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Electrocatalytic NAD(P)H regeneration for biosynthesis

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Green Chemical Engineering 2024年第1期5卷 1-15页

作者： Yaoxuan Li Guanhua Liu Weixi Kong Suoqing Zhang Yuemei Bao Hao Zhao Lihui Wang liya zhou Yanjun Jiang National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization School of Chemical Engineering and TechnologyHebei University of TechnologyTianjin300401China North China Pharmaceutical Group Corp Shijiazhuang050015China Tianjin Food Research Institute Tianjin301609China

The highly efficient chemoselectivity,stereoselectivity,and regioselectivity render enzyme catalysis an ideal pathway for the synthesis of various chemicals in broad applications.While the cofactor of an enzyme is necessary but expensive,the conversed state of the cofactor is not beneficial for the positive direction of the reaction.Cofactor regeneration using electrochemical methods has the advantages of simple operation,low cost,easy process monitoring,and easy product separation,and the electrical energy is green and sustainable.Therefore,bioelectrocatalysis has great potential in synthesis by combining electrochemical cofactor regeneration with enzymatic catalysis.In this review,we detail the mechanism of cofactor regeneration and categorize the common electron mediators and enzymes used in cofactor regeneration.The reaction type and the recent progress are summarized in electrochemically coupled enzymatic catalysis.The main challenges of such electroenzymatic catalysis are pointed out and future developments in this field are foreseen.

关键词： Biosynthesis Cofactor regeneration Bioelectrocatalysis NAD(P)H Electron mediator

FGF4 protects the liver from immune-mediated injury by activating CaMKKβ-PINK1 signal pathway to inhibit hepatocellular apoptosis

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1605-1623页

作者： Zhifeng Huang Tongtong Pan Liang Xu Lu Shi Xiong Ma liya zhou Luyao Wang Jiaojiao Wang Guoqing Zhu Dazhi Chen Lingtao Song Xiaomin Pan Xiaodong Wang Xiaokun Li Yongde Luo Yongping Chen Hepatology Diagnosis and Treatment Center Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Diseasesthe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)&School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China School of Laboratory Medicine and Life Sciences Wenzhou Medical University&Key Laboratory of Laboratory MedicineMinistry of EducationWenzhou Medical UniversityWenzhou 325035China Division of Gastroenterology and Hepatology Key Laboratory of Gastroenterology and HepatologyMinistry of HealthState Key Laboratory for Oncogenes and Related GenesRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Hangzhou Medical College Hangzhou 311300China

Immune-mediated liver injury (ILI) is a condition where an aberrant immune response due to various triggers causes the destruction of hepatocytes. Fibroblast growth factor 4 (FGF4) was recently identified as a hepatoprotective cytokine;however, its role in ILI remains unclear. In patients with autoimmune hepatitis (type of ILI) and mouse models of concanavalin A (ConA)- or S-100-induced ILI, we observed a biphasic pattern in hepatic FGF4 expression, characterized by an initial increase followed by a return to basal levels. Hepatic FGF4 deficiency activated the mitochondria-associated intrinsic apoptotic pathway, aggravating hepatocellular apoptosis. This led to intrahepatic immune hyper-reactivity, inflammation accentuation, and subsequent liver injury in both ILI models. Conversely, administration of recombinant FGF4 reduced hepatocellular apoptosis and rectified immune imbalance, thereby mitigating liver damage. The beneficial effects of FGF4 were mediated by hepatocellular FGF receptor 4, which activated the Ca^(2+)/calmodulin-dependent protein kinasekinase 2 (CaMKKβ) and its downstream phosphatase and tensin homologue-induced putative kinase 1 (PINK1)-dependent B-cell lymphoma 2-like protein 1-isoform L (Bcl-XL) signalling axis in the mitochondria. Hence, FGF4 serves as an early response factor and plays a protective role against ILI, suggesting a therapeutic potential of FGF4 and its analogue for treating clinical immune disorder-related liver injuries.

关键词： Fibroblast growthfactor 4 Fibroblast growth factor receptor 4 Immune liver injury Ca^(2+)/Calmodulin dependent protein

Preparation of hollow spherical covalent organic frameworks via Oswald ripening under ambient conditions for immobilizing enzymes with improved catalytic performance

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Nano Research 2023年第1期16卷 281-289页

作者： Hao Zhao Guanhua Liu Yunting Liu liya zhou Li Ma Ying He Xiaobing Zheng Jing Gao Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan 430062China

The hollow spherical covalent organic frameworks(COFs)have a wide application prospect thanks to their special structures.However,the controllable synthesis of uniform and stable hollow COFs is still a challenge.We herein propose a self-templated method for the preparation of hollow COFs through the Ostwald ripening mechanism under ambient conditions,which avoids most disadvantages of the commonly used hard-templating and soft-templating methods.A detailed time-dependent study reveals that the COFs are transformed from initial spheres to hollow spheres because of the inside-out Ostwald ripening process.The obtained hollow spherical COFs have high crystallinity,specific surface area(2,036 m^(2)·g^(−1)),stability,and single-batch yield.Thanks to unique hollow structure,clear through holes,and hydrophobic pore environment of the hollow spherical COFs,the obtained immobilized lipase(BCL@H-COF-OMe)exhibits higher thermostability,polar organic solvent tolerance,and reusability.The BCL@H-COF-OMe also shows higher catalytic performance than the lipase immobilized on non-hollow COF and free lipase in the kinetic resolution of secondary alcohols.This study provides a simple approach for the preparation of hollow spherical COFs,and will promote the valuable research of COFs in the field of biocatalysis.

关键词： hollow spherical structure covalent organic framework Oswald ripening mechanism lipase kinetic resolution

Enzyme@bismuth-ellagic acid:a versatile platform for enzyme immobilization with enhanced acid-base stability

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Frontiers of Chemical Science and Engineering 2023年第6期17卷 784-794页

作者： Junyang Xu Guanhua Liu Ying He liya zhou Li Ma Yunting Liu Xiaobing Zheng Jing Gao Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Hebei University of TechnologyTianjin 300130China

In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts;however,it is limited by the conditions of metal–organic framework synthesis and its acid-base stability.Herein,a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier.Bismuth-ellagic acid is a green phenol-based metal–organic framework whose organic precursor is extracted from natural plants.After encapsulation,the stability,especially the acid-base stability,of amyloglucosidases@bismuth-ellagic acid was enhanced,which remained stable over a wide pH range(2–12)and achieved multiple recycling.By selecting a suitable buffer,bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors,as well as between multiple enzymes.The green precursor,simple and convenient preparation process provided a versatile strategy for enzymes encapsulation.

关键词： bismuth-ellagic acid in situ encapsulation enzyme@MOF biocomposites

Visible light assisted enzyme-photocatalytic cascade degradation of organophosphorus pesticides

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Green Chemical Engineering 2023年第1期4卷 30-38页

作者： Ying Zhang Xue Cao Yufeng Yang Sumin Guan Xiaotian Wang Heyu Li Xiaobing Zheng liya zhou Yanjun Jiang Jing Gao School of Chemical Engineering and Technology Hebei University of TechnologyTianjin300130China Hebei Xinlan Environmental Protection Engineering Co.Ltd. Baoding071000China Tianjin UBasio Biotechnology Group Co. Ltd.Tianjin300457China National Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Hebei University of TechnologyTianjin300130China

The worldwide application of organophosphorus pesticides(OPs)has promoted agricultural development,but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals.Organophosphorus hydrolase(OPH)is an effective enzyme that can catalyze the degradation of the residual OPs.However,the degradation products such as p-nitrophenol(p-NP)is still toxic.Thus,it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of p-NP.Herein,a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO_(2)(named OPH@H-Au-TiO_(2))for the degradation of OPs.The obtained OPH@H-Au-TiO_(2)can degrade methyl parathion to p-NP by OPH and then degrade p-NP to hydroquinone with low toxicity by using H-Au-TiO_(2)under visible light.OPH molecules were immobilized on HAu-TiO_(2)through adsorption method to prepare OPH@H-Au-TiO_(2).After 2.5 h of reaction,methyl parathion is completely degraded,and about 82.64%of the generated p-NP is further degraded into hydroquinone.After reused for 4 times,the OPH@H-Au-TiO_(2)retains more than 80%of the initial degradation activity.This research presents a new insight in designing and constructing multi-functional biocatalyst,which greatly expands the application scenarios and industrial value of enzyme catalysis.

关键词： Organophosphorus pesticides Multi-functional biocatalyst Enzyme-photocatalytic cascade catalyst Immobilized enzyme Organophosphorus hydrolase

2022 Chinese national clinical practice guideline on Helicobacter pylori eradication treatment

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Chinese Medical Journal 2022年第24期135卷 2899-2910页

作者： liya zhou Hong Lu Zhiqiang Song Bin Lyu Ye Chen Jiyao Wang Jun Xia Zhan Zhao on behalf of Helicobacter Pylori Study Group of Chinese Society of Gastroenterology Department of Gastroenterology Peking University Third HospitalBeijing 100191China Department of Gastroenterology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Gastroenterology The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhouZhejiang 310018China Department of Gastroenterology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China Department of Gastroenterology Fudan University Affiliated Zhongshan HospitalShanghai 200032China Nottingham Ningbo GRADE Center Nottingham China Health InstituteUniversity of Nottingham Ningbo ChinaNingboZhejiang 315100China Academic Unit of Lifespan and Population Health School of MedicineThe University of NottinghamNottinghamUK Systematic Review Solutions Ltd Shanghai 201403China 不详

Background:Helicobacter pylori(H.pylori)infection is an infectious disease with a prevalence rate of up to 50%worldwide.It can cause indigestion,gastritis,peptic ulcer,and gastric cancer.H.pylori eradication treatment can effectively control disease progression and reduce the risk of the above conditions.However,the escalating trend of antibiotic resistance presents a global challenge for H.pylori eradication.We aim to provide guidance on pharmacological treatment of H.pylori infection.Methods:This clinical practice guideline is developed following the World Health Organization’s recommended process,adopting Grading of Recommendations Assessment,Development and Evaluation in assessing evidence quality,and utilizing Evidence to Decision framework to formulate clinical recommendations,minimizing bias and increasing transparency of the clinical practice guideline development process.We used the Reporting Items for practice Guidelines in HealThcare(RIGHT)statement and The Appraisal of Guidelines for Research and Evaluation Ⅱ(AGREE Ⅱ)as reporting and conduct guides to ensure the guideline’s completeness and transparency.Results:Though decreasing in developed countries,the prevalence of H.pylori remains high in developing countries,causing a major public health burden.This clinical practice guideline contains 12 recommendations concerning pharmacological treatment for H.pylori eradication.Among them,it is worth highlighting that bismuth preparations are inexpensive,safe,and effective,consequently making bismuth quadruple therapy a preferred choice for initial and rescue treatment.In empirical treatment,high-dose dual therapy is equally effective compared with bismuth quadruple therapy.Conclusions:The 12 recommendations in this clinical practice guideline are formed with consideration for stakeholders’values and preferences,resource use,feasibility,and acceptability.Recommendations are generalizable to resource limited settings with similar antibiotic resistance pattern as China,and lower middle-income countries facing

关键词： Helicobacter pylori Peptic ulcer Guideline Pharmacological treatment

幽门螺杆菌领域近十年临床研究进展和热点问题

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中华内科杂志 2024年第1期63卷 1-4页

作者：宋志强周丽雅北京大学第三医院消化科北京100191

幽门螺杆菌感染及其相关疾病是全球重要的卫生健康问题,尤其在我国幽门螺杆菌高感染率地区,影响人群广泛,消耗大量医疗资源。过去十年间,国内外在幽门螺杆菌领域取得了一系列研究进展,聚焦在多个研究热点,本文总结如下。

关键词：螺杆菌幽门消化系统疾病

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不同坐姿下的表面肌电特性及舒适度分析

家具与室内装饰 2024年第5期31卷 93-97页

作者：鲍洁周立亚东华大学服装与艺术设计学院上海200051 现代服装设计与技术教育部重点实验室上海200051

为了解不同坐姿下人体主要工作肌群的活跃度与疲劳情况,让人们重视对坐姿的调整,并处于舒适健康的坐姿状态,邀请8名受试者对6种日常坐姿分别进行表面肌电测试,收集颈伸肌、竖脊肌、腹直肌与腹外斜肌的表面肌电信号以及不同身体部位与整... 详细信息

为了解不同坐姿下人体主要工作肌群的活跃度与疲劳情况,让人们重视对坐姿的调整,并处于舒适健康的坐姿状态,邀请8名受试者对6种日常坐姿分别进行表面肌电测试,收集颈伸肌、竖脊肌、腹直肌与腹外斜肌的表面肌电信号以及不同身体部位与整体主观舒适度评价。结果表明:坐姿舒适度为:靠背直立>后倾>直立>前倾>跷二郎腿>单边坐。其中靠背直立坐姿与后倾坐姿的肌肉活跃度与肌力水平较低,主观舒适度较高。因此,建议大众在工作和学习时保持靠背直立坐姿,在短暂放松休息时可采用后倾坐姿。同时,从肌力平衡角度,在日常生活中注重腹肌肌群的锻炼,可缓解腰部肌肉的发力,减少脊柱患病的风险。最后,在坐姿状态下,颈伸肌与竖脊肌对不适较为敏感,因此在座椅设计与坐具舒适性研究中应该优先考虑颈部与腰部的设计合理性与舒适情况。

关键词：表面肌电坐姿肌肉活跃度舒适度

Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E.coli by macromolecular cross-linking agent

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Chinese Journal of Chemical Engineering 2017年第4期25卷 487-492页

作者： liya zhou Haixia Mou Jing Gao Li Ma Ying He Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving Hebei University of TechnologyTianjin 300130China

Cross-linked enzyme aggregates(CLEAs) of nitrile hydratase(NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time. In this process, egg white was added as protein feeder for facilitating the formation of CLEAs. The optimal conditions of the immobilization process were determined. Michaelis constants(Km) of free NHase and NHase CLEAs were also determined. The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart. When exposed to high concentrations of acrylamide, NHase CLEAs also exhibited effective catalytic activity.

关键词： Immobilization Enzyme Biocatalysis Nitrile hydratase