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High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

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Neural Regeneration Research 2024年第8期19卷 1772-1780页

作者： Jing Luo Yuan Feng Zhongqiu Hong Mingyu Yin Haiqing Zheng liying zhang Xiquan Hu Department of Rehabilitation Medicine The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Department of Hepatobiliary Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Prolife ration of neural stem cells is crucial for promoting neuronal regeneration and repairing cerebral infarction damage.Transcranial magnetic stimulation(TMS)has recently emerged as a tool for inducing endogenous neural stem cell regeneration,but its underlying mechanisms remain unclea r In this study,we found that repetitive TMS effectively promotes the proliferation of oxygen-glucose deprived neural stem cells.Additionally,repetitive TMS reduced the volume of cerebral infa rction in a rat model of ischemic stro ke caused by middle cerebral artery occlusion,im p roved rat cognitive function,and promoted the proliferation of neural stem cells in the ischemic penumbra.RNA-sequencing found that repetitive TMS activated the Wnt signaling pathway in the ischemic penumbra of rats with cerebral ischemia.Furthermore,PCR analysis revealed that repetitive TMS promoted AKT phosphorylation,leading to an increase in mRNA levels of cell cycle-related proteins such as Cdk2 and Cdk4.This effect was also associated with activation of the glycogen synthase kinase 3β/β-catenin signaling pathway,which ultimately promotes the prolife ration of neural stem cells.Subsequently,we validated the effect of repetitive TMS on AKT phosphorylation.We found that repetitive TMS promoted Ca2+influx into neural stem cells by activating the P2 calcium channel/calmodulin pathway,thereby promoting AKT phosphorylation and activating the glycogen synthase kinase 3β/β-catenin pathway.These findings indicate that repetitive TMS can promote the proliferation of endogenous neural stem cells through a Ca2+influx-dependent phosphorylated AKT/glycogen synthase kinase 3β/β-catenin signaling pathway.This study has produced pioneering res ults on the intrinsic mechanism of repetitive TMS to promote neural function recove ry after ischemic stro ke.These results provide a stro ng scientific foundation for the clinical application of repetitive TMS.Moreover,repetitive TMS treatment may not only be an efficient and potential approach to support neurogenesis for

关键词： AKT/β-catenin signaling brain stimulation Ca2+influx cell proliferation ischemic stroke middle cerebral artery occlusion neural stem cells neurological rehabilitation repetitive transcranial magnetic stimulation

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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

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The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang liying zhang Lihua Deng Zhiguo zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

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Gene expression profiles in early leaf of rice(Oryza sativa)and foxtail millet(Setaria italica)

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The Crop Journal 2024年第3期12卷 776-787页

作者： Jing Sun Chen Deng Xiuru Dai Haoshu Li liying zhang Jingke Wang Hang Zhao Yirong Yang NghiVan Phung Zhiguo zhang Pinghua Li Xuehui Sun Tiegang Lu Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China College of Agronomy Shandong Agricultural UniversityTai’an 271018ShandongChina Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101ShandongChina

Plant anatomy is patterned early during leaf development which suggests studying the spatial–temporal transcriptomes of primordia will help identify critical regulative and functional genes.We successfully isolated the leaf primordia tissues from the C3grass rice and the C4grass foxtail millet by laser capture microdissection(LCM)and studied the gene expression throughout leaf developmental stages.Our data analysis uncovered the conserved expression patterns of certain gene clusters both in rice and foxtail millet during leaf development.We revealed genes and transcription factors involved in vein formation,stomatal development,and suberin accumulation.We identified 79 candidate genes associated with functional regulation of C4anatomy formation.Screening phenotype of the candidate genes revealed that knock-out of a putative polar auxin transport related gene NAL1 resulted significantly reduced veinal space in rice leaf.Our present work provides a foundation for future analyses of genes with novel functions in grasses and their role in leaf development,in particular the role in leaves with a contrasting C3vs.C4biosynthetic pathway.

关键词： Rice Foxtail millet Leaf development RNA-seq LCM

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Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster

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Journal of Environmental Sciences 2023年第3期125卷 616-629页

作者： Zhidi Wang liying zhang Xing Wang College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193China Beijing Key Laboratory of Biodiversity and Organic Farming Beijing 100193China

The widely use of silver nanoparticles(AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in animals.Nevertheless, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila melanogaster.We show here that AgNPs significantly decreased Drosophila fecundity, the third-instar larvae weight and rates of pupation and eclosion in a dose-dependent manner. AgNPs reduced fat body cell viability in MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)assays. AgNPs caused DNA damage in hemocytes and S2 cells. Interestingly, the mRNA levels of the entire metallothionein gene family were increased under AgNPs exposure as determined by RNA-seq analysis and validated by qRT-PCR, indicating that Drosophila responded to the metal toxicity of AgNPs by producing metallothioneins for detoxification. These findings provide a better understanding of the mechanisms of AgNPs toxicity and may provide clues to effect on other organisms, including humans.

关键词： Drosophila melanogaster Silver nanoparticles(AgNPs) Reactive oxygen species(ROS) DNA damage RNA-seq Metallothionein

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Longitudinal Relationship between Gratitude and Benign/Malicious Envy: Evidence from a Cross-Lagged Analysis

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International Journal of Mental Health Promotion 2022年第2期24卷 277-286页

作者： liying zhang Lijun Yang School of Educational Sciences Hunan Normal UniversityChangsha410081China

Though prior research has identiﬁed that gratitude is associated with benign/malicious envy(BeMaS).The pur-pose of this study was to explore the causal relationship between gratitude and BeMaS among Chinese adoles-cents.The two-wave study,in which 906 adolescents participated,includes measurements of gratitude and BeMaS.We employed the structural equation models to test the cross-lagged effect between trait gratitude and BeMaS.The results showed that gratitude could positively predict benign envy and could negatively predict mal-icious envy.Besides,there was no evidence for the reverse or reciprocal relationships between gratitude and BeMaS.Theﬁndings provide further evidence about the causal relationship between gratitude and BeMaS among adolescents.Moreover,these results have implications for gratitude interventions that promote the constructive meaning of envy and reduce the negative inﬂuence of envy.

关键词： Cross-lagged analysis gratitude benign envy malicious envy

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Sdr4 dominates pre-harvest sprouting and facilitates adaptation to local climatic condition in Asian cultivated rice

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Journal of Integrative Plant Biology 2022年第6期64卷 1246-1263页

作者： Bo Zhao Hui zhang Tianxiao Chen Ling Ding liying zhang Xiali Ding Jun zhang Qian Qian Yong Xiang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding College of Life SciencesShanxi Agricultural UniversityTaigu 030801China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China

Pre-harvest sprouting(PHS),which reduces grain yield and quality,is controlled by seed dormancy genes.Because few dormancy-related genes have been cloned,the genetic basis of seed dormancy in rice(Oryza sativa L.)remains unclear.Here,we performed a genome-wide association study and linkage mapping to dissect the genetic basis of seed dormancy in rice.Our findings suggest that Seed Dormancy4(Sdr4),a central modulator of seed dormancy,integrates the abscisic acid and gibberellic acid signaling pathways at the transcriptional level.Haplotype analysis revealed that three Sdr4 alleles in rice cultivars already existed in ancestral Oryza rufipogon accessions.Furthermore,like the semi-dwarf 1(SD1)and Rc loci,Sdr4 underwent selection during the domestication and improvement of Asian cultivated rice.The distribution frequency of the Sdr4-n allele in different locations in Asia is negatively associated with local annual temperature and precipitation.Finally,we developed functional molecular markers for Sdr4,SD1,and Rc for use in molecular breeding.Our results provide clues about the molecular basis of Sdr4-regulated seed dormancy.Moreover,these findings provide guidance for utilizing the favorable alleles of Sdr4 and Rc to synergistically boost PHS resistance,yield,and quality in modern rice varieties.

关键词： climatic adaptation pre-harvest sprouting Rc rice SD1 Sdr4 seed dormancy

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Fe_(3)O_(4)@C 3D foam for strong low-frequency microwave absorption

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Journal of Materiomics 2023年第1期9卷 148-156页

作者： Li Wang zhang Chen Xue Wang liying zhang Zongtao zhang Qi Zhao Yanfeng Gao School of Materials Science and Engineering Shanghai UniversityShanghai200444China Shanghai Collaborative Innovation Center for High Performance Fiber Composites Center for Civil Aviation CompositesDonghua UniversityShanghai201620China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou450066China

Low-frequency microwave absorbing materials have been challenging for many years.Three-dimensional dielectric/magnetic porous materials are beneficial for improving the low-frequency microwave absorbing performance because of natural resonance and improved impedance matching.In this study,Fe_(3)O_(4)@C 3D foam was prepared by carbothermal reduction method and the microwave attenuation performances and mechanisms were studied.By adjusting the content of Fe_(3)O_(4)@C 3D foam in paraffin composites,the low-frequency microwave attenuation capacity could be effectively optimized.The minimum reflection loss(RLmin)of paraffin composite with 40%(in mass fraction)loading exhibits-54.7 dB at 4.1 GHz for a thickness of 4.0 mm.Surprisingly,the paraffin composite with 50%(in mass fraction)loading could almost cover 2–4 GHz(S-band)in the thickness range of 3.5–5.5 mm.The strong low-frequency microwave attenuation property of Fe_(3)O_(4)@C 3D foam is mainly attributed to excellent low-frequency impedance matching,natural resonance,interfacial/dipole polarization,multiple reflection and scattering.This method provides a new perspective for preparing lightweight and high performance low-frequency microwave absorbing materials.

关键词： Fe_(3)O_(4)@C 3D foam Low-frequency microwave absorption

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What should we do about monkeypox?

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Animal Models and Experimental Medicine 2022年第5期5卷 482-484页

作者： Si Chen Xue Li liying zhang Linzhu Ren College of Animal Sciences Key Lab for Zoonoses ResearchMinistry of EducationJilin UniversityChangchunChina

Over the past 3 years,the COVID-19 pandemic has posed a significant global challenge to the public health system.However,other diseases,such as hepatitis of unknown origin in children and monkeypox,have been recently reported in European,African,and American countries.These data showed that monkeypox and other emerging or re-emerging viruses are novel global threats.

关键词： diseases system monkey

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Enhancing Breast Cancer Diagnosis with Channel-Wise Attention Mechanisms in Deep Learning

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Computers, Materials & Continua 2023年第12期77卷 2699-2714页

作者： Muhammad Mumtaz Ali Faiqa Maqsood Shiqi Liu Weiyan Hou liying zhang Zhenfei Wang School of Computer and Artificial Intelligence Zhengzhou UniversityZhengzhou450001China

Breast cancer,particularly Invasive Ductal Carcinoma(IDC),is a primary global health concern predominantly affecting women.Early and precise diagnosis is crucial for effective treatment planning.Several AI-based tech-niques for IDC-level classification have been proposed in recent years.Processing speed,memory size,and accuracy can still be improved for better performance.Our study presents ECAM,an Enhanced Channel-Wise Attention Mechanism,using deep learning to analyze histopathological images of Breast Invasive Ductal Carcinoma(BIDC).The main objectives of our study are to enhance computational efficiency using a Separable CNN architecture,improve data representation through hierarchical feature aggregation,and increase accuracy and interpretability with channel-wise attention mechanisms.Utilizing publicly available datasets,DataBioX IDC and the BreakHis,we benchmarked the proposed ECAM model against existing state-of-the-art models:DenseNet121,VGG16,and AlexNet.In the IDC dataset,the model based on AlexNet achieved an accuracy rate of 86.81%and an F1 score of 86.94%.On the other hand,DenseNet121 outperformed with an accuracy of 95.60%and an F1 score of 95.75%.Meanwhile,the VGG16 model achieved an accuracy rate of 91.20%and an F1 score of 90%.Our proposed ECAM model outperformed the state-of-the-art,achieving an impressive F1 score of 96.65%and an accuracy rate of 96.70%.The BreakHis dataset,the AlexNet-based model,achieved an accuracy rate of 90.82%and an F1 score of 90.77%.DenseNet121 achieved a higher accuracy rate of 92.66%with an F1 score of 92.72%,while the VGG16 model achieved an accuracy of 92.60%and an F1 score of 91.31%.The proposed ECAM model again outperformed,achieving an F1 score of 96.37%and an accuracy rate of 96.33%.Our model is a significant advancement in breast cancer diagnosis,with high accuracy and potential as an automated grading,especially for IDC.

关键词： Invasive ductal carcinoma breast cancer artificial intelligence deep learning

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The MKK3–MPK7 cascade phosphorylates ERF4 and promotes its rapid degradation to release seed dormancy in Arabidopsis

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Molecular Plant 2023年第11期16卷 1743-1758页

作者： Xi Chen Qiujia Li Ling Ding Shengnan zhang Siyao Shan Xiong Xiong Wenhui Jiang Bo Zhao liying zhang Ying Luo Yiming Lian Xiuqin Kong Xiali Ding Jun zhang Chunli Li Wim J.J.Soppe Yong Xiang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Center for Crop Science College of AgronomyQingdao Agricultural UniversityQingdao 266109China School of Petrochemical Engineering Lanzhou University of TechnologyLanzhou 730050China Hou Ji Laboratory in Shanxi Province Academy of AgronomyShanxi Agricultural UniversityTaiyuan 030031China State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing 400715China Rijk Zwaan 2678 ZG De Lierthe Netherlands

Seeds establish dormancy to delay germination until the arrival of a favorable growing season.In this study,we identify a fate switch comprised of the MKK3–MPK7 kinase cascade and the ethylene response factor ERF4 that is responsible for the seed state transition from dormancy to germination.We show that dormancy-breaking factors activate the MKK3–MPK7 module,which affects the expression of some a-EXPANSIN(EXPA)genes to control seed dormancy.Furthermore,we identify a direct downstream substrate of this module,ERF4,which suppresses the expression of these EXPAs by directly binding to the GCC boxes in their exon regions.The activated MKK3–MPK7 module phosphorylates ERF4,leading to its rapid degradation and thereby releasing its inhibitory effect on the expression of these EXPAs.Collectively,our work identifies a signaling chain consisting of protein phosphorylation,degradation,and gene transcription,by which the germination promoters within the embryo sense and are activated by germination signals from ambient conditions.

关键词： seed dormancy dormancy breaking seed germination MKK3 MPK7 ERF4 phosphorylation protein degradation DNA binding

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