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Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

World Journal of Gastroenterology 2005年第29期11卷 4497-4504页

作者： Claudia lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel Center of Bone Marrow Transplantation University Hospital Hamburg-Eppendorf Martinistrasse 52 D-20246 Hamburg Germany Department of Pediatric Surgery University Hospital Hamburg-Eppendorf Martinistrasse 52 D-20246 Hamburg Germany

AIM： The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stern cell cultures. We investigated the potential of rat mesenchymal stem cells （MSC） from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore, we assessed the influence of cocultured liver cells on induction of liver-specific gene expression. METHODS： Mesenchymal stem cells were marked with green fluorescent protein （GFP） by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF, EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP＋ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thyl and the hepatocytic markers CK-18, albumin, CK-19, and AFP was performed in the different cell populations. RESULTS： Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18, CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression, CONCLUSION： The results indicate that （1） rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and （2） that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC.

关键词： Mesenchymal stem cells Liver-specific differentiation Coculture

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Support for obesity policy: The effect of perceptions of causes for obesity and national identity in Canada

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Open Journal of Preventive Medicine 2012年第4期2卷 478-489页

作者： Ryan lange Guy Faulkner Faculty of Kinesiology and Physical Education University of Toronto Toronto Canada

Interventions in Canada to address obesity have largely been limited to individual-level change through education rather than a population-level public policy approach. Little is known about obesity policy support in Canada, and substantial national variation in obesity policy support prevents direct transferability of these policies among nations. Our study recruited 521 young adults for an online survey through leaflets and flyers. We investigated how respondents’ demographics, health characteristics, political attitudes, beliefs regarding causes of obesity, and national identity affected their support for obesity policy. Results showed that there is high support for many obesity policies among Canadian young adults. Including Canadian national identity in regression models was significant in explaining obesity policy support beyond the combined effect of other predictors. Further exploration of national identity, in Canada and elsewhere, has implications for understanding obesity policy support that might assist policy makers in making more informed decisions in addressing obesity.

关键词： Obesity Policy National Identity Canada Opinion Government

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Biophotonic sensors with integrated Si_(3)N_(4)-organic hybrid (SiNOH) lasers for point-of-care diagnostics

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Light(Science & Applications) 2021年第4期10卷 648-659页

作者： Daria Kohler Gregor Schindler Lothar Hahn Johannes Milvich Andreas Hofmann Kerstin lange Wolfgang Freude Christian Koos Institute of Photonics and Quantum Elearo nics(IPQ) Karlsruhe Institute of Technology(KIT)Engesserstrasse 576131 KarlsruheGermany lnstitute of Microstructure Technology(IMT) Karlsruhe Institute of Technology(KIT)Hermann-von-Helmholtz-Platz 176344 Eggenstein-LeopoldshafenGermany Robert Bosch GmbH Robert-Bosch-Campus 171272 RenningenGermany lnstitute for Autom ation and Applied Informatics(IAI) Karlsruhe Institute of Technology(KIT)Hermann-von-Helmholtz-Platz 176344 Eggenstein-LeopoldshafenGermany

Early and efficient disease diagnosis with low-cost point-of-care devices is gaining importance for personalized medicine and public health *** this context,waveguide-(WG)-based optical biosensors on the siliconnitride(Si_(3)N_(4))platform represent a particularly promising option,offering highly sensitive detection of indicative biomarkers in multiplexed sensor arrays operated by light in the visible-wavelength ***,while passive Si_(3)N_(4)-based photonic circuits lend themselves to highly scalable mass production,the integration of low-cost light sources remains a *** this paper,we demonstrate optical biosensors that combine Si_(3)N_(4)sensor circuits with hybrid on-chip organic *** Si_(3)N_(4)-organic hybrid(SiNOH)lasers rely on a dye-doped cladding material that are deposited on top of a passive WG and that are optically pumped by an external light *** of the devices is simple:The underlying Si_(3)N_(4)WGs are structured in a single lithography step,and the organic gain medium is subsequently applied by dispensing,spin-coating,or ink-jet printing processes.A highly parallel read-out of the optical sensor signals is accomplished with a simple *** our proof-of-concept experiment,we demonstrate the viability of the approach by detecting different concentrations of fibrinogen in phosphate-buffered saline solutions with a sensor-length(L-)-related sensitivity of S/L=0.16 rad nM^(-1)mm^(-1).To our knowledge,this is the first demonstration of an integrated optical circuit driven by a co-integrated low-cost organic light *** expect that the versatility of the device concept,the simple operation principle,and the compatibility with cost-efficient mass production will make the concept a highly attractive option for applications in biophotonics and point-of-care diagnostics.

关键词： passive waveguide pumped

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建立市场辅导机制下的“文化保护主义”体系——欧盟影视公共资助模式的思考

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现代传播（中国传媒大学学报） 2007年第2期29卷 108-112页

作者：罗青 Andre lange 中国传媒大学欧洲传媒研究中心欧洲委员会视听传媒观察中心财经市场部

公共资助体系是欧洲国家对以影视为主导的文化创意产业的一系列重要的公共政策,起源于20世纪30年代。世界大战末期的欧洲成为美国电影及美国价值观主导的市场,如何保持欧洲产业的文化多样性和全球竞争力引起了欧洲业界的普遍危机认同。... 详细信息

公共资助体系是欧洲国家对以影视为主导的文化创意产业的一系列重要的公共政策,起源于20世纪30年代。世界大战末期的欧洲成为美国电影及美国价值观主导的市场,如何保持欧洲产业的文化多样性和全球竞争力引起了欧洲业界的普遍危机认同。通过市场和文化的双轨措施实施的公共资助体系,开发出产业之区域整合与互惠模式,创造出一个全新的全球市场竞争力量,保持了欧洲各文化对美国文化强权的抗衡能力的政策模式和产业辅助体系。基于市场竞争和文化多样双重原则下建立起来的视听产业公共资助体系,以“经济和文化”的两手原则为指导,目的是为影视产业的市场化和文化价值服务,其建构的体系原则、资金来源、运营方式均值得尚处于产业化转型,尤其是文化体制改革进程中的中国影视业界借鉴。

关键词：公共资助影视产业文化保护主义欧盟

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Development of an internally braced prosthesis for total talus replacement

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World Journal of Orthopedics 2017年第3期8卷 221-228页

作者： Markus Regauer Mirjam lange Kevin Soldan Steffen Peyerl Sebastian Baumbach Wolfgang Bocker Hans Polzer Klinik für Allgemeine Unfall und Wiederherstellungschirurgie Klinikum der Ludwig-Maximilians-Universitat München Stryker GmbH

Total loss of talus due to trauma or avascular necrosis,for example,still remains to be a major challenge in foot and ankle surgery with severely limited treatment *** of a custom made total talar prosthesis has shown promising results so *** important factors for long time success are degree of congruence of articular surfaces and ligamentous stability of the ***,our aim was to develop an optimized custom made prosthesis for total talus replacement providing a high level of primary stability.A custom made hemiprosthesis was developed using computed tomography and magnetic resonance imaging data of the affected and contralateral talus considering the principles and technology for the development of the ***(Stryker).Additionally,four eyelets for fixation of artificial ligaments were added at the correspondent footprints of the most important *** modifications can be provided according to the clinical requirements:A tri-articular hemiprosthesis or a bi-articular hemiprosthesis combined with the tibial component of the *** ankle replacement system.A feasibility study was performed using a fresh frozen human *** range of motion of the ankle was measured and ligamentous stability was evaluated by use of standard X-rays after application of varus,valgus or sagittal stress with 150 *** implantation of the prosthesis was technically possible via an anterior approach to the ankle and using standard *** osteotomies were not *** ankle dorsiflexion and plantarflexion were measured as 22-0-28 *** anterior displacement of the talus was 6 mm,maximum varus tilt 3 degrees and maximum valgus tilt 2 *** of an internally braced prosthesis for total talus replacement in humans is technically feasible and might be a reasonable procedure in carefully selected cases with no better alternatives left.

关键词： Ankle Avascular necrosis Total loss of talus Prosthesis Hemiprosthesis InternalBrace Talus replacement

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Psychological effects of colorectal cancer screening: Participants vs individuals not invited

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World Journal of Gastroenterology 2016年第43期22卷 9631-9641页

作者： Benedicte Kirk?en Paula Berstad Edoardo Botteri Linn Bernklev Badboni El-Safadi Geir Hoff Thomas de lange Tomm Bernklev Department of bowel cancer screening Cancer Registry of Norway Institute of Clinical Medicine University of Oslo Department of Internal Medicine Vestre Viken Hospital Trust B?rum Hospital Department of Gastroenterology ?stfold Hospital Trust Department of Research and Development Telemark Hospital Trust Department of Health Management and Health Economics Institute of Health and Society University of Oslo

AIM To investigate the possible long-term psychological harm of participating in colorectal cancer(CRC)screening in *** In a prospective, randomized trial, 14294 participants(aged 50-74 years) were invited to either flexible sigmoidoscopy(FS) screening, or a faecal immunochemical test(FIT)(1:1). In total, 4422 screening participants(32%) completed the questionnaire, which consisted of the Hospital Anxiety and Depression Scale and the SF-12, a generic health-related quality of life(HRQOL) measurement, when invited to screening and one year after the invitation. A control group of 7650 individuals was invited to complete the questionnaire only, at baseline and one year after, and 1911(25%) completed the *** Receiving a positive or negative screening result and participating in the two different screening modalities did not cause clinically relevant mean changes in anxiety, depression or HRQOL after one year. FS screening, but not FIT, was associated with an increased probability of being an anxiety case(score ≥ 8) at the one-year follow-up(5.6% of FS participants transitioned from being not anxious to anxious, while 3.0% experienced the reverse). This increase was moderately significantly different from the changes in the control group(in which the corresponding numbers were 4.8% and 4.5%, respectively), P = 0.06. CONCLUSION Most individuals do not experience psychological effects of CRC screening participation after one year, while FS participation is associated with increased anxiety for a smaller group.

关键词： Colorectal cancer screening Flexible sigmoidoscopy Fecal immunochemical test Anxiety Health-related quality of life Control group

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Lifestyle factors in the prevention of coronavirus disease 2019(COVID-19)

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Global Health Journal 2020年第4期4卷 146-152页

作者： Klaus W.lange Yukiko Nakamura Institute of Psychology University of RegensburgRegensburg 93040Germany

Confinement to the home and psychological distress due to the coronavirus disease 2019(COVID-19)pandemicmay lead to harmful health behaviors,such as overeating,sedentary behavior with reduced physical activity,elevated alcohol and tobacco use and increased screen time causing impaired *** of these behaviors areassociated with non-communicable diseases and can interfere with *** no foods,single nutrientsor dietary supplements are capable of preventing infection with COVID-19,a balanced diet containing sufficientamounts of macronutrients and diverse micronutrients is a prerequisite of an optimally functioning ***-energy“Western”diets and obesity are major risk factors for a more severe course of *** use and tobacco also have detrimental effects on the immune ***,population-wide bodyweight control,reduction of smoking rates and limitation of alcohol consumption are important ***,sufficient restorative sleep is needed for adequate immune *** changes in regard to nutrition,exercise,sleep,smoking and alcohol intake may help shift the populationdistribution of infection risk and aid in preventing severe COVID-19 ***-scale surveys should explorethe effects of lifestyle changes,and the provision of reliable lifestyle information and effective interventions toindividuals and communities during the pandemic is a pressing need.

关键词： COVID-19 Prevention Lifestyle Obesity Exercise Sleep

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Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells

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World Journal of Gastroenterology 2018年第2期24卷 170-178页

作者： Peter Wallbaum Sarah Rohde Luise Ehlers Falko lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster Department of MedicineⅡ Division of GastroenterologyRostock University Medical CenterRostock 18057Germany Oscar-langendorff-Institute of Physiology Rostock University Medical CenterRostock 18057Germany Department of Nuclear Medicine Rostock University Medical CenterRostock 18057Germany

AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 *** Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated *** The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in v

关键词： Pancreatic stellate cells Fibrosis Vitamin D2 Vitamin D3 Calcipotriol

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全新世东地中海的陆源碎屑输入及其水文气候变化:海盆尺度地球化学分析

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地学前缘 2022年第4期29卷 156-167页

作者：吴家望姚胜男 Amalia FILIPPIDI 刘志飞 Gert JDE lange 中山大学海洋科学学院广东珠海519082 乌特勒支大学地球科学系荷兰乌特勒支3508TA 同济大学海洋地质国家重点实验室上海200092

地中海位于非洲季风气候和欧洲温带气候的交界处,同时接受周边地区岩石性质和风化状况差异极大的碎屑物质,所以是研究地球表层水文循环的理想区域。前人的认识集中于撒哈拉风尘和尼罗河输入,往往忽视了其他的陆源碎屑沉积,尤其对不同水... 详细信息

地中海位于非洲季风气候和欧洲温带气候的交界处,同时接受周边地区岩石性质和风化状况差异极大的碎屑物质,所以是研究地球表层水文循环的理想区域。前人的认识集中于撒哈拉风尘和尼罗河输入,往往忽视了其他的陆源碎屑沉积,尤其对不同水文气候条件下的潜在变化缺乏考虑。针对形成于全新世非洲湿润期的腐泥层S1页岩沉积,并结合采自岩心顶部的晚全新世/现代沉积物,文章从18个站位选取了30个样品开展碎屑组分的地球化学分析,通过在具有不同干湿环境背景的时间片段上开展盆地尺度的对比(约9.5~8.9 ka vs.约1.7~0 ka),探讨全新世东地中海的陆源碎屑输入模式。Ti/Al、Zr/Al、Ca/Al、Y/Al都清晰显示了经向和纬向上的梯度变化,可用作撒哈拉风尘的可靠指标;这些碎屑元素之间的差异反映了北非风尘来源和传送路径的变化。这些风尘指标在南-北向上的一致变化指示了副热带高压与西风带的交互界限为36°N,该界限在全新世应稳定存在。与风尘相反,河流输入的指标值在早全新世时显著高于现代,并呈现不同的地理分布。K/Al具有西高东低、北高南低的分布特征,指示了地中海北部沿岸地区的河流输入。根据与Ti/Al、K/Al的差异,利用(Cr+Ni)/Al可较好地识别被增强的季风降雨所激活的北非古河流沉积。河流输入在早全新世时显著上升,但不同河流系统的影响范围差异很大,受制于源区水文气候、表层洋流搬运等因素。

关键词：地中海全新世非洲湿润期腐泥层S1 元素地球化学撒哈拉风尘古河流

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Transmission dynamics model and the coronavirus disease 2019 epidemic:applications and challenges

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Medical Review 2022年第1期2卷 89-109页

作者： Jinxing Guan Yang Zhao Yongyue Wei Sipeng Shen Dongfang You Ruyang Zhang Theis lange Feng Chen Departments of Epidemiology and Biostatistics School of Public HealthNanjing Medical UniversityNanjing 211166JiangsuChina China International Cooperation Center for Environment and Human Health Center for Global HealthNanjing Medical UniversityNanjing 211166JiangsuChina Center of Biomedical BigData Nanjing Medical UniversityNanjing 211166JiangsuChina Department of Public Health University of CopenhagenCopenhagenDenmark

Since late 2019,the beginning of coronavirus disease 2019(COVID-19)pandemic,transmission dynamics models have achieved great development and were widely used in predicting and ***,we provided an introduction to the history of disease transmission,summarized transmission dynamics models into three main types:compartment extension,parameter extension and population-stratified extension models,highlight the key contribution of transmission dynamics models in COVID-19 pandemic:estimating epidemiological parameters,predicting the future trend,evaluating the effectiveness of control measures and exploring different possibilities/***,we pointed out the limitations and challenges lie ahead of transmission dynamics models.

关键词： compartment model coronavirus disease 2019 novel coronavirus pneumonia SEIR SIR transmission dynamics model.

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