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Culture techniques and growth characteristics of Dinophysis acuminata and its prey

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Chinese Journal of Oceanology and Limnology 2010年第6期28卷 1230-1239页

作者：佟蒙蒙周启星 KULIS m.David 江天久齐雨藻 ANDERSON m.Donald Key Laboratory of Pollution Processes and Environmental Criteria(ministry of Education) College of Environmental Science and EngineeringNankai University Woods Hole Oceanographic Institution Research Center for Red Tide and Aquatic Environments Jinan University

The dinoflagellate Dinophysis acurninata Claparede ＆ Lachrnann is a toxic alga that causes diarrhetic shellfish poisoning. No Dinophysis species were maintained in culture for a long period of time until 2006 when Park successfully established D. acuminata in culture using a three-step feeding protocol in which the cryptophyte, Geminigera cryophila, is fed to myrionecta rubra （=mesodinium rubrum）, a ciliate that is in turn fed to D. acuminata. In this paper, we present the details of culturing D. acuminata from the Northeastern United States. The protocols described herein can be adopted for laboratory studies of this species. The effects of temperature on the growth and ingestion rates of D. acuminata were also examined. The results show that D. acuminata growth rate was 0.23/d at 10℃ and 0.11/d at 4℃when fed m. rubra prey. The maximum prey ingestion rate was 2.80 Dinophysis cell/d at 10℃, although the rate decreased slightly at 4℃. In overall, temperature showed a greater influence on growth rate of D. acuminata than on the ingestion rate under the study conditions, and the quantity of available food was also an important regulator to D. acurninata growth.

关键词： Dinophysis acuminata myrionecta rubra Geminigera cryophila growth rate ingestion rate

Short-term effect of supplemental yeast extract without or with feed enzymes on growth performance, immune status and gut structure of weaned pigs challenged with Escherichia coli lipopolysaccharide

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Journal of Animal Science and Biotechnology 2017年第2期8卷 378-390页

作者： Samuel m.Waititu Fugui Yin Rob Patterson Juan C.Rodriguez-Lecompte Charles m.Nyachoti Department of Animal Science University of Manitoba201-12 Dafoe RoadWinnipegM6 R3T 2N2Canada Canadian Bio-systems Inc. CalgaryAB T2C OJTCanada Atlantic Veterinary College University of Prince Edward IslandCharlottetownPE C1A 4P3Canada

Background： This study investigated the response of piglets receiving a yeast extract without or with a multi-enzyme mixture compared with an antimicrobial growth promoter（AGP） on performance, immune status and gut structure after an E. coli lipopolysaccharide（LPS） challenge. Thirty-six pigs were allotted to six treatments including： a non-challenged control（NCC）; LPS-challenged control（CC）; CC ＋ AGP; CC ＋ yeast extract; CC ＋ enzymes; and CC ＋ enzymes ＋ yeast extract. On d 7, pigs were bled and thereafter injected with LPS or sterile saline. Blood samples were collected at 6, 48, and 96 h post-challenge. After 96 h post-challenge, pigs were euthanized to obtain duodenal, jejunal and ileal ***： Overall（d 1 to 11）, compared with CC pigs, AGP attenuated the LPS-induced reduction in ADG（P = 0.004）,ADFI（P = 0.03） and gain/feed ratio（P = 0.01）. At 6 h post-challenge, AGP pigs had lower plasma urea N（PUN; P = 0.02）and serum TNF-α concentration（P = 0.07）, and higher platelet count（P = 0.04） and serum IL-10 concentration（P = 0.02） than CC pigs. At 48 h post-challenge, AGP pigs had lower PUN（P = 0.02） than CC pigs, whereas enzymes ＋ yeast extract interacted non-additively（P = 0.001） to reduce PUN. At 96 h post-challenge, AGP pigs had lower PUN（P = 0.02）and higher duodenal（P = 0.03）, jejunal（P = 0.01） and ileal（P = 0.07） villus height than CC pigs. In addition, enzymes ＋yeast extract interacted additively and non-additively to reduce ileal IFN-γ（P 0.10） were observed between AGP and enzymes ＋ yeast extract pigs on other measured parameters except for the downregulation of ileal IFN-γ（P ＆lt; 0.0001） and TNF-α（P = 0.003） in enzymes ＋ yeast extract pigs at 96 h ***： The LPS challenged piglets receiving enzymes ＋ yeast extract showed beneficial responses in gut structure and immunity commensurate with those receiving antibiotics, though the latter had better overall growth performance.

关键词： Antimicrobial growth promoter Feed enzymes Growth performance Immunity Nucleotides Piglets

Flash Lamps as Ignition and Initiation Sources of the VS-2 Pyrotechnic Composition

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含能材料 2022年第4期30卷 370-378页

作者： Sergey I.Gerasimov mikhail A.Ilyushin Sergey m.Putis Sergey A.Dushenok Irina V.Shugalei Andrey m.Sudarikov Russian Federal Nuclear Center‑All‑Russian Research Institute of Experimental Physics SarovRussia Sarov Institute of Physics and Technology is a Branch of the Scientific Research Nuclear University mEPhI SarovRussia Nizhny Novgorod State Technical University Named after R.E.Alekseev Nizhny NovgorodRussia RAS Institute for Studying Problems in mechanical Engineering ‑is a Branch of the RAS Applied Physics InstituteNizhny NovgorodRussia "Technologist"Special Design and Technological Bureau St.PetersburgRussia Saint‑Petersburg State Institute of Technology(Technical University) St.PetersburgRussia Russian State Hydro‑meteorological University St.PetersburgRussia

In the present work it is found that the pyrotechnic composition VS⁃2 can be initiated with flash lamps IFC⁃500 and ***⁃2 pyrotechnic composition contains 90%of mercury(Ⅱ)5⁃hydrazinotetrazolate perchlorate and 10%of optically trans⁃parent copolymer of 2⁃methyl⁃5⁃vinyltetrazole and methacrylic acid(PVmT).We have found that the flash lamps make it possi⁃ble to initiate combustion of VS⁃2 composition with its transition to detonation both in cylindrical charges placed in brass caps of 5 mm diameter and 2 mm high,and film charges with 10 mm×80 mm in size and surface weights of 60 mg·cm^(-2)and 90 mg·cm^(-2),showing ignition delay times 10μs and 3μs,*** also measured detonation velocities for VS⁃2 composition film charges,which were 4375-4505 m·s^(-1)(of the charge being surface mass 60 mg·cm^(-2))and 4221-4281 m·s^(-1)(of the charge be⁃ing surface mass 90 mg·cm^(-2))and their blasting action on the aluminum *** depths of the normal shock wave imprints at the charge⁃barrier interface were 0.6-0.7 mm(for surface mass of the film charges 60 mg·cm^(-2))and 1.2-1.3 mm(for surface mass of the film charges 90 mg·cm^(-2))

关键词： VS⁃2 pyrotechnic composition flash lamps ignition transition of combustion to detonation blasting action

Hydromagnetic oscillatory Couette flow in rotating system with induced magnetic field

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Applied mathematics and mechanics(English Edition) 2014年第10期35卷 1331-1344页

作者： G.S.SETH S.m.HUSSAIN S.SARKAR Department of Applied mathematics Indian School of Mines Department of mathematics O.P.Jindal Institute of Technology

This paper presents a study of hydromagnetic Couette flow of an incompress- ible and electrically conducting fluid between two parallel rotating plates, one of which is oscillating in its own plane. A uniform transverse magnetic field is used, and the induced magnetic field is taken into account. The exact solution to the governing equations is obtained in a closed form. The solution to the problem in the case of vanishing and small finite magnetic Prandtl numbers is also derived from the general solution. The asymp- totic behavior of the solution for large values of the frequency parameter is analyzed to gain some physical insights into the flow pattern. Expressions for the shear stress at both the oscillatory and stationary plates due to primary and secondary flows and mass flow rate in the primary and secondary flow directions are also obtained. The results of the fluid velocity and the induced magnetic field are presented. The shear stresses on the plates due to the primary and secondary flows and the corresponding mass flow rates are presented in a tabular form.

关键词： magnetic interaction parameter Ekman number frequency parameter,hydromagnetic Stokes-Ekman boundary layer magnetic diffusion boundary layer

New Optical Soliton Solutions of the Perturbed Fokas-Lenells Equation

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Communications in Theoretical Physics 2019年第11期71卷 1275-1280页

作者： maha S.m.Shehata Hadi Rezazadeh Emad H.m.Zahran Eric Tala-Tebue Ahmet Bekir Departments of mathematics Zagazig UniversityFaculty of ScienceZagazigEgypt Faculty of Engineering Technology Amol University of Special Modern TechnologiesAmolIran Departments of mathematical and Physical Engineering Benha UniversityFaculty of EngineeringShubraEgypt Laboratoire D'Automatique et D'Informatique Appliquee(LAIA) IUT-FV of BandjounThe University of DschangBP 134BandjounCameroon Neighbourhood of Akcaglan Imarli StreetNumber:28/426030EskisehirTurkey

In this article, we employ the perturbed Fokas-Lenells equation(FLE), which represents recent electronic communications. The Riccati-Bernoulli Sub-ODE method which does not depend on the balance rule is used for thefirst time to obtain the new exact and solitary wave solutions of this equation. This technique is direct, effective and reduces the large volume of calculations.

关键词： perturbed Fokas-Lenells equation(FLE) Riccati-Bernoulli Sub-ODE method optical soliton solutions

Qualitative Land Suitability Evaluation for main Irrigated Crops in the Shahrekord Plain, Iran: A Geostatistical Approach Compared with Conventional method

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Pedosphere 2013年第6期23卷 767-778页

作者： Y.SAFARI I.ESFANDIARPOUR-BOROUJENI A.KAmALI m.H.SALEHI m.BAGHERI-BODAGHABADI College of Agriculture Vali-e-Asr University of Rafsanjan Department of Soil Science College of AgricultureVali-e-Asr University of Rafsanjan Department of Soil Science College of AgricultureShahrekord University

The purity of soil map units and their quality for various uses like land suitability evaluation are always *** main objective of this study was to compare the precision of qualitative land suitability classification based on geostatistical and conventional soil mapping methods for main irrigated crops in the Shahrekord Plain,Central Iran.A regular grid sampling method consisting 104sample points was designed and soil samples were *** kriged maps were achieved for all studied soil attributes after physico-chemical ***,to combine kriged maps and ecological requirements of the studied crops,a script was designed in ILWIS 3.4 software and consequently,kriged qualitative land suitability maps were *** qualitative land suitability was also mapped based on the representative pedon analysis in each soil map ***,comparison of the conventional and kriged maps was carried out using the statistical method,error *** results showed that the overall accuracies of wheat,sugar beet,potato and alfalfa maps were 39.8%,24.3%,18.7%and 18.6%at subclass category,respectively,whereas these values increased to80.9%,82.3%,23.7%and 82.3%at class level,***,it can be stated that thanks to the relative facility of conventional soil survey compared with geostatistical methods,this method can be expressed as a preferable way for handling a usual land suitability evaluation design;but using soil map units as land suitability delineations may lead to unsatisfactory results in estimation of quantity and type of existing limitations.

关键词： land use planning ordinary kriging representative pedon soil variability

No association of the cytotoxic T-lymphocyte associated gene CTLA4 +49A/G polymorphisms with Crohn's disease and ulcerative colitis in Hungarian population samples

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World Journal of Gastroenterology 2007年第15期13卷 2205-2208页

作者： Lili magyari Bernadett Faragó Judit Bene Katalin Horvatovich Lilla Lakner márta Varga mária Figler Beáta Gasztonyi Gyula mózsik Béla melegh Department of medical Genetics and Child Development University of Pécs PécsHungary mTA PTE Clinical Genetics Research Group of the Hungarian Academy of Sciences at the University of Pécs Pécs Hungary markusovszky Hospital Department of medicine and Gastroenterology SzombathelyHungary Réthy Pál Hospital 3rd Department of medicine and Gastroenterology BékéscsabaHungary Department of medicine School of medicine University of PécsPécsHungary Department of Gastroenterology Zala County Hospital ZalaegerszegHungary

AIm： The goal of the current work was to analyse the prevalence of the ＋49A/G variant of the cytotoxic T-lymphocyte antigen 4 gene （CTLA4） in Hungarian patients with Crohn＇s disease （CD） and ulcerative colitis （UC）. mETHODS： A total of 130 unrelated subjects with CD and 150 with UC, and 170 matched controls were genotyped for the single nucleotide polymorphism （SNP）. The genotypes were determined by using PCR/RFLP test. RESULTS： The G allele frequency and the prevalence of the GG genotype were 38.1% and 12.3% in the CD group, 40.6% and 18.6% in the UC patients, and 37.4% and 15.9% in the control group, respectively. CONCLUSION： The results of the current study show that carriage of the ＋49G SNP in heterozygous or in homozygous form does not confer risk either for CD or for UC in the Hungarian population.

关键词： Cytotoxic T-lymphocyte antigen 4 Crohn's disease Ulcerative colitis Inflammatory bowel disease

NDm-1 in the Indian environment:hitherto the problem is not disquieting

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Asian Pacific Journal of Tropical medicine 2012年第4期5卷 335-336页

作者： mohd Shahid Fatima Khan m Salman Shah Indu Shukla Fatima Shujatullah Haris m Khan Abida malik lqbal m Khan Section of Antimicrobial Agents & Drug Resistance Research and molecular Biology Department of Medical MicrobiologyJawaharlal Nehru Medical College & HospitalAligarh Muslim UniversityAligarh-202002Ultar PradeshIndia Department of Community medicine Jawaharlal Nehru Medical College & HospitalAligarh Muslim UniversityAligarh-202002Uttar PradeshIndia Section of Antimicrobial Agents & Drug Resistance Research and molecular Biology Department of Medical MicrobiologyJawaharlal Nehru Medical College & HospitalAligarh Muslim UniversityAligarh-202002Uttar PradeshIndia

blaNDm-1 is a recently described novel plasmid-mediated resis tance determinant that confes resistance to carbapenems. Bacteria harbouring NDm-1 have been reported from the clinical *** are also reported and warned as impending gut colonist,and recently described from the environment of the capital city of India,indicating the potential as a reservoir for future infections.

关键词： reservoir Indian Bacteria plasmid determinant latter isolates award preventive

Properties of carbon nanotube-dispersed Sr-hydroxyapatite injectable material for bone defects

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Regenerative Biomaterials 2016年第1期3卷 13-23页

作者： m.G.Raucci m.Alvarez-Perez D.Giugliano S.Zeppetelli L.Ambrosio Institute of Polymers Composites and BiomaterialsNational Research Council of ItalyMostra D’oltremare Pad.20-Viale Kennedy 54Naples 80125Italy Tissue Bioengineering Laboratory DEPeIFaculty of DentistryNational Autonomous University of MexicoMexico DF 04510Mexico Department of Chemical Sciences and materials Technology National Research Council of Italy(DSCTM-CNR)P.Le Aldo Moro 7Rome 00185Italy

This study concerns the synthesis of gel materials based on carbon nanotubes dispersed strontium-modified hydroxyapatite(Sr-HA)at different compositions obtained by sol–gel technology and their influence on human-bone-marrow-derived mesenchymal stem ***,an evaluation of the influence of nanotubes and Strontium on physico-chemical,morphological,rheological and biological properties of hydroxyapatite gel was also *** analysis(scanning electron microscopy)shows a homogeneous distribution of modified nanotubes in the ceramic matrix improving the bioactive properties of *** biological investigations proved that Sr-HA/carbon nanotube gel containing 0–20 mol(%)of Sr showed no toxic effect and promote the expression of early and late markers of osteogenic differentiation in cell culture performed in basal medium without osteogenic ***,the SrHA/carbon nanotube gels could have a good potential application as filler in bone repair and regeneration and may be used in the osteoporotic disease treatment.

关键词： nanobiomaterials sol-gel tecnology biomaterial-cell interaction bioceramics bone tissue engineering