection system security randomized memory address

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Immune checkpoint inhibition mediated with liposomal nanomedicine for cancer therapy

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Military Medical Research 2024年第1期11卷 98-114页

作者： Guang-Long ma Wei-feng Lin Faculty of Medicine Centre for Cancer ImmunologyUniversity of SouthamptonSouthampton SO166YDUK Department of Molecular Chemistry and materials Science Weizmann Institute of Science76100 RehovotIsrael Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China

Immune checkpoint blockade(ICB)therapy for cancer has achieved great success both in clinical results and on the *** the same time,success drives more attention from scientists to improve ***,only a small portion of patients are responsive to this therapy,and it comes with a unique spectrum of side effects termed immunerelated adverse events(irAEs).The use of nanotechnology could improve ICBs’delivery to the tumor,assist them in penetrating deeper into tumor tissues and alleviate their *** nanomedicine has been investigated and used for decades,and is well-recognized as the most successful nano-drug delivery *** successful combination of ICB with liposomal nanomedicine could help improve the efficacy of ICB *** this review,we highlighted recent studies using liposomal nanomedicine(including new emerging exosomes and their inspired nanovesicles)in associating ICB therapy.

关键词： Liposome Exosome Immune checkpoint blockade(ICB)

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