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Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and moths of Bombyx mori

Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das Neves Saez Roxelle Ethienne Ferreira munhoz Naiara Climas Pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose meire costa Brancalhao maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains m18-2, m12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for m11-A;33% for AS3, B106, m8 and m11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

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Light Reflection by Cuticles of Chrysina Jewel Scarabs: Optical measurements, morphology Characterization, and Theoretical modeling

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Optics and Photonics Journal 2016年第7期6卷 146-163页

作者： W. E. Vargas m. Hernández-Jiménez E. Libby D. E. Azofeifa C. Barboza Á. Solis Centro de Investigación en Ciencia e Ingeniería de materiales & Escuela de Física Universidad de Costa Rica San José Costa Rica Academia Nacional de Ciencias de costa Rica San José Costa Rica Centro de Investigación en Ciencia e Ingeniería de materiales and Escuela de Química Universidad de Costa Rica San José Costa Rica Centro de Investigación en Estructuras microscópicas Universidad de Costa Rica San José Costa Rica museo Nacional de costa Rica San José Costa Rica

Cuticles of some Chrysina scarabs are characterized by flat, graded, and twisted structures of nanosized chitin fibrils. As inferred from SEm images, each species has its own spatial period or pitch P which is dependent on the depth z through the cuticle. From Berreman’s formalism, taking into account the corresponding P(z) dependence, we evaluate reflection spectra of C. aurigans and C. chrysargyrea scarabs. The spectra display the main spectral features observed in the measured ones when small sections of the cuticles are illuminated with non-polarized light, for wavelengths between 300 and 1100 nm. By considering these twisted structures as 1D photonic crystals, an approach is developed to show how the broad band characterizing the reflection spectra arises from a narrow intrinsic photonic band width, whose spectral position moves through visible and near infrared wavelengths. The role of the epicuticle that covers the twisted structures is analyzed in terms of a waxy layer acting as an anti-reflecting coating that also shows low levels of light scattering.

关键词： Structural Color Structural Chirality Natural Broad Band Reflector Circular Polarization Jewel Scarab Chitin Refractive Index Uric Acid Refractive Index Left-Handed Twisted Structure Bouligand-Type Structure

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Association between Infrared Thermography, Blood Count and Creatine Kinase in the Evaluation of the Welfare of Vaquejada Horses

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Open Journal of Veterinary medicine 2023年第6期13卷 53-67页

作者： Carolina Jones Ferreira Lima da Silva Keity Laiane Gomes Trindade Raissa Karolliny Salgueiro Cruz César Fabiano Vilela Clarisse Simões Coelho José Dantas Ribeiro Filho Helena Emília Cavalcanti da costa Cordeiro manso Hélio Cordeiro manso Filho Núcleo de Pesquisa Equina Universidade Federal Rural de Pernambuco Recife Brazil Faculdade de medicina Veterinária Centro Universitário Cesmac Maceió Brazil médico Veterinário Privado Americana Brazil Faculdade de medicina Veterinária Universidade Lusófona de Humanidades e Tecnologias Lisboa Portugal Departamento de medicina Veterinária Universidade Federal de Vi&#231osa Vi&#231osa Brazil

The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, indicating the great physical effort during competitions and with that could impact welfare. To test the hypothesis that vaquejada horses can be evaluated for the quality of welfare through a combination of non-invasive and invasive methods, an experiment was developed that aimed to assess the quality of welfare through thermography and blood biomarkers before and after vaquejada races. Ten vaquejada horses, which were in regular competition, were submitted to the vaquejadas racing test were used. Thermography was performed in 14 regions of interest (head, neck and thorax) in the following phases: pre-race and +1, +4 and +24 hours of the races. Blood samples were collected in pre-race, immediately after and +1, +4 and +24 hours of the races. Results were submitted to ANOVA and Tukey’s test, with p set at 5%. Thermography showed that local temperatures before the race and +24 hours after the races were similar (p > 0.05), as well as between +1 and +4 hours after the races (p > 0.05), which showed higher surface temperatures than the first group (pre-race and +24 hours) (p 0.05) after the exercises. In conclusion, the vaquejada horses evaluated by infrared thermography and blood analyses maintained the quality of well-being, with the physiological elevation of several parameters after the races and recovery in less than 24 hours after the races. Thermographic images were also able to help in the analysis of the regions of interest that have contact with harnesses, demonstrating that the use of adequate and correct equipment does not compromise animal welfare.

关键词： Exercise Equine CK Hematology Equestrian Sports Well-Being

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与对克罗恩病复发的预测相比,钙卫蛋白是溃疡性结肠炎复发的较强预测指标

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世界核心医学期刊文摘（胃肠病学分册） 2005年第7期1卷 33-33页

作者： costa F. mumolo m.G. Ceccarelli L. 陈云茹 Dipartimento di medicina Interna Universitá di PisaOspedale SChiara Via Roma 67- 56122 Pisa Italy Dr.

Background and aims: The clinical course of inflammatory bowel disease is cha racterised by a succession of relapses and remissions. The aim of our study was to assess whether the predictive value of faecal calprotectin, a non- invasive marker of intestinal inflammation, for clinical relapse is different in ulcera tive colitis (UC) and Crohn’ s disease (CD). methods: Seventy nine consecutive patients with a diagnosis of clinically quiescent inflammatory bowel disease (38 CD and 41 UC) were followed for 12 months, undergoing regular clinical evaluati ons and blood tests. A single stool sample was collected at the beginning of the study from each patient and the calprotectin concentration was assessed by a co mmercially available enzyme linked immunoassay. Results: In CD, median calprotec tin values were 220.1 μ g/g (95% confidence interval (CI) 21.7- 418.5) in th ose patients who relapsed during follow up, and 220.5 μ g/g (95% CI 53- 388) in non- relapsing patients (p = 0.395). In UC, median calprotectin values were 220.6 μ g/g (95% CI 86- 355.2) and 67 μ g/g (95% CI 15- 119) in relapsi ng and non- relapsing patients, respectively (pmultivariate Cox (proportional hazard) regression model, after adjustment for possible confoundin g variables, showed a twofold and 14- fold increase in the relapse risk, respec tively, in those patients with CD and UC in clinical remission who had a faecal calprotectin concentration higher than 150 μ g/g. Conclusions: Faecal calprotec tin proved to be an even stronger predictor of clinical relapse in UC than in CD , which makes the test a promising non- invasive tool for monitoring and optimi zing therapy.

关键词：克罗恩病溃疡性结肠炎炎症性肠病粪便样本临床缓解期预测价值非侵入性检查临床病程酶联免疫法血液检查

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难以确诊的实体物:出血性腹部阴囊水囊肿

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世界核心医学期刊文摘（儿科学分册） 2006年第2期2卷 40-40页

作者： Estevo-costa J. morgado H. Soares-Oliveira m. 李丹 Servico Cirurgica Pediat. Faculdade Medicina Porto Hospital So Joo 4200-319 Porto Portugal

Abdominoscrotal hydrocele (ASH) is a rare condition in childhood. It presents an intraabdominal portion with homogeneous content that is usually excised through a groin approach. A pale child with a recurrent scrotal hydrocele and a huge solidcystic retroperitoneal mass is reported. Because the diagnostic workup has not ruled out malignancy, a laparotomy was performed; however, ultimate diagnosis was hemorrhagic ASH. The clinical features and surgical management of ASH are addressed. This entity should be considered in front of tense or recurrent hydroceles or coexistent abdominal mass even though with heterogeneous content.

关键词：水囊肿实体性出血性阴囊腹部剖腹探查手术腹膜后包块确诊儿童时期面色苍白

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哥斯达黎加地震的结构震害

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世界地震工程 1993年第2期9卷 47-50,30页

作者： m. J. Nigel Priestley 董文芝

一、概述 1991年4月22日,在哥斯达黎加的人口较稀地区发生了震级为7.4级的地震。尽管地貌效应显著,震中山区产生了大面积滑波,东海岸隆起达1.5米,但结构震害比较说来要轻些。不过,在利蒙(Limon)港和邻近地区,许多仓库和钢筋混凝土建筑... 详细信息

一、概述 1991年4月22日,在哥斯达黎加的人口较稀地区发生了震级为7.4级的地震。尽管地貌效应显著,震中山区产生了大面积滑波,东海岸隆起达1.5米,但结构震害比较说来要轻些。不过,在利蒙(Limon)港和邻近地区,许多仓库和钢筋混凝土建筑倒塌或严重破坏,大量高木桩支承的屋屋毁坏。最值得注意的是主要由于地基液化或堤岸软土层的过量运动,使许多现代化公路桥遭到毁坏。

关键词：地震震级结构震害

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mean and Interannual Variability of maize and Soybean in Brazil under Global Warming Conditions

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American Journal of Climate Change 2013年第4期2卷 237-253页

作者： Flávio Justino Evandro Chaves Oliveira Rafael de Avila Rodrigues Paulo Henrique Lopes Goncalves Paulo Jorge Oliveira Ponte Souza Frode Stordal José marengo Thieres G.da Silva Rafael Coll Delgado Douglas da Silva Lindemann Luiz Claudio costa Departamento de Engenharia Agrícola Universidade Federal de ViçosaVicosaBrazil Universidade Federal Rural da Amazônia Instituto Sócio-Ambiental e dos Recursos HídricosBelémBrazil Department of Geosciences University of OsloOsloNorway Instituto Nacional de Pesquisas Espaciais Cachoeira PaulistaBrazil Universidade Federal Rural de Pernambuco Unidade Academica de Serra TalhadaSerra TalhadaBrazil Universidade Federal Rural do Rio de Janeiro Instituto de Florestas/Departamento de Ciencias AmbientaisSeropédicaBrazil

Brazil is responsible for 27% of the world production of soybeans and 7% of maize. mato Grosso and Para states in Brazil are among the largest producer. The viability to the cultivation of maize (Zea mays) and soybeans (Glycine max), for future climate scenarios (2070-2100, GHG) is evaluated based on crop modeling (DSSAT) forced by observational data and regional climate simulations (HadRm3). The results demonstrated that a substantial reduction in the yield in particular for maize may be expected for the end of the 21st century. Distinct results are found for soybeans. By applying the A2 climate changes scenario, soybean yield rises by up top 60% assuming optimum soil treatment and no water stress. However, by analyzing the inter-annual variability of crop yields for both maize and soybean, could be demonstrated larger year-to-year fluctuations under greenhouse warming conditions as compared to current conditions, leading to very low productivity by the end of the 21st century. Therefore, these Brazilian states do not appear to be economically suitable for a future cultivation of maize and soybeans. Improved adaptation measures and soil management may however partially alleviate the negative climate change effect.

关键词： Climate Changes Soybeans maize

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氮素改变辣椒初级代谢和薄壁组织厚度调控比叶面积研究

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辣椒杂志 2021年第3期19卷 36-48页

作者： Lucas de ávila Silva Rebeca P.Omena-Garcia Jorge A.Condori-Apfata Paulo mafra de Almeida costa Natália machado Silva Fábio m.Da matta Agustin Zsogon Wagner L.Araújo Edgard A.de Toledo Picoli Ronan Sulpice Adriano Nunes-Nesi 沈凌峰吉雪花(译) 不详石河子大学农学院乌鲁木齐832003

比叶面积(SLA)是影响植物光截获和光利用效率的关键性状,常常影响植物的生长和产量。SLA是解释不同植物在不同环境下生长差异的一个关键性状。光照和氮素供应都是决定SLA的重要因素。为了更好地了解辐照度和氮素对辣椒(Capsicum chinens... 详细信息

比叶面积(SLA)是影响植物光截获和光利用效率的关键性状,常常影响植物的生长和产量。SLA是解释不同植物在不同环境下生长差异的一个关键性状。光照和氮素供应都是决定SLA的重要因素。为了更好地了解辐照度和氮素对辣椒(Capsicum chinense)SLA的影响,研究对两个中国辣椒商品种(Biquinho和Habanero)的初级代谢产物和形态特征进行了分析。两种基因型辣椒的SLA随着遮阴而增加,但随氮供应量的增加而下降;在氮素供应由缺乏到充足的范围内Habanero品种的SLA表现稳定。相关分析表明,高氮供应导致SLA降低是因为氨基酸、蛋白质和淀粉含量的调节,从而影响叶片密度。此外,在中度缺氮到充足供氮范围内,两种基因型辣椒的SLA表现出响应差异,Biquinho和Habanero分别在栅栏和海绵状薄壁组织上表现出差异。总之,结果表明,SLA对氮供应的反应是由某些代谢物含量和依赖于基因型的影响叶片厚度及密度的薄壁组织变化平衡调节的。

关键词：辣椒叶片形态和解剖结构组织密度遮阴

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波尔图Kinematix办公室

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现代装饰 2015年第6期 112-115页

作者： Diogo Aguiar Teresa Otto Joo Jesus Tània costa Coll lvaro Vila Iturbe Dinis Sottomayor 姜敏华 LIKE建筑事务所

项目地址葡萄牙项目面积180m^2竣工时间2014.05设计单位LIKE建筑事务所本案是LIKE建筑事务所的设计团队为一家专业致力于医疗保健技术和健身产品的IT公司Kinematix打造的研发总部。项目坐落于葡萄牙第二大城市——波尔图一栋具有象征意... 详细信息

项目地址葡萄牙项目面积180m^2竣工时间2014.05设计单位LIKE建筑事务所本案是LIKE建筑事务所的设计团队为一家专业致力于医疗保健技术和健身产品的IT公司Kinematix打造的研发总部。项目坐落于葡萄牙第二大城市——波尔图一栋具有象征意义的六十年代历史建筑内,其前身为卢米埃尔电影院。该建筑上层现存的空间安装有排列整齐的窗户,覆盖建筑物的整个立面,极具韵律感。

关键词：波尔建筑事务所 Kinematix 项目地址自然光线设计单位历史建筑韵律感象征意义项目面积

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对西罗莫司洗脱支架治疗患者进行4年血管造影和血管内超声随访

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世界核心医学期刊文摘（心脏病学分册） 2005年第10期1卷 36-36页

作者： Sousa J. E. costa m. A. Abizaid A. 高登峰 Institute Dante Pazzanese of Cardiology Avenida Dr Dante Pazzanese 500 04012180 S o Paulo Brazil

Despite the proven superiority of sirolimus-eluting stents(SESs) compared with bare stents in the first year after implantation, long-term outcomes of patients treated with these novel devices remain unknown. Our goal was to evaluate the clinical, angiographic, and intravascular ultrasound(IVUS) outcomes of patients treated with SESs 4 years after implantation. methods and Results-The study included 30 patients treated with sirolimus-eluting Bx Velocity stenting(slow release[SR; n=15] and fast release[FR; n=15]). Twenty-six patients underwent 4- year angiographic and IVUS follow-up and had matched assessments at all time points(index and 4- , 12- , 24- , and 48- month follow-up). One death occurred during the study period in a patient with a patent SES. There were no target-vessel revascularizations or thromboses between 2- and 4- year follow-up examinations. There was no stent thrombosis, target-lesion revascularization, death, or myocardial infarction in the SR group up to 4 years. Cumulative event-free survival rate was 87% for the total population(80% in the FR group and 93% in the SR group). In-stent late loss was slightly greater in the FR group(0.41± 0.49 mm) than the SR group(0.09± 0.23)after 4 years. One patient in the FR group had a 52% in-stent restenosis lesion. Percent neointimal hyperplasia volume, as detected by IVUS, remained minimal after 4 years(FR=9.1% and SR=5.7% ). Conclusions-This study confirms the longevity of the optimal outcomes observed in patients treated with sirolimus-eluting Bx Velocity stents 4 years after implantation. In-stent lumen dimensions remained essentially unchanged at 4-year follow-up, particularly in the population treated with the currently available SES(SR formulation).

关键词：血管内超声血管造影靶血管血运重建西罗莫司再狭窄无事件生存率血栓形成内膜增生体积百分比最小限度

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