Induction of liver sinusoidal endothelial cells immune activation by HBeAg during HBV infection
作者单位：Department of Infectious DiseasesInstitute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology
会议名称：《第十二届全国免疫学学术大会》
会议日期：2017年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
关 键 词：Hepatitis B Liver Sinusoidal Endothelial Cells T cell immunity
摘 要：Backgrounds & Aims:Liver sinusoidal endothelial cells(LSECs) play a crucial role in maintaining the homeostasis of the hepatic microenvironment through induction of Ag-specific T cell *** has been shown that some viruses infection or TLRs stimulation can induce the maturation of LSECs,which reverts their suppressive properties to induce T cell ***,little is known about whether and how the function of LSECs in regulating T cell immunity is regulated during HBV ***,we investigated the function and mechanism of LSECs regulating CD8 T cell immunity during HBV ***:Mice were hydrodynamic injected with pAAV/HBV1.2 or pSM2,and their LSECs were freshly isolated at different time points post injection and co-cultured with polyclonal TCR-activated T *** freshly isolated from naive mice were stimulated by recombinant HBV antigen protein and co-cultured with polyclonal TCR-activated T *** phenotype and function of LSECs were characterized by flow cytometry,quantitative PCR,and functional ***:Compared to LSECs of naive mice or HI control mice,LSECs separated from HBV HI mice at 14 dpi reverted their suppressive properties to enhance T cell *** analysis demonstrated HBeAg stimulation could induce the maturation of naive LSECs and enable them to promote IFN-γ production by TCR-activated CD8 T *** separated from HBeAg-deficiency HBV HI mice were less capable of promoting T cell immunity compared to those separated from wild type HBV HI *** contrast,replenishing HBeAg expression in HBeAg-deficiency HBV HI mice resulted in better LSEC activation and led to accelerated HBV *** LSECs separated from HBV HI mice and HBeAg stimulated LSECs showed increased TNF-α ***:HBV replication can revert LSECs suppressive properties to promote T cell immunity,which is probably mediated by HBeAg induced LSECs TNF-α production.