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Actin and myosin during pollen germination

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Chinese Science Bulletin 1998年第8期43卷 690-694页

作者： Dongtao Ren Shengcheng Han longfei yan Chinese Agr Univ Coll Biol Sci Beijing 100094 Peoples R China

Actin and myosin from pollen tubes of Lilium davidii were studied by using immunoblotting, Dot_Blot and myosin Ca 2+_ATPase analysis. On immunoblotting of the total soluble pollen tube proteins, anti_α_actin antibody labelled a polypeptide approximately 43 ku, which is considered to be the actin of lily. The mRNA encoding actin in ungerminated pollen and germinated pollen were both undetectable in our experiments. A myosin exhibited Ca 2+_ATPase activity, with a native molecular weight of 460 ku has been identified by using immunoblotting. A polypeptide of about 205 ku and a polypeptide of about 20 ku were the heavy chain and a set of light chain of the myosin, which can crossreact with anti_skeletal muscle myosin heavy chain monoclonal antibody and anti_skeletal muscle myosin light chain (20 ku) monoclonal antibody, respectively. The Ca 2+_ATPase activities of myosin in crude extracts of germinated pollen were positively related to the growth rates of pollen tubes.

关键词： pollen of lily actin myosin.

Expression of Chlamydomonas actin-gfp fusion gene in tobacco suspension cell and polymerization of the actin-gfp protein in vitro

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Chinese Science Bulletin 2001年第10期46卷 806-811页

作者： yanG Yi LIU Guoqin yan longfei College of Biological Sciences China Agricultural UniversityBeijing 100094China Laboratory of Plant Physiology and Biochemistry Ministry of AgricultureChina

The fusion gene of actin (cDNA of Chlamydo-monas reinhardtii) and green fluorescence protein (gfp) had been constructed into two expression vectors which could be expressed in E. coli and tobacco suspension cells BY2. The correct expression was observed in E. coli and BY2 with a fluorescence microscopy. The fusion protein, which took part in the membrane skeleton, was mainly located peripherally along the membrane, specially the fusion protein was distributed around nucleus and cell plate, while the fusion protein also forms F-actin in the cell. The fusion protein was purified from B121plus by ammonium sulfate fractionation, ion exchange chromatography and hydrophobic interaction chromatography. The purified production could polymerize into F-actin when the actin polymerizing buffer was *** was demonstrated that the characteristics and function of actin in Chlamydomonas was similar with those of animals and higher plants.

关键词： actin gfp prokaryotic expression eukaryotic expression.

From pollen actin to crop male sterility

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Chinese Science Bulletin 2000年第9期45卷 784-788页

作者： yan longfei LIU Guoqin XIAO Xingguo Laboratory of Plant Physiology and Biochomistry Ministry of AgriultureCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China

Actin plays an important role in the life activity of animal and plant cells. Pollen cells have plenty of actin whose structure and characteristics are very similar to the animal actin. The nucleotide sequence and amino acid sequence of plant actin gene are very similar to those of the animal gene. The content of pollen actin from male sterile plants is much more lower than that from its maintainer plants. The expression of actin gene is organ-specific during the plant development. The expression quantity of actin gene in pollen is much more higher than those from root, stem and leaf. The expression plasmid of the anti-sense actin gene was constructed, transferred to the protoplasts of wheat and tomato to inhibit the expression of actin gene in pollen and thus the male sterile plants of wheat and tomato were obtained. The actin in pollens from the transgenic plants was reduced significantly, whereas the pistil was not affected. This study might pave a new way to breeding male sterile lines for the

关键词： actin pollen male sterility.

Membrane skeleton spectrin in pollen and pollen tube

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Chinese Science Bulletin 1999年第10期44卷 930-933,961页

作者： yan Li longfei yan S. Y. Zee B. Q. Huang China Agr Univ Coll Biol Sci Beijing 100094 Peoples R China Univ Hong Kong Dept Bot Hong Kong Peoples R China

Immuno-western blots, immunofluorescence and immuno-gold labeling methods were used to study the existence and distribution of a homologue of the membrane skeleton spectrin in the pollen and pollen tube of Lilium davidii Duch. A spectrin homologue was found to exist in the pollen and pollen tube, distributed on membranes of secretory vesicles near Golgi apparatus.

关键词： membrane skeleton spectrin immuno-blots and label pollen plant cell.

低温等离子体活化水与介质阻挡放电联合处理对草莓冷杀菌效果及品质的影响

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食品科学 2022年第17期43卷 105-116页

作者：赵莹严龙飞严文静章建浩南京农业大学食品科学技术学院江苏南京210095 南京苏曼等离子体工程研究院江苏南京210095

草莓采后微生物污染目前已成为物流过程中安全品质控制的研究热点,本研究以菌落总数为响应值,在等离子体活化水(plasma activated water,PAW)制备时间、水杨酸浓度、浸泡时间,介质阻挡放电(dielectric barrier discharge,DBD)低温等离... 详细信息

草莓采后微生物污染目前已成为物流过程中安全品质控制的研究热点,本研究以菌落总数为响应值,在等离子体活化水(plasma activated water,PAW)制备时间、水杨酸浓度、浸泡时间,介质阻挡放电(dielectric barrier discharge,DBD)低温等离子体工作电压、处理时间和工作频率的单因素试验基础上,采用Plackett-Burman试验筛选出对草莓菌落总数影响高度显著的因素,并通过Box-Behnken试验探究PAW及DBD联合处理对草莓冷杀菌效果及感官品质的影响。结果表明:草莓表面菌落总数随PAW制备时间的延长及水杨酸浓度的增大呈先减少后趋于平缓的趋势;随DBD工作电压的增大及处理时间的延长呈逐渐减少的趋势,但超过60 kV或150 s时草莓出现褪色甚至表观破损;浸泡时间及DBD工作频率对菌落总数无显著影响(P>0.05);对草莓菌落总数影响高度显著的因素主次顺序为水杨酸浓度>PAW制备时间>DBD工作电压(P<0.001),且水杨酸浓度和DBD工作电压的临界值均随PAW制备时间延长呈线性下降的趋势;回归优化结果为PAW制备时间96 s、水杨酸浓度1.1 mmol/L、DBD工作电压46 kV,此条件下草莓表面的菌落总数由3.64(lg(CFU/g))降至0.83(lg(CFU/g)),杀菌率达99.8%,在20℃贮藏8 d内明显抑制表面微生物生长,有效维持生鲜品质,延长保鲜期。

关键词：草莓低温等离子体冷杀菌水杨酸菌落总数贮藏期生鲜品质

THE MEMBRANE PROTEINS OF LEAF CELL MEMBRANE OF Vicia faba

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Chinese Science Bulletin 1988年第3期33卷 231-235页

作者：王育东阎隆飞 Laboratory of Biochemistry Beijing Agricultural University

The membranes of animal cells, especially those of human red cells, have been intensively studied. It has been demonstrated that there is a membrane skeleton on the inside of the red cell membrane. The membrane skeleton is composed of membrane proteins such as spectrin, ankyrin and actin. The presence of a similar membrane skeleton in other animal cells was reported also.

关键词： Vicia skeleton CELL Proteins beans bands weights intact reagent stained

Bending Stiffness of Concrete-Filled Steel Tube and Its Influence on Concrete Placement Timing of Composite Beam-String Structure

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Structural Durability & Health Monitoring 2025年第1期19卷 55-75页

作者： Zhenyu Zhang Quan Jin Haitao Zhang Zhao Liu Yuyang Wu longfei Zhang Renzhang yan CCCC Third Highway Engineering Co. Ltd.Beijing101300China School of Civil Engineering Chongqing Jiaotong UniversityChongqing400074China

When the upper chord beam of the beam-string structure(BSS)is made of concrete-filled steel tube(CFST),its overall stiffness will change greatly with the construction of concrete placement,which will have an impact on the design of the tensioning plans and selection of control measures for the *** order to accurately obtain the bending stiffness of CFST beam and clarify its impact on the mechanical properties of composite BSS during construction,the influence of some factors such as height-width ratio,wall thickness of steel tube,elasticity modulus of concrete,and friction coefficient on the bending stiffness are analyzed parametrically by the numerical simulation technology based on an actual *** calculation formula of the equivalent bending stiffness of CFST is also established through mathematical statistical ***,the equivalent bending stiffness is introduced into the construction and use stages of the composite BSS,respectively,and the mechanical properties such as prestress-tensioning control value,structural deformation,and internal force of key members are comparatively analyzed when adopting two different construction ***,the optimal construction plan of concrete placement first and then prestress-tensioning is proposed.

关键词： Concrete panels freeze-thaw damage compression-shear crack tension-shear crack

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论刑罚执行变更的诉讼化改造

人民司法 2020年第19期 68-71页

作者：闫龙飞天津市高级人民法院

刑罚执行变更是罪刑相适应原则在刑罚执行阶段的体现,尤其是减刑和假释制度,对于充分发挥刑罚价值、维护司法权威具有重要意义。但在实践中,由于变更程序缺乏诉讼品质,司法权受到执行机关的不当侵蚀,违法减刑、假释案件频发,严重破坏了... 详细信息

刑罚执行变更是罪刑相适应原则在刑罚执行阶段的体现,尤其是减刑和假释制度,对于充分发挥刑罚价值、维护司法权威具有重要意义。但在实践中,由于变更程序缺乏诉讼品质,司法权受到执行机关的不当侵蚀,违法减刑、假释案件频发,严重破坏了审判权成与司法公信。刑罚执行变更属于典型的司法权,因此有必要对刑罚执行变更程序进行诉讼化改造,赋予被执行人和原公诉机关独立的诉讼地位,引入信用变更制度,由被执行人提出变更请求,检察机关承担反对变更的举证责任,人民法院居中裁判,执行机关只负责教育管理罪犯和配合检察机关取证,彻底消除执行部门对司法权的僭越。同时赋予被执行人和原案被害人充分的律师帮助,确保刑罚执行变更公平公正有效运行。

关键词：罪刑相适应原则检察机关公诉机关被执行人举证责任假释制度诉讼地位司法公信

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玉米胚的肌动蛋白

北京农业大学学报 1987年第3期 273-275页

作者：顾军阎隆飞

近年来、关于非肌细胞中存在类似肌肉细胞的的肌动蛋白的报道陆续发表了很多。肌动蛋白存在于高等植物组织中的报道也不少本文证明在玉米胚组织中存在肌动蛋白。材料和方法玉米胚取自普通玉米(Zea mays)种了。抗粘菌肌动蛋白抗体的制备... 详细信息

近年来、关于非肌细胞中存在类似肌肉细胞的的肌动蛋白的报道陆续发表了很多。肌动蛋白存在于高等植物组织中的报道也不少本文证明在玉米胚组织中存在肌动蛋白。材料和方法玉米胚取自普通玉米(Zea mays)种了。抗粘菌肌动蛋白抗体的制备参见 Lazarides。

关键词：玉米胚离子肌动蛋白肌纤蛋白肌肉蛋白肌动球蛋白肌纤凝蛋白