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Genotype-environment interaction in Cordia trichotoma(Vell.)Arrá*** *** in two different soil conditions

Journal of Forestry Research 2022年第1期33卷 309-319页

作者： Wanderley dos Santos Bruno marchetti de Souza Daniele Fernanda Zulian Gilberto Terra Ribeiro Alves João Bosco Vasconcellos Gomes mário luiz teixeira de moraes Valderês Aparecida de Sousa Ananda Virginia de Aguiar Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista“Júlio Mesquita Filho”Ilha Solteira 15385-000Brazil Sucupira Agroflorestas Fazenda SucupiraValença 45400-000Brazil EmBRAPA Florestas Empresa Brasileira de Pesquisa AgropecuáriaColombo 83411-000Brazil

Investment in silvicultural techniques is noticeably lacking,especially in breeding programs for non-conventional wood *** genotype×environment interaction(G×E)is essential to the development of breeding ***,this study aimed to estimate genetic diversity of and the effects of G×E interaction on two progeny tests of Cordia trichotoma,including the estimation of genetic gain and genetic diversity after *** the experiment,30 progenies of *** were tested at two sites with differing soil *** at breast height(1.30 m above soil surface,dbh-cm),total height,diameter at 30 cm from the soil,first branch height,and survival were all monitored for four *** deviance,best linear unbiased estimator,and harmonic mean of relative performance of genetic values(mHPRVG)were all calculated to predict breeding values,estimate genetic parameters,and analyze *** quantified traits varied significantly among progenies by soil type,with greatest variation recorded for genetic *** of the progenies led to predictions of genetic gain,ranging from 7.73 to 15.45%,for dbh at four years of *** calculated decrease in genetic diversity after selection showed that this parameter should be monitored in subsequent breeding cycles.G×E was low for all *** best-performing progenies proved most stable and best adapted to the different environmental conditions tested.

关键词： Effective population number Native species Productivity Stability and adaptability Tree improvement

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delay Times and Clinical Outcomes in Acute myocardial Infarction: Comparison of Periods before and during the COVID-19 Pandemic—myocardial Infarction and the Pandemic

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World Journal of Cardiovascular Diseases 2024年第6期14卷 392-400页

作者： Clarice teixeira Silva Araújo Arnon Salviato mameri Thúlio Carrera Guarçoni Venturini marcus Tadeu Aguilar Constantino matos Igor morais Araujo Lopes luiz Gustavo Ribeiro de Carvalho murad Cleberson Duartes Ovani Guilherme Vassalo morais Fernanda Venturini de Castro Danielle Lopes Rocha Lucas Crespo de Barros Rodolfo Costa Sylvestre luiz Fernando machado Barbosa Roberto Ramos Barbosa Internal medicine Universidade Federal do Esprito Santo (UFES) Vitria Brazil Hospital Santa Casa de misericrdia de Vitria Vitria Brazil Hospital Santa Rita de Cssia Vitria Brazil Hospital Estadual Dr. Jayme Santos Neves Serra Brazil Grupo Kora Sade Vitria Brazil Hospital Estadual Infantil e maternidade Alzir Bernardino Alves (HImABA) Vila Velha Brazil

Introduction: At the beginning of the COVID-19 pandemic, a drop in the number of patients treated for cardiac emergencies raised concern about cardiovascular mortality in that period. An increase in care delay for patients with ST-segment elevation myocardial infarction (STEmI) may have affected clinical outcomes. Objectives: To analyze delay times and clinical outcomes of patients with STEmI undergoing primary percutaneous coronary intervention (PPCI), before and during the COVID-19 pandemic. methods: Retrospective observational study that included patients with STEmI undergoing PPCI from december 2018 to July 2021. The COVID-19 pandemic cases were divided into two groups: pandemic I—from march to August 2020;and pandemic II—from September 2020 to July 2021. Patients were compared according to the period of hospitalization. Primary outcomes were delay times in assistance and clinical outcomes (acute kidney injury [AKI], post-procedural vascular complications and in-hospital mortality). Results: 108 patients were included, 39 (36.1%) in the pre-pandemic period, 13 (12.1%) in pandemic I and 56 (51.8%) in pandemic II. Time from onset of symptoms to arrival at the service and door-to-balloon time did not differ significantly among groups. Vascular complications were more frequent during the pandemic (I and II) than in the pre-pandemic period (2.5% pre-pandemic vs 15.4% pandemic vs 12.5% pandemic II;p = 0.03). AKI incidence was similar in all three periods. There was a non-significant increase in in-hospital mortality during the COVID-19 pandemic. Conclusion: In patients with STEmI, there was an increase in vascular complications and a trend toward increased mortality during the COVID-19 pandemic. delay times to admission and reperfusion did not differ significantly between before and during the pandemic.

关键词： myocardial Infarction Primary Percutaneous Coronary Intervention Coronary Reperfusion Pandemic COVID-19

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development of a Ready-to-Use PSmA-11 Kit Formulation and Biological Evaluation of Binding Affinity

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Advances in molecular Imaging 2024年第2期13卷 7-22页

作者： maria Renata Valente Brandão Freire Natália marchesan Bexiga marco Antonio Stephano João Ezequiel Oliveira Carlos Roberto Jorge Soares Ana Cláudia Camargo miranda Anna Carolina de Alcântara Barbosa marycel Figols de Barboza Laura Fernanda Garcia luiz Felipe S. teixeira maria Helena marumo Bellini Natanael Gomes da Silva luiza mascarenhas Balieiro Joel mendes Elaine Bortoleti de Araújo Centro de Radiofarmcia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil departamento de Tecnologia Farmacutica Faculdade de Cincias Farmacuticas (USP) Cidade Universitria So Paulo Brasil Centro de Biotecnologia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil departamento de medicina Nuclear do Hospital Israelita Albert Einstein So Paulo/SP Brasil

Introduction: 68Ga-PSmA-11 is considered the gold standard in detection of micro and oligometastases in advanced prostate cancer, being used for therapeutic planning, as well as, potentially, for evaluating response to treatment. The development of ready-to-use lyophilized kit of PSmA-11 adds quality and safety to the routine use of this radiopharmaceutical and represents a pharmacotechnical challenge as it must preserve the integrity and specificity of the ligand. methods: PSmA-11 kit formulation was proposed, considering radiolabeling parameters and the preservation of the peptide during the lyophilization process, using mannitol as an excipient. Critical temperature characterization studies were carried out using DSC equipment and the freeze-drying process was developed. The direct radiolabeling conditions were evaluated and standardized using 68Ge/68Ga generator eluate from two different manufacturers (ITG and Eckert & Ziegler). The radiochemical purity was evaluated by TLC and HPLC. Biological evaluation was carried out with lyophilized PSmA-11 to demonstrate the integrity of the peptide and preservation of biological activity after the lyophilization process. Results: Based on critical temperature characterization studies, the freeze-drying cycle was designed to reach a freezing temperature of around −40˚C and primary drying at 2˚C. Using 20 mg of mannitol, an intact and elegant lyophilized cake was obtained. PSmA-11 lyophilized kit was directly labeled with 68Ga eluate from 68Ge/68Ga GmP generators (ITG and Eckert & Ziegler) resulting in % RP > 95% at pH 4.0 to 4.5. The results obtained from in vitro and in vivo biological competition studies confirmed the preservation of PSmA-11 affinity for the receptor after lyophilization. Conclusion: A lyophilized formulation (Kit) of PSmA-11 was successfully obtained, which preserved the integrity and biological activity of the peptide and guaranteed radiolabeling efficiency.

关键词： 68Ga-PSmA-11 Prostate-Specific membrane Antigen (PSmA) Ready-to-Use Kit GmP-Grade Generators metastatic Prostate Cancer PET Imaging

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Application of data science in the prediction of solar energy for the Amazon basin:a study case

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Clean Energy 2023年第6期7卷 1344-1355页

作者： AndréLuis Ferreira marques márcio Joséteixeira Felipe Valencia de Almeida Pedro luiz Pizzigatti Corrêa Polytechnic School of the University of Sao Paulo Av.Prof.Luciano Gualberto380-ButantaSao Paulo-SP05508-010Brazil Institute of Physics of the University of Sao Paulo Brazil

The need for renewable energy sources has challenged most countries to comply with environmental protection actions and to handle climate *** energy figures as a natural option,despite its *** has a green energy matrix with significant expansion of solar form in recent *** preserve the Amazon basin,the use of solar energy can help communities and cities improve their living standards without new hydroelectric units or even to burn biomass,avoiding harsh environmental *** novelty of this work is using data science with machine-learning tools to predict the solar incidence(W.h/m^(2))in four cities in Amazonas state(north-west Brazil),using data from NASA satellites within the period of ***-tree-based models and vector autoregressive(time-series)models were used with three time aggregations:day,week and *** predictor model can aid in the economic assessment of solar energy in the Amazon basin and the use of satellite data was encouraged by the lack of data from ground *** mean absolute error was selected as the output indicator,with the lowest values obtained close to 0.20,from the adaptive boosting and light gradient boosting algorithms,in the same order of magnitude of similar references.

关键词： solar energy renewable energy Amazon basin machine learning time series data science decision-trees ensemble vector autoregression

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Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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Journal of Forestry Research 2022年第5期33卷 1573-1584页

作者： Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz molinari Rafaella Zanetti Dias Gustavo Leal teixeira Antônio Natal Gonçalves marcílio de Almeida Institute of Agricultural Science Federal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest Species Department of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil department of Biological Sciences “Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil

Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a murashige and Skoog(mS)culture medium supplemented with NAA or *** tissues were cultivated in a mS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPm culture medium supplemented with 0.89μm BAP and 0.05μm *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μm BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.

关键词： micropropagation Recalcitrant species Plant growth regulator Explants source

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Lafoensia pacari alleviates intestinal damage by modulating cyclooxygenase-2:In silico and in vivo evaluation in a colitis model

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World Journal of Gastroenterology 2023年第17期29卷 2628-2641页

作者： Gabrielle Caroline Peiter Thayene Kamyli moesch Queiroz Edson luiz michalkiewicz Jr Raphael Henrique Chappuis Jennefer Sousa Luz luiz Henrique Casagrande Piovezani Cleison Ferreira Silva matheus Nozomi Tsutumi Augusto Fernandes Chaves Rafael messias luiz Cinthia Façanha Wendel Ana Carla Zarpelon-Schutz Kádima Nayara teixeira Programa multicêntrico de Pós-graduação em Biquímica e Biologia molecular-Setor Palotina Universidade Federal do ParanáPalotina 85.950-000ParanáBrazil Programa de Pós-graduação em Biotecnologia-Setor Palotina Universidade Federal do ParanáPalotina 85.950-000ParanáBrazil Campus Toledo Universidade Federal do ParanáToledo 85.919-899ParanáBrazil

BACKGROUND Inflammatory bowel diseases(IBD)are a worldwide health problem and mainly affect young people,consequently affecting the *** treatments are often associated with side effects,and new therapeutic options are *** centuries,plants have represented important substrates in the field of drug *** pacari(***)is a plant whose pharmaceutical potential has been described,and may have biological activity relevant to the treatment of IBD *** To investigate the activity of keto-alcoholic extracts of *** with respect to ameliorating the inflammatory and nociceptive symptoms of acute experimental colitis in *** Keto-alcoholic extracts of *** leaves and bark were administered to male andfemale Swiss mice weighing 25 g to 30 g(n=8 male mice and n=8 female mice).The effect of these extracts was observed in an acetic acid-induced acute experimental model of colitis with regard to antinociception/analgesia and inflammatory tissue *** macroscopic indices included the Wallace score and the colon weight obtained using a precision *** hyperalgesia was determined using an electronic *** related to overt pain was determined by quantifying the number of writhing instances within 20 min of administration of acetic *** docking was performed using human and murine cyclooxygenase-2(COX-2)with 3 flavonoids(ellagic acid,kaempferol,and quercetin)on the AutoDock Vina *** of variance followed by Tukey’s posttest was used with Pmurine model of colitis,administration of extracts from *** ameliorated acetic acidinduced writhing and colitis-associated inflammatory *** improvements may be attributable to the reduction in edema,inflammation(e.g.,ulcers,hyperemia,and bowel wall damage),and the intensity of abdominal *** keto-alcoholic extracts of *** leaves and bark administered at a

关键词： Antinociceptive activity Anti-inflammatory activity Cyclooxygenase-2 Flavonoids Inflammatory bowel disease Lafoensia pacari

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Numerical Study on the Effect of Submerged depth on the Horizontal Plate Wave Energy Converter

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China Ocean Engineering 2014年第5期28卷 687-700页

作者： Flávio medeiros SEIBT Eduardo Costa COUTO Elizaldo Domingues dos SANTOS Liércio André ISOLDI luiz Alberto Oliveira ROCHA Paulo Roberto de Freitas teixeira Graduate Program of Ocean Engineering Universidade Federal do Rio Grande96203-900 Rio Grande RS Brazil mechanical Engineering department Universidade Federal do Rio Grande do Sul90050-170 Porto Alegre RS Brazil

The growing search for clean and renewable energy sources has given rise to the studies of exploring sea wave energy. This paper is concerned with the numerical evaluation of the main operational principle of a submerged plate employed for the conversion of wave energy into electrical one. The numerical model used to solve the conservation equations of mass, momentum and transport of volume fraction is based on the finite volume method （FVm）. In order to tackle with the flow of mixture of air-water and its interaction with the device, the multiphase model volume of fluid （VOF） is employed. The purpose of this study is the evaluation of a numerical model for improvement of the knowledge about the submerged plate wave energy converter, as well as the investigation of the effect of the distance from the plate to the bottom of the sea （HP） on the performance of the converter. The simulations for several distances of the plate from the seabed show that the optimal efficiency is 64%, which is obtained for HP=0.53 m （88% of the depth）. This efficiency is 17% larger than that found in the worst case （HP=0.46 m, 77% of the depth）.

关键词： numerical simulation submerged plate wave energy conversion efficiency

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Liver transplant in patients with primary sclerosing cholangitis:A retrospective cohort from Northeastern Brazil

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世界肝病学杂志:英文版(电子版) 2023年第9期15卷 1033-1042页

作者： Louyse teixeira de Souza Freitas Elodie Bomfim Hyppolito Victor Leonardo Barreto luiz Humberto JataíCastelo Júnior Bianca Carneiro de melo Jorge Frederico César Tahim de Sousa Brasil Háteras malthus Barbosa marzola Clébia Azevedo Lima Raquel mendes Celedonio Gustavo Rêgo Coelho Jose Huygens Parente Garcia Liver Transplant department Walter Cantídio Teaching HospitalFortaleza 60430270CearáBrazil School of medicine University of FortalezaFortaleza 60811905CearáBrazil Hospital São José CearáState Health DepartmentFortaleza 60455610CearáBrazil department of Surgery Federal University of CearáFortaleza 60430140CearáBrazil Surgery department São Carlos HospitalFortaleza 60130241CearáBrazil

BACKGROUND Primary sclerosing cholangitis(PSC)manifests within a broad ethnic and racial spectrum,reflecting different levels of access to health *** To evaluate the clinical profile,complications and survival rates of patients with PSC undergoing liver transplantation(LTx)at a Brazilian reference *** All patients diagnosed with PSC before or after LTx were *** medical records were reviewed for demographic and clinical variables,including outcomes and *** level of statistical significance was set at Pmale(n=19;56%).The average age(40±14 years)was similar for men and women(P=0.347).The mean follow-up time from diagnosis to LTx was 68 *** patients had the classic form of *** women had PSC/autoimmune hepatitis overlap syndrome,and one patient had small-duct *** phosphatase levels at diagnosis and pre-LTx model for end-stage liver *** were significantly higher in *** bowel research(IBD)was investigated by colonoscopy in 26/34(76%)and was present in most cases(18/26;69%).IBD was less common in women than in men(44.4%vs.55.6%)(P=0.692).Cholangiocarcinoma(CCA)was diagnosed in 2/34(5.9%)patients by histopathology of the explant(survival:3 years 6 mo,and 4 years 11 mo).Two patients had complications requiring a second LTx(one after 7 d due to hepatic artery thrombosis and one after 17 d due to primary graft dysfunction).Five patients(14.7%)developed biliary *** overall median post-LTx survival was 66 *** deaths occurred in the first year(infection n=2,primary liver graft dysfunction n=3,unknown cause n=1).The 1-year and 5-year survival rates of this cohort were 82.3%and 70.6%,respectively,matching the mean overall survival rates of LTx patients at our center(87.1%and 69.43%,respectively)(P=0.83).CONCLUSION Survival after 1 and 5 years was simil

关键词： Primary sclerosing cholangitis Epidemiology Liver transplantation Survivor Clinical associations Pathological features

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multi-omics Analysis of Young Portulaca oleracea ***’Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species

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Phenomics 2023年第1期3卷 1-21页

作者： Vivianny Nayse Belo Silva Thalliton luiz Carvalho da Silva Thalita massaro malheiros Ferreira Jorge Candido Rodrigues Neto AndréPereira Leão JoséAntônio de Aquino Ribeiro Patrícia Verardi Abdelnur Leonardo Fonseca Valadares Carlos Antônio Ferreira de Sousa manoel teixeira Souza Júnior Graduate Program of Plant Biotechnology Federal University of LavrasCP 3037LavrasMG 37200-000Brazil Institute of Chemistry Federal University of GoiásCampus SamambaiaGoiâniaGO 74690‐900Brazil Brazilian Agricultural Research Corporation Embrapa Mid-NorthTeresinaPI 64008-780Brazil Brazilian Agricultural Research Corporation Embrapa AgroenergyBrasíliaDF 70770‐901Brazil

Soil salinity is among the abiotic stressors that threaten agriculture the most,and purslane(Portulaca oleracea L.)is a dicot species adapted to inland salt desert and saline habitats that hyper accumulates salt and has high phytoremediation *** researchers consider purslane a suitable model species to study the mechanisms of plant tolerance to drought and salt ***,a robust salinity stress protocol was developed and used to characterize the morphophysiological responses of young purslane plants to salinity stress;then,leaf tissue underwent characterization by distinct omics platforms to gain further insights into its response to very high salinity *** salinity stress protocol did generate different levels of stress by gradients of electrical conductivity at field capacity and water potential in the saturation extract of the substrate,and the morphological parameters indicated three distinct stress *** expected from a halophyte species,these plants remained alive under very high levels of salinity stress,showing salt crystal-like structures constituted mainly by Na^(+),Cl^(−),and K^(+)on and around closed stomata.A comprehensive and large-scale metabolome and transcriptome single and integrated analyses were then employed using leaf *** multi-omics integration(mOI)system analysis led to a data-set of 51 metabolic pathways with at least one enzyme and one metabolite differentially expressed due to salinity *** data sets(of genes and metabolites)are valuable for future studies aimed to deepen our knowledge on the mechanisms behind the high tolerance of this species to salinity *** conclusion,besides showing that this species applies salt exclusion already in young plants to support very high levels of salinity stress,the initial analysis of metabolites and transcripts data sets already give some insights into other salt tolerance mechanisms used by this species to support high levels of salinity stress.

关键词： Purslane RNA-Seq Chemometrics High-resolution mass spectrometry Abiotic stress multi-omics integration

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Genetic Variability in <i>myracrodruon urundeuva</i>(Allemão) Engl. Progeny Tests

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Open Journal of Forestry 2017年第1期7卷 1-10页

作者： Daniela Silvia de Oliveira Canuto Alexandre marques da Silva miguel luiz menezes Freitas Alexandre magno Sebbenn mario luiz teixeira de moraes Ilha Solteira School of Engineering S&#227o Paulo State University (UNESP) S&#227o Paulo Brazil Forestry Institute S&#227o Paulo Brazil

Aroeira (myracrodruon urundeuva) is a widely spread tree species, but due to its excellent wood quality it is now part of the list of vulnerable trees for extinction. Thus, strategies for the conservation of the genetic variability of the remaining natural populations are needed. Therefore, this study aimed to evaluate the genetic variability in m. urundeuva progeny tests, from natural populations located in areas with and without human disturbance. Seeds of six natural populations of m. urundeuva were collected from two conditions, with and without anthropogenic disturbance, and nine progeny tests were installed in the Education, Research and Extension Farm, located in Selvíria, mato Grosso do Sul State, Brazil. The overall height, average crown diameter (DmC) and the diameter at breast height (DBH) were assessed as phenotypic traits. Estimates of variance components and genetic parameters were obtained by REmL/BLUP method (maximum restricted likelihood/best unbiased linear prediction). The results showed that natural population with human disturbance presented less genetic variability than populations originating from areas without human disturbance (CVr until 0.87). The progeny tests evaluated had high accuracy (0.91 for DBH), which ensures the viability of the ex situ genetic conservation bank and its successful use in the breeding program, i.e., in the conversion of the progeny tests into m. urundeuva seed orchards. The most suitable character for selection was the DBH.

关键词： Conservation Improvement Seed Orchard

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