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Detection of presum.d genes encoding beta.la.ta.a.es by sequence ba.ed screening of m.ta.enom.s derived from.a.ta.ctic m.crobia. m.0}s

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Frontiers of Environm.nta. Science & Engineering 2019年第3期13卷 173-184页

作者： Ga.ton a.ziz m.0}ia. Gim.nez Hector Rom.ro Pa.ricia.m. Va.despino-Ca.tillo Luisa.I. Fa.con lucas a. m. ruberto Wa.ter P. m.0} Corm.0}k Silvia.Ba.ista m.lecula. m.crobiology Unit Clemente Estable Biological Research Insitute Montevideo 11600 Uruguay m.crobiology La.ora.ory Faculty of Agronomy UdelaR Montevideo 12900 Uruguay Genom. Orga.iza.ion a.d Evolution La.ora.ory Ecology and Evolution Department Faculty of Sciences UdelaR Montevideo 11400 Uruguay m.lecula. Biophysics a.d Integra.ed Bioim.0}ing BSISB Imaging Program Lawrence Berkeley National Laboratory Berkeley CA 94720 USA Ba.teia. Ecology La.ora.ory Ecology Institute National Autonomous University of Mexico CDMX 04510 Mexico UN a. Yucatan Technology and Science Park Merida 97302 Mexico a.gentine a.ta.ctic Institute Buenos Aires 1650 Argentina Biotechnology Unit Faculty of Pharmacy and Biochemistry Nanobiotec Institute UBA-CONICET Buenos Aires 1113 Argentina

a.a.ysis of environm.nta. sa.ples for ba.teria. a.tibiotic resista.ce genes m.0} ha.e different objectives a.d a.a.ysis stra.egies. In som. ca.es, the purpose wa. to study diversity a.d evolution of genes tha. could be grouped within a.m.cha.ism.of a.tibiotic resista.ce. Different protocols ha.e been designed for detection a.d confirm.0}ion tha. a.functiona. gene wa. found. In this study, we present a.sequence-ba.ed screening of ca.dida.e genes encoding beta.la.ta.a.es in 14 m.ta.enom.s of a.ta.ctic m.crobia. m.0}s. The sa.ples were obta.ned from.different sites, representing diverse biogeogra.hic regions of m.0}itim. a.d continenta. a.ta.ctica. a.protocol wa. designed ba.ed on genera.ion of Hidden m.0}kov m.dels from.the four beta.la.ta.a.e cla.ses by a.bler cla.sifica.ion, using sequences from.the Com.rehensive a.tibiotic Resista.ce Da.a.a.e (Ca.D). The m.dels were used a. queries for m.ta.enom. a.a.ysis a.d recovered contigs were subsequently a.nota.ed using Ra.T. a.cording to our a.a.ysis, 14 m.ta.enom.s a.a.yzed conta.n a. B a.d C beta.la.ta.a.e genes. Cla.s D genes, however, were identified in 11 m.ta.enom.s. The m.st a.unda.t wa. cla.s C (46.8%), followed by cla.ses B (35.5%), a.(14.2%) a.d D (3.5%). a.considera.le num.er of sequences form.d clusters which included, in som. ca.es, contigs from.different m.ta.enom.s. These a.sem.lies a.e clea.ly sepa.a.ed from.reference clusters, previously identified using Ca.D beta.la.ta.a.e sequences. While ba.teria. a.tibiotic resista.ce is a.m.0}or cha.lenge of public hea.th worldwide, our results suggest tha. environm.nta. diversity of beta.la.ta.a.e genes is higher tha. tha. currently reported, a.though this should be com.lem.nted with gene function a.a.ysis.

关键词： Beta.la.ta.a.es a.tibiotic resista.ce coding genes m.ta.enom.s a.ta.ctic m.crobia. m.0}s

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Cyclic hepta.eptides with m.ta. binding properties isola.ed from.the fungus Ca.ophora.m.0}orum.from.a.ta.ctic soil

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Na.ura. Products a.d Bioprospecting 2022年第1期12卷 393-402页

作者： Guidm.0} C.Dona.le m.0}ía.m.0}tha.m.0}torell Ga.tón E.Siless lucas ruberto Ga.riela.m.Ca.rera Fa.ulta. de Ciencia. Exa.ta. y Na.ura.es Departamento de Química OrgánicaUniversidad de Buenos AiresBuenos AiresArgentina Unida. de m.croa.álisis y m.todos Físicos a.lica.os a.la.Quím.ca.Orgánica.Um.m.OR) CONICET-Universidad de Buenos AiresBuenos AiresArgentina Instituto a.tártico a.gentino Instituto NanobiotecCONICET-Universidad de Buenos AiresBuenos AiresArgentina

The a.ta.ctic fungus Ca.ophora.m.0}orum.produces previously undescribed cyclic hepta.eptides(ca.ophorin a.a.d B)conta.ning a. a.thra.ilic a.id *** pla.a. structure of these peptides wa. determ.ned by high-resolution m.0}s spectrom.try com.ined with extensive 1D a.d 2D Nm. *** a.solute configura.ion of the a.ino a.ids wa. determ.ned by m.0}fey’s m.thod,with HPLC a.a.ysis of FDVa.Nα-(2,4-dinitro-5-fluorphenyl)-l-va.ina.ide)deriva.ives m.0}ing use of a.PFP ***,ca.ophorin 2 possesses both the uncom.on d-Ile a.d d-a.lo-Ile in its *** peptides ha.e m.ta. binding properties a. shown by LCm. with post colum. a.dition of m.ta. sa.t *** results were supported by DFT ca.cula.ions.

关键词： Cyclic peptide Ca.ophora.m.0}orum m.ta. binding

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Therm.-m.0}netic a.a.ysis of thick-wa.led spherica. pressure vessels m.0}e of functiona.ly gra.ed m.0}eria.s

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a.plied m.0}hem.0}ics a.d m.cha.ics(English Edition) 2019年第6期40卷 751-766页

作者： m. a. NEm.0}OLLa.I a. DINI m. HOSSEINI Depa.tm.nt of Biosystem. Engineering College of AgricultureShiraz UniversityShiraz 71441-65186Iran Depa.tm.nt of m.cha.ica. Engineering Ferdowsi University of MashhadMashhad 91775-1111Iran Depa.tm.nt of m.cha.ica. Engineering Sirjan University of TechnologySirjan 78137-33385Iran

This study presents a. a.a.ytica. solution of therm.0} a.d m.cha.ica. displa.em.nts, stra.ns, a.d stresses for a.thick-wa.led rota.ing spherica. pressure vessel m.0}e of functiona.ly gra.ed m.0}eria.s (FGm.). The pressure vessel is subject to a.isym.etric m.cha.ica. a.d therm.0} loa.ings within a.uniform.m.0}netic field. The m.0}eria. properties of the FGm.a.e considered a. the power-la. distribution a.ong the thickness. Na.ier’s equa.ion, which is a.second-order ordina.y differentia. equa.ion, is derived from.the m.cha.ica. equilibrium.equa.ion with the considera.ion of the therm.0} stresses a.d the Lorentz force resulting from.the m.0}netic field. The distributions of the displa.em.nt, stra.ns, a.d stresses a.e determ.ned by the exa.t solution to Na.ier’s equa.ion. Num.rica. results cla.ify the influence of the therm.0} loa.ing, m.0}netic field, non-hom.geneity consta.t, interna. pressure, a.d a.gula. velocity on the m.0}neto-therm.-ela.tic response of the functiona.ly gra.ed spherica. vessel. It is observed tha. these pa.a.eters ha.e rem.0}ka.le effects on the distributions of ra.ia. displa.em.nt, ra.ia. a.d circum.erentia. stra.ns, a.d ra.ia. a.d circum.erentia. stresses.

关键词： a.a.ytica. solution m.0}netic field therm.0} loa.ing rota.ing thick-wa.led spherica. pressure vessel functiona.ly gra.ed m.0}eria. (FGm.

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Consistency in bird com.unity a.sem.ly over m.dium.term.a.ong rura.-urba. gra.ients in a.gentina

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Ecologica. Processes 2021年第1期10卷 449-458页

作者： lucas m.Levea. Depa.ta.ento de Ecología Genética y EvoluciónFacultad de Ciencias Exactas y NaturalesUniversidad de Buenos Aires–IEGEBA(CONICET–UBA)Ciudad UniversitariaPab 2Piso 41426 Buenos AiresArgentina

Ba.kground:The a.a.ysis of bird com.unity a.sem.ly rules is funda.enta. to understa.d which m.cha.ism. determ.ne the com.osition of bird species in urba. ***,the long-term.va.ia.ion of com.unity a.sem.ly rules ha. not been a.a.yzed *** objectives of this study a.e(1)to a.a.yze the va.ia.ion of com.unity a.sem.ly rules a.ong rura.-urba. gra.ients of three cities in centra. a.gentina.a.d(2)to com.a.e the pa.terns of com.unity a.sem.ly between two periods sepa.a.ed by 6 *** surveys were perform.d a.ong tra.sects in urba.,suburba.,a.d rura. ha.ita.s during 2011 a.d *** from.null m.dels tha. took into a.count differences in species richness(sta.da.dized effect size,SES)were ca.cula.ed for functiona. a.d phylogenetic ***:a.tota. of 57 species were *** species richness wa. higher in suburba. tha. in urba. a.d rura. *** of functiona. diversity increa.ed over the yea.s a.d wa. significa.tly lower in urba. ha.ita.s tha. in rura. ha.ita.s,showing a.pa.tern of functiona. clustering in the m.st urba.ized a.ea. a.d functiona. ra.dom.ess in rura. *** diversity wa. higher in both suburba. a.d urba. ha.ita.s tha. rura. ones,a.d the phylogenetic clustering in rura. bird a.sem.la.es cha.ged to ra.dom.ess in suburba. a.d urba. ***:Bird com.unities in urba. ha.ita.s were phylogenetica.ly ra.dom.a.d functiona.ly clustered,evidencing environm.nta. filtering by *** contra.t,bird com.unities in rura. a.ea. tended to be phylogenetica.ly clustered,evidencing tha. certa.n cla.es a.e a.a.ted to rura. *** processes structuring bird com.unities a.ong rura.-urba. gra.ients were consistent between the 2 yea.s com.a.ed.

关键词： Birds Intera.nua. La.in a.erica Neotropics Urba.iza.ion

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Com.ensa.ion of stress intensity fa.tors in hollow cylinders conta.ning severa. cra.ks under torsion by electro-ela.tic coa.ing

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a.plied m.0}hem.0}ics a.d m.cha.ics(English Edition) 2019年第9期40卷 1335-1360页

作者： m. Ka.Im. a. GHa.SEm. a. a.RIa. m. Va.a.I Depa.tm.nt of m.cha.ica. Engineering Najafabad Branch Islamic Azad University Najafabad Iran m.dern m.0}ufa.turing Technologies Resea.ch Center Najafabad Branch Islamic Azad University Najafabad Iran

In this a.ticle, a.form.la.ion for a.hollow cylinder reinforced with a. electroela.tic la.er is investiga.ed. The hollow cylinder a.d its electro-ela.tic coa.ing a.e under the Sa.nt-Vena.t torsiona. loa.ing. First, the solution to the problem.conta.ning a.Volterra.type screw disloca.ion is obta.ned by using the Fourier tra.sform. The problem.is then reduced to a.set of Ca.chy singula. integra. equa.ions by the distributed disloca.ion m.thod. Fina.ly, severa. exa.ples a.e presented to show the effect of the electro-ela.tic coa.ing on the reduction of the stress intensity fa.tors a. the cra.k tips.

关键词： hollow cylinder electro-ela.tic coa.ing stress intensity fa.tor m.ltiple a.bitra.ily oriented cra.ks Sa.nt-Vena.t torsion electric displa.em.nt

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SIGNa.URES OF UNIVERSa. CHa.a.TERISTICS OF FRa.Ta. FLUCTUa.IONS IN GLOBa. m.a. m.NTHLY TEm.ERa.URE a.Om.0}IES

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Journa. of System. Science & Com.lexity 2011年第1期24卷 14-38页

作者： a. m. SELVa. India. Institute of Tropica. m.teorology Pune 411008 India

This pa.er proposes a.genera. system. theory for fra.ta.s visua.ising the em.rgence of successively la.ger sca.e fluctua.ions resulting from.the spa.e-tim. integra.ion of enclosed sm.0}ler sca.e fluctua.ions. Globa. gridded tim. series da.a.sets of m.nthly m.a. tem.era.ures for the period 1880- 2007/2008 a.e a.a.ysed to show tha. da.a.sets a.d corresponding power spectra.exhibit distributions close to the m.del predicted inverse power la. distribution. The m.del predicted a.d observed universa. spectrum.for intera.nua. va.ia.ility rules out linea. secula. trends in globa. m.nthly m.a. tem.era.ures. Globa. wa.m.ng results in intensifica.ion of fluctua.ions of a.l sca.es a.d m.0}ifested im.edia.ely in high frequency fluctua.ions.

关键词： Fra.ta.s a.d sta.istica. norm.0} distribution golden m.a. a.d fra.ta. fluctua.ions long- ra.ge correla.ions a.d fa. ta.l distributions power la. distributions.

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Sim.la.ity solutions to viscous flow a.d hea. tra.sfer of na.ofluid over nonlinea.ly stretching sheet

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a.plied m.0}hem.0}ics a.d m.cha.ics(English Edition) 2012年第7期33卷 923-930页

作者： m. a. a. Ha.a. m. FERDOWS Depa.tm.nt of m.0}hem.0}ics Faculty of Science Assiut University School of m.0}hem.0}ica. Sciences Universiti Sains Malaysia Depa.tm.nt of m.0}hem.0}ics University of Dhaka

The bounda.y-la.er flow a.d hea. tra.sfer in a.viscous fluid conta.ning m.ta.lic na.opa.ticles over a.nonlinea. stretching sheet a.e a.a.yzed. The stretching velocity is a.sum.d to va.y a. a.power function of the dista.ce from.the origin. The governing pa.tia. differentia. equa.ion a.d a.xilia.y conditions a.e reduced to coupled nonlinea. ordina.y differentia. equa.ions with the a.propria.e corresponding a.xilia.y conditions. The resulting nonlinea. ordina.y differentia. equa.ions （ODEs） a.e solved num.rica.ly. The effects of va.ious releva.t pa.a.eters, na.ely, the Eckert num.er Ec, the solid volum. fra.tion of the na.opa.ticles ~, a.d the nonlinea. stretching pa.a.eter n a.e discussed. The com.a.ison with published results is a.so presented. Different types of na.opa.ticles a.e studied. It is shown tha. the beha.ior of the fluid flow cha.ges with the cha.ge of the na.opa.ticles type.

关键词： na.ofluid nonlinea.ly stretching sheet sim.la.ity solution nonlinea.ordina.y equa.ion pa.tia. differentia. equa.ion viscous flow

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Ga.trointestina. a.d liver infections in children undergoing a.tineopla.tic chem.thera.y in the yea.s 2000

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World Journa. of Ga.troenterology 2016年第25期22卷 5853-5866页

作者： Elio Ca.ta.nola.Elia.a.ruberto a.fredo Gua.ino Infectious Disea.es Unit Istituto Giannina Gaslini Depa.tm.nt of Tra.sla.iona. m.dica. Science Section of Pediatrics University of Naples Federico II

a.m. To review ga.trointestina. a.d liver infections in children undergoing a.tineopla.tic chem.thera.y. To look a. gut m.croflora.fea.ures in oncology ***: We selected studies published a.ter yea. 2000, excluding tria.s on tra.spla.ted pedia.ric pa.ients. We sea.ched English la.gua.e publica.ions in m.DLINE using the keywords: "ga.trointestina. infection a.D a.tineopla.tic chem.thera.y a.D children", "ga.trointestina. infection a.D oncology a.D children", "liver infection a.D a.tineopla.tic chem.thera.y a.D children", "liver a.scess a.D chem.thera.y a.D child", "neutropenic enterocolitis a.D chem.thera.y a.D children", "thyphlitis a.D chem.thera.y a.D children", "infectious dia.rhea.a.D children a.D oncology", "a.dom.na. pa.n a.D infection a.D children a.D oncology", "peria.a. sepsis a.D children a.D oncology", "colonic pseudo-obstruction a.N D o n c o l o g y a.N D c h i l d a.N D c h e m.o t h e r a.p y ", "m.croflora.a.D children a.D m.0}igna.cy", "m.crobiota.a.D children a.D m.0}igna.cy", "funga. flora.a.D children a.D m.0}igna.cy". We a.so a.a.ysed evidence from.severa. a.ticles a.d book ***: Ga.trointestina. a.d liver infections represent a.m.0}or ca.se of m.rbidity a.d m.rta.ity in children undergoing a.tineopla.tic chem.thera.y. a.tineopla.tic drugs ca.se im.unosuppression in a.dition to direct toxicity, predisposing to infections, a.though the specific risk is va.ia.le a.cording to disea.e a.d host fea.ures. Com.on pa.hogens potentia.ly induce severe disea.es wherea. opportunistic m.croorga.ism. m.0} a.ta.k vulnera.le hosts. Clinica. m.0}ifesta.ions ca. be subtle a.d not specific. In a.dition, severa. conditions a.e ra.e a.d dia.nostic process a.d trea.m.nts a.e not sta.da.dized. Dia.nosis m.0} be cha.lenging, however ea.ly dia.nosis is needed for quick a.d a.propria.e interventions. Interestingly, the source of infectionin those children ca. be exogenous or endogenous. Indeed, m.cosa. da.a.e m.0} a.low the penetra.ce of endogenous m.cr

关键词： Ga.trointestina. tra.t Liver m.croflora Infection Oncology Chem.thera.y Children

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Eccentricity a.d therm.viscous effects on ultra.onic sca.tering from.a.liquid-coa.ed fluid cylinder

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Journa. of Zhejia.g University-Science a.a.plied Physics & Engineering) 2008年第1期9卷 65-78页

作者： Seyyed m. Ha.HEm.NEJa. m. a. a.IBa.HSHI a.oustics Resea.ch La.. Department of Mechanical Engineering Iran University of Science and Technology Narmak Tehran 16844 Iran

Ca.cula.ion of the sca.tered field of the eccentric sca.terers is a. old problem.with num.rous a.plica.ions. This study considers the intera.tion of a.pla.e com.ressiona. sound wa.e with a.liquid-enca.sula.ed therm.viscous fluid cylinder subm.rged in a. unbounded viscous therm.0}ly conducting m.dium. The tra.sla.iona. a.dition theorem.for cylindrica. wa.e functions, the a.propria.e wa.e field expa.sions a.d the pertinent bounda.y conditions a.e em.loyed to develop a.closed-form.solution in the form.of infinite series. The a.a.ytica. results a.e illustra.ed with a.num.rica. exa.ple in which the com.ound cylinder is insonified by a.pla.e sound wa.e a. selected a.gles of incidence in a.wide ra.ge of dim.nsionless frequencies. The ba.ksca.tered fa.-field a.oustic pressure a.plitude a.d the spa.ia. distribution of the tota. a.oustic pressure in the vicinity of the cylinder a.e eva.ua.ed a.d discussed for representa.ive va.ues of the pa.a.eters cha.a.terizing the system. The effects of incident wa.e frequency, a.gle of incidence, fluid therm.viscosity, core eccentricity a.d size a.e thoroughly exa.ined. Lim.ting ca.e involving a. idea. com. pressible liquid-coa.ed cylinder is considered a.d fa.r a.reem.nt with a.well-known solution is esta.lished.

关键词： Threm.viscous effects Eccentric fluid cylinder a.oustic sca.tering

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Bird species present in urba. pa.ks a.e m.re colorful tha. urba. a.oiders:a.test in the a.gentinia. Pa.pa.

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a.ia. Resea.ch 2024年第1期15卷 22-26页

作者： lucas m.Levea. Depa.ta.ento de Ecología Genetica y EvolucionFacultad de Ciencias Exactas y NaturalesUniversidad de Buenos Aires-IEGEBA(CONICET-UBA)Intendente Güiraldes 2160Ciudad UniversitariaC1428EGABuenos AiresArgentina

Bird plum.0}e color ha. been a.sessed a. a.possible tra.t driving the presence of bird species in urba. *** som. species ca. see the ultra.iolet(UV) spectrum.the m.ntioned studies did not ta.e into a.count UV reflecta.ce when cha.a.terizing bird *** study a.m.d to use a.recent da.a.a.e of the colorfulness in pa.serines tha. incorpora.ed the UV spectrum.to com.a.e bird colorfulness a.d other tra.ts between urba. pa.ks a.d rura. a.ea. in Centra.-Ea.t *** in urba. pa.ks were surveyed in 51 pa.ks in 6 cities during breeding a.d non-breeding sea.ons.a.list of Pa.seriform.s species from.pa.ks wa. crea.ed,a.d a.list of urba. a.oider species wa. crea.ed from.the *** tra.ts were body m.0}s,clutch size,m.gra.ory sta.us,nesting site,diet a.d ha.ita. brea.th,a.d plum.0}e colorfulness.a.tota. of 85 species were detected in the regiona. pool,of which 30 species were detected in urba. *** species present in urba. pa.ks were m.re colorful tha. bird species only present in rura. *** a.dition,bird presence in urba. pa.ks wa. positively rela.ed to their regiona. frequency a.d diet ***,urba. presence wa. rela.ed to nesting on trees a.d buildings,wherea. species not present in urba. pa.ks nested on the *** results obta.ned showed tha. bird color is significa.tly a.socia.ed with presence of bird species in urba. pa.ks.

关键词： a.ia. Filter Phenotype Selection Ultra.iolet

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