检索结果-南通市图书馆

A multi-integrated approach on toxicity effects of engineered TiO2 nanoparticles

Frontiers of Environmental Science & Engineering 2015年第5期9卷 793-803页

作者： Ana PICADO Susana M. PAIXAO Liliana MOITA Luis silva Mario S. DINIZ Joana LOURENCO Isabel PERES Luisa CASTRO Jose Brito CORREIA Joana pereira Isabel FERREIRA Antonio Pedro Alves MATOS Pedro BARQUINHA Elsa MENDONCA LNEG-National Laboratory of Energy and Geology I.P. Lisbon 1649-038 Portugal REQUIMTE Chemistry Department Fine Chemistry and Biotechnology Center Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal IMAR-Ocean Institute Department of Sciences and Environmental Engineering Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal CENIMAT/I3N and Department of Materials Science Faculty of Sciences and Technology New University of Lisbon Caparica 2829-516 Portugal Pathological Anatomy Curry Cabml Hospital Lisbon 1069-166 Portugal CESAM Faculty of Sciences Lisbon University Lisbon 1749-016 Portugal

The new properties of engineered nanoparti- cles drive the need for new knowledge on the safety, fate, behavior and biologic effects of these particles on organisms and ecosystems. Titanium dioxide nanoparticles have been used extensively for a wide range of applica- tions, e.g, self-cleaning surface coatings, solar cells, water treatment agents, topical sunscreens. Within this scenario increased environmental exposure can be expected but data on the ecotoxicological evaluation ofnanoparticles are still scarce. The main purpose of this work was the evaluation of effects of TiO2 nanoparticles in several organisms, covering different trophic levels, using a battery of aquatic assays. Using fish as a vertebrate model organism tissue histological and ultrastructural observations and the stress enzyme activity were also studied. TiO2 nanoparticles （Aeroxide~ P25）, two phase composition ofanatase （65%） and rutile （35%） with an average particle size value of 27.64-11 nm were used. Results on the ECs0 for the tested aquatic organisms showed toxicity for the bacteria, the algae and the crustacean, being the algae the most sensitive tested organism. The aquatic plant Lemna minor showed no effect on growth. The fish Carassius auratus showed no effect on a 21 day survival test, though at a biochemical level the cytosolic Glutathione-S-Transferase total activity, in intestines, showed a general significant decrease （p〈0.05） after 14 days of exposure for all tested concentrations. The presence of TiO2 nanoparticles aggregates were observed in the intestine lumen but their internalization by intestine cells could not be confirmed.

关键词： ecotoxicity enzymatic analysis histology,transmission electron microscopy (TEM) TiO2-nanoparti-cles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy

引用

Advances in Bioscience and Biotechnology 2014年第11期5卷 903-914页

作者： dayse Drielly Souza Santana-Vieira Debora Marcondes Bastos pereira Milori Paulino Ribeiro Villas Boas Marina Franca e silva Magno Guimaraes Santos Fernanda Amato Gaiotto Walter dos Santos Soares Filho Abelmon da silva Gesteira Embrapa Cassava and Fruits Cruz das AlmasBrazil Department of Biological Sciences State University of Santa CruzIlheusBrazil Embrapa Instrumentation Sao CarlosBrazil

The differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties.

关键词： Differentiation of Citrus Genotypes Sunki LIFS Molecular Markers PLSR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Characterization and Cytotoxic Activity of Antarctic Macroalgae Extracts against Colorectal Cancer

引用

Advances in Biological Chemistry 2019年第5期9卷 167-177页

作者： Rafaele Frassini Yeslei Paulino da silva Sidnei Moura Leonardo Zambotti Villela Aline Paternostro Martins Pio Colepicolo Mutue Toyota Fujii Nair S.Yokoya Claudio Martin pereira de pereira Vagner Reinaldo Zingalli Bueno pereira Joao Antonio Pegas Henriques Mariana Roesch-Ely Laboratory of Genomics Proteomics and DNA RepairUniversity of Caxias do SulCaxias do SulRSBrazil Laboratory of Biosynthetic and Natural Products of Caxias do Sul Caxias do SulRSBrazil Laboratory of Biochemistry and Molecular Biology of Algae University of Sao PauloSao PauloSPBrazil Phycologycal Research Institute of BotanySao PauloSPBrazil Laboratory of Lipidomics and Biorganic—LLipidomicaBio Federal University of PelotasPelotasRSBrazil General Hospital University of Caxias do SulCaxias do SulRSBrazil

Background/Aim: Antarctic seaweeds are considered a promising source of compounds with anticancer activity. Colorectal cancer (CRC) is one of the most incident cancers with high mortality rates worldwide. This work aimed to characterize chemically extracts of the Antarctic macroalgae Iridaea cordata, Cystosphaera jacquinotii and Desmarestia anceps and to evaluate the cytotoxic effects against human colon cancer HCT 116 cell line. Materials and Methods: The extracts were obtained by depletion using an ultrasound probe and were identified by High-Performance Liquid Chromatography (HPLC) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). Cell viability was determined by MTT assay. Results: Hexanic and chloroform extracts of the I. cordata and the hexanic, chloroform and methanolic extracts of D. anceps were able to inhibit growth of colorectal cancer cells in the three different incubation times (24, 48 and 72 h). Through GC analysis, 01 compounds were identified in the hexane extract and 02 compounds in the chloroform extract of the algae I. cordata. The hexane extract of D. anceps macroalgae presented 5 compounds, chloroform extract 10 and methanolic extract 3 respectively, with special highlight to fucosterol. Carotenoid analysis by HPLC identified β-carotene in all species, while zeaxanthin was present in the spectrum of I. cordata and C. jacquinotii. Fucoxanthin and violaxanthin were confirmed in the brown seaweeds C. jacquinotii and D. anceps. Conclusion: Extracts of macroalgae I. Cordata and D. anceps may be a source of therapeutic agents against CRC.

关键词： Antarctic Seaweeds Antitumor Activity Colorectal Cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oily Water Treatment by Ceramic Membrane: Modeling and Simulation

引用

Open Journal of Fluid Dynamics 2021年第1期11卷 1-19页

作者： Adriana Barbosa da Costa pereira Hortência Luma Fernandes Magalhães Leonardo pereira de Lucena silva Cristiane Arcoverde Passos Ricardo Soares Gomez Balbina Raquel de Brito Correia Severino Rodrigues de Farias Neto Antonio Gilson Barbosa de Lima Department of Chemical Engineering Federal University of Campina Grande Campina Grande Brazil Department of Industry Federal Institute of Paraiba Cajazeiras Brazil Department of Chemical Engineering Federal University of Paraná Curitiba Brazil Department of Mechanical Engineering Federal University of Campina Grande Campina Grande Brazil

The separation process of oily water using membranes has attracted the attention of researchers and engineers. The greater problem in the use of membrane separation process is the reduction in permeate flux due to clogged pores by oil deposition inside the membrane or by the effect of the concentration polarization. For this purpose, a theoretical study of a water/oil separation module was performed. This device consists of a tubular ceramic membrane provided with a rectangular inlet section. Numerical simulations were performed using Ansys CFX software to solve the mass and momentum conservation equations in the fluid and porous domains. Here was adopted the RNG k-ε turbulence model. The effect of the membrane porosity and the inlet velocity of the fluid mixture on the two-phase flow behavior inside the separation module were evaluated. Results of the volumetric fraction, velocity and pressure fields of the oil and water phases are presented and analyzed. The results indicate a higher oil concentration within the membrane for the cases of higher porosity, and that the inlet fluid mixture velocity does not substantially affect the velocity profile within the separation module. It is found that the maximum separation efficiency of the module was obtained with feed velocity of 40 m/s and membrane porosity of 0.44.

关键词： Ceramic Membranes Separation Process Computational Fluid Dynamics CFX

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Functional biomarker signatures of circulating T-cells and its association with distinct clinical status of leprosy patients and their respective household contacts

引用

Infectious Diseases of Poverty 2020年第6期9卷 5-15页

作者： Pedro Henrique Ferreira Marçal Rafael silva Gama Lorena Bruna pereira de Oliveira Olindo Assis Martins-Filho Roberta Olmo Pinheiro Euzenir Nunes Sarno Milton Ozório Moraes Lucia Alves de Oliveira Fraga Universidade Vale do Rio Doce–Univale Governador ValadaresMGBrazil Grupo Integrado de Pesquisas em Biomarcadores Institute Rene RachouFIOCRUZ-MinasBelo HorizonteMGBrazil Laboratorio de Hanseniase Instituto Oswaldo CruzFundagao Oswaldo Cruz-FIOCRUZ-RJRio de JaneiroRJBrazil Nucleo de Pesquisa em Hansenologia Universidade Federal de Juiz de ForaInstituto de Ciencias da VidaCam pus Governador ValadaresRua Sao Paulo582-CentroGovernador ValadaresMG 30190-002Brazil

Background: Leprosy is a chronic infectious disease classified into two subgroups for therapeutic purposes:paucibacillary(PB)and multibacillary(MB),closely related to the host immune *** this context it is noteworthy looking for immunological biomarkers applicable as complementary diagnostic tools as well as a laboratorial strategy to follow-up leprosy household ***:: The cross-sectional study enrolled 49 participants,including 19 patients and 30 healthy *** blood mononuclear cells(PBMC)were isolated and incubated in the presence of Mycobacterium leprae *** cells were prepared for surface(CD4+and CD8+)and intracytoplasmic cytokine staining(IFN-γ,IL-4 and IL-10).Multiple comparisons amongst groups were carried out by ANOVA,Kruskal–Wallis,Student T or Mann–Whitney *** analysis of categorical variables was performed by *** biomarker signature analysis was conducted using the global median values for each biomarker index as the cut-off edge to identify the proportion of subjects with high biomarker ***: The cytokine signature analysis demonstrated that leprosy patients presented a polyfunctional profile of T-cells subsets,with increased frequency of IFN-γ+T-cell subsets along with IL-10+and IL-4+from CD4+T-cells,as compared to health Controls(Venn diagram report).Moreover,statistical analysis was carried out using parametric or non-parametric variance analysis followed by pairwise multiple comparisons,according to the data normality distribution.L(PB)displayed a polyfunctional profile characterized by enhanced percentage of IFN-γ+,IL-10+and IL-4+produced by most T-cell subsets,as compared to L(MB)that presented a more restricted cytokine functional profile mediated by IL-10+and IL-4+T-cells with minor contribution of IFN-γproduced by CD4+*** was that HHC(MB)exhibited enhanced frequency of IFN-γ+T-cells,contrasting with HHC(PB)that presented a cytokine profile limited t

关键词： Leprosy Mycobacterium leprae Cytokines Household contacts

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Combined Use of NMR, LC-ESI-MS and Antifungal Tests for Rapid Detection of Bioactive Lipopeptides Produced by Bacillus

引用

Advances in Microbiology 2016年第10期6卷 788-796页

作者： Mara silva António pereira Dora Teixeira António Candeias Ana Teresa Caldeira Chemistry Department School of Science and Technology évora University évora Portugal HERCULES Laboratory évora University évora Portugal

The science and technology interact with the art in several ways. Biotechnological coupled with analytical approaches can play an important role in protecting and preserving cultural heritage for future generations. Many microorganisms influenced by environmental conditions are the main responsible for biological contamination in built heritage. Biocides based on chemical compounds have been used to mitigate this problem. Thus, it is vitally important to develop proper remediation actions based on environmentally innocuous alternative. Bacillus specie is emerging as an optimistic alternative for built heritage treatment due to their capacity to produce secondary metabolites with antagonistic activities against many fungal pathogens. Therefore, the intent of this work was to access a rapid evaluation of antifungal potential of bioactive metabolites produced by Bacillus strains and simultaneously their characterization using spectroscopic (NMR) and chromatographic techniques (LC-ESI-MS). The high antifungal activity obtained for Bacillus sp. active compounds produced in this study confirms the great potential to suppress biodeteriogenic fungi growth on historical artworks. Additionally, the proposed methodology allowed to access bioactive metabolites produced without need of the laborious total previous isolation and could be used as a viable alternative to be employed for screening and production of new green biocides.

关键词： Lipopeptides Bacillus sp. Biodegradation/Biodeterioration Natural Biocides Nuclear Magnetic Resonance Liquid Chromatography Coupled to Mass Spectrometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficiency in Teaching Speaker and Listener Repertoires:Comparing Three Instructional Sequences in Autistic Children

引用

Psychology Research 2023年第6期13卷 235-245页

作者： daniel Carvalho de Matos Pollianna Galvão Soares de Matos Neylla Cristina pereira Cordeiro Katiane Reis da silva Universidade Ceuma Brasil Universidade Federal do Maranhão and Instituto EvoluirSão Luís cityBrazil Universidade Ceuma and Instituto Evoluir São Luís cityBrazil

Previous studies have investigated the efficiency in teaching listener and speaker repertoires in children diagnosed with autism spectrum disorder(ASD).Some investigations focused on listener responding by function,feature,and class(LRFFC)and intraverbal by function,feature,and class(FFC).For some children,teaching intraverbal FFC was more efficient because it resulted in a better emergence effect of a related untaught repertoire(LRFFC).For other children,teaching LRFFC along with tacting pictures was more efficient,resulting in a better emergence effect of a related untaught repertoire(intraverbal FFC).In these cases,it is not clear whether the tact increased the efficiency of LRFFC training because a comparison with a condition in which tacts were not required was not *** investigation consisted of a replication with two children diagnosed with *** instructional sequences were compared:teaching LRFFC-probing intraverbal;teaching LRFFC+tacts-probing intraverbal;teaching intraverbal-probing *** a child,all sequences were equally efficient because all related untaught repertoires emerged without ***,the acquisition of intraverbals during training occurred with *** the case of the second child,the most efficient sequence consisted of teaching intraverbals,resulting in the emergence of LRFFC without *** both cases of teaching LRFFC,the emergence of related intraverbals was partial and acquisition of the trained repertoires occurred with *** case that did not demand tact responses was slightly more *** were discussed in the sense that the best instructional sequence may vary from learner to learner.

关键词： autism spectrum disorder listener repertoire speaker repertoire efficiency

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of Clinical and Environmental Isolates of <i>Cryptococcus neoformans</i>/<i>Cryptococcus gattii</i>Complex Maintained in Yeast Culture Collection in São Paulo, Brazil

引用

Open Journal of Epidemiology 2018年第2期8卷 76-92页

作者： Pedro Henrique Magalhã es Cardoso Bosco Christiano Maciel da silva Jairo Ivo dos Santos Rennan Luiz Oliveira dos Santos Diniz pereira Leite Jú nior Marcos Ereno Auler Luciana da silva Ruiz Eriques Gonç alves da silva Dé bora Moreira Carina Domaneschi Francisco de Assis Baroni Marcia de Souza Carvalho Melhem Marilena dos Anjos Martins Claudete Rodrigues Paula Faculty of Veterinary Medicine and Zootechny-FMVZ-USP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Faculty of Medicine/FMUSP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Federal University of Santa Catarina (UFSC) Florian&oacute polis Brazil Faculty of Dentistry University of S&#227 o Paulo (FOUSP) S&#227 o Paulo Brazil Faculty of Medicine Federal University of Mato Grosso (UFMT) Cuiab&aacute Brazil Faculty of Pharmacy University of the Western Center of Paran&aacute (UNICENTRO) Guarapuava Brazil Institute Adolfo Lutz (IAL) Bauru Brazil Federal Rural University of Rio de Janeiro (UFRRJ) Serop&eacute dica Brazil Institute Adolfo Lutz (IAL) S&#227 o Paulo Brazil

Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.

关键词： Cryptococcus Complex Genotyping Protease Phospholipase Etest Culture Collection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of applying a calcined kaolin-based particle film on the photosynthetic capacity and growth of young eucalyptus plants

引用

Journal of Forestry Research 2021年第6期32卷 2473-2484页

作者： Diego pereira Santos Mauriana da Rocha Sobrinho Maria de Fatima de Castro Oliveira Niedja Bezerra Costa Tiago Massi Ferraz Fabricio de Oliveira Reis Heder Braum Eliemar Campostrini Fabio Afonso Mazzei Moura de Assis Figueiredo Graduate Program in Agriculture and Environment State University of MaranhaoPraca Goncalves Diass/nCentroBalsasMA 65800-000Brazil Graduate Program in Agroecology State University of MaranhaoAv.Lourenco Vieira da Silva1000Jardim Sao CristovaoSao LuisMA 65055-310Brazil Graduate Program in Agronomy Campus SamambaiaEscola de AgronomiaFederal University of GoiasAv.Esperanca s/nGoianiaGO 74690-900Brazil State University of Maranhao Av.Lourenco Vieira da Silva1000Jardim Sao CristovaoSao LuisMA 65055-310Brazil Northern Rio de Janeiro State University Av.Alberto Lamego2000Campos dos GoytacazesRJ 28013-602Brazil

In scenarios of climatic change when increased global temperatures can be expected,it is essential to search for technologies that favor sapling survival and growth after planting and increase yield in the ***-based particle films(KBPF)have been applied as barriers against the deleterious effects of high levels of solar *** objective of the present study was to assess the effects of applying purified calcined kaolin-based particle film to young eucalyptus *** treatments were carried out:0%(just water),3%,5%,7%,and 10%calcined kaolin applied to the adaxial part of the leaves.A complete randomized block design was used with five treatments and ten *** plants were assessed for height,diameter at ground level,gas exchanges(net photosynthetic CO_(2) assimilation,stomatal conductance and transpiration),instantaneous(WUE)and intrinsic water use efficiency(IWUE),chlorophyll a fluorescence(maximum quantum yield of photosystem II(PSII)(Fv/Fm),concentration of active reaction centers in relation to the quantity of photons absorbed(RC/ABS)and the maximum ratio of quantum yields of photochemical and concurrent non-photochemical processes in PSII(Fv/F0),SPAD reading and leaf *** 3%KBPF concentration showed the best responses in biometric assessments 80 days after planting(daP)and were corroborated by the responses of the leaf,stem and shoot dry matter production as a *** leaf ontogeny assessments showed positive responses following KBPF application when considering leaf development,with 7%KBPF concentration resulting in the highest mean *** mean specific leaf mass had negative response to high KBPF *** 60 daP,the gas exchange variables during both assessment periods declined with an increase in KBPF *** differences as a result of KBPF applications were found only at the start of the assessments(34 daP)for both WUE and for ***‘greenness’(SPAD reading)at 47 days showed a quadratic

关键词： Ecophysiology Photoprotection Yield Silviculture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Assessment of Undergraduate Students in Use of Hypermedia Education on Peripheral Venous Punch

引用

Health 2015年第4期7卷 423-429页

作者： Natasha Marques Frota Lívia Moreira Barros Francisco Gilberto Fernandes pereira Máguida Gomes da silva Jennara Candido do Nascimento Monaliza Ribeiro Mariano Joselany áfio Caetano Department of Nursing Federal University of Ceará Fortaleza Brazil

This is a qualitative study that aims to describe the opinion of nursing students on the use of an educational hypermedia on Peripheral Venipuncture. We interviewed 16 nursing students of a private university on second semester of 2014. The interviews allowed the identification of units of meaning and categorization by content analysis and led to four categories: influence of hypermedia on learning, educational technology as support to teaching classroom, contribution of hypermedia for professional practice and access difficulties. Hypermedia showed positive results as most scholars reported that the material helped in learning and generated autonomy and interactivity in the moment of studying. However some reported difficulties in access hypermedia related mainly to the server used. So hypermedia proved to be a valuable tool to use, since it favored the learning and aroused the interest and curiosity of students.

关键词： Nursing Internet Educational Technology Educational Measurement Catherization Peripheral

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：