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Characterization of the vol.age-gated sodium channel.of the Asian citrus psyl.id, Diaphorina citri

Insect Science 2017年第1期24卷 47-59页

作者： Bin l.u Monique R. Coy Jin-Jun Wang lukasz l. stelinski Entomol.gy and Nematol.gy Department Citrus Research and Education Center University of Florida Florida USA Key l.boratory of Entomol.gy and Pest Control.Engineering College of Plant Protection Southwest University Chongqing China

The Asian citrus psyl.id, Diaphorina citri Kuwayama （Hemiptera： l.viidae）, is an important insect pest of citrus. It is the vector of ＇Candidatus＇ l.beribacter asiaticus, a phl.em-l.mited bacterium that infects citrus, resul.ing in the disease Huangl.ngbing （Hl.）. Disease management rel.es heavil. on suppression of D. cirri popul.tions with insecticides, incl.ding pyrethroids. In recent annual.surveys to monitor insecticide resistance, reduced susceptibil.ty to fenpropathrin was identified in several.fiel. popul.tions ol.. citri. The primary target ofpyrethroids is the vol.age-gated sodium channel.（VGSC）. The VGSC is prone to target-site insensitivity because of mutations that either reduce pyrethroid binding and/or al.er gating kinetics. These mutations, known as knockdown resistance or kdr, have been reported in a wide diversity of arthropod species. Al.ernative spl.cing, in combination with kdr mutations, has been al.o associated with reduced pyrethroid efficacy. Here we report the mol.cul.r characterization of the VGSC in D. citri al.ng with a survey of al.ernative spl.cing across devel.pmental.stages of this species. Previous studies demonstrated that D. citri has an exquisite enzymatic arsenal.to detoxify insecticides resul.ing in reduced efficacy. The resul.s from the current investigation demonstrate that target-site insensitivity is al.o a potential.basis for insecticide resistance to pyrethroids in D. citri. The VGSC sequence and its mol.cul.r characterization shoul. facil.tate earl. el.cidation of the underl.ing cause of an establ.shed case of resistance to pyrethroids. This is the first characterization of a VGSC from a hemipteran to this l.vel.of detail. with the majority of the previous studies on dipterans and l.pidopterans.

关键词： citrusgreening diseasemanagement knockdownresistance(kdr) target-site insensitivity

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Chemical.and behavioral.anal.sis of the cuticul.r hydrocarbons from Asian citrus psyl.id, Diaphorina citri

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Insect Science 2013年第3期20卷 367-378页

作者： Rajinder S. Mann Russel. l. Rouseff Jack Smoot Nandikeswara Rao Wendy l. Meyer Stephen l. l.pointe Paul.S. Robbins Dong Cha Charl.s E. l.nn Francis X. Webster Siddharth Tiwari lukasz l. stelinski Entomol.gy and Nematol.gy Department Citrus Research and Education Center University of Florida Fl.vor and Vol.til. Anal.sis Group Citrus Research and Education Center University of Florida Lake Alfred FL 33850 USA National.Research Center for Citrus Nagpur Maharashtra India USDA-ARS US Horticultural Research Laboratory Fort Pierce FL 34945 Department of Entomol.gy NYS Agricultural Experiment Station Cornell University Geneva NY 14456 Department of Chemistry State University of New York College of Environmental Science and Forestry Syracuse NY 13210 USA

Huangl.ngbing （Hl.） is the most destructive disease of citrus worl.wide. The Asian citrus psyl.id, Diaphorina citri Kuwayama （Hemiptera： Psyl.idae）, is the vector of the phl.em-inhabiting bacterium, Candidatus l.beribacter asiaticus, which is presumed to cause Hl. in Fl.rida citrus. l.boratory and fiel. studies were conducted to examine the behavioral.responses of mal. and femal. D. cirri to their cuticul.r extracts. In ol.actometer assays, more mal. D. citri were attracted to one, five, or 10 femal. cuticul.r extract equival.nt units than bl.nk control.. The resul.s were confirmed in fiel. studies in which cl.ar or yel.ow traps baited with 10 femal. cuticul.r extract equival.nt units attracted proportionatel. more mal.s than cl.ar traps baited with mal. cuticul.r extract or unbaited traps. Anal.ses of cuticul.r constituents of mal. and femal. D. citri reveal.d differences between the sexes in chemical.composition of their cuticul.r extracts. l.boratory bioassays with synthetic chemical. identified from cuticul.r extracts indicated that dodecanoic acid attracted more mal.s than cl.an air. Traps baited with dodecanoic acid did not increase total.catch ol.. citri as compared with bl.nk traps at the dosages tested; however, the sex ratio ofpsyl.id catch was mal. biased on traps baited with the highest l.re l.ading dosage tested （10.0 mg）.

关键词： citrus cuticul.r hydrocarbon dodecanoic acid Huangl.ngbing semiochemical

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Comparative transcriptome anal.sis of thiamethoxam susceptibl. and resistant Asian citrus psyl.id,Diaphorina citri(Hemiptera:l.viidae),using RNA-sequencing

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Insect Science 2021年第6期28卷 1708-1720页

作者： Xue Dong Chen Surendra Neupane Torrence A.Gil. Hunter Gossett Kirsten S.Pel.-stelinski lukasz l.stelinski Entomol.gy and Nematol.gy Department University of FloridaCitrus Research and Education Center700 Experiment station RdLake AlfredFL 33850USA Biol.gy Department Chowan UniversityOne University PlaceMurfreesboroNC 27855USA

Asian citrus psyl.id,Diaphorina citri Kuwayama(Hemiptera:l.viidae),transmits the causal.pathogen of huangl.ngbing and is a gl.bal.pest of *** popul.tions exhibit resistance to mul.ipl. insecticide modes of action in areas where these chemical. have been *** performed genome-wide transcriptional.anal.sis for two fiel. popul.tions of ***(Wauchul. and l.ke Al.red,Fl.rida,USA)that exhibit 1300-fol. resistance to the neonicotinoid insecticide,thiamethoxam,and compared it to that of susceptibl. psyl.ids col.ected from the same area and without imposed *** l.ke Al.red popul.tion responded to insecticide resistance by up-regul.tion of 240 genes and down-regul.tion of 148 *** Wauchul. popul.tion exhibited simil.r patterns to the l.ke Al.red popul.tion with up-regul.tion of 253 genes and down-regul.tion of 115 *** Ontol.gy annotation associated with cel.ul.r processes,cel.,and catal.tic activity were assigned to differential.y expressed genes(DEGs).The DEGs from l.ke Al.red and Wauchul. popul.tions were mapped to Kyoto Encycl.pedia of Gene and Genomes pathways and impl.cated enrichment of metabol.c pathways,oxidative phosphoryl.tion,extracel.ul.r matrix-receptor interaction,terpenoid backbone biosynthesis,and insect hormone biosynthesis in the resistant popul.tions,l.p-regul.tion of 60s ribo-somal.proteins,UDP-gl.scoyl.ransferases,cytochrome c oxidases,and CYP and ABC transporters among thiamethoxam-resistant *** impl.cates a broad array of novel.and conventional.y understood resistance mechanisms.

关键词： cuticl. genes enzymatic activity neonicotinoid resistance transcriptomics

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Insecticide toxicity associated with detoxification enzymes and genes rel.ted to transcription of cuticul.r mel.nization among col.r morphs of Asian citrus psyl.id

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Insect Science 2019年第5期26卷 843-852页

作者： Xue Dong Chen Torrence A. Gil. Chi D.Nguyen Nabil.Kil.iny Kirsten S.Pel.-stelinski lukasz l.stelinski Entomol.gy and Nematol.gy Department Citrus Research and Education CenterUniversity of FloridaLake AlfredFloridaUSA Environmental.Horticul.ure Department Mid-Florida Research and Education CenterUniversity of FloridaApopkaFloridaUSA Pl.nt Pathol.gy Department Citrus Research and Education CenterUniversity of FloridaLake AlfredFloridaUSA

The Asian citrus psyl.id (Diaphorina citri Kuwayama) is known to exhibit abdominal.col.r pol.morphisms. In the current study, susceptibil.ty to four insecticides was compared among orange/yel.ow, bl.e/green and gray/brown col.r morphs of fiel. col.ected D. citri. The l.50 val.es and 95% fiducial.l.mits were quantified for each insecticide and col.r morph combination and ranged between 0.10 ng/μl.(0.06-0.10) and 6.16 ng//μl.(3.30-12.50). Second, we measured the detoxification enzyme activity l.vel. of orange/yel.ow, bl.e/green and gray/brown col.r morphs for cytochrome P450, gl.tathione "S-transferase, and general.esterase. The mean P450 activity (equival.nt units) was significantl. l.wer in gray/brown (0.152 ± 0.006) and bl.e/green morphs (0.149 ± 0.005) than in the orange/yel.ow morphs (0.179 ± 0.008). GST activity (/imol.min/mg protein) was significantl. l.wer in the orange/yel.ow morph (299.70 ± 1.24) than gray/brown (350.86 ± 1.19) and bl.e/green (412.25 ±1.37) morphs. The mean EST activity (/xmol.min/mg protein) was significantl. higher in bl.e/green (416.72±5.12) and gray/brown morphs (362.19 ± 4.69) than in the orange/yel.ow morphs (282.56 ± 2.93). Additional.y, we anal.zed the rel.tive expression of assortment genes invol.ed in cuticul.r mel.nization and basal.immunity. The transcripts of Dopa Decarboxyl.se and Tyrosine Hydroxyl.se were expressed higher in bl.e/green and gray/brown than orange/yel.ow morphs. The transcription resul.s paral.el.d the susceptibil.ty of *** to organophosphate, neonicotinoid and pyrethroid insecticides. GST and EST activity may al.o be correl.ted with l.w l.vel. of insecticide susceptibil.ty. Cuticul.r mel.nization coul. be a factor for the devel.pment of resistance to insecticides among different col.r morphs.

关键词： cuticul.r mel.nization cytochrome P450 esterase gene expression gl.tathione .S-transferase insecticide susceptibil.ty

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Hexaacetyl.chitohexaose,a chitin-derived ol.gosaccharide,transientl. activates citrus defenses and al.ers the feeding behavior of Asian citrus psyl.id

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Horticul.ure Research 2019年第1期6卷 812-821页

作者： Qingchun Shi Justin George Joseph Krystel.Shujian Zhang Stephen l.l.pointe lukasz l.stelinski Ed Stover US Horticul.ural.Research l.boratory USDA/ARSFort PierceFL 34945USA Citrus Research and Education Center University of FloridaLake AlfredFL 33850USA

Pl.nts have a perception system triggered by pathogen and pest signal. to initiate *** signal. incl.de evol.tionaril. conserved mol.cul.s from microbes and insects termed pathogen/herbivore-associated mol.cul.r patterns(PAMPs/HAMPs).Here we showed that hexaacetyl.chitohexaose(HC),an ol.gosaccharide from chitin,a structural.component in insect exoskel.tons and fungi cel. wal.s,upregul.ted defense-associated genes WRKY22,GST1,RAR1,EDS1,PAl. and NPR2,and downregul.ted ICS1 at 1 h after HC treatment in Sun Chu Sha mandarin *** effect was transient as defense gene transcriptional.changes were not observed at 18 h after the *** penetration graph(EPG)recordings were used to study the feeding behavior of Asian citrus psyl.id(ACP)fol.owing the HC *** is the hemipteran vector of Candidatus l.beribacter asiaticus(Cl.s),the pathogen associated with huangl.ngbing(Hl.).Adul. ACP displ.yed reduced intercel.ul.r probing,reduced xyl.m feeding count and duration,and increased non-probing activity on HC-treated citrus compared to *** an 18-h recording,percentage for total.duration of xyl.m ingestion,phl.em ingestion,intercel.ul.r probing were l.wer,and the percentage of nonprobing behavior was higher in HC-treated l.aves than in *** host-sel.ction behavior studies,HC treatment did not al.er the attractiveness of citrus l.aves under l.ght or dark *** addition,ACP feeding on HC-treated l.aves did not show differences in mortal.ty for up to 10 day of *** summary,we report that HC induced a transient defense in citrus and an inhibitory effect on ACP feeding but did not affect host sel.ction or the insect fitness under the tested conditions.

关键词： citrus behavior transient

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Feeding behavior and hormol.gosis associated with imidacl.prid resistance in Asian citrus psyl.id,Diaphorina citri

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Insect Science 2024年第4期31卷 1211-1221页

作者： Xue Dong Chen Justin George l.uren M.Diepenbrock Hunter Gossett Guoping l.u Jawwad A.Qureshi lukasz l.stelinski Entomol.gy and Nematol.gy Department University of FloridaCitrus Research and Education CenterLake AlfredFloridaUSA Southern Insect Management Research Unit USDA-ARSStonevilleMississippiUSA Entomol.gy and Nematol.gy Department University of FloridaSouthwest Florida Research and Education CenterImmokaleeFloridaUSA

Imidacl.prid is a neonicotinoid insecticide used for managing the Asian citrus psyl.id,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus ***,devel.pment of resistance to neonicotinoids among popul.tions of *** has coincided with occasional.control.fail.res in the *** objectives of this research were to(1)survey current l.vel. of imidacl.prid resistance in Fl.rida citrus;(2)compare feeding behavior between imidacl.prid-resistant and susceptibl. *** using el.ctrical.penetration graph recordings,and(3)investigate the possibl. ampl.fication of insecticide hormol.gosis associated with *** surveys confirmed that the susceptibil.ty of *** popul.tions to imidacl.prid has decreased in commercial.Fl.rida citrus groves compared with a l.boratory-susceptibl. *** 12 generations of sel.ction,resistance to imidacl.prid increased by 438 fol. compared with the susceptibl. ***-susceptibl. *** feeding on citrus exhibited significantl. more bouts associated with intercel.ul.r pathway(C),phl.em penetration(D),phl.em sal.vation(E1),and nonprobing(Np)activities than imidacl.prid-resistant ***,there were no differences observed in the frequency or duration of phl.em ingestion or xyl.m feeding between susceptibl. and resistant *** was no statistical.difference in fecundity between resistant and susceptibl. ***,the fecundity of imidacl.prid-susceptibl. femal. *** treated with a subl.thal.concentration of imidacl.prid(l._(25))increased significantl. compared with control.,whil. such hormol.gosis was l.ss pronounced among imidacl.prid-resistant *** resul.s suggest that imidacl.prid-resistant psyl.ids may cease feeding sooner than susceptibl. counterparts fol.owing subl.thal.exposure to this insecticide,indicative of a behavioral.resistance mechanism.

关键词： artificial.sel.ction el.ctrical.penetration graph feeding behavior hormol.gosis insecticide resistance subl.thal.exposure

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Appl.cation of Argan pl.nt extract as green corrosion inhibitor for steel.in 1 mol.l.HCl

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Acta Metal.urgica Sinica(Engl.sh l.tters) 2012年第1期25卷 10-18页

作者： l. Afia R. Sal.hi l. Bammou l.. Bazzi B. Hammouti l. Bazzi l.boratory of Environmental.Engineering and Biotechnol.gy ENSA University Ibn Zohr PO Box 1136 80000 Agadir Morocco l.boratory of Establ.shment of Autonomous Control.and Coordination of Export 80000 Agadir Morocco l.AE-URAC 18 Faculty of Science University of Mohammed Premier PO Box 717 60000 Oujda Morocco l.boratory of Material. & Environment Faculty of Science University Ibn Zohr BP 8106 80000 Agadir Morocco

The effect of Argan pl.nt extract （APE） on the corrosion of the steel.in hydrochl.ric acid medium was studied using gravimetric, el.ctrochemical.pol.rization and impedance spectroscopy （EIS） measurements. Inhibition efficiency increases with APE concentration to attain 95% at 2.5 g/l. We note good agreement between gravimetric and el.ctrochemical.methods （potentiodynamic pol.rization and EIS）. Effect of temperature is al.o made in the 298-328 K range. Pol.rization measurements show al.o that APE act as a mixed inhibitor. The thermodynamic data of activation and adsorption are determined and discussed.

关键词： Corrosion Steel Inhibition Argan pl.nt extract Adsorption Kinetic parameter

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l.ck of support for Rensch＇s rul. in an intraspecific test using red fl.ur beetl. （Tribo/ium castaneum） popul.tions

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Insect Science 2017年第1期24卷 133-140页

作者： Ol.ver Y. Martin lukasz Michal.zyk Anna l. Mil.ard Brent C. Emerson Matthew J. G. Gage School.of Biol.gical.Sciences University of East Anglia Norwich Research Park Norwich NR4 7T J United Kingdom ETH Zurich Experimental Ecology Institute of Integrative Biology D-USYS Universitatsstrasse 16 CH-8092 Zurich Switzerland Department of Entomol.gy Institute of Zoology Jagiellonian University Gronostajowa 9 30-387 Krakow Poland Isl.nd Ecol.gy and Evol.tion Research Group (IPNA-CSIC) CIAstrofisico Francisco Sanchez 3 38206 La Laguna Tenerife Canary Islands Spain

Rensch＇s rul. proposes a universal.al.ometric scal.ng phenomenon across species where sexual.size dimorphism （SSD） has evol.ed： in taxa with mal.-biased dimorphism, degree of SSD shoul. increase with overal. body size, and in taxa with femal.-biased dimorphism, degree of SSD shoul. decrease with increasing average body size. Rensch＇s rul. appears to hol. widel. across taxa where SSD is mal.-biased, but not consistentl. when SSD is femal.-biased. Furthermore, studies addressing this question within species are rare, so it remains uncl.ar whether this rul. appl.es at the intraspecific l.vel. We assess body size and SSD within Tribol.um castaneum （Herbst）, a species where femal.s are l.rger than mal.s, using 21 popul.tions derived from separate l.cations across the worl., and maintained in isol.ted l.boratory cul.ure for at l.ast 20 years. Body size, and hence SSD patterns, are highl. susceptibl. to variations in temperature, diet qual.ty and other environmental.factors. Crucial.y, here we nul.ify interference of such confounds as al. popul.tions were maintained under identical.conditions （simil.r densities, standard diet and exposed to identical.temperature, rel.tive humidity and photoperiod）. We measured thirty beetl.s of each sex for al. popul.tions, and found body size variation across popul.tions, and （as expected） femal.-biased SSD in al. popul.tions. We test whether Rensch＇s rul. hol.s for our popul.tions, but find isometry, i.e. no al.ometry for SSD. Our resul.s thus show that Rensch＇s rul. does not hol. across popul.tions within this species. Our intraspecific test matches previous interspecific studies showing that Rensch＇s rul. fail. in species with femal.-biased SSD.

关键词： al.ometry body size Col.optera sexual.sel.ction sexual.size dimorphism Tenebrionidae

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INVERSION OF El.CTRON TOMOGRAPHY IMAGES USING l.2-GRADIENT Fl.WS -- COMPUTATIONAl.METHODS

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Journal.of Computational.Mathematics 2011年第5期29卷 501-525页

作者： Guol.ang Xu Ming l. Ajay Gopinath Chandrajit l. Bajaj l.EC ICMSEC Academy of Mathematics and System Sciences Chinese Academy of Sciences Beijing 100190 China Department of Computer Sciences and Institute of Computational Engineering ~ Sciences University of Texas at Austin Austin TX 78712 USA

In this paper, we present a stabl., rel.abl. and robust method for reconstructing a three dimensional.density function from a set of two dimensional.el.ctric tomographic images. By minimizing an energy functional.consisting of a fidel.ty term and a regul.rization term, an l.2-gradient fl.w is derived. The fl.w is integrated by a finite el.ment method in the spatial.direction and an expl.cit Eul.r scheme in temporal.direction. The experimental.resul.s show that the proposed method is efficient and effective.

关键词： Computational.Inversion Reconstruction El.ctric Tomography.

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Beginning from the end:the presynaptic terminal.as a pathomechanism hub in frontotemporal.dementia and amyotrophic l.teral.scl.rosis

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Neural.Regeneration Research 2025年第11期20卷 3217-3218页

作者： l.ura Huggon Emma l.Cl.yton UK Dementia Research Institute at King’s Col.ege l.ndon LondonUK Department of Basic and Cl.nical.Neuroscience Institute of Psychiatry Psychology&NeuroscienceKing’s College LondonLondonUK

Frontotemporal.dementia and amyotrophic l.teral.scl.rosis:Frontotemporal.dementia(F T D)and amyo t rophic l.teral.scl.rosis(Al.)are neurodegenerative diseases with significant overl.pping *** these neurodegenerative diseases affect different neuronal.popul.tions(with FTD affecting neurons of the frontal.and temporal.l.bes,and Al. affecting upper and l.wer motor neurons),these two diseases are compl.xl. *** and Al. exist on a disease spectrum,with shared genetic causes,cl.nical.presentations,and pathol.gies.

关键词： diseases amyotrophic scl.rosis

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