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Single-cell profiling of the pig cecum at various developmental stages

Zoological Research 2024年第1期45卷 55-68页

作者： Yan-Yuan Xiao Qing Zhang Fei huang Lin Rao Tian-Xiong Yao Si-Yu Yang Lei Xie Xiao-Xiao Zou Li-Ping Cai Jia-Wen Yang Bin Yang lu-sheng huang National Key Laboratory for Swine Genetic Improvement and Germplasm Innovation Ministry of Science and Technology of ChinaJiangxi Agricultural UniversityNanchangJiangxi 330045China

The gastrointestinal tract is essential for food digestion,nutrient absorption,waste elimination,and microbial ***-cell transcriptome profiling of the intestinal tract has greatly enriched our understanding of cellular diversity,functional heterogeneity,and their importance in intestinal tract development and *** such profiling has been extensively conducted in humans and mice,the single-cell gene expression landscape of the pig cecum remains ***,single-cell RNA sequencing was performed on 45572 cells obtained from seven cecal samples in pigs at four different developmental stages(days(D)30,42,150,and 730).Analysis revealed 12 major cell types and 38 subtypes,as well as their distinctive genes,transcription factors,and regulons,many of which were conserved in *** increase in the relative proportions of CD8^(+)T and Granzyme A(low expression)natural killer T cells(GZMA^(low)NKT)cells and a decrease in the relative proportions of epithelial stem cells,Tregs,RHEX^(+)T cells,and plasmacytoid dendritic cells(pDCs)were noted across the developmental ***,the post-weaning period exhibited an up-regulation in mitochondrial genes,COX2 and ND2,as well as genes involved in immune activation in multiple cell ***-cell crosstalk analysis indicated that IBP6^(+)fibroblasts were the main signal senders at D30,whereas IBP6^(−)fibroblasts assumed this role at the other *** cells established interactions with epithelial cells and IBP6^(+)fibroblasts in the D730 cecum through mediation of GZMA-F2RL1/F2RL2 *** study provides valuable insights into cellular heterogeneity and function in the pig cecum at different development stages.

关键词： Single-cell RNA-seq Cecum Bama Xiang pigs Various developmental stages Cellular heterogeneity

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Pig H3K4me3,H3K27ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

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Zoological Research 2024年第1期45卷 138-151页

作者： Tao Jiang Zhi-Min Zhou Zi-Qi Ling Qing Zhang Zhong-Zi Wu Jia-Wen Yang Si-Yu Yang Bin Yang lu-sheng huang National Key Laboratory of Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely *** serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated ***,we conducted an integrated analysis using H3K27ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult *** aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental *** on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity,results indicated that H3K27ac exhibited stronger associations with gene expression than ***,1.45%of TEs overlapped with either the H3K27ac or H3K4me3 peaks,with the majority displaying tissue-specific ***,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K27ac peaks of the adult and fetal ***-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific *** note,1967 TE-containing transcripts were enriched in the *** identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2(a cell cycle-related protein kinase)in our pig *** element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary ***,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive

关键词： Transposable elements Porcine Histone modification Alternative promoter TE-containing transcript

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Single-cell profiling of the pig cecum at various developmental stages

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Zoological Research 2024年第1期

作者： Yan-Yuan Xiao Qing Zhang Fei huang Lin Rao Tian-Xiong Yao Si-Yu Yang Lei Xie Xiao-Xiao Zou Li-Ping Cai Jia-Wen Yang Bin Yang lu-sheng huang

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Pig H3K4me3, H3K27ac, and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

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Zoological Research 2024年第1期

作者： Tao Jiang Zhi-Min Zhou Zi-Qi Ling Qing Zhang Zhong-Zi Wu Jia-Wen Yang Si-Yu Yang Bin Yang lu-sheng huang

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Scan of the endogenous retrovirus sequences across the swine genome and survey of their copy number variation and sequence diversity among various Chinese and Western pig breeds

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Zoological Research 2022年第3期43卷 423-441页

作者： Jia-Qi Chen Ming-Peng Zhang Xin-Kai Tong Jing-Quan Li Zhou Zhang Fei huang Hui-Peng Du Meng Zhou Hua-Shui Ai lu-sheng huang State Key Laboratory for Swine Genetic Improvement and Production Technology Jiangxi Agricultural UniversityNanchangJiangxi 330045China

In pig-to-human xenotransplantation,the transmission risk of porcine endogenous retroviruses(PERVs)is of great ***,the distribution of PERVs in pig genomes,their genetic variation among Eurasian pigs,and their evolutionary history remain *** scanned PERVs in the current pig reference genome(assembly Build 11.1),and identified 36 long complete or near-complete PERVs(lc PERVs)and 23 short incomplete PERVs(si PERVs).Besides three known PERVs(PERV-A,-B,and-C),four novel types(PERV-JX1,-JX2,-JX3,and-JX4)were detected in this *** to evolutionary analyses,the newly discovered PERVs were more ancient,and PERV-Bs probably experienced a bottleneck~0.5 million years ago(Ma).By analyzing63 high-quality porcine whole-genome resequencing data,we found that the PERV copy numbers in Chinese pigs were lower(32.0±4.0)than in Western pigs(49.1±6.5).Additionally,the PERV sequence diversity was lower in Chinese pigs than in Western *** the lc PERV copy numbers,PERV-A and-JX2 in Western pigs were higher than in Chinese ***,Bama Xiang(BMX)pigs had the lowest PERV copy number(27.8±5.1),and a BMX individual had no PERV-C and the lowest PERV copy number(23),suggesting that BMX pigs were more suitable for screening and/or modification as xenograft ***,we identified 451 PERV transposon insertion polymorphisms(TIPs),of which 86 were shared by all 10 Chinese and Western pig *** findings provide systematic insights into the genomic distribution,variation,evolution,and possible biological function of PERVs.

关键词： PERVs Chinese and Western pigs Copy number variation Evolutionary history Biological function prediction

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High-Volume Mineral Admixtures Cement: The Effects of Particle Size Distribution

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Journal of Wuhan University of Technology(Materials Science) 2024年第1期39卷 102-108页

作者：黄启珉 WANG Kun lu Jiping YU Jianping sheng Zhenhua 杨露 State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China Fujian Jinniu Cement Co. LtdSanming 353300China

The effects of high-volume slag-fly ash cement with different particle sizes on hydration degree,microstructure and mechanical properties were systematically studied,by means of laser particle size(DLS),X-ray diffraction (XRD),comprehensive thermal analysis (TG-DTA),scanning electron microscopy(SEM) and mechanical properties *** results show that suitable particle size distribution of cementitious material has significantly promoting effects on hydration reaction rate and mechanical *** with slag without further grinding,the slag after ball milling for 4 h has an obvious improvement in reactivity,which also provides a faster hydration rate and higher compressive strength for the cementitious *** the slag milled for 1 and 4 h is mixed at a mass ratio of 2:1 (i e,slag with D_(50) of 7.4μm and average size of 9.9μm,and slag with D_(50) value of 2.6μm and average size of 5.3μm),and a certain amount of fly ash is added in,the most obvious improvement of compressive strength of cement is achieved.

关键词： ultrafine slag microstructure compressive strength particle size

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Reputation-based joint optimization of user satisfaction and resource utilization in a computing force network

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Frontiers of Information Technology & Electronic Engineering 2024年第5期25卷 685-700页

作者： Yuexia FU Jing WANG lu lu Qinqin TANG sheng ZHANG China Mobile Research Institute Beijing 100053China Purple Mountain Laboratories Nanjing 211111China China Mobile Communications Corporation Beijing 100033China

Under the development of computing and network convergence,considering the computing and network resources of multiple providers as a whole in a computing force network(CFN)has gradually become a new ***,since each computing and network resource provider(CNRP)considers only its own interest and competes with other CNRPs,introducing multiple CNRPs will result in a lack of trust and difficulty in unified *** addition,concurrent users have different requirements,so there is an urgent need to study how to optimally match users and CNRPs on a many-to-many basis,to improve user satisfaction and ensure the utilization of limited *** this paper,we adopt a reputation model based on the beta distribution function to measure the credibility of CNRPs and propose a performance-based reputation update ***,we formalize the problem into a constrained multi-objective optimization problem and find feasible solutions using a modified fast and elitist non-dominated sorting genetic algorithm(NSGA-II).We conduct extensive simulations to evaluate the proposed *** results demonstrate that the proposed model and the problem formulation are valid,and the NSGA-II is effective and can find the Pareto set of CFN,which increases user satisfaction and resource ***,a set of solutions provided by the Pareto set give us more choices of the many-to-many matching of users and CNRPs according to the actual situation.

关键词： Computing force network Resource scheduling Performance-based reputation User satisfaction

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Hydrodynamics and OCM reaction performance in a bubbling fluidized bed reactor and a riser reactor

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Particuology 2024年第3期86卷 188-204页

作者： Min Cai Shuangzhu Kong sheng Chen Mengxi Liu Chunxi lu State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing102249China China Special Equipment Inspection and Research Institute Beijing102249China

The reasonable reactor design is of great importance for increasing the C_(2) yield(C2H4 and C2H6)of the oxidative coupling of methane(OCM),and the OCM reactor should remove the heat released in reactions quickly and efficiently and minimize the consecutive reaction of ethylene to carbon *** fluidized bed reactor is characterized by excellent heat transfer,superior mass transport,and large handling capacity,while fewer studies focused on large-scale fluidized bed reactors for the OCM ***,large cold-model experiments and computational fluid dynamics simulations were conducted to investigate hydrodynamics and the OCM reaction performance in a large-scale bubbling fluidized bed(BFB)and a large-scale *** the BFB reactor,consecutive reactions of ethylene are acute because of the strong gas back-mixing,high solids holdup,and non-uniform solids *** the consecutive reactions of ethylene are negligible due to the plug flow structure and low solids holdup in the riser ***,both reactors can achieve isothermal operation for the OCM *** C_(2) selectivity of 45.4% and C_(2) yield of 21.1% are obtained in the riser reactor,increasing by 20.3% and 5.8% individually than that in the BFB *** study provides useful information and reference to the OCM reactor designandcommercialization.

关键词： Oxidative coupling of methane Bubbling fluidized bed reactor Riser reactor Hydrodynamics Isothermal operation

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Revealing the Structure-Property Difference of Natural Rubber Prepared by Different Methods: Protein and Gel Content are Key Factors

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Chinese Journal of Polymer Science 2024年第4期42卷 457-467页

作者： Shao-Qi huang Jun-Qi Zhang Yong Zhu Ling-Min Kong lu-sheng Liao Fu-Quan Zhang Zheng-Tian Xie Jin-Rong Wu State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu610065China Hainan Provincial Key Laboratory of Natural Rubber Processing Agricultural Products Processing Research Institute of Chinese Academy of Tropical Agricultural SciencesZhanjiang524001China

Natural rubber (NR) is widely used in various fields including aerospace, military industry and transportation due to its superior elasticity and comprehensive mechanical properties. Nonetheless, the commercial NR prepared by different methods usually exhibits different mechanical properties, primarily due to variations in processing conditions during the conversion from latex to bulk rubber material. Consequently, this poses challenges in scientific research and industrial production of NR. In order to assess the properties of various commercially available NR and identify key structural and compositional components, this study systematically compares and analyzes four representative NR raw materials: air dried sheet (ADS), ribbed smoked sheets (RSS), constant viscidity NR (CV), and whole field latex rubber (WF). The investigation focuses on evaluating their static mechanical behavior, SIC behavior, wear resistance, and fatigue resistance. The findings indicate that protein and gel content exhibit a crucial influence on the NR properties. These constituents contribute to the formation of a high-crosslinking density region, generating a heterogeneous network structure within the rubber. This structure amplifies strains during deformation, leading to earlier and stronger strain-induced crystallization (SIC). Among the four commercial NR brands, RSS demonstrates superior overall mechanical and dynamic properties owing to its high protein and gel content. This study serves as a valuable reference for comprehending the differences in properties among various commercial NR, thereby offering guidance for the actual processing and selection of NR.

关键词： Natural rubber Processingmethod Protein Gel content Property

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Origin and evolution of false brinelling and fretting corrosion under rolling conditions

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Friction 2024年第4期12卷 618-631页

作者： Xin JIA lu huang Miao SUN Wenbin LI National Institute of Metrology Beijing 100029China Yantai Tiancheng Machinery Co. Ltd.Yantai 264006China

Rolling bearings working in oscillating applications often suffer from false brinelling and fretting corrosion that limit their service *** present,the study of oscillation-induced failures is primarily dominated by a large number of sliding and bench ***,most of the previous work are not able to fully describe the two closely related but different failure *** this study,the origin and evolution of the two failures are thoroughly investigated under strictly controlled oscillating rolling *** results show that failure evolves from false brinelling to fretting corrosion under dry friction conditions regardless of the oscillation *** process is accompanied by a large area peeling and serious oxidation,and the oxygen content increases from 2.50 wt%to 21.22 wt%.Oxidation is a crucial factor in the evolution of false brinelling into fretting ***,the distribution of microwear and oxygen content depend on the oscillation *** lubrication conditions,the friction coefficient(COF)is smaller in the initial stage and led to local slip or transient instability of rolling ***,the size of the wear marks and COF increased and appeared to be larger than those under a dry friction ***,grease can separate the contact surfaces,only surface deformation owing to false brinelling and slight damage at the roughness level *** evolution of false brinelling and fretting corrosion is also closely related to residual *** brinelling often occurrs with residual stress accomulation,meanwhile,the residual stress increases from 120 to 300 *** peeling occurs,residual stress is *** failure pattern transformed from false brinelling to fretting corrosion and is accompanied by *** far,none of investigation is able to show satisfactory evolution of oscillating-induced failures,this study may contribute to more scientific understanding of rolling bearings against long-

关键词： oscillating false brinelling fretting corrosion wear residual stress

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