Sensitivity and Specificity of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep
Sensitivity and Specificity of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep作者机构：Zoonosis Diseases Unit College of Veterinary Medicine Baghdad University Baghdad Iraq Ministery of Agriculture Agriculture Research Office Baghdad Iraq Vet Research & Laboratories Department Baghdad Iraq
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2016年第6卷第2期
页 面：98-103页
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
主 题：Brucellosis RBPT ICA iELISA Sheep Goat
摘 要：Brucellosis is a zoonosis found throughout the world with major implications both in the field of public health and farming economy. It is most often caused by Brucella abortus and Brucella melitensis. This study aimed for evaluation of the sensitivity and specificity of immunochromatographic assay (ICA) and comparing the results with those obtained from Rose Bengal Plate Test (RBPT) and in direct Enzyme Linked Immunsorbent Assay (ELISA) for serodiagnosis of sheep and goat brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were examined, antibodies against Brucella were detected in ram 8 (38.09%), 6 (28.57%) and 16 (76.19%) in ram samples, whereas they were detected in 3 (15%), 1 (5%) and 12 (60%) serum sample of goats males by RBPT, (ICA) and iELISA respectively. The kappa value between iELISA and ICA was -0.08 and that between RBPT and ELISA was -0.01 in ram and the kappa value between iELISA and ICA was -0.06 and that between RBPT and ELISA was -0.14 in male goats. The sensitivity for RBPT and ICA were 37.5% and 25% while specificity was 60% and 60% in ram;whereas, in male goats, sensitivity for RBPT was 8.33% and ICA was 8.33% while specificity was 75% and 100% respectively. It can be concluded that the developed ICA is immunodiagnostic assay, and it is rapid, non-expensive, economical and suitable for large-scale screening in developing countries and rural areas.
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