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A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera lucia Pereira-Chioccola Thelma Suely Okay lucia maria almeida braz Laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil Laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil Laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil Laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil Laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

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Update on the use of 45S5 bioactive glass in the treatment of bone defects in regenerative medicine

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World Journal of Orthopedics 2024年第3期15卷 204-214页

作者： Dayane maria braz Nogueira Marcelie Priscila de Oliveira Rosso Daniela Vieira Buchaim mariana Schutzer Ragghianti Zangrando Rogério Leone Buchaim Department of Prosthodontics and Periodontics Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil Department of Biological Sciences Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil Medical School University Center of AdamantinaAdamantina 17800-000Brazil Postgraduate Program in Structural and Functional Interactions in Rehabilitation Postgraduate DepartmentUniversity of MaríliaMarília 17525-902Brazil

Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive *** research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is *** achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last *** search used the keywords“bioglass 45S5 AND bone defect”in *** found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed *** of these studies compared 45S5 BG with other cement or scaffold *** comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic ***,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.

关键词： Biocompatible materials Bioglass Bone regeneration

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Multi-omic study to unmask genes involved in prostate cancer development in amulti-case family

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Cancer Communications 2024年第3期44卷 443-447页

作者： lucia Chica-Redecillas Sergio Cuenca-Lopez Eduardo Andres-Leon Laura Carmen Terron-Camero Blanca Cano-Gutierrez Jose Manuel Cozar Jose Antonio Lorente Fernando Vazquez-Alonso Luis Javier Martinez-Gonzalez maria Jesus Alvarez-Cubero GENYO Centre for Genomics and Oncological ResearchPfizerUniversity of GranadaAndalusian Regional GovernmentPTS GranadaGranadaGranadaSpain Department of Biochemistry Molecular Biology III and ImmunologyFaculty of MedicineUniversity of GranadaPTS GranadaGranadaGranadaSpain Bioinformatics Unit Institute of Parasitology and Biomedicine“López-Neyra”Spanish National Research CouncilGranadaGranadaSpain Nursery Service.University Hospital Clínico San Cecilio GranadaGranadaSpain Urology Department University Hospital Virgen de las NievesGranadaGranadaSpain Legal Medicine and Toxicology Department Faculty of MedicineUniversity of GranadaPTS GranadaGranadaGranadaSpain Ibs Biosanitary Research InstituteGranadaGranadaSpain

Dear Editor,Hereditary prostate cancer(PC)comprises 5%-10%of all PC *** increased risk of PC in menwith a family his-tory of the disease is well known and is commonly caused by germline mutations,leading to clinical guidelines men-tioning various genes for identifying high-risk ***,the complex inheritance patterns involvingmul-tiple single nucleotide polymorphisms(SNPs)make it a genetically heterogeneous disease,with genetic testing still in its early stages.

关键词： cancer clinical cases

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Lower Continental Crusts Involved in the Alpine Orogeny:New Insight from the Santa lucia Nappe Metasediments(Corsica,France)

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Acta Geologica Sinica(English Edition) 2024年第4期98卷 904-920页

作者： Chiara FRASSI maria DI ROSA Luca PANDOLFI Michele MARRONI Dipartimento di Scienze della Terra Universitàdi PisaVia Santa Maria 5356126 PisaItaly Istituto di Geoscienze e Georisorse IGG-CNRVia Moruzzi50124 PisaItaly

In the Alpine Corsica(France),the Santa lucia Nappe represents a peculiar unit preserving the unique relicts of Paleozoic lower to medium continental *** consists of composite basement affected by Permian granulite facies metamorphic conditions unconformably covered by a Late Cretaceous clastic sequence(Tomboni Conglomerate and Tralonca Flysch)affected by polyphase deformation and *** this work,we present a new reconstruction of the deformation events registered by the Tralonca Flysch during the Alpine *** D1 phase was testified by rare isoclinal *** D2 phase produced a continuous foliation and a map-scale sheath-fold with a top-to-W sense of *** D3 phase produced E-verging non-cylindrical folds and S3 crenulation cleavage that is not associated to metamorphic *** present the first temperature-pressure-deformation(P-T-d)path for the Tralonca Flysch,demonstrating that the Santa lucia Nappe underwent accretion and then first stage exhumation in the Alpine wedge during the D1 phase with pressure and temperature peaks both occurred under blueschist metamorphic *** D2 phase occurred at lower pressure-temperature conditions during a second stage *** pressure-temperaturedeformation path is comparable with those of the Lower Units(i.e.,the subducted continental units of Alpine Corsica)suggesting a common geodynamic history.

关键词： blueschist-facies metamorphism P-T-d path Santa lucia Nappe Corsica Alpine orogeny

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Early detection of tomato bacterial canker by reflectance indices

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Information Processing in Agriculture 2022年第2期9卷 184-194页

作者： Gabriela Cordon Carolina Andrade lucia Barbara Ana maria Romero Universidad de Buenos Aires Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicasInstituto de Investigaciones Fisiolo´gicas y Ecolo´gicas Vinculadas a la Agricultura(IFEVA)Facultad de Agronomı´aBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aA´rea de Educacio´n AgropecuariaBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aCa´tedra de Fitopatologı´aBuenos AiresArgentina

Early detection of infected asymptomatic plants could prevent the dissemination of tomatobacterial canker throughout the crop and, thus, reduce associated economic losses. Reflectance spectroscopy has been proposed for the detection of diseases in plants, mainlycaused by foliar fungal pathogens. In contrast, bacterial canker is a vascular disease thatdoes not produce visible foliar symptoms until it is too late to avoid contagion to otherplants. The effects of the disease on the conductivity of the xylem have an impact onthe water content of the leaves;initially, a subtle effect that cannot be assessed with thenaked eye but can be evaluated by reflectance spectroscopy. The objective of this workwas to identify indices, based on the reflectance spectral signature of the plants, for thedetection of tomato plants affected by bacterial canker prior to the appearance of symptoms. To verify that the proposed methodology is applicable to a broad range of tomatogenotypes, two experiments were carried out with different tomato cultivars grown in potsand another on the ground in a greenhouse. Plants were inoculated with the pathogenicbacteria and the reflectance spectrum of the apical leaflet of two leaves per plant wereobtained before symptoms expression;several spectral indices were calculated from thereflectance spectra. Three indices, of the shortwave-infrared zone, allowed the detectionof bacterial canker inoculated plants in a fast and non-destructive way, up to one weekbefore symptoms were visible: Normalized Difference Water Index (NDWI), Simple Ratioof Water Index (SRWI) and Water Index 1 180 (WI1180). Our research demonstrates, forthe first time, the usefulness of spectral indices sensitive to water content for the identifi-cation of diseased plants. These indices arise as a promising tool for the early detection ofvascular plant diseases, that could be integrated into sustainable management plans.

关键词： Clavibacter michiganensis subsp. michiganensis Bacterial wilt Reflectance indices Solanum lycopersicum L

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Exercise training mode effects on myokine expression in healthy adults:A systematic review with meta-analysis

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Journal of Sport and Health Science 2024年第6期13卷 764-779页

作者： Francesco Bettariga Dennis R.Taaffe Daniel A.Galvao Pedro Lopez Chris Bishop Anna maria Markarian Valentina Natalucci Jin-Soo Kim Robert U.Newton Exercise Medicine Research Institute Edith Cowan UniversityJoondalupWA 6027Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia Pleural Medicine Unit Institute for Respiratory HealthPerthWA 6009Australia Medical School Faculty of Health&Medical SciencesUniversity of Western AustraliaPerthWA 6009Australia Grupo de Pesquisa em Exercıcio para Populacoes Clınicas(GPCLIN) Universidade de Caxias do SulCaxias do SulRio Grande do Sul 95070-560Brazil London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK Department of Pathophysiology and Transplantation University of MilanMilan 20133Italy School of Human Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4072Australia

Background:The benefits of exercise are well known;however,many of the underlying molecular mechanisms are not fully *** muscle secretes myokines,which mediate muscleorgan *** regulate satellite-cell proliferation and migration,inflammatory cascade,insulin secretion,angiogenesis,fatty oxidation,and cancer *** date,the effects of different exercise modes(namely,aerobic and resistance exercise)on myokine response remain to be *** is crucial considering the clinical implementation of exercise to enhance general health and wellbeing and as a medical ***:A systematic search was undertaken in PubMed,MEDLINE,CINAHL,Embase,SPORTDiscus,andWeb of Science in April *** studies examining the effects of a single bout of exercise on interleukin15(IL-15),irisin,secreted protein acidic and rich in cysteine(SPARC),oncostatinM(OSM),and decorin were included.A random-effects meta-analysis was also undertaken to quantify the magnitude of ***:Sixty-two studies were included(n=1193).Overall,exercise appeared to induce small to large increases in myokine expression,with effects observed immediately after to 60 min post-exercise,although these were mostly not statistically *** aerobic and resistance exercise resulted in changes in myokine levels,without any significant difference between training modes,and with the magnitude of change differing across *** levels returned to baseline levels within 180 min to 24 h ***,owing to potential sources of heterogeneity,most changes were not statistically significant,indicating that precise conclusions cannot be ***:Knowledge is limited but expanding with respect to the impact of overall and specific effects of exercise on myokine expression at different time points in the systemic *** research is required to investigate the effects of different exercise modes at multiple time points on myok

关键词： Myokine Resistance exercise Aerobic exercise Cytokine Systemic circulation

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Why and how to use the SeqCode

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mLife 2024年第1期3卷 1-13页

作者： Wiliam B.Whitman maria Chuvochina Brian P.Hedlund Konstantinos T.Konstantinidis Marike Palmer Luis M.Rodriguez-R lain Sutcliffe Fengping Wang Deparment of Microbioogy University of GeorgiaAthensGeorgiaUAA Schoolof Chemistry and Molecular Biosciences Australian CentreforEogenomicsThe Universty of QueenslandSt LuciaAustralia University of Nevada Las Vegas Las VegasNevadaUSA Schol of Civil and Environmental Engineering and School of Biological SciencesGeorgia Institute of TechnologyAtlantaGeorgiaUSA Department of Microbiology University of ManitobaWinnipegManitobaCanada School of Life Sciences University of Nevada Las VegasLas VegasNevadaUSA Department of Microbiology and Digital Science Center(DiSC) University of InnsbruckInnsbruckAustria Faculty of Health&Life Sciences Northumbria UniversityNewcastle upon TyneUK School of Oceanography International Center for Deep Life InvestigationShanghai Jiao Tong UniversityShanghaiChina

The SeqCode,formally called the Code of Nomenclature of Prokaryotes Described from Sequence Data,is a new code of nomenclature in which genome sequences are the nomenclatural types for the names of prokaryotic *** similar to the International Code of Nomenclature of Prokaryotes(ICNP)in structure and rules of priority,it does not require the deposition of type strains in international culture ***,it allows for the formation of permanent names for uncultured prokaryotes whose nearly complete genome sequences have been obtained directly from environmental DNA as well as other prokaryotes that cannot be deposited in culture *** the diversity of uncultured prokaryotes greatly exceeds that of readily culturable prokaryotes,the SeqCode is the only code suitable for naming the majority of prokaryotic *** start date of the SeqCode was January 1,2022,and the online Registry(https://***)was created to ensure valid publication of *** SeqCode recognizes all names validly published under the ICNP before *** that date,names validly published under the SeqCode compete with ICNP names for *** a result,species can have only one name,either from the SeqCode or ICNP,enabling effective communication and the creation of unified taxonomies of uncultured and cultured *** SeqCode is administered by the SeqCode Committee,which is comprised of the SeqCode Community and elected administrative *** with an interest in the systematics of prokaryotes is encouraged to join the SeqCode Community and participate in the development of this resource.

关键词： genome sequences metagenome-assembled genomes nomenclature SeqCode

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Occurrence and patterns of nutritional traits and polycyclic aromatic hydrocarbons(PAHs)in sea cucumber(Holothuria poli)tissues:benefits and risk for human health

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Food Quality and Safety 2022年第2期6卷 287-309页

作者： Francesca Biandolino Isabella Parlapiano lucia Spada Antonella Di Leo maria Calo Giovanni Fanelli Ermelinda Prato Santina Giandomenico CNR-IRSA National Research CouncilWater Research Institute--Via Roma 3TarantoItaly

Objectives:The paper evaluates the benefit and risk for human health associated with consumption of sea cucumber Holothuria poli(*** from Italian coasts(Central Mediterranean Sea).Materials and Methods:Body wall(BW),internal tunic(ITu),muscle bands(MBs),alimentary canal(AC),gonad(Gd),and respiratory tree(RT)of *** were analyzed for proximate ***,amino acids(AAs),fatty acids(FAs)and polycyclic aromatic hydrocar bons(PAHs)were determined with high-performance liquid chromatography coupled with ultraviolet-visible spectroscopy(UPLC UV/Vis),gas chromatography-flame ionization detector(GC-FID)and gas chromatography mass spectrometry(GC-MS),***:Differences in the contents of total amino acids(TAAs)occurred based on tissue and sex,with AC and MB of female and Gd of male showing higher contents(range 47.8-60.2 g/kg we weight(ww)).Glycine and glutamic acid were the most *** fatty acid(PUFA)was the major class of FAs and arachidonic acid and eicosapentenoic acid(EPA)were the predominant PUFA.n-3 PUFA showed higher content in Gd,AC,and RT,indicating higher quality.A favorable n-3/n-6 in the range of 1.04-1.67 was *** showed values ranging from 23 to 207μg/kg ww with the highest levels in Gd and AC tissues and the lower in ***[alpyrene,the most toxic compound,was detected in all tissues,of both sexes,at levels of 1.5-18μg/kg ***:All tissues of ***,although with differences among them,are valuable food and can contribute for a healthy *** cancer risk(CR)values for Gd and AC tissues were above the considerable CR threshold of one in 10000 established by the United States Environmental Protection Agency(USEPA)for high ingestion rate of this seafood.

关键词： Holothuria poli,nutitional quality polycyclic aromatic hydrocarbons risk assessment estimated weekly intake cancer risk

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Emerging environmental stressors and oxidative pathways in marine organisms:Current knowledge on regulation mechanisms and functional effects

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BIOCELL 2022年第1期46卷 37-49页

作者： MAURA BENEDETTI maria ELISA GIULIANI MARICA MEZZELANI ALESSANDRO NARDI lucia PITTURA STEFANIA GORBI FRANCESCO REGOLI Dipartimento di Scienze della Vitaedell’Ambiente UniversitàPolitecnica delle MarcheAncona60131Italy

Oxidative stress is a critial condition derived from the imbalance between the genention of reactive oxygen species and the sophisticated network of antioidant *** pollut ants and envronmental factors can afet this system through connected mechanisms,indirect relationships,and cascade effects from pre-transcriptional to catalytic level,by either enhancing intracellular ROS formation or impairing antioxidant *** review summariaces the current kmnowledge on the pro oxidant challenges from emerging environmental stressors threatening marine organisms,such as pharmaceutials microplastics and dimate related ocean *** will be placed on oxidative pathways,including signaling proteins and transcription factors involved in regulation of antioxidant *** insights and lack of knowdedge will be pointed out by presenting single and combined efects of multiple stessors,unraveling questions to be adresed by future resarch in marine ectoicology.

关键词： Antioxidant Pharmaceuticals Microplastics/Nanoplastics Ocean acidification Thermal stress

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Ontogenetic niche specialization of the spider crab Libinia ferreirae associated with the medusa Lychnorhiza lucerna

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Current Zoology 2022年第5期68卷 549-559页

作者： Geslaine Rafaela Lemos Gonçalves Milena Regina Wolf mariana Antunes Felipe Wanderley Amorim maria lucia Negreiros-Fransozo Antonio Leão Castilho Group of Studies on Crustacean Biology Ecology and Culture-NEBECCBiosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil and Laboratory of Pollination Ecology and Interactions-LEPI Biosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil

Symbiotic relationships in marine environments are not fixed and can change throughout the animal’s *** study investigated the ontogeny of symbiosis of the spider crab Libinia ferreirae with the host medusa Lychnorhiza *** described the type of relationship,the temporal correlation among species,and food *** than 50%of the sampled crabs were symbionts,most in early life *** highest number of crabs found in a single medusa was *** was observed throughout most of the year but was more evident in warm *** crab has many benefits in this relationship with a *** is the use of food resources captured by the medusa,primarily *** the crab steals the medusa’s food,it is a kleptoparasitic *** is a niche partition between symbiont and the free-living crabs as they occupy different habitats and use nonoverlapping food *** research reported that symbiosis first developed during the crab’s last larval phase(megalopa)when crab and medusa are in the same *** of the crab’s behavior shows that symbiosis occurs when the crab can grab to the medusa when the host touches the sea *** crab also took advantage of water currents,releasing itself from the substrate and then drifting toward the *** symbiotic relationship that crabs have with the medusa provides then with a nursery,food resources,shelter,dispersion,and decreased competition with free-living adult crabs,all essential for the crab’s survival.

关键词： behavior development kleptoparasitism megalopa niche segregation

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