Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster

作     者：Zhidi Wang Liying Zhang Xing Wang Zhidi Wang;Liying Zhang;Xing Wang

作者机构：College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Beijing Key Laboratory of Biodiversity and Organic FarmingBeijing 100193China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第125卷第3期

页      面：616-629页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the Natural Science Foundation of Beijing, China (No. 5212012) the National Natural Science Foundation of China (No. 31801190) the 2115 Talent Development Program of China Agricultural University 

主　　题：Drosophila melanogaster Silver nanoparticles(AgNPs) Reactive oxygen species(ROS) DNA damage RNA-seq Metallothionein 

摘      要：The widely use of silver nanoparticles(AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in ***, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila *** show here that AgNPs significantly decreased Drosophila fecundity, the third-instar larvae weight and rates of pupation and eclosion in a dose-dependent manner. AgNPs reduced fat body cell viability in MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)assays. AgNPs caused DNA damage in hemocytes and S2 cells. Interestingly, the mRNA levels of the entire metallothionein gene family were increased under AgNPs exposure as determined by RNA-seq analysis and validated by qRT-PCR, indicating that Drosophila responded to the metal toxicity of AgNPs by producing metallothioneins for detoxification. These findings provide a better understanding of the mechanisms of AgNPs toxicity and may provide clues to effect on other organisms, including humans.