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Sensitivity.and Specificity.of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep

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Advances in Microbiology 2016年第2期6卷 98-103页

作者： Waffa A. Ahmed Shaimaa A. Majeed Ameer H. Abdul Ameer Nawal D. Mahmmod Nameer I. Saeed luma. y. hanaa Zoonosis Diseases Unit College of Veterinary Medicine Baghdad University Baghdad Iraq Ministery.of Agriculture Agriculture Research Office Baghdad Iraq Vet Research & Laboratories Department Baghdad Iraq

Brucellosis is a zoonosis found throughout the world with major implications both in the field of public health and farming economy. It is most often caused by.Brucella abortus and Brucella melitensis. This study.aimed for evaluation of the sensitivity.and specificity.of immunochromatographic assay.(ICA) and comparing the results with those obtained from Rose Bengal Plate Test (RBPT) and in direct Enzy.e Linked Immunsorbent Assay.(ELISA) for serodiagnosis of sheep and goat brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were examined, antibodies against Brucella were detected in ram 8 (38.09%), 6 (28.57%) and 16 (76.19%) in ram samples, whereas they.were detected in 3 (15%), 1 (5%) and 12 (60%) serum sample of goats males by.RBPT, (ICA) and iELISA respectively. The kappa value between iELISA and ICA was -0.08 and that between RBPT and ELISA was -0.01 in ram and the kappa value between iELISA and ICA was -0.06 and that between RBPT and ELISA was -0.14 in male goats. The sensitivity.for RBPT and ICA were 37.5% and 25% while specificity.was 60% and 60% in ram;whereas, in male goats, sensitivity.for RBPT was 8.33% and ICA was 8.33% while specificity.was 75% and 100% respectively. It can be concluded that the developed ICA is immunodiagnostic assay. and it is rapid, non-expensive, economical and suitable for large-scale screening in developing countries and rural areas.

关键词： Brucellosis RBPT ICA iELISA Sheep Goat

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