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单细胞分选与测序技术在环境微生物领域的应用及前景

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南京农业大学学报 2024年第3期47卷 415-423页

作者：郭晗玥王礼岳阮杨徐琪程凌宁沈其荣南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化高新技术研究重点实验室江苏南京210095

随着对环境微生物生态系统认知的深化,对其深入研究的需求持续增加,传统的培养组学和环境DNA测序等研究手段已经难以满足对微生物物种和功能多样性的全面了解。单细胞分选与测序技术为环境微生物研究开创了全新的视角,成为该领域的热点... 详细信息

随着对环境微生物生态系统认知的深化,对其深入研究的需求持续增加,传统的培养组学和环境DNA测序等研究手段已经难以满足对微生物物种和功能多样性的全面了解。单细胞分选与测序技术为环境微生物研究开创了全新的视角,成为该领域的热点与前沿。单细胞技术将微生物研究的焦点从群落水平深入到个体细胞层面,通过精准分选并测序单个微生物细胞,深入挖掘微生物的种间和种内多样性以及功能特性。本综述介绍单细胞技术在环境微生物研究中的应用,详细论述单细胞分选和基因组扩增的关键过程,并探讨单细胞测序数据解析过程中的生物信息学基本流程,为单细胞分选与测序技术在环境微生物研究中的发展提供借鉴和启示。

关键词：单细胞分选单细胞测序单细胞基因组环境微生物

A boundary surrogate model for micro/nano grooved surface structure applied in turbulence flow control over airfoil

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Chinese Journal of Aeronautics 2022年第2期35卷 62-73页

作者： liyue wang Cong wang Shuyue wang Sheng QIN Gang SUN Bo YOU Yongjian ZHONG Department of Aeronautics&Astronautics Fudan UniversityShanghai 200433China Department of Material Science Fudan UniversityShanghai 200433China AECC Commercial Aircraft Engine Co. Ltd.Shanghai 200241China

The application of grooved surface structure is an emerging and effective means in turbulence flow ***,for a realistic configuration,the global flow field described directly by simple application of massive grids makes it unfeasible to *** this paper,a boundary surrogate model reproducing the effect of microscopic near-wall region is proposed to improve computational *** surrogate model trained with Lattice Boltzmann Method(LBM)considering the rarefied effect based on real micro/nanoflow field is new among literature,which accurately shows flow characteristics of the micro/nano *** this approach,numerical simulations via Reynolds-averaged Navier Stokes equations with modified wall boundary condition are performed in subsonic and transonic *** results show that micro/nano grooved surface structure has the effect of delaying transition from laminar to turbulence,thus reducing the skin friction *** of turbulence intensity and turbulence kinetic energy shows that the near-wall flow field of grooved airfoil is more stable compared with that of the smooth *** reducing rate of maximum turbulent intensity reaches 13.39%.The paper shows a perspective for further application of micro/nano groove structure to turbulence flow control in aircraft design by providing an accurate and efficient simulation method.

关键词： Boundary modeling CFD Drag reduction Flow control Microstructure

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基于集成学习的电商销量预测研究分析

长春大学学报 2024年第4期34卷 1-7页

作者：张晓颖贺伊雯王立越长春大学理学院长春130022

针对电商销量数据的复杂性和现有预测模型的稳定性及泛化能力不足问题,本研究基于大样本多变量数据,应用随机森林和渐进梯度回归树等机器学习模型进行分析。研究结果表明,相比于广义线性回归、弹性网络、支持向量回归、KNN回归树、决策... 详细信息

针对电商销量数据的复杂性和现有预测模型的稳定性及泛化能力不足问题,本研究基于大样本多变量数据,应用随机森林和渐进梯度回归树等机器学习模型进行分析。研究结果表明,相比于广义线性回归、弹性网络、支持向量回归、KNN回归树、决策树、多层感知机、AdaBoost,随机森林和渐进梯度回归树对电商销售数据预测拟合更加精确。相比于广义线性回归、弹性网络等7种传统机器学习算法,随机森林和渐进梯度回归树这两种集成学习的方法对电商销量预测更加精确,且渐进梯度回归树算法拟合效果更好、均方根误差更小,是一种更加有效的电商销量预测方法。

关键词：随机森林 GBRT 电商销量机器学习

毒害艾美耳球虫谷胱甘肽过氧化物酶EnGPX的原核表达与分析

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畜牧兽医学报 2024年第2期55卷 846-853页

作者：彭月梅叶状汪飞燕王礼跃冯永翠王乐乐候照峰许金俊陶建平刘丹丹扬州大学兽医学院扬州225009 扬州大学江苏高校动物重要疫病与人兽共患病防控协同创新中心扬州225009

旨在研究毒害艾美耳球虫谷胱甘肽过氧化物酶EnGPX的反应原性及其在虫体内的亚细胞定位。提取毒害艾美耳球虫(扬州株)配子体总RNA,RT-PCR扩增En GPX的ORF编码序列,构建原核表达质粒pET-28a(+)-En GPX,转化至BL21(DE3)进行体外诱导表达,... 详细信息

旨在研究毒害艾美耳球虫谷胱甘肽过氧化物酶EnGPX的反应原性及其在虫体内的亚细胞定位。提取毒害艾美耳球虫(扬州株)配子体总RNA,RT-PCR扩增En GPX的ORF编码序列,构建原核表达质粒pET-28a(+)-En GPX,转化至BL21(DE3)进行体外诱导表达,同时制备鼠抗rEnGPX多克隆抗体,对重组蛋白进行Western blot反应原性分析和激光共聚焦免疫荧光定位分析。结果表明,En GPX ORF序列全长753 bp,编码250个氨基酸,体外重组表达蛋白大小约30 ku,主要以包涵体形式存在。该重组蛋白能被6×HIS标签单克隆抗体,鼠抗rEnGPX多克隆抗体,毒害艾美耳球虫、巨型艾美耳球虫和柔嫩艾美耳球虫病鸡康复血清所识别,表明其具有较好的反应原性和交叉反应原性。在天然配子体蛋白中检测出EnGPX,其编码蛋白主要分布于配子体内的Ⅱ型成壁体(WFBII)及卵囊壁上。本研究成功克隆表达了毒害艾美耳球虫谷胱甘肽过氧化物酶EnGPX,验证其具有良好的反应原性,并定位于配子体及卵囊壁上。以期为研究En GPX参与卵囊壁形成的分子机制提供新的线索,也为研制新型球虫亚单位疫苗提供新的靶标。

关键词：毒害艾美耳球虫 EnGPX 克隆表达反应原性免疫荧光定位

Discussion on the Effectiveness of Elevating HDL-C in Treating Cardiovascular Diseases of Patients with Type 2 Diabetes Mellitus

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International Journal of Clinical Medicine 2019年第4期10卷 293-305页

作者： Shasha wang liyue wang Medical College Wuhan University of Science and Technology Wuhan China Puren Hospital Affiliated to Wuhan University of Science and Technology Internal Medicine-Cardiovascular Department Wuhan China

High density lipoprotein protects cardiovascular diseases and reduces the risk of cardiovascular diseases through cholesterol reverse transport and other mechanisms. High-density lipoprotein cholesterol (HDL-C) is an independent predictor of negative events in cardiovascular diseases. Low concentration of HDL-C indicates abnormal regulation of HDL anabolism. Various proteins and receptors such as cholesteryl ester transfer protein (CETP) are involved in HDL anabolism. Type 2 Diabetes Mullitu and its related metabolic syndrome, chronic inflammation as well as oxidative stress not only affect the proteins and receptors related to HDL anabolism, but also affect their functional changes, making HDL change from anti-inflammatory, antioxidant, protecting endothelial cell function to pro-inflammatory, pro-oxidative and pro-endothelial cell apoptosis. This article will describe the relationship between HDL-C, type 2 diabetes and cardiovascular diseases from the effects of T2DM on HDL anabolism and function, and further explore the effectiveness of elevating HDL-C in treating cardiovascular diseases of patients with type 2 diabetes.

关键词： HDL Type 2 Diabetes Mellitus Cardiovascular Diseases Reverse Cholesterol Transport

鸡球虫重组蛋白rEnApiAP2对鸡免疫保护效果的观察

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畜牧兽医学报 2024年第4期55卷 1716-1727页

作者：王乐乐王礼跃蔡为民康喜龙冯茜茜张知之范雪莲朱玉刘丹丹许金俊潘志明陶建平扬州大学兽医学院扬州225009 江苏动物重要疫病与人兽共患病防控协同创新中心扬州225009 江苏省人兽共患病学重点实验室扬州225009 宿迁学院宿迁223800

将毒害艾美耳球虫重组蛋白rEnApiAP2单独免疫(单免)或分别与柔嫩艾美耳球虫重组蛋白rEtGAM56或rEtGAM59联合免疫(联免)雏鸡,同时设rEtGAM56单免、rEtGAM59单免、未免疫攻虫和空白组为对照,分别攻毒害艾美耳球虫或柔嫩艾美耳球虫,以成活... 详细信息

将毒害艾美耳球虫重组蛋白rEnApiAP2单独免疫(单免)或分别与柔嫩艾美耳球虫重组蛋白rEtGAM56或rEtGAM59联合免疫(联免)雏鸡,同时设rEtGAM56单免、rEtGAM59单免、未免疫攻虫和空白组为对照,分别攻毒害艾美耳球虫或柔嫩艾美耳球虫,以成活率、排出血便数、平均增重、相对增重率、病变记分、卵囊减少率、抗球虫指数(ACI)和血清抗体水平为指标,评价rEnApiAP2单免及与rEtGAM联免的免疫保护效果。结果显示:各试验组的成活率均为100%;与未免疫攻虫组比较,各免疫组鸡排血便堆数减少、平均增重增加。在rEnApiAP2单免组间,低剂量组的相对增重率(82.36%)、卵囊减少率(73.88%)和抗球虫指数(155.26)均为最高,平均病变记分最低(1.71)。攻毒害艾美耳球虫后,联免组的ACI值均大于相应的单免组,其中rEnApiAP2与rEtGAM56联免组的ACI值(169.83)最高。攻柔嫩艾美耳球虫后,rEnApiAP2单免组的ACI值(128.37)低于rEtGAM56、rEtGAM59单免组(130.12、151.88),rEnApiAP2与rEtGAM59联免组的ACI值(141.62)接近于rEtGAM59单免组,rEnApiAP2与rEtGAM56联免组的ACI值(115.46)小于rEtGAM56单免组。各免疫组血清抗体水平均显著高于空白对照组(P<0.05)。rEnApiAP2对毒害艾美耳球虫具有一定的免疫保护效果,与rEtGAM联免可提高对毒害艾美耳球虫的免疫保护力。本研究结果为鸡球虫病重组亚单位疫苗研制奠定了基础。

关键词：毒害艾美耳球虫柔嫩艾美耳球虫 rEnApiAP2 rEtGAM 保护效果

A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

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Chinese Chemical Letters 2020年第1期31卷 125-128页

作者： Yangyang Yang Chen Zhang Rizhao Pan Shiwei Zhang Shengtao Yao Yao Tang Weilong Zhu liyue wang Weiping Zhu Yufang Xu Xuhong Qian Shanghai Key Laboratory of Chemical Biology School of PharmacyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237China

Alkaline phosphatase(ALP)is one of essential biomarkers in mammalian *** we report a ratiometric probe for ALP,which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide(BTHPB).The enzymatic dephosphorylation converts the probe to BTHPB,which exhibits a large spectral red-shift(120 nm),allowing extremely high sensitivity of ALP sensing at 0.004 mU/*** probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.

关键词： Fluorescent probe Alkaline phosphatase Ratiometric ESIPT Biomarker

河北省邯郸市畜禽生产用品销售情况调查与分析

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养殖与饲料 2024年第6期23卷 37-40页

作者：烟玉华刘国芹王利月王彦勇王日田赵海燕陈丹丹郭琛琛邯郸科技职业学院农牧系河北邯郸056046 河北省磁县白土镇农业综合服务中心河北磁县056500 河北省辛集市南智邱动物卫生监督分所河北辛集050000

[目的]分析河北省邯郸市畜牧业的发展趋势,了解行业生产力水平、规模化程度、消费理念变化及安全生产需求。[方法]以邯郸地区有代表性的畜禽生产用品销售公司为调查对象,运用实地观察、访谈调查、网络询问、问卷调查等方法,统计2020—2... 详细信息

[目的]分析河北省邯郸市畜牧业的发展趋势,了解行业生产力水平、规模化程度、消费理念变化及安全生产需求。[方法]以邯郸地区有代表性的畜禽生产用品销售公司为调查对象,运用实地观察、访谈调查、网络询问、问卷调查等方法,统计2020—2022年的销售端数据。[结果]畜牧业从业人员中青年比例上升,2022年较2020年增长约50%;大专、本科及以上学历者占比逐年增加,2022年大专生占比近40%;预防用药意识从2020年的30%上升到2022年的70%左右;2022年规模化养殖数量占比较2020年增加30%左右;泔水使用率逐年下降,从2018年的7%下降到2022年不足1%;年销售额先增多后减少,与2022年新冠肺炎疫情有关;销售途径以实体店与电商销售为主;预防保健型的中药产品,销量增加约45%左右;治疗用药占比减少,尤其是抗生素产品下降约40%左右。[结论]近三年现代畜牧业发展呈现出从业人员年龄年轻化、生产走向规模化、商品消费理性化、养殖生产安全化的发展趋势。

关键词：畜禽业发展兽药饲料销售端数据产业化调查方法

Genome-wide identification of the CONSTANS-LIKE(COL)family and mechanism of fruit senescence regulation by PpCOL8 in sand pear(Pyrus pyrifolia)

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Journal of Integrative Agriculture 2024年第4期23卷 1222-1237页

作者： Yue Xu Shurui Song Huiying wang Xilong Cao Xinran Zhao Wenli wang liyue Huo Yawei Li Misganaw Wassie Bin Lu Liang Chen Haiyan Shi College of Horticulture Hebei Agricultural UniversityBaoding 071001China Wuhan Botanical Garden Chinese Academy of SciencesWuhan 430074China

Pyrus pyrifolia Nakai‘Whangkeumbae'is a sand pear fruit with excellent nutritional quality and ***,the industrial development of pear fruit is significantly limited by its short shelf *** acid(SA),a well-known phytohormone,can delay fruit senescence and improve shelf ***,the mechanism by which SA regulates CONSTANS-LIKE genes(COLs)during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly *** this study,22 COL genes were identified in sand pear,including four COLs(Pp COL8,Pp COL9a,Pp COL9b,and Pp COL14)identified via transcriptome analysis and 18 COLs through genome-wide *** COL genes were divided into three subgroups according to the structural domains of the COL *** COL8,with two B-box motifs and one CCT domain,belonged to the first *** contrast,the other three Pp COLs,Pp COL9a,Pp COL9b,and Pp COL14,with similar conserved protein domains and gene structures,were assigned to the third *** four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit ***,the expression of Pp COL8 was inhibited by exogenous SA treatment,while SA up-regulated the expression of Pp COL9a and Pp ***,Pp COL8 interacts with Pp MADS,a MADS-box protein preferentially expressed in fruit,and SA up-regulated its *** the production of ethylene and the content of malondialdehyde(MDA)were increased in Pp COL8-overexpression sand pear fruit,the antioxidant enzyme(POD and SOD)activity and the expression of Pp POD1 and Pp SOD1 in the sand pear fruits were down-regulated,which showed that Pp COL8 promoted sand pear fruit *** contrast,the corresponding changes were the opposite in Pp MADS-overexpression sand pear fruits,suggesting that Pp MADS delayed sand pear fruit *** co-transformation of Pp COL8 and Pp MADS also delayed sand pear fruit *** re

关键词： Pyrus pyrifolia CONSTANS-LIKE gene salicylic acid fruit senescence MADS