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Gut Microbiota Dysbiosis Strengthens Kupffer Cell-mediated Hepatitis B Virus Persistence through Inducing Endotoxemia in Mice

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Journal of Clinical and Translational Hepatology 2022年第1期10卷 17-25页

作者： Wenqing Zhou jinzhuo luo Xiaohong Xie Shangqing Yang Dan Zhu Hongming Huang Dongliang Yang Jia Liu Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina

Background and Aims:Change of gut microbiota com-position is associated with the outcome of hepatitis B virus(HBV)infection,yet the related mechanisms are not fully *** objective of this study was to investigate the immune mechanism associated with HBV persistence induced by gut microbiota ***:C57BL/6 mice were sterilized for gut-microbiota by using an antibi-otic(ABX)mixture protocol,and were monitored for their serum endotoxin(lipopolysaccharide[LPS])*** HBV-replicating mouse model was established by performing HBV-expressing plasmid pAAV/HBV1.2 hydrodynamic injec-tion(HDI)with or without LPS,and was monitored for se-rum hepatitis B surface antigen,hepatitis B e antigen,HBV DNA,and cytokine *** cells(KCs)were purified from antibiotic-treated mice and HBV-replicating mice and analyzed for IL-10 production and T cell suppression ***:ABX treatment resulted in increased serum LPS levels in *** KCs separated from both ABX-treated and LPS-treated HBV-replicating mice showed significantly increased IL-10 production and enhanced ability to suppress IFN-γproduction of TCR-activated T cells than the KCs sep-arated from their counterpart *** of pAAV/HBV1.2 in combination with LPS in mice led to a delayed HBV clear-ance and early elevation of serum IL-10 levels compared to pAAV/HBV1.2 HDI ***,IL-10 function blockade or KC depletion led to accelerated HBV clearance in LPS-treated HBV-replicating ***:Our results suggest that dysbiosis of the gut microbiota in mice leads to endotoxemia,which induces KC IL-10 production and strengthens KC-mediated T cell suppression,and thus fa-cilitates HBV persistence.

关键词： Chronic hepatitis B Gut microbiota Kupffer cells T cell IL-10

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HBeAg induces liver sinusoidal endothelial cell activation to promote intrahepatic CD8 T cell immunity and HBV clearance

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Cellular & Molecular Immunology 2021年第11期18卷 2572-2574页

作者： Xiaohong Xie jinzhuo luo Ruth Broering Dan Zhu Wenqing Zhou Mengji Lu Xin Zheng Ulf Dittmer Dongliang Yang Jia Liu Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina Department of Gastroenterology and Hepatology University Hospital of EssenUniversity of Duisburg-Essen45122EssenGermany Institute for Virology University Hospital of EssenUniversity of Duisburg-Essen45122EssenGermany

To the Editor It is believed that the inherent tolerogenic property of the liver is involved in the chronicity of hepatitis B virus(HBV)infection[1].However,exposure to HBV in adults usually leads to spontaneous clearance of the virus and the induction of potent and effective anti-HBV T cell immunity in the liver[2],suggesting that the immune microenvironment of the liver switches from limiting to allowing effector T cell responses during acute resolution of HBV infection.

关键词： immunity liver acute

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Induction of liver sinusoidal endothelial cells immune activation by HBeAg during HBV infection

Induction of liver sinusoidal endothelial cells immune activ...

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第十二届全国免疫学学术大会

作者： Yu Qing luo jinzhuo Wang Qin Yang Shangqing Yang Dongliang Liu Jia Department of Infectious Diseases Institute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology

Backgrounds & Aims:Liver sinusoidal endothelial cells(LSECs) play a crucial role in maintaining the homeostasis of the hepatic microenvironment through induction of Ag-specific T cell *** has been shown that some viruses infection or TLRs stimulation can induce the maturation of LSECs,which reverts their suppressive properties to induce T cell ***,little is known about whether and how the function of LSECs in regulating T cell immunity is regulated during HBV ***,we investigated the function and mechanism of LSECs regulating CD8 T cell immunity during HBV ***:Mice were hydrodynamic injected with pAAV/HBV1.2 or pSM2,and their LSECs were freshly isolated at different time points post injection and co-cultured with polyclonal TCR-activated T *** freshly isolated from naive mice were stimulated by recombinant HBV antigen protein and co-cultured with polyclonal TCR-activated T *** phenotype and function of LSECs were characterized by flow cytometry,quantitative PCR,and functional ***:Compared to LSECs of naive mice or HI control mice,LSECs separated from HBV HI mice at 14 dpi reverted their suppressive properties to enhance T cell *** analysis demonstrated HBeAg stimulation could induce the maturation of naive LSECs and enable them to promote IFN-γ production by TCR-activated CD8 T *** separated from HBeAg-deficiency HBV HI mice were less capable of promoting T cell immunity compared to those separated from wild type HBV HI *** contrast,replenishing HBeAg expression in HBeAg-deficiency HBV HI mice resulted in better LSEC activation and led to accelerated HBV *** LSECs separated from HBV HI mice and HBeAg stimulated LSECs showed increased TNF-α ***:HBV replication can revert LSECs suppressive properties to promote T cell immunity,which is probably mediated by HBeAg induced LSECs TNF-α production.

关键词： Hepatitis B Liver Sinusoidal Endothelial Cells T cell immunity

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Kinetics analysis of the function of splenocytes from acuteresolving HBV-mice in breaking HBV tolerance

Kinetics analysis of the function of splenocytes from acuter...

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第十一届全国免疫学学术大会

作者： Wang Qin Liu Yanan Pan Wen Yu Qing luo jinzhuo Yang Shangqing Wang Lu Zhu Dan Liu Jia Yang Dongliang Union Hospital Tongji Medical CollegeHuazhong University of Science and Technology

Background: Effective adaptive immune responses are vital for the clearance of HBV infection. However, little is known about how the antiviral function of different immune cells of peripheral lymphoid organs changes during the course of acute-resolving HBV infection. Here, we investigated the kinetics of functional change of different splenocytes from acute-resolving HBV-mice in breaking HBV tolerance using HBV hydrodynamic injection(HI) mouse ***: HI was performed to establish acute-resolving( p SM2 plasmid) or persisting(p AAV/HBV1.2 plasmid) HBV replication in CD45.1 and CD45.2 C57BL/6 mice. Total splenocytes, splenic CD8+ T cells and splenocytes without CD8+ T cells(non-CD8) were purified from HBV acute-resolving mice at different time points post virus exposure and were adoptively transferred to HBV persistence mice. The viremia in the recipient mice was monitored. The phenotype and function of donor immune cells were analyzed by flow ***: Viral clearance was achieved in HBV persistence mice by adoptive transfer of 14dpi(days post injection) total splenocytes and 21 dpi purified splenic CD8+ T cells from acute-resolving HBV-mice. Besides, adoptive transfer of 7dpi non-CD8, 14 dpi CD8+ T cells and non-CD8, 21 dpi total splenocytes, and 28 dpi non-CD8 led to a better control of viral replication. Interestingly, adoptive transfer of 21 dpi non-CD8 and 28 dpi CD8+ T cells resulted in a delayed HBV clearance. We observed increased CD8+ T cell infiltration in the liver in 14 dpi total splenocytes and 21 dpi CD8+ T cell transferred group. These CD8+ T cells showed an activation phenotype and increased IFN-γ production compared to that in other cells transferred ***: The antiviral function varies in different splenocytes as well as at different time points during the course of acute-resolving HBV replication. The achievement of breaking HBV tolerance relies on the activation of both splenic CD8+ T cells and non-CD8 cells. The acti

关键词： HBV tolerance adoptive transfer splenocytes CD8+ T cells

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Function and Mechanism Analysis of Liver Endothelial Cells

Function and Mechanism Analysis of Liver Endothelial Cells

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第十一届全国免疫学学术大会

作者： Yu Qing luo jinzhuo Wang Qin Liu Yanan Wang Lu Yang Shangqing Zhu Dan Pan Wen Liu Jia Yang Dongliang Department of Infectious Diseases Institute of Infection and Immunology Union Hospital Tongji Medical College Huazhong University of Science and Technology

Backgrounds&Aims:Liver sinusoidal endothelial cells(LSECs)play a crucial role in maintaining the homeostasis of the hepatic microenvironment through induction of Ag-specific T cell ***,certain virus infection can induce the maturation of *** far,little is known about whether or how the function of LSECs in regulat-

关键词： Hepatitis B virus Liver sinusoidal endothelial cell T cell immunity PD-L1

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Regulation of antiviral CD8 T cell response by Sema4D/CD100 and its soluble form in HBV infection

Regulation of antiviral CD8 T cell response by Sema4D/CD100 ...

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第十一届全国免疫学学术大会

作者： Yang Sahngqing Wang Lu Liu Yanan Wang Qin Yu Qing luo jinzhuo Bayer Wibke Dittmer Ulf Lu Mengji Yang Dongliang Liu Jia Department of Infectious Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Department of Gastroenterology University Hospital of Essen University of Duisburg-Essen Institute for Virology University Hospital of Essen University of Duisburg-Essen

Backgroud:Sema4D/CD100 was the first semaphorin described to have immune functions and serves important roles in T cell priming,antibody *** cleavage of membrane CD100 gives rise to a soluble fragment of CD100(s CD100),which also have immunoregulatory *** we characterized the expression and the possible role of CD100/s CD100 in regulating antiviral response during HBV infection in patients and HBVreplicating mouse model.

关键词： hepatitis B virus Cytotoxic T lymphocytes CD100

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武汉方正金融中心空调系统设计与分析

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暖通空调 2022年第10期52卷 55-61页

作者：罗金卓徐鸿曹涛中南建筑设计院有限公司武汉

介绍了武汉方正金融中心项目冷热源、风系统、水系统的设计方案,分析了办公区域高效制冷机房的具体技术措施。通过比较不同方案的系统能效和经济性,最终确定办公区域采用低温送风全空气变风量(VAV)系统,其中空调内区为单风道型VAV,外区... 详细信息

介绍了武汉方正金融中心项目冷热源、风系统、水系统的设计方案,分析了办公区域高效制冷机房的具体技术措施。通过比较不同方案的系统能效和经济性,最终确定办公区域采用低温送风全空气变风量(VAV)系统,其中空调内区为单风道型VAV,外区为热水再热盘管并联型风机动力型变风量末端(FPB);商业区域单独设置冷源,空调水系统采用外区两管制、内区四管制的分区四管制系统。过渡季和冬季通过冷却塔供冷,降低系统运行费用。

关键词：综合体冷热源大温差换热全空气系统冷却塔变风量分区四管制系统

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Recommender System Combining Popularity and Novelty Based on One-Mode Projection of Weighted Bipartite Network

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Computers, Materials & Continua 2020年第4期63卷 489-507页

作者： Yong Yu Yongjun luo Tong Li Shudong Li Xiaobo Wu jinzhuo Liu Yu Jiang School of Software Key Laboratory in Software Engineering of Yunnan ProvinceYunnan UniversityKunming650091China School of Big Data Yunnan Agricultural UniversityKunming650201China Cyberspace Institute of Advanced Technology Guangzhou UniversityGuangzhou510006China School of Computer Science and Cyber Engineering Guangzhou UniversityGuangzhou510006China

Personalized recommendation algorithms,which are effective means to solve information overload,are popular topics in current *** this paper,a recommender system combining popularity and novelty(RSCPN)based on one-mode projection of weighted bipartite network is *** edge between a user and item is weighted with the item’s rating,and we consider the difference in the ratings of different users for an item to obtain a reasonable method of measuring the similarity between *** can be used in the same model for popularity and novelty recommendation by setting different parameter values and analyzing how a change in parameters affects the popularity and novelty of the recommender *** verify and compare the accuracy,diversity and novelty of the proposed model with those of other models,and results show that RSCPN is feasible.

关键词： Personalized recommendation one-mode projection weighted bipartite network novelty recommendation diversity

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构建泌尿外科腔内手术围手术期低体温风险预测模型的横断面研究

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皖南医学院学报 2023年第6期42卷 549-553页

作者：罗元元程帆蔡忠香宁金卓武汉大学人民医院泌尿外科湖北武汉430000 皖南医学院第一附属医院弋矶山医院重症监护室安徽芜湖241001 武汉大学人民医院护理部湖北武汉430000

目的:构建泌尿外科腔内手术患者术中发生低体温风险预测模型,量化其预测效能。方法:选择弋矶山医院2022年5~8月129例泌尿外科择期行泌尿系统腔内手术的患者为研究对象,分析泌尿外科腔内手术患者术中发生低体温的独立影响因素,构建围手... 详细信息

目的:构建泌尿外科腔内手术患者术中发生低体温风险预测模型,量化其预测效能。方法:选择弋矶山医院2022年5~8月129例泌尿外科择期行泌尿系统腔内手术的患者为研究对象,分析泌尿外科腔内手术患者术中发生低体温的独立影响因素,构建围手术期低体温的风险预测模型。结果:围手术期低体温组和体温正常组的BMI、腔内手术类型、术前MAP、术前HR、术前体温、术前RBC、全麻、手术时间、围手术期冲洗量、围手术期失血量差异均有统计学意义(P0.05)。结论:泌尿外科腔内手术围手术期低体温发生风险预测模型由术前MAP、全麻、手术时间、围手术期冲洗量等因素构成。该预测模型经量化为优化评分,展现出良好的评判效能。

关键词：泌尿外科腔内手术围手术期低体温

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基于围手术期低体温的影响因素构建管理策略

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中华全科医学 2024年第11期22卷 1934-1937,1949页

作者：罗元元程帆蔡忠香宁金卓姚卫东武汉大学人民医院泌尿外科湖北武汉430000 皖南医学院第一附属医院弋矶山医院重症监护室安徽芜湖241000 皖南医学院第一附属医院弋矶山医院麻醉科

围手术期低体温(核心温度<36℃)是手术患者常见的并发症。一些以患者为中心的外部因素,如药物、合并症、创伤、环境温度、麻醉类型以及手术的范围和持续时间,均会影响核心体温。本文总结分析了麻醉前、麻醉中、麻醉后影响体温的各种因... 详细信息

围手术期低体温(核心温度<36℃)是手术患者常见的并发症。一些以患者为中心的外部因素,如药物、合并症、创伤、环境温度、麻醉类型以及手术的范围和持续时间,均会影响核心体温。本文总结分析了麻醉前、麻醉中、麻醉后影响体温的各种因素以及围手术期低体温的不良后果。围手术期低体温不仅对人体各器官产生不良影响,还会减慢麻醉药物和止痛药物的代谢速度,导致麻醉效果不佳和副作用增加。同时,低体温还会抑制凝血酶原的活化,增加术后出血的风险。此外,低体温还会抑制机体的免疫功能,降低白细胞的活性,从而降低抵抗感染的能力,增加手术部位感染的概率。因此,在围手术期进行良好的体温管理至关重要。住院期间对患者进行良好的体温管理,可以降低围手术期低体温的发生风险。体温测量应使用准确、连续性探头,避免结果出现误差。围手术期体温管理包括一套适合个人需求的保温工具,保暖毯和床垫以及专用设备给予适当加热的输液非常重要。体温管理应遵循检查清单,并根据患者的要求和当地的可能性进行个体化。本研究整理了国内外关于围手术期低体温的不良后果、危险因素及预防策略等相关文献,以期为防治围手术期低体温提供可靠的方案。

关键词：低体温围手术期手术体温管理

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