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A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera lucia Pereira-Chioccola Thelma Suely Okay lucia maria almeida braz Laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil Laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil Laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil Laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil Laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

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Update on the use of 45S5 bioactive glass in the treatment of bone defects in regenerative medicine

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World Journal of Orthopedics 2024年第3期15卷 204-214页

作者： Dayane maria braz Nogueira Marcelie Priscila de Oliveira Rosso Daniela Vieira Buchaim mariana Schutzer Ragghianti Zangrando Rogério Leone Buchaim Department of Prosthodontics and Periodontics Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil Department of Biological Sciences Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil Medical School University Center of AdamantinaAdamantina 17800-000Brazil Postgraduate Program in Structural and Functional Interactions in Rehabilitation Postgraduate DepartmentUniversity of MaríliaMarília 17525-902Brazil

Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive *** research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is *** achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last *** search used the keywords“bioglass 45S5 AND bone defect”in *** found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed *** of these studies compared 45S5 BG with other cement or scaffold *** comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic ***,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.

关键词： Biocompatible materials Bioglass Bone regeneration

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Multi-omic study to unmask genes involved in prostate cancer development in amulti-case family

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Cancer Communications 2024年第3期44卷 443-447页

作者： lucia Chica-Redecillas Sergio Cuenca-Lopez Eduardo Andres-Leon Laura Carmen Terron-Camero Blanca Cano-Gutierrez Jose Manuel Cozar Jose Antonio Lorente Fernando Vazquez-Alonso Luis Javier Martinez-Gonzalez maria Jesus Alvarez-Cubero GENYO Centre for Genomics and Oncological ResearchPfizerUniversity of GranadaAndalusian Regional GovernmentPTS GranadaGranadaGranadaSpain Department of Biochemistry Molecular Biology III and ImmunologyFaculty of MedicineUniversity of GranadaPTS GranadaGranadaGranadaSpain Bioinformatics Unit Institute of Parasitology and Biomedicine“López-Neyra”Spanish National Research CouncilGranadaGranadaSpain Nursery Service.University Hospital Clínico San Cecilio GranadaGranadaSpain Urology Department University Hospital Virgen de las NievesGranadaGranadaSpain Legal Medicine and Toxicology Department Faculty of MedicineUniversity of GranadaPTS GranadaGranadaGranadaSpain Ibs Biosanitary Research InstituteGranadaGranadaSpain

Dear Editor,Hereditary prostate cancer(PC)comprises 5%-10%of all PC *** increased risk of PC in menwith a family his-tory of the disease is well known and is commonly caused by germline mutations,leading to clinical guidelines men-tioning various genes for identifying high-risk ***,the complex inheritance patterns involvingmul-tiple single nucleotide polymorphisms(SNPs)make it a genetically heterogeneous disease,with genetic testing still in its early stages.

关键词： cancer clinical cases

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Lower Continental Crusts Involved in the Alpine Orogeny:New Insight from the Santa lucia Nappe Metasediments(Corsica,France)

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Acta Geologica Sinica(English Edition) 2024年第4期98卷 904-920页

作者： Chiara FRASSI maria DI ROSA Luca PANDOLFI Michele MARRONI Dipartimento di Scienze della Terra Universitàdi PisaVia Santa Maria 5356126 PisaItaly Istituto di Geoscienze e Georisorse IGG-CNRVia Moruzzi50124 PisaItaly

In the Alpine Corsica(France),the Santa lucia Nappe represents a peculiar unit preserving the unique relicts of Paleozoic lower to medium continental *** consists of composite basement affected by Permian granulite facies metamorphic conditions unconformably covered by a Late Cretaceous clastic sequence(Tomboni Conglomerate and Tralonca Flysch)affected by polyphase deformation and *** this work,we present a new reconstruction of the deformation events registered by the Tralonca Flysch during the Alpine *** D1 phase was testified by rare isoclinal *** D2 phase produced a continuous foliation and a map-scale sheath-fold with a top-to-W sense of *** D3 phase produced E-verging non-cylindrical folds and S3 crenulation cleavage that is not associated to metamorphic *** present the first temperature-pressure-deformation(P-T-d)path for the Tralonca Flysch,demonstrating that the Santa lucia Nappe underwent accretion and then first stage exhumation in the Alpine wedge during the D1 phase with pressure and temperature peaks both occurred under blueschist metamorphic *** D2 phase occurred at lower pressure-temperature conditions during a second stage *** pressure-temperaturedeformation path is comparable with those of the Lower Units(i.e.,the subducted continental units of Alpine Corsica)suggesting a common geodynamic history.

关键词： blueschist-facies metamorphism P-T-d path Santa lucia Nappe Corsica Alpine orogeny

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Hyperkalemia in Patients on Peritoneal Dialysis: Clinical Use Experience with New Potassium-Binders

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Open Journal of Nephrology 2024年第3期14卷 324-333页

作者： Gennaro Argentino Mario Iorio Alessandra Antonia Mele Andrea Camocardi Enrica Emanuela Cascone maria Elena Liberti Adelia Sagliocca Andrea Pota Luigi Russo maria Luisa Sirico Germano Terzini Michele Cavasso Chiara Mennillo Francesca Nettuno Raffaele Genualdo Giuseppe Surfaro lucia Di Micco UOC Nefrologia e Dialisi Ospedale del Mare Naples Italy UOC Chirurgia Generale Ospedale del Mare Naples Italy

Background: Patients with end-stage kidney disease (ESKD) on maintenance dialysis have a high risk of developing hyperkalemia. In addition to traditional approaches, a new option for the management of patients on dialysis includes the use of a potassium binder, sodium zirconium cyclosilicate (SZC). We evaluated the effect and safety of SZC in patients with chronic PD. Objective: To present a case series that illustrates the real-world use of new potassium-binders in hyperkalemic patients on peritoneal dialysis. Methods: This case series collected 9 patients on PD with baseline potassium values > 5.5 mmol/l and who were treated with SZC 5 g once a day. Data were collected at baseline and at 1, 2, and 3 months after initiation of treatment. Results: The median age of patients was 64.5 years and the median duration of observation was 90 ± 5 days. It was observed that median serum potassium decreased (5.8 mmol/l at baseline with a range of 5.8 mmol/L - 6.8 mmol/L versus 4.5 in the third month with a range of 3.6 mmol/L - 5.3 mmol/L) after SZC treatment. Adverse events were observed in 2 (22.2%). The unique adverse event was constipation and presented in 2 patients (22.2%). Constipation was mild and transient during the observation period. No adverse events of special interest were reported. Conclusion: Normokalemia was established and maintained in this series of patients treated with SZC. No episodes of hyper- or hypo-kalemia were observed. SCZ had a good safety profile and was well tolerated over 3 months.

关键词： SZC Sodium Zirconium Cyclosilicate Hyperkalaemia Peritoneal Dialysis

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Acromyrmex ameliae sp. n. （Hymenoptera： Formicidae）： A new social parasite of leaf-cutting ants in brazil

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Insect Science 2007年第3期14卷 251-257页

作者： DANIVAL JOSE DE SOUZA ILKA maria FERNANDES SOARES TEREZINHA maria CASTRO DELLA lucia Institut de Recherche sur la Biologie de 1'Insecte Université Francois Rabelais Tours France Departamento de Biologia Animal Laboratório de Ecologia de Comunidades Departamento de Biologia Geral Universidade Federal de Vicosa MG 36570-000 Brazil

The fungus-growing ants （Tribe Attini） are a New World group of〉 200 species, all obligate symbionts with a fungus they use for food. Four attine taxa are known to be social parasites of other attines. Acromyrmex （ Pseudoatta） argentina argentina and Acromyrmex （Pseudoatta） argentina platensis （parasites of Acromyrmex lundi）, and Acromyrmex sp. （a parasite of Acromyrmex rugosus） produce no worker caste. In contrast, the recently discovered Acromyrmex insinuator （a parasite of Acromyrmex echinatior） does produce workers. Here, we describe a new species, Acromyrmex ameliae, a social parasite of Acromyrmex subterraneus subterraneus and Acromyrmex subterraneus brunneus in Minas Gerais, Brasil. Like A. insinuator, it produces workers and appears to be closely related to its hosts. Similar social parasites may be fairly common in the fungus-growing ants, but overlooked due to the close resemblance between parasite and host workers.

关键词： Acromyrmex leaf-cutting ants social evolution social parasitism

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Reproductive ecology of the seabob shrimp Xiphopenaeus kroyeri(Heller,1862)in a coastal area of Southern brazil

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中国海洋湖沼学报：英文版 2016年第1期34卷 125-135页

作者： GRABOWSKI Raphael Cezar NEGREIROS-FRANSOZO maria lucia CASTILHO Antonio Leao Zoology Department Botucatu Biosciences InstituteSao Paulo State UniversityBotucatu

The predictability of certain environmental factors that affect the life cycle of the seabob shrimp Xiphopenaeus kroyeri(Heller,1862) was evaluated in a study of its reproductive biology in an area adjacent to Babitonga Bay,State of Santa Catarina,brazil. Monthly sampling was conducted from July 2010 through June 2011 at depths of 5,8,11,14,and 17 m.76 004 individuals were obtained,with a pronounced peak in absolute abundance in austral autumn(34 208),coinciding with the annual closed season from March to May. Grain size composition of the sediment showed the closest relationship to the distribution of individuals(multiple linear regression,P <0.05),related to their burying habit. The observed correlations between the abundance of reproductive males(bearing spermatophores) and females with spent gonads(cross-correlation,P <0.05),and between reproductive males and reproductive females(with a 1-month lag) suggest that the peak of reproductive males preceded the peak offemale ones. This result agrees with the pattern expected for females,which copulate in post-ecdysis(spent gonads). Spawning seemed to take place at greater depths,as evidenced by the concentration of reproductive females in these areas. The reproductive activities observed here confirm that this species follows a tropical/subtropical reproductive pattern,spawning continuously throughout the year,with the highest peaks in spring and autumn. The data indicate that the juvenile recruitment period observed in August-September resulted from the reproductive output noted in April–May. Additionally,the reproductive period recorded in November led to the juvenile peak observed in March–May.

关键词： crustacean fisheries Dendrobranchiata environmental effects recruitment spawning

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Early detection of tomato bacterial canker by reflectance indices

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Information Processing in Agriculture 2022年第2期9卷 184-194页

作者： Gabriela Cordon Carolina Andrade lucia Barbara Ana maria Romero Universidad de Buenos Aires Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicasInstituto de Investigaciones Fisiolo´gicas y Ecolo´gicas Vinculadas a la Agricultura(IFEVA)Facultad de Agronomı´aBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aA´rea de Educacio´n AgropecuariaBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aCa´tedra de Fitopatologı´aBuenos AiresArgentina

Early detection of infected asymptomatic plants could prevent the dissemination of tomatobacterial canker throughout the crop and, thus, reduce associated economic losses. Reflectance spectroscopy has been proposed for the detection of diseases in plants, mainlycaused by foliar fungal pathogens. In contrast, bacterial canker is a vascular disease thatdoes not produce visible foliar symptoms until it is too late to avoid contagion to otherplants. The effects of the disease on the conductivity of the xylem have an impact onthe water content of the leaves;initially, a subtle effect that cannot be assessed with thenaked eye but can be evaluated by reflectance spectroscopy. The objective of this workwas to identify indices, based on the reflectance spectral signature of the plants, for thedetection of tomato plants affected by bacterial canker prior to the appearance of symptoms. To verify that the proposed methodology is applicable to a broad range of tomatogenotypes, two experiments were carried out with different tomato cultivars grown in potsand another on the ground in a greenhouse. Plants were inoculated with the pathogenicbacteria and the reflectance spectrum of the apical leaflet of two leaves per plant wereobtained before symptoms expression;several spectral indices were calculated from thereflectance spectra. Three indices, of the shortwave-infrared zone, allowed the detectionof bacterial canker inoculated plants in a fast and non-destructive way, up to one weekbefore symptoms were visible: Normalized Difference Water Index (NDWI), Simple Ratioof Water Index (SRWI) and Water Index 1 180 (WI1180). Our research demonstrates, forthe first time, the usefulness of spectral indices sensitive to water content for the identifi-cation of diseased plants. These indices arise as a promising tool for the early detection ofvascular plant diseases, that could be integrated into sustainable management plans.

关键词： Clavibacter michiganensis subsp. michiganensis Bacterial wilt Reflectance indices Solanum lycopersicum L

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Non-operative successful management of a perforated small bowel diverticulum

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World Journal of Gastroenterology 2014年第48期20卷 18477-18479页

作者： Melissa M Levack maria lucia Madariaga Haytham MA Kaafarani Division of Trauma Department of Surgery SurgicalCritical Care Massachusetts General Hospital Harvard MedicalSchool Boston MA 02114 United States

Jejunoileal diverticula are rare and generally asymptomatic. In the few cases of patients who develop complications such as diverticulitis, perforation, obstruction, and/or hemorrhage, conventional treatment consists of surgical resection. We describe a case of perforated jejunoileal diverticulum with localized abscess and highlight the merits of surgical vs medical management. The patient is a 77-year-old male who presented with sharp, constant abdominal pain just inferior to the umbilicus. Administration of intravenous antibiotics results in complete and long-term resolution of the patient’s symptoms. In this report, we establish a framework for safely treating perforated small bowel diverticulum without surgical exploration.

关键词： Jejunal diverticula Diverticulitis Small bowel perforation Antibiotics Abscess

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Why and how to use the SeqCode

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mLife 2024年第1期3卷 1-13页

作者： Wiliam B.Whitman maria Chuvochina Brian P.Hedlund Konstantinos T.Konstantinidis Marike Palmer Luis M.Rodriguez-R lain Sutcliffe Fengping Wang Deparment of Microbioogy University of GeorgiaAthensGeorgiaUAA Schoolof Chemistry and Molecular Biosciences Australian CentreforEogenomicsThe Universty of QueenslandSt LuciaAustralia University of Nevada Las Vegas Las VegasNevadaUSA Schol of Civil and Environmental Engineering and School of Biological SciencesGeorgia Institute of TechnologyAtlantaGeorgiaUSA Department of Microbiology University of ManitobaWinnipegManitobaCanada School of Life Sciences University of Nevada Las VegasLas VegasNevadaUSA Department of Microbiology and Digital Science Center(DiSC) University of InnsbruckInnsbruckAustria Faculty of Health&Life Sciences Northumbria UniversityNewcastle upon TyneUK School of Oceanography International Center for Deep Life InvestigationShanghai Jiao Tong UniversityShanghaiChina

The SeqCode,formally called the Code of Nomenclature of Prokaryotes Described from Sequence Data,is a new code of nomenclature in which genome sequences are the nomenclatural types for the names of prokaryotic *** similar to the International Code of Nomenclature of Prokaryotes(ICNP)in structure and rules of priority,it does not require the deposition of type strains in international culture ***,it allows for the formation of permanent names for uncultured prokaryotes whose nearly complete genome sequences have been obtained directly from environmental DNA as well as other prokaryotes that cannot be deposited in culture *** the diversity of uncultured prokaryotes greatly exceeds that of readily culturable prokaryotes,the SeqCode is the only code suitable for naming the majority of prokaryotic *** start date of the SeqCode was January 1,2022,and the online Registry(https://***)was created to ensure valid publication of *** SeqCode recognizes all names validly published under the ICNP before *** that date,names validly published under the SeqCode compete with ICNP names for *** a result,species can have only one name,either from the SeqCode or ICNP,enabling effective communication and the creation of unified taxonomies of uncultured and cultured *** SeqCode is administered by the SeqCode Committee,which is comprised of the SeqCode Community and elected administrative *** with an interest in the systematics of prokaryotes is encouraged to join the SeqCode Community and participate in the development of this resource.

关键词： genome sequences metagenome-assembled genomes nomenclature SeqCode

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