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Genome assembly of autotetraploid Actinidia arguta highlights adaptive evolution and enables dissection of important economic traits

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Plant Communications 2024年第6期5卷 173-192页

作者： Xue-mei lu Xiao-Fen Yu Guo-Qiang Li Ming-Hao Qu Huan Wang Chuang Liu Yu-Ping Man Xiao-Han Jiang Mu-Zi Li Jian Wang Qi-Qi Chen Rui Lei Cheng-Cheng Zhao Yun-Qiu Zhou Zheng-Wang Jiang Zuo-Zhou Li Shang Zheng Chang Dong Bai-Lin Wang Yan-Xiang Sun Hui-Qin Zhang Jie-Wei Li Quan-Hui Mo Ying Zhang Xin Lou Hai-Xu Peng Ya-Ting Yi He-Xin Wang Xiu-Jun Zhang Yi-Bo Wang Dan Wang Li Li Qiong Zhang Wen-Xia Wang Yongbo Liu Lei gao Jin-Hu Wu Yan-Chang Wang CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhanHubeiChina Key Laboratory of Western China’s Environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhouGansuChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina Wuhan Frasergen Bioinformatics Co. LtdWuhanHubeiChina Institute of Soil and Fertilizer Anhui Academy of Agricultural SciencesHefeiChina College of Agricultural Sciences Xichang UniversityXichangSichuanChina Department of Horticulture Heilongjiang Academy of Agricultural SciencesHarbinHeilongjiangChina College of Life Sciences Langfang Normal UniversityLangfangHebeiChina Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhouZhejiangChina Guangxi Institute of Botany Chinese Academy of SciencesGuilinGuangxiChina Xi’an Botanical Garden of Shaanxi Province Institute of Botany of Shaanxi ProvinceXi’anShaanxiChina Institute of Modern Agricultural Research Dalian UniversityDalianLiaoningChina Bioinformatics Center College of Plant Science and TechnologyBeijing University of AgricultureBeijingChina College of Agriculture Eastern Liaoning UniversityDandongLiaoningChina Dalian Institute of Chemical Physics Chinese Academy of SciencesDalianLiaoningChina State Environmental Protection Key Laboratory of Regional Eco-process and Function Assessment Chinese Research Academy of Environmental SciencesBeijingChina Hubei Hongshan Laboratory WuhanHubeiChina The New Zealand Institute for Plant and Food Research Limited AucklandNew Zealand

Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit,rapid softening,and excellent cold *** evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still ***,we generated a chromosome-scale genome assembly of an autotetraploid male *** *** genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 *** and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups,Southern and Northern,which first diverged 12.9 million years *** underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late *** genomics studies using paleoclimate data enabled us to discern the evolution of the species’adaptation to different historical *** genes(AaCEL1,AaPME1,and AaDOF1)related to flesh softening were identified by multi-omics analysis,and their ability to accelerate flesh softening was verified through transient expression assays.A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome(Chr3)or autosomal chromosomes showed biased expression during stamen or carpel *** chromosome-level assembly of the autotetraploid *** genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of ***.

关键词： kiwiberry dioecism adaptive evolution paleoclimate GEA rapid softening

Spatial and temporal variations in nitrogen retention effects in a subtropical mountainous basin in Southeast China

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Journal of Mountain Science 2021年第10期18卷 2672-2687页

作者： LIU mei-bing CHEN Xing-wei CHEN Ying gao lu DENG Hai-jun State Key Laboratory for Subtropical Mountain Ecology of the Ministry of Science and Technology and Fujian Province Fujian Normal UniversityFuzhou 350007China College of Geographical Sciences Fujian Normal UniversityFuzhou 350007China Fujian Provincial Engineering Research Center for Monitoring and Assessing Terrestrial Disasters Fujian Normal UniversityFuzhou 350007China

Nitrogen retention within a watershed reduces the amount of N exported to the ocean;however, it worsens environmental problems, including surface water eutrophication, aquifer pollution, acid rain, and soil acidification. Here, we adopted the Soil and Water Assessment Tool(SWAT) model to describe the riverine N output and retention effects in the Shanmei Reservoir Basin, a subtropical mountainous basin located in Quanzhou City, Southeast China. The results revealed that farmlands and orchards in the upstream and central parts of the basin were the dominant land use types, which contributed large N yields. Fertilizer application was the key source of riverine N output and N retention within the basin. On average, approximately 64% of anthropogenic N inputs were retained within the basin, whereas 36% of total N was exported to the downstream and coastal areas. The average N retention efficiency was 80% in a dry year, and within the year, N retention occurred in spring and summer and N release occurred in autumn and winter. The annual variation in N retention within the basin was largely dominated by changes in rainfall and runoff, whereas the seasonal characteristics of N retention were mainly affected by fertilization. Even with a large decrease in fertilizer application, owing to the contributions of the residual N pool and river background, the riverine N output still maintained a certain base value. The effects of precipitation, land use types, and agricultural fertilizer on N retention should be comprehensively considered to implement reasonable N management measures.

关键词： Nitrogen retention Riverine nitrogen output Precipitation and runoff Fertilization management Subtropical mountainous basin

Zn/ETS-10催化CO_(2)和甘油高选择性生成碳酸甘油酯的研究

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现代化工 2024年第8期44卷 104-109页

作者：蒋夫花高张玺陈佳瑶于露向梅吴泽颖常州工学院化工与材料学院江苏常州213032 常州大学石油化工学院江苏常州213164

采用优化工艺合成了高结晶度的ETS-10沸石,进一步通过湿法浸渍将系列过渡金属负载于ETS-10沸石上制得系列催化剂。考察了系列催化剂对甘油羰基化反应的催化性能。结果表明,Zn/ETS-10可催化该反应并高选择性地生成碳酸甘油酯。在优化的... 详细信息

采用优化工艺合成了高结晶度的ETS-10沸石,进一步通过湿法浸渍将系列过渡金属负载于ETS-10沸石上制得系列催化剂。考察了系列催化剂对甘油羰基化反应的催化性能。结果表明,Zn/ETS-10可催化该反应并高选择性地生成碳酸甘油酯。在优化的工艺条件和Zn/ETS-10催化作用下,甘油转化率可达34.55%,碳酸甘油酯的选择性高达56.34%。此外,Zn/ETS-10经过8次反应循环后仍保持较高的催化活性和显著的稳定性。

关键词： CO_(2) 甘油碳酸甘油酯 ETS-10沸石锌

NνDEx-100 conceptual design report

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Nuclear Science and Techniques 2024年第1期35卷 18-33页

作者： Xi-Guang Cao Yan-Long Chang Kai Chen Emilio Ciuffoli Li-Min Duan Dong-Liang Fang Chao-Song gao Surja K.Ghorui Peng-Chong Hu Qiang Hu Si-Yuan Huang Ze-Yu Huang Lei Lang Yu-Lan Li Zhi-Jie Li Tian-Yu Liang Jun Liu Chen-Gui lu Fa-Tai Mai Yuan mei Hao Qiu Xiang-Ming Sun Xiao-Xing Tang Hu-Lin Wang Qian-Ming Wang Le Xiao Mu-Yun Xiao Jian-Yu Xin Nu Xu Peng Yang Yi-Chen Yang Zhen Yang Zong-Yang Yu Dong-Liang Zhang Jun-Wei Zhang Cheng-Xin Zhao Dou Zhu NνDEx-100 collaboration Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Lanzhou University Lanzhou 730000China Central China Normal University Wuhan 430079China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Lawrence Berkeley National Laboratory BerkeleyCA 94720USA North China University of Water Resources and Electric Power Zhengzhou 450045China 不详

Observing nuclear neutrinoless double beta (0vββ) decay would be a revolutionary result in particle *** such a decay would prove that the neutrinos are their own antiparticles,help to study the absolute mass of neutrinos,explore the origin of their mass,and may explain the matter-antimatter asymmetry in our universe by lepton number *** propose developing a time projection chamber (TPC) using high-pressure ^(82)SeF_(6) gas and Topmetal silicon sensors for readout in the China Jinping Underground Laboratory (CJPL) to search for neutrinoless double beta decay of82Se,called the NvDEx *** being located at CJPL with the world’s thickest rock shielding,NvDEx combines the advantages of the high Qββ(2.996 MeV) of82Se and the TPC’s ability to distinguish signal and background events using their different topological *** makes NvDEx unique,with great potential for low-background and high-sensitivity 0 vββ***-100,a NvDEx experiment phase with 100 kg of SeF_(6)gas,is being built,with plans to complete installation at CJPL by *** report introduces 0 vββ physics,the NvDEx concept and its advantages,and the schematic design of NvDEx-100,its subsystems,and background and sensitivity estimation.

关键词： Neutrinoless double beta decay Time projection chamber ^(82)SeF_(6) China Jinping Underground Laboratory

Performance of Three Adelphocoris spp.(Hemiptera: Miridae) on Flowering and Non-flowering Cotton and Alfalfa

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Journal of Integrative Agriculture 2014年第8期13卷 1727-1735页

作者： gao Zhen PAN Hong-sheng LIU Bing lu Yan-hui LIANG Ge-mei State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

The genus Adelphocoris（Hemiptera： Miridae） is a group of key insect pests in cotton fields in China that includes three dominant species： A. suturalis（Jakovlev）, A. lineolatus（Goeze） and A. fasciaticollis（Reuter）. Previous field surveys have found that adults of these Adelphocoris species usually move onto specific host plants when the plants enter the flowering stage. To determine the potential trade-offs for this host-plant preference behavior, the performance of these three Adelphocoris spp. on flowering and non-flowering cotton and alfalfa were compared in the laboratory. The results showed that Adelphocoris spp. had significantly higher nymphal developmental and survival rates, along with increased adult longevity and fecundity on flowering cotton and alfalfa than on non-flowering plants of either species. In addition, compared with cotton plants, alfalfa generally promoted better performance of these three Adelphocoris species, especially for A. lineolatus. Simple correlation analysis indicated that female adult longevity was positively correlated to male adult longevity and female fecundity, and female fecundity was positively correlated to nymphal development and survival rates. This study demonstrated a positive correlation between adult preference and offspring/adult performance for all three Adelphocoris species, with no evidence of any trade-offs for this preference for flowering host plants. These findings support the hypothesis that hemimetabolous insects such as mirid bugs have positive adult preference-adult/nymphal performance relationships, which is likely due to the similar feeding habits and nutritional requirements of adults and nymphs.

关键词： Adelphocoris lineolatus Adelphocoris suturalis Adelphocoris fasciaticollis preference-performance relationship cotton alfalfa flowering stage

Prevalence of Epstein-Barr virus infection and characteristics of lymphocyte subsets in newly onset juvenile dermatomyositis

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World Journal of Pediatrics 2021年第2期17卷 205-209页

作者： Qi Zheng Kun Zhu Cai-Na gao Yi-Ping Xu mei-Ping lu Department of Rheumatology Immunology and AllergyChildren’s HospitalZhejiang University School of Medicine57#Zhu Gan RoadHangzhou 310000China Department of Pathology Children's HospitalZhejiang University School of MedicineHangzhouChina

Background The underlying etiology of juvenile dermatomyositis(JDM)is unknown.T cell deficiency as well as Epstein-Barr virus(EBV)infection had been suspected to be involved in the pathogenesis,but it has been poorly evaluated in JDM *** This study described the traits of T and B lymphocyte subsets in newly onset JDM patients and the incidence of EBV infection in JDM patients compared with match *** developed JDM patients from 2014 to 2018 were included in the *** with different markers(CD3+,CD3+CD4+,CD3+CD8+,CD3-CD19+and CD3-CD16+CD56+)were tested with flow cytometry in the first admission or after 6 months of *** analysis was conducted to compare the EBV infection in the group of JDM patients and *** We observed that JDM patients had higher positive rate of Epstein-Barr nuclear antigen-immunoglobulin G(IgG)(Plusions The level of CD3-CD16+CD56+cells may associate with the clinical course of *** may act as an environmental factor predisposing patients to the development of JDM.

关键词： Epstein-barr virus Juvenile dermatomyositis Lymphocytes

bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis

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aBIOTECH 2022年第1期3卷 1-11页

作者： Juan gao mei-ing Wang Jing-Jing Wang Hai-Ping lu Jian-Xiang Liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

High temperature elicits a well-conserved response called the unfol ded protein response(UPR)to bring protein homeostasis in the endoplasmic reticulum(ER).Two key UPR regulators bZIP28 and bZIP60 have been shown to be essential for maintaining fertility under heat stress conditions in Arabidopsis,however,the function of transcriptional activator bZIP17,a paralog of bZIP28,in heat stress response at reproductive stage is not *** we found that bzip17 mutant plants were sensitive to heat stress in terms of silique length and fertility comparing to that of wildtype(WT)Arabidopsis plants,and transcri ptomic analysis showed that 1380 genes were specifically up-regulated and 493 genes were specifically down-regulated by heat stress in the flowers of WT plants comparing to that in bzip17 mutant *** bZIP17-dependent up-regulated genes were enriched in responses to a biotic stresses such as water deprivation and salt *** chromatin immuno-preci pitation coupled with high-throughput sequencing(ChIP-Seq)uncovered 1645 genes that were direct targets of bZIP17 in MYC-bZIP17 expressing seedlings subjected to heat *** these 1645 genes,ERSE-II cis-element was enriched in the binding peaks of their promoters,and the up-regulation of 113 genes by heat stress in flowers was dependent on *** results revealed direct targets of bZIP17 in flowers during heat stress responses and demonstrated the important role of bZIP17 in maintai ning fertility upon heat stress in plants.

关键词： Arabidopsis bZIP17 Flower Fertility Heat Stress UPR

Identifying crack initiation stress threshold in brittle rocks using axial strain stiffness characteristics

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Journal of Mountain Science 2018年第6期15卷 1371-1382页

作者： gao mei-ben LI Tian-bin MENG lu-bo MA Chun-chi XING Hui-lin state key laboratory of geohazard prevention and geoenvironment protection chengdu university of technologyChengdu 610059China college of environment and civil engineering chengdu university of technologyChengdu 610059China centre for geoscience computing school of earth sciencesthe university of queenslandSt Lucia.QLD 4072Australia

As an estimate for the in-situ spalling strength around massive underground excavations to moderately jointed brittle rocks, crack initiation stress marks the initiation of rock micro fracturing. It is crucial to accurately identify crack initiation stress level by proper method. In this study, confined compression tests of sandstone samples are used to examine the validity/applicability of proposed axial strain stiffness method. The results show that by highlighting the minuscule changes in stress-strain curve, the axial strain stiffness curve provided further insight into rock failure process and revealed five stages:(a) irregular fluctuation,(b) nearly horizontal regular fluctuation,(c) irregular fluctuation gradually decreasing to zero,(d) extreme fluctuation, and(e) near zero, which mainly correspond to five stages of stress–strain curve. The ratio of crack-initiation stress to peak strength determined using this approach is 0.44–0.51, similar to the ranges previously reported by other researchers. In this method, the key is to accurately detect the end point of the stage(b), "nearly horizontal regular fluctuation" characterized by a sudden change in axial strain stiffness curve, and the sudden change signifies crack initiation in rock sample. Finally, the research indicates that the axial strain stiffness curve can provide a mean to identify the crack-initiation stress thresholds in brittle rocks.

关键词： Brittle rock fracture Rock failure Sandstone Crack initiation stress Axial strain-stiffness Triaxial test

经济合作与发展组织国家托育政策目标:价值取向、发展特点及经验启示

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幼儿教育 2024年第15期 52-55页

作者：冯江英张眉高雅孔露赵勇宋映泉新疆师范大学教育科学学院新疆乌鲁木齐830017 北京大学中国教育财政科学研究所北京100091

经济合作与发展组织国家托育政策目标包括支持儿童身心健康发展、提高人口生育率、推动女性就业、增加家庭福利、促进经济发展、保障社会公平正义等,其托育政策目标整体上呈现出从单一目标走向多元融合、从工具性价值转向本体性价值的... 详细信息

经济合作与发展组织国家托育政策目标包括支持儿童身心健康发展、提高人口生育率、推动女性就业、增加家庭福利、促进经济发展、保障社会公平正义等,其托育政策目标整体上呈现出从单一目标走向多元融合、从工具性价值转向本体性价值的发展特点。经济合作与发展组织国家托育政策目标的演变对推动我国托育政策的发展具有一定启示意义。

关键词：经济合作与发展组织托育政策目标多元整合儿童权利优先价值取向