检索结果-南通市图书馆

Antibacterial activity of Bixa orellana L.(achiote) against Streptococcus mutans and Streptococcus sanguinis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 400-403页

作者： Dyanne Medina-Flores Gabriela Ulloa-Urizar Rosella Camere-Colarossi Stefany Caballero-García Frank Mayta-Tovalino Juana del Valle-mendoza School of Dentistry Faculty of Health SciencesPeruvian University of Applied Sciences-UPC Research and Innovation Center of the Faculty of Health Sciences Peruvian University of Applied Sciences-UPC Molecular Biology Laboratory Nutrition Research Institute

Objective: To evaluate the cytotoxic and antibacterial effect of Bixa orellana L.(B. orellana)(achiote) methanol extract against Streptococcus mutans(ATCC 25175)(S. mutans) and Streptococcus sanguinis(ATCC 10556)(S. sanguinis).Methods: Two methanol extracts of B. orellana were prepared in vitro, from the seeds and leaves. The antibacterial activity of extracts against S. mutans and S. sanguinis was evaluated using the cup-plate agar diffusion method. The minimum inhibitory concentration(MIC) was determined using the microdilution method and the cytotoxic activity was determinated by using the cell line ***: A stronger antibacterial effect was observed with the leaves methanolic extract with an inhibition zone of(19.97 ± 1.31) mm against S. mutans and(19.97 ± 1.26) mm against S. sanguinis. The methanolic extract of the seeds had an activity of(15.11 ± 1.03)mm and(16.15 ± 2.15) mm against S. mutans and S. sanguinis, respectively. The MIC of the leaf and the seed extracts against S. sanguinis was 62.5 and 125 mg/m L, respectively,and the MIC of the leaf extract against S. mutans was 62.5 mg/m L, and for the seed extract it was 31.25 mg/m L. The 50% cytotoxic concentration was 366.45 and 325.05 mg/m L for the leaves and seeds extracts, ***: The experimental findings demonstrated the antibacterial effect of the methanolic extract of B. orellana(achiote) on S. mutans and S. sanguinis. The extract of this plant is cytotoxic at high concentrations.

关键词： Antibacterial effect Medicinal plants Bixa orellana L. Cytotoxicity

Insects as alternative feed for ruminants:comparison of protein evaluation methods

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2022年第4期13卷 1143-1150页

作者： Pablo G.Toral Gonzalo Hervás Mariana Gabriela González-Rosales Alejandro G.mendoza Lizbeth E.Robles-Jiménez Pilar Frutos Instituto de Ganadería de Montaña(CSIC-University of León) Finca Marzanas s/n24346 GrullerosLeónSpain Facultad de Medicina Veterinaria y Zootecnia Universidad Autónoma del Estado de MéxicoNo.100 Instituto Literario 100Col.Centro50000 TolucaEstado de MéxicoMexico.

Background:The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich *** use of insects seems promising,but the extent of their ruminal protein degradation is largely *** parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant *** addition,although assessing ruminal N degradation represents a key first step to examine the potential of new feeds,it is a challenging task due to the lack of a reference *** study was conducted to investigate the potential of 4 insects(Tenebrio molitor,Zophobas morio,Alphitobius diaperinus and Acheta domesticus)as alternative protein sources for ruminants,using 3 methodologies:1)a regression technique based on the in vitro relationship between gas production and ammonia-N concentration;2)a conventional in vitro technique of batch cultures of ruminal microorganisms,based on filtering the incubation residue through sintered glass crucibles;and 3)the in situ nylon bag *** in vitro intestinal digestibility of the non-degraded protein in the rumen was also *** meal was used as a reference ***:Comparison of evaluation methods(regression,in vitro and in situ)did not allow to reliably select a single value of ruminal N degradation for the studied substrates,but all techniques seem to establish a similar ranking,with good correlations between methods,particularly between regression and in situ *** of the methodology,nitrogen from the 4 insects(with contents ranging from 81 to 112 g/kg of dry matter)did not show high ruminal degradation(41–76%),this value being always lower than that of soybean ***,the in vitro intestinal digestibility of non-degraded N was relatively high in all feeds(≥64%).Conclusion:Overall,these results support the potential of the 4 studied insects as alternative feedstu

关键词： In situ In vitro Livestock Nitrogen degradation Sheep

Comparison on Morphological and Optical Properties of TiO_(2) Thin Films Grown by Single-Pulse and Multi-Pulse Laser Ablation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Surface Engineered Materials and Advanced Technology 2015年第1期5卷 17-23页

作者： Yonic Penaloza-mendoza Luis Ponce-Cabrera Instituto Politecnico Nacional Cicata AltamiraAltamiraMéxico

TiO2 thin films were prepared on glass substrates using the PLD (Pulsed Laser Deposition) technique. In order to carry out the ablation process, a Nd:YAG laser was used emitting in 1064 nm wavelength at 10 Hz repetition rate, set up for operating in both single-pulse and multi-pulse regimes. A comparison of the deposition rate, the optical and morphological properties of the layers obtained from both ablation regimes was made, which showed that the multi-pulsed ablation produced layers with a higher surface quality and better optical properties.

关键词： Pulsed Laser Deposition Single-Pulse Ablation Multi-Pulse Ablation TiO_(2) Thin Film

Methylation of DAPK and THBS1 genes in esophageal gastric-type columnar metaplasia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第18期22卷 4567-4575页

作者： Roberto Herrera-Goepfert Luis F Onate-Ocana José Luis Mosqueda-Vargas Luis A Herrera Clementina Castro Julia mendoza Rodrigo González-Barrios Department of Pathology Instituto Nacional de Cancerologia (INCan) 10480 Mexico DF Mexico Clinical Research Division Instituto Nacional de Cancerologia (INCan) 10480 Mexico DF Mexico Unidad de Investigacion Biomedica en Cancer Instituto Nacional de Cancerologia (INCan)-Instituto de Investigaciones Biomedicas (IIB) Universidad Nacional Autonoma de Mexico (UNAM) 14080 Mexico DF Mexico

AIM: To explore methylation of DAPK, THBS1, CDH-1, and p14 genes, and Helicobacter pylori(H. pylori) status in individuals harboring esophageal columnar ***: Distal esophageal mucosal samples obtained by endoscopy and histologically diagnosed as gastric-type(non-specialized) columnar metaplasia, were studied thoroughly. DNA was extracted from paraffin blocks, and methylation status of deathassociated protein kinase(DAPK), thrombospondin-1(THBS1), cadherin-1(CDH1), and p14 genes, was examined using a methyl-sensitive polymerase chain reaction(MS-PCR) and sodium bisulfite modification protocol. H. pylori cag A status was determined by ***: In total, 68 subjects(33 females and 35 males), with a mean age of 52 years, were included. H. pylori cag A positive was present in the esophageal gastric-type metaplastic mucosa of 18 individuals. DAPK, THSB1, CDH1, and p14 gene promoters were methylated by MS-PCR in 40(58.8%), 33(48.5%), 46(67.6%), and 23(33.8%) cases of the 68 esophageal samples. H. pyloristatus was associated with methylation of DAPK(P = 0.003) and THBS1(P = 0.019).CONCLUSION: DNA methylation occurs in cases of gastric-type(non-specialized) columnar metaplasia of the esophagus, and this modification is associated with H. pylori cag A positive infection.

关键词： DNA methylation Esophageal columnar metaplasia Thrombospondin-1 Death-associated protein kinase Helicobacter pylori cagA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

烷基9-氮硝基喜树碱酯类的构效关系

Acta Pharmacologica Sinica 2003年第2期24卷 109-119页

作者： Panayotis PANTAZIS John mendoza Janet EARLY Anthony KOZIELSKI Nick HARRIS Beppino GIOVANELLA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA The Stehlin Foundation for Cancer Research at St. Joseph Hospital 1918 Chenevert Houston Texas 77003 USA

AIM: To study the structure-activity relationship of alkyl 9-nitrocamptothecin esters. METHODS: Two alkyl 9-nitrocamptothecin (9NC) esters 5g and 5h were prepared by esterification reactions of 9NC with valeric anhydride and heptanoic anhydride, respectively. Eight 9NC esters 5a-5h were tested for cytotoxicity against human leukemia cell lines HL-60 and U-937. Flow cytometry analysis was used to identify the cell cycle phase targeted by the esters and quantify the extent of ester-induced cell death (apoptosis). RESULTS: Esters 5b and 5c demonstrated great abilities to inhibit growth of the leukemia cells followed by induction of apoptosis; esters 5a, 5e, and 5g induced slight perturbations in the cell cycle at high concentrations; and esters 5d, 5f, and 5h were completely inactive against the cell lines tested. Thus these esters showed the cell anti-proliferative activity in an order of 5b≈5c>5a≈5e≈5g>5d≈5f≈5h. Esters 5b, 5c, and 5e were tested in vivo against various human carcinomas in nude mice grown as xenografts. Only 5b and 5c showed a significant antitumor activity. Particularly, ester 5b demonstrated an antitumor activity against a broad spectrum of human carcinomas including breast, lung, colon, pancreas, stomach, ovarian, and melanoma, etc. CONCLUSION: These esters act like prodrugs of their parental 9-nitrocamptothecin. High drug doses need to be administered to animals in order to inhibit growth, and induce regression, of human tumor xenografts in nude mice. These compounds may be developed into potent anticancer drugs due to their low toxicity.

关键词：喜树碱酯类药物前体抗肿瘤药毒性构效关系

Long-term outcome of chronic hepatitis C patients with sustained virological response to peginterferon plus ribavirin

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第4期17卷 493-498页

作者： María Trapero-Marugán Jorge mendoza María Chaparro Leticia González-Moreno José Andrés Moreno-Monteagudo María Jesús Borque Ricardo Moreno-Otero Gastroenterology-Hepatology Department University Hospital La Princesa and Princesa Research Institute Autonomous University of Madrid and CIBERehd (Centro de Investigación Biomédica en Red en Enfermedades Hepáticas y Digestivas)

AIM: To assess the clinical, biochemical and virological long-term outcome in chronic hepatitis C (CHC) patients with a sustained virological response (SVR) after peginterferon (PEG-IFN) plus ribavirin combination therapy. METHODS: One hundred and fifty three patients with a SVR after treatment with PEG-IFN plus ribavirin were included in a 5-year follow-up study in a single Spanish center, based on standard clinical practice. Clinical anamnesis, biochemical analysis, hepatitis C virus RNA and alpha-fetoprotein measurement, ultrasonography and transient elastography were performed annually. RESULTS: The mean follow-up period of the 153 patients was 76 ± 13 mo after they obtained a SVR. Five patients (3.26%) presented with cirrhosis before treatment and 116 (75.8%) had genotype 1. No patient showed evidence of hepatic decompensation. One patient (0.65%) developed a hepatocellular carcinoma at month 30 after achieving SVR. There were no virological relapses during this follow-up period. Persistently elevated alanine aminotransferase was found in only one patient (0.65%). At the end of the 5-year follow-up, the mean value of transient elastography was 7 ± 4.3 kPa (F1). There were no deaths and no other tumors. CONCLUSION: The long-term outcome of 153 CHC patients with SVR to PEG-IFN plus ribavirin was good. No evidence of a virological relapse was seen. One patient (0.65%) developed a hepatocellular carcinoma.

关键词： Chronic hepatitis C Peginterferon Ribavirin Sustained virological response Long-term effects

Repeat recurrence of takotsubo cardiomyopathy related to inhaled beta-2-adrenoceptor agonists

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Cardiology 2012年第6期4卷 211-213页

作者： Ivan mendoza Gian M Novaro Department of Cardiology Cleveland Clinic Florida Weston FL 33331 United States

Takotsubo cardiomyopathy (also referred to as transient apical ballooning syndrome, broken heart syndrome or stress cardiomyopathy) is an increasingly recognized entity in the western world typically characterized by reversible left ventricular dysfunction that develops in the setting of acute severe emotional or physical stress. Increased catecholamine levels have been proposed to play a central role in the pathogenesis of the disease, although the specific pathophysiology of this condition remains elusive at the present moment. In recent times, there have been reports of takotsubo cardiomyopathy (TC) following medical interventions such as invasive or surgical procedures or specific medical regimens. In the current report, we present a patient with multiple recurrences of TC triggered by the same medical therapeutic intervention; in our particular case, repetitive exposure to inhaled beta-2-adrenoceptor agonist.

关键词： Takotsubo Beta-2-adrenoreceptor agonist Recurrence Ventricular dysfunction Heart failure

Personalizing Insomnia:Adverse Health Outcomes from Adulthood to Childhood

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Personalizing Insomnia:Adverse Health Outcomes from Adulthoo...

Behavioral Sleep Medicine:the Insomnia success and beyond

第二届中国睡眠研究会青年学术论坛暨四川省医学会第四次睡眠医学学术会议

作者： Julio Fernandez-mendoza Assistant Professor Sleep Research & Treatment Center Penn State Milton Hershey Medical Center Penn State Hershey College of Medicine Hershey PA

＜正＞～～

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Behavioral Sleep Medicine:the Insomnia success and beyond