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Arbuscular mycorrhizal fungi regulate plant mineral nutrient uptake and partitioning in iron ore tailings undergoing eco-engineered pedogenesis

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Pedosphere 2024年第2期34卷 385-398页

作者： Zhen LI Songlin WU Yunjia LIU Qing YI Merinda HALL Narottam SAHA Junjian WANG Yuanfang huang longbin huang College of Land Science and Technology China Agricultural UniversityBeijing 100193China Centre for Mined Land Rehabilitation Sustainable Minerals InstituteThe University of QueenslandBrisbaneQLD 4072Australia Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen 518055China

Excess available K and Fe in Fe ore tailings with organic matter amendment and water-deficiencies may restrain plant colonization and growth,which hinders the formation of eco-engineered soil from these tailings for sustainable and cost-effective mine site *** mycorrhizal(AM)fungi are widely demonstrated to assist plant growth under various unfavorable ***,it is still unclear whether AM symbiosis in tailings amended with different types of plant biomass and under different water conditions could overcome the surplus K and Fe stress for plants in Fe ore tailings,and if so,by what ***,host plants(Sorghum *** ***),either colonized or noncolonized by the AM fungi(Glomus spp.),were cultivated in lucerne hay(LH,C:N ratio of 18)-or sugarcane mulch(SM,C:N ratio of 78)-amended Fe ore tailings under well-watered(55%water-holding capacity(WHC)of tailings)or water-deficient(30%WHC of tailings)*** mycorrhizal colonization,plant growth,and mineral elemental uptake and partitioning were *** indicated that AM fungal colonization improved plant growth in tailings amended with plant biomass under water-deficient *** mycorrhizal fungal colonization enhanced plant mineral element uptake,especially P,both in the LH-and SM-amended tailings regardless of water ***,AM symbiosis development restrained the translocation of excess elements(i.e.,K and Fe)from plant roots to shoots,thereby relieving their *** AM fungal roles in P uptake and excess elemental partitioning were greater in LH-amended tailings than in SM-amended *** deficiency weakened AM fungal colonization and functions in terms of mineral element uptake and *** findings highlighted the vital role AM fungi played in regulating plant growth and nutrition status in Fe ore tailings technosol,providing an important basis for involvement of AM fungi in the eco-engineered pedo

关键词： Fe stress fungal symbiosis K stress mine site rehabilitation mycorrhizal colonization plant biomass amendment plant nutrition water deficiency

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Diversity of As Metabolism Functional Genes in Pb-Zn Mine Tailings

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Pedosphere 2017年第3期27卷 630-637页

作者： LI Xiaofang Philip L.BOND longbin huang Centre for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Centre for Mined Land Rehabilitation Sustainable Minerals Institute The University of Queensland Advanced Water Management Centre The University of Queensland

Microbial activities impact arsenic(As) transformation in mine tailings substantially, yet little is understood on the functional diversity of As metabolism genes. This study explored this issue using a metagenomic approach coupled by a local BLASTN procedure established in our recent studies. An assembled metagenome, recovered from hypersaline and sulfidic mine tailings, was screened for As metabolism genes aio A, arr A, ars C and ars M. This was done using a local BLASTN procedure against databases of the As metabolism genes built in this study. Putative As metabolism genes detected in the assembled metagenome included 11 ars M, 20 ars C and 1 arr A full-length sequences. Together with the rRNA-based phylogenetic profiling results, a picture depicting microbial As cycling in the tailings to the genus level was obtained. It was found that most of the dominant genera in the tailings potentially harboured the genes for As reduction and/or methylation. In particular, a typical pyrite-eater present in the tailings, Thioalkalivibrio sp., was found to harbour not only ars C and ars M, but also arr A. These results highlight the unexpected diversity of As metabolism genes in the tailings, especially considering the extremely low species diversity therein. The microbial As cycling picture established here has potential use for guiding the purposeful manipulation of As biogeochemistry in the tailings.

关键词：物种多样性功能基因铅锌尾矿代谢功能微生物活性系统发育分析生物地球化学砷

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Arbuscular mycorrhiza and plant chromium tolerance

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Soil Ecology Letters 2019年第3期1卷 94-104页

作者： Songlin Wu Xin Zhang longbin huang Baodong Chen State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Environment Centres(CMLR) Sustainable Minerals InstituteThe University of QueenslandBrisbaneQueensland 4072Australia University of Chinese Academy of Sciences Beijing 100049China

Arbuscular mycorrhizal(AM)fungi are ubiquitous soil fungi that form symbiotic associations with most terrestrial *** growth and functions of AM fungi depend on carbohydrates supplied by the plants,in return,the fungi assist the plants to acquire mineral nutrients(e.g.,phosphorus)from *** AM symbiosis also improves plant survival in various unfavorable environments,such as metal(loid)contaminated *** has been well demonstrated that AM symbiosis improved plant adaptation to Cr contamination,which would have a great potential in phytoremediation and ecological restoration of Cr contaminated *** this paper,we have reviewed the role of AM fungi in alleviation of Cr phytotoxicity and associated factors influencing plant Cr *** symbiosis improves plant Cr tolerance through its direct roles in Cr stabilization and transformation and indirect roles via AM symbiosis mediated nutrient acquisition and physiological *** research on physiological and molecular mechanisms underlying Cr behavior and detoxification in AM symbiosis,as well as potential use of AM fungi in ecological restoration and agriculture production in Cr contaminated soils were also proposed.

关键词： Arbuscular mycorrhizal fungi Heavy metal Chromium Tolerance Translocation and transformation Bioremediation

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一种基于差分隐私的边缘计算隐私数据保护算法

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科学技术创新 2023年第1期 134-139页

作者：黄龙彬李峰武汉航城智慧科技有限公司湖北武汉

随着计算技术与物联网的快速发展,人们能够收集与共享海量数据,其中物联网的传感器更是收集大量数据的入口。同时,人们在处理由于海量设备接入产生的海量数据方面面临着艰巨的挑战,边缘计算开始受到大众的关注。虽然海量数据为信息发现... 详细信息

随着计算技术与物联网的快速发展,人们能够收集与共享海量数据,其中物联网的传感器更是收集大量数据的入口。同时,人们在处理由于海量设备接入产生的海量数据方面面临着艰巨的挑战,边缘计算开始受到大众的关注。虽然海量数据为信息发现提供了巨大的机会,但隐私泄露的问题也层出不穷。当数据发布者发布各种统计信息时,攻击者可以无需联系用户或数据发布者而只通过简单的查询功能获取数据中的统计规律,因此,如何保护统计信息的数据隐私成为人们关注的焦点,提出了一种基于抽样分组的差分隐私直方图发布方法,此方法能够在满足差分隐私保护的同时,有效提升数据的可用性。

关键词：边缘计算差分隐私直方图发布

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Iron Fortification in Parboiled Rice—A Rapid and Effective Tool for Delivering Iron Nutrition to Rice Consumers

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Food and Nutrition Sciences 2011年第4期2卷 323-328页

作者： Chanakan Prom-u-thai longbin huang Shu Fukai Benjavan Rerkasem 不详

Parboiled rice production accounts for nearly half of the world’s rice production. Its markets and consumer base are firmly established in South Asia and Africa where Fe-deficient populations are mostly concentrated. Our research group has pioneered the technology of Fe-fortification in parboiled rice and demonstrated its feasibility in significantly increasing Fe concentration in the endosperm (white rice) and its bioavailability in rice based diet. Fortification with Fe-EDTA during parboiling resulted in 10 to 50 folds increase in grain Fe concentration, depending on the grain properties among different rice varieties. However, the broken rice of Fe-fortified parboiled rice contained 5 times the Fe concentration of the full grain, which is often bought and consumed by people in low income category. The bioavailability of the fortified Fe is closely correlated with increasing Fe concentration in white rice (r = 0.90, p 50% to almost 100%, despite repeated rinsing before cooking depending on rice varieties. Perls’ Prussian blue staining and prolonged polishing showed that the in vitro Fe penetrated into the interior of the endosperm. Fortification at the rate up to 250 mg Fe kg–1 paddy rice has no deleterious effects on appearance, color and sensory quality and overall acceptance by parboiled rice consumers. It increased Fe concentration up to 27 mg Fe kg–1 of in white rice, compared with 5 mg Fe kg–1 in unfortified parboiled and raw white rice. As a result, we can conclude that parboiled rice is a ready and effective tool for improving Fe nutrition of rice consumers in these regions.

关键词： Fe Deficiency Anemia Fe Fortified Parboiled Rice Fe Bioavailability

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Response of microbial communities to biochar-amended soils:a critical review

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Biochar 2019年第1期1卷 3-22页

作者： Kumuduni Niroshika Palansooriya James Tsz Fung Wong Yohey Hashimoto longbin huang Jörg Rinklebe Scott X.Chang Nanthi Bolan Hailong Wang Yong Sik Ok Korea Biochar Research Center O-Jeong Eco-Resilience Institute(OJERI)Division of Environmental Science and Ecological EngineeringKorea UniversitySeoul 02841Republic of Korea Department of Civil and Environmental Engineering The Hong Kong University of Science and TechnologyHong Kong SARChina Department of Bioapplications and Systems Engineering Tokyo University of Agriculture and TechnologyTokyoJapan Environment Centres Sustainable Minerals InstituteThe University of QueenslandBrisbaneQLD 4072Australia Laboratory of Soil-and Groundwater-Management School of Architecture and Civil EngineeringInstitute of Foundation Engineering and Water and-Waste ManagementUniversity of WuppertalPauluskirchstraße 742285 WuppertalGermany Department of Environment Energy and GeoinformaticsSejong UniversitySeoul 05006Republic of Korea Department of Renewable Resources University of AlbertaEdmontonAB T6G 2E3Canada Global Centre for Environmental Remediation University of NewcastleCallaghanNSW 2308Australia School of Environmental and Chemical Engineering Foshan UniversityFoshan 528000GuangdongChina Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province Zhejiang A&F University

Application of biochar to soils changes soil physicochemical properties and stimulates the activities of soil microorganisms that influence soil quality and plant *** the response of soil microbial communities to biochar amendments is important for better understanding interactions of biochar with soil,as well as ***,the effect of biochar on soil microorganisms has received less attention than its influences on soil physicochemical *** this review,the following key questions are discussed:(i)how does biochar affect soil microbial activities,in particular soil carbon(C)mineralization,nutrient cycling,and enzyme activities?(ii)how do microorganisms respond to biochar amendment in contaminated soils?and(iii)what is the role of biochar as a growth promoter for soil microorganisms?Many studies have demonstrated that biochar-soil application enhances the soil microbial biomass with substantial changes in microbial community *** amendment changes microbial habitats,directly or indirectly affects microbial metabolic activities,and modifies the soil microbial community in terms of their diversity and ***,chemical properties of biochar,(especially pH and nutrient content),and physical properties such as pore size,pore volume,and specific surface area play significant roles in determining the efficacy of biochar on microbial performance as biochar provides suitable habitats for *** mode of action of biochar leading to stimulation of microbial activities is complex and is influenced by the nature of biochar as well as soil conditions.

关键词： Carbon cycling Microbial habitat Microorganisms Nutrient cycling Enzyme activity

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燕麦蛋白耦合异源共架技术对大米蛋白水溶性的影响

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食品工业科技 2024年第7期45卷 134-141页

作者：黄馨禾王长艳范龙彬常雅宁华东理工大学食品科学与工程系生物反应器工程国家重点实验室上海200237 上海徐汇区地下空间开发有限公司上海200030

为了通过燕麦蛋白耦合异源共架技术提升大米蛋白在水中的溶解特性,本研究以不同比例的大米蛋白和燕麦蛋白为原料,将两种蛋白在pH12的环境下混合,再恢复至中性进行共架。测定蛋白的溶解性并通过分析十二烷基磺酸钠-聚丙烯酰胺凝胶电泳、... 详细信息

为了通过燕麦蛋白耦合异源共架技术提升大米蛋白在水中的溶解特性,本研究以不同比例的大米蛋白和燕麦蛋白为原料,将两种蛋白在pH12的环境下混合,再恢复至中性进行共架。测定蛋白的溶解性并通过分析十二烷基磺酸钠-聚丙烯酰胺凝胶电泳、粒径、微观形态、乳化特性、起泡特性、傅里叶红外光谱等探究其作用机理。结果显示,大米-燕麦蛋白的溶解度显著高于大米蛋白(8.49%±1.53%)(P<0.05),两者比例为1:0.6时达到最大值93.07%±2.15%。经过异源共架处理的大米-燕麦蛋白复合物粒径为0.1μm左右,同时结构更加松散,大米蛋白和燕麦蛋白之间的疏水相互作用和氢键也发生了变化,使得大米蛋白的溶解度增加,进一步提升乳化特性和起泡特性。综上,燕麦蛋白耦合异源共架技术是一种可以解决大米蛋白水溶性差的可靠手段。

关键词：大米蛋白燕麦蛋白功能特性异源共架蛋白结构

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基于YOLOv8的铁路隧道渗漏水智能检测

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交通科技 2024年第3期 110-114,125页

作者：邓琳朱杰黄超闫龙宾中国水利水电第八工程局有限公司长沙410004 中南大学土木工程学院长沙410075

铁路隧道渗漏水会影响隧道的结构稳定性和运营安全,渗漏水病害的自动化检测亟待解决。传统的人工巡检方法自动化程度较低,检测效率低下,容易出现错检漏检,无法满足大规模隧道的快速检测需求。针对这一问题,提出了一种基于YOLOv8网络的... 详细信息

铁路隧道渗漏水会影响隧道的结构稳定性和运营安全,渗漏水病害的自动化检测亟待解决。传统的人工巡检方法自动化程度较低,检测效率低下,容易出现错检漏检,无法满足大规模隧道的快速检测需求。针对这一问题,提出了一种基于YOLOv8网络的铁路隧道渗漏水智能检测方法,并在自建的铁路隧道渗漏水数据集中进行模型训练和参数调优。实验结果表明,在不同版本的模型实验中,YOLOv8-n网络的综合性能最好。在不同模型对比实验中,YOLOv8模型的F_(1)分数、AP值分别为81.28%,81.38%,相比于YOLOv7、YOLOv5、SSD模型。分别提高了6.85%,6.89%,9.40%、8.19%,12.19%、10.57%。综合分析可得,YOLOv8模型综合性能最优秀,适用于铁路隧道工程的渗漏水检测任务。

关键词：铁路隧道隧道渗漏水深度学习目标检测

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薯蓣皂苷对脓毒症大鼠肾损伤的影响及机制

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中国药房 2024年第11期35卷 1334-1338页

作者：申翔徐尚刚黄洋辉罗斌周裕丰梁隆斌成都大学附属医院急诊科成都610081

目的探究薯蓣皂苷对脓毒症大鼠肾损伤的影响及可能机制。方法采用盲肠结扎和穿刺法建立脓毒症大鼠模型。将建模成功的60只大鼠按照随机数字表法分为模型组(0.5%羧甲基纤维素钠溶液),薯蓣皂苷低、中、高剂量组(30、60、120 mg/kg)和地... 详细信息

目的探究薯蓣皂苷对脓毒症大鼠肾损伤的影响及可能机制。方法采用盲肠结扎和穿刺法建立脓毒症大鼠模型。将建模成功的60只大鼠按照随机数字表法分为模型组(0.5%羧甲基纤维素钠溶液),薯蓣皂苷低、中、高剂量组(30、60、120 mg/kg)和地塞米松组(阳性对照,10 mg/kg),每组12只;另取12只大鼠作为假手术组(0.5%羧甲基纤维素钠溶液)。造模15 min后,各组大鼠尾静脉注射给药/0.5%羧甲基纤维素钠溶液。给药24 h后,检测大鼠血清中肌酐(Cr)、尿素氮(BUN)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、肾损伤分子1(KIM-1)、白细胞介素6(IL-6)、IL-1β、肿瘤坏死因子α(TNF-α)水平以及肾组织中丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性和核因子E2相关因子2(Nrf2)、血红素加氧酶1(HO-1)、NOD样受体蛋白3(NLRP3)表达量,并观察肾组织病理形态学变化。结果与模型组比较,薯蓣皂苷低、中、高剂量组大鼠肾组织病理损伤明显改善,血清中Cr、BUN、NGAL、KIM-1、IL-6、IL-1β、TNF-α水平和肾组织中MDA水平、NLRP3蛋白表达量均显著降低(P0.05)。结论薯蓣皂苷可能是通过激活Nrf2/HO-1信号通路抑制NLRP3炎性小体,实现对炎症因子表达和氧化应激的抑制,从而发挥对脓毒症肾损伤的保护作用。

关键词：薯蓣皂苷 Nrf2/HO-1信号通路 NLRP3炎性小体脓毒症肾损伤

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实系数多项式实根范围的探讨

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高等数学研究 2024年第4期27卷 27-28页

作者：黄香蕉明万元赵龙斌南昌航空大学数学与信息科学学院江西南昌330063 河南理工大学数学与信息科学学院河南焦作454000

针对实系数多项式方程,在已知一个实根的条件下,给出了其余实根上、下界的估计.然后将结论推广至已知多个实根的情形,并通过例题加以说明.

关键词：多项式实根上界

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