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An inter-laboratory study of DNA-based identity,parentage and species testing in animal forensic genetics

Forensic Sciences Research 2022年第4期7卷 708-713页

作者： Sreetharan Kanthaswamy Torsten Brendel luis cancela Denise A.Andrade de Oliveira Bertram Brenig Carmen Cons Julian A.Crespi Markéta Dajbychová Andreas Feldl Tomohito Itoh Vincenzo Landi Amparo Martinez Malgorzata Natonek-Wisniewska Robert F.Oldt Anna Radko Oscar Ramírez Clementina Rodellar Manuel Ruiz-Girón David Schikorski María Elena Turba Guillermo Giovambatista School of Mathematics and Natural Sciences Arizona State University(ASU)at the West CampusGlendaleAZUSA California National Primate Research Center University of CaliforniaDavisCAUSA Evolutionary Biology Graduate Program School of Life SciencesArizona State UniversityTempeAZUSA Eurofins Genomics Europe Applied Genomics GmbH EbersbergGermany IDENTITAS MontevideoUruguay Laboratório de Genética Escola de Veterinária da UFMGBelo HorizonteBrazil Institute of Veterinary Medicine University of GoettingenGoettingenGermany Facultad de Veterinaria Laboratorio de Genética Bioquímica(LAGENBIO)Instituto Agroalimentario de Aragón-IA2(Universidad de Zaragoza-CITA)ZaragozaSpain Facultad de Ciencias Veterinarias UNLP IGEVET-Instituto de Genética Veterinaria(UNLP-CONICET LAPLATA)La PlataArgentina Genomia s.r.o. PlzeňCzech Republic Maebashi Institute of Animal Science Livestock Improvement Association of Japan(LIAJ)MaebashiJapan Department of Veterinary Medicine Animal Breeding and GeneticsUniversity of Bari“Aldo Moro”ValenzanoItaly Department of Genetics Faculty of Veterinary SciencesUniversity of CórdobaCórdobaSpain Department of Animal Molecular Biology National Research Institute of Animal ProductionBalicePoland Vetgenomics SL BarcelonaSpain Laboratorio de Biología Molecular y Genómica Hispalis Biolab S.L.U.SevilleSpain Laboratoire LABOFARM-GENINDEXE LOUDEACCedexFrance Genefast SRL ForlìItaly

The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability.Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output.The International Society for Animal Genetics(ISAG)administered animal forensic comparison tests(AFCTs)in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification,parentage and species determination services.The AFCTs revealed that analyses of low DNA template concentrations(≤300 pg/μL)constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results.Moreover,a lack of familiarity with species testing protocols,interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results.Several laboratories showed improvement in their genotyping accuracy over time.However,the use of forensically validated standards,such as a standard forensic short tandem repeat(STR)kit,preferably with an allelic ladder,and stricter guidelines for STR typing,may have prevented some common issues from occurring,such as genotyping inaccuracies,missing data,elevated stutter products and loading errors.The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other.Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number(LCN)analysis and species testing.Although this is the first time that the ISAG has conducted comparison tests for forensic testing,findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.

关键词： Forensic sciences forensic genetics comparison test forensic DNA analysis individual identification parentage test species test

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Extensive right coronary artery thrombosis in a patient with COVID-19:A case report

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World Journal of Clinical Cases 2022年第31期10卷 11508-11516页

作者： Clarissa Campo Dall’Orto Rubens Pierry Ferreira Lopes Mariana Torres cancela Ciria de Sales Padilha Gilvan Vilella Pinto Filho Marcos Raphael da Silva Therapy Center Brazilian Society of Health Support HospitalTeixeira de Freitas 45987-088BahiaBrazil Department of Cardiology Deputy Luis Eduardo Magalhães HospitalPorto Seguro 45810-000BahiaBrazil Intensive Care Unit Medical Assistance Extreme South HospitalEunápolis 45820-131BahiaBrazil

BACKGROUND Occurring in approximately 30%of hospitalized patients,cardiovascular complications that take place during the course of coronavirus disease 2019(COVID-19)have been shown to cause morbidity and mortality.This case is the first report of extensive right coronary artery(RCA)thrombosis that was evaluated by intracoronary imaging and intracoronary invasive physiology in a patient with COVID-19.CASE SUMMARY A 62-year-old woman presented with flu-like symptoms;ten days later,she presented with inferior ST-segment elevations,chest pain,dyspnea,nausea and vomiting.The patient was diagnosed with COVID-19 following a positive test result.Emergency angiography of the RCA and its branches indicated intraluminal filling defects,suggesting a thrombus.Intravascular ultrasound confirmed a subacute thrombus in the RCA,the right posterior descending branch and the right posterior ventricular(RPV)branch.There was also an acute thrombus in the RPV branch and atherosclerosis in the RCA.Dual antiplatelet/anticoagulation therapy was administered.After 7 d,angiography revealed complete disappearance of the thrombi.Optical coherence tomography confirmed this with the exception of a small thrombus in the RPV branch and atherosclerotic plaque in the RCA.The atherosclerotic RCA was measured using the resting fullcycle ratio,indicating no impairment to coronary physiology.The patient was discharged on the 11^(th) day of hospitalization and remained asymptomatic through the 6-mo follow-up.CONCLUSION This was the first report of RCA thrombosis in a patient with COVID-19.Dual antiplatelet/anticoagulation therapy was successful.

关键词： Acute coronary syndrome Coronary angiography COVID-19,Intravascular ultrasound Thrombosis Case report

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