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ABA-mediated regulation of leaf and root hydraulic conductance in tomato grown at elevated CO2 is associated with altered gene expression of aquaporins

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Horticulture Research 2019年第1期6卷 397-406页

作者： Liang Fang Lamis Osama Anwar Abdelhakim Josefine Nymark Hegelund Shenglan Li Jie liu Xiaoying Peng Xiangnan Li Zhenhua Wei fulai liu Department of Plant and Environmental Sciences Faculty of ScienceUniversity of CopenhagenHøjbakkegaard Alle 132630 TaastrupDenmark Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas Ministry of EducationNorthwest A&F University712100 YanglingShaanxiChina college of Bioscience and Biotechnology Hunan Agricultural University410128 ChangshaHunanChina Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of Sciences130102 ChangchunChina Centre for Crop Systems Analysis Department of Plant SciencesWageningen University&ResearchPO Box 4306700 AK WageningenThe Netherlands

Elevated CO_(2)concentration in the air(e[CO_(2)])decreases stomatal density(SD)and stomatal conductance(gs)where abscisic acid(ABA)may play a role,yet the underlying mechanism remains largely *** investigated the effects of e[CO_(2)](800 ppm)on leaf gas exchange and water relations of two tomato(Solanum lycopersicum)genotypes,Ailsa Craig(WT)and its ABA-deficient mutant(flacca).Compared to plants grown at ambient CO_(2)(400 ppm),e[CO_(2)]stimulated photosynthetic rate in both genotypes,while depressed the gs only in *** showed a similar response to e[CO_(2)]as gs,although the change was not significant.e[CO_(2)]increased leaf and xylem ABA concentrations and xylem sap pH,where the increases were larger in WT than in *** leaf water potential was unaffected by CO_(2)growth environment,e[CO_(2)]lowered osmotic potential,hence tended to increase turgor pressure particularly for WT.e[CO_(2)]reduced hydraulic conductance of leaf and root in WT but not in flacca,which was associated with downregulation of gene expression of *** is concluded that ABA-mediated regulation of gs,SD,and gene expression of aquaporins coordinates the whole-plant hydraulics of tomato grown at different CO_(2)environments.

关键词： conductance hence ambient

Design and Experimental Testing of an Electric Field-Driven Droplet Injection Device

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Fluid Dynamics & Materials Processing 2023年第11期19卷 2891-2905页

作者： fulai Cao Yanpu Chao Hao Yi Shuai Lu Chengshui Guo college of Mechatronics Xuchang UniversityXuchang461000China State Key Laboratory of High Performance Complex Manufacturing Central South UniversityChangsha410083China State Key Laboratory of Mechanical Transmission Chongqing UniversityChongqing400044China Tobacco Sheet Manufacturing Department Henan Cigarette Industry Tobacco Sheet Limited CompanyXuchang461100China

The properties of droplets produced by existing on-demand injection systems are typically determined by the nozzle diameter,i.e.,only droplets with size larger than this diameter can be *** solve this problem,a system for electricfield-driven droplet injection and deposition was developed,and the related performances were compared with those of a standard pneumatic *** results show that the diameter of droplets gen-erated accordingly can be significantly smaller than the nozzle *** particular,the effects of frequency and duty ratio on the number of droplets were studied by assuming microcrystalline wax as work material.A deposi-tion matrix was obtained by using a nozzle with a diameter of 200μ*** average diameter of the matrix was 80.17μm with a standard deviation of 2.65μm and the average spacing was 301.25μm with a standard deviation of 6.80μ*** deposition states after droplet injection were considered and verified under different velocities of F60,F10,F3,and *** also provide an explanation for the existence of a series of inclined columns showing up during the deposition processs.

关键词： Electricfield-driven on-demand injection taylor cone droplet diameter deposition state

Nphos:Database and Predictor of Protein N-phosphorylation

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Genomics, Proteomics & Bioinformatics 2024年第3期22卷 139-151页

作者： Ming-Xiao Zhao Ruo-Fan Ding Qiang Chen Junhua Meng fulai Li Songsen Fu Biling Huang Yan liu Zhi-Liang Ji Yufen Zhao Institute of Drug Discovery Technology Ningbo UniversityNingbo 315211China Department of Chemical Biology Key Laboratory for Chemical Biology of Fujian ProvinceCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China State Key Laboratory of Cellular Stress Biology School of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China Zhejiang Key Laboratory of Pathophysiology Department of Biochemistry and Molecular BiologyHealth Science CenterNingbo UniversityNingbo 315211China BGI Genomics BGI-ShenzhenShenzhen 518083China Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology Department of ChemistryTsinghua UniversityBeijing 100084China

Protein N-phosphorylation is widely present in nature and participates in various biological ***,current knowledge on N-phosphorylation is extremely limited compared to that on *** this study,we collected 11,710 experimentally verified N-phosphosites of 7344 proteins from 39 species and subsequently constructed the database Nphos to share up-to-date information on protein *** these substantial data,we characterized the sequential and structural features of protein ***,after comparing hundreds of learning models,we chose and optimized gradient boosting decision tree(GBDT)models to predict three types of human N-phosphorylation,achieving mean area under the receiver operating characteristic curve(AUC)values of 90.56%,91.24%,and 92.01%for pHis,pLys,and pArg,***,we discovered 488,825 distinct N-phosphosites in the human *** models were also deployed in Nphos for interactive N-phosphosite *** summary,this work provides new insights and points for both flexible and focused investigations of *** will also facilitate a deeper and more systematic understanding of protein N-phosphorylation modification by providing a data and technical *** is freely available at http://***/Nphos/and http://***/Nphos/.

关键词： N-phosphorylation Post-translational modification Machine learning Database Benchmark dataset

Research on the Microstructure Construction Technology of Fully Degraded Polymer Vascular Stent Based on Electric Field Driven 3D Printing

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Fluid Dynamics & Materials Processing 2024年第11期20卷 2489-2508页

作者： Yanpu Chao fulai Cao Hao Yi Shuai Lu Yaohui Li Hui Cen college of Mechatronics Xuchang UniversityXuchang461000China college of Mechanical Engineering Chongqing UniversityChongqing400044China State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing400044China

The so-called fourth-generation biodegradable vascular stent has become a research hotspot in thefield of bioengineering because of its good degradation ability and drug-loading ***,the preparationof polymer-degraded vascular stents is affected by known problem such as poor processflexibility,low formingaccuracy,large diameter wall thickness,limited complex pore structure,weak mechanical properties of radial support and high process *** this study,a deposition technique based on a high-voltage electric-field-driven continuous rotating jet is proposed to fabricate fully degraded polymer vascular *** experimental results showthat,due to the rotation of the deposition axis,the deposition direction of PCL(polycaprolactone)micro-jet isalways tangent to the surface of the deposition *** direction of the viscous drag force is also consistent withthe deposition direction of the *** is shown that by setting different rotation speeds of deposition axisωandlinear motion speeds of the nozzle V,the direction of rotation,pitch and angle of the individual printed spiralcurve can be precisely *** the process of multiple spiral curves matching the deposition forming thin walltube mesh,the mesh shape and size of the thin wall tube can be accurately controlled by changing the number ofmatching spiral curves and the size of the matching position bias ***,the characteristics of a PCLtubular stent sample(with uniform-size microfibers and mesh shape),fabricated under the appropriate processparameters are described in detail.

关键词： Electricfield driven micro-jet spiral curve polymer vascular stent

Effect of volatile oil from Blumea Balsamifera(L.) DC. leaves on wound healing in mice

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Journal of Traditional Chinese Medicine 2014年第6期34卷 716-724页

作者： Yuxin Pang Dan Wang Xuan Hu Hui Wang Wanjin Fu Zuowang Fan Xiaolu Chen fulai Yu Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences Medicinal Efficacy Evaluation Laboratory Hainan Provincial Engineering Research Center for Ainaxiang (Herba Blumeae Balsamiferae) Key Laboratory of Tropical Crop Gene Resources and Germplasm Enhancement of Ministry of Agriculture college of Traditional Chinese Medicine Guangdong Pharmaceutical University college of Traditional Chinese Medicine Guangdong Pharmaceutical University

OBJECTIVE: To assess the effectiveness of violateoil from Blumea Balsamifera(L.) DC. leaves(BB oil)on wound healing in ***: Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield BB oil1/5and BB oil1/10 were applied to the wounded skin before wound healing conditions were assessed by healing rate, histopathology, and contents of collagen, hydroxyproline, and Neuropeptide Substance P(SP). All above results were compared with the efficacies of the control, pure olive oil, basic fibroblast growth factor(BFGF), and cream of Jing Wan Hong(JWH).RESULTS: BB oil1/5and BB oil1/10 improved wound contraction and closure. Histopathology study further confirmed a desirable histological organization of wound tissues. BB oil1/5and BB oil1/10 reduced the number of inflammatory cells, increased wound-healing rates, and significantly increased the hydroxyproline content. Both BB oil1/5and BB oil1/10 improved formation of collagen, and reduced the frequency of fibroblasts. Moreover, BB oil1/5and BB oil1/10 markedly promoted SP expression. However, undiluted BB oil may induce skin thickening and hardening, inhibite collagen synthesis and delay complete skin wound ***: The BB oil1/5and BB oil1/10 promoted capillary regeneration, blood circulation, collagen deposition, granular tissue formation, epithelial deposition, and wound contraction. The mechanism underlying the action might be related to induction of SP secretion, and the proliferation and differentiation of mesenchymal cells.

关键词： WoundBlumea Balsamifera (L.)cumhealing Neuropeptides DC. Borneolum Synthciticum

Development and utilization of a new chemically-induced soybean library with a high mutation density

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Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong liu Jinhui Lei Ying liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming liu Nang Myint Phyu Sin Htwe Lin liu Bingfu Guo Jian Song Bing Tan Guifeng liu Maiquan Li Xianli Zhang Bo liu Xuehui Shi Sining Han Sunan Hua fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research Institute Heilongjiang Academy of Agricultural Sciences college of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

EXISTENCE OF POSITIVE PERIODIC SOLUTION TO A CLASS OF NEUTRAL DELAY COMPETITION MODEL

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Annals of Differential Equations 2006年第1期22卷 13-20页

作者： Chen fulai Liao Jiawu Wen Xianzhang Dept. of Math. Xiangnan University Chenzhou 423000 college of Math. and Computer Science Hunan Normal University Changsha 410081

By means of the theory of coincidence degree, sufficient condition for the existence of positive periodic solution to certain neutral delay competition model is obtained.

关键词： neutral delay competition model periodic solution coincidence degree

NIR-Ⅱabsorbing organic nanoagents for photoacoustic imaging and photothermal therapy

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BMEMat(BioMedical Engineering Materials) 2023年第1期1卷 35-51页

作者： Wei Shao fulai Zhao Jinhong Xue Lingling Huang School of Engineering Westlake UniversityHangzhouChina Institute of Advanced Technology Westlake Institute for Advanced Study(WIAS)HangzhouChina School of Materials Science and Engineering Tianjin UniversityTianjinChina Jinci college Shanxi Medical UniversityTaiyuanChina Department of Pharmacy The Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina

Near-infrared(NIR)absorbing materials hold great potential in biomedical applications,such as fluorescence imaging(FLI),photoacoustic imaging(PAI),photodynamic therapy(PDT),and photothermal therapy(PTT).Generally,these materials can be classified into two main categories based on their absorbing wavelengths:the first NIR(NIR-I)(~650–950 nm)absorbing materials and the second NIR(NIR-Ⅱ)(~1000–1700 nm)absorbing *** to the reduced absorption and scattering of NIR-Ⅱlight in tissue compared to NIR-Ⅰlight,NIR-Ⅱabsorbing materials enable imaging and therapy with improved contrast and deepened penetration,which is in favor of practical *** inorganic materials have been developed for NIR-Ⅱphototheranostics in recent ***,the non-biodegradability and potential toxicity of these materials hinder their further clinical *** organic materials with potential biodegradability as well as tailored optical property are thus more *** this review,we sum-marize the recent advances of NIR-Ⅱabsorbing organic nanoagents(ONAs)based on small molecules(SMs)and conjugated polymers(CPs)for PAI and PTT and show our perspectives on future challenges and development of these materials.

关键词： NIR-Ⅱ organic nanoagents photoacoustic imaging phototheranostics photothermal therapy

OsiWRKY基因的水稻转化和转基因水稻抗病性分析

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浙江大学学报（农业与生命科学版） 2005年第6期31卷 697-700页

作者：李南羿柴荣耀 fulai Ran 郭泽建浙江大学生物技术研究所浙江杭州310029 浙江省农业科学研究院植物保护研究所浙江杭州310021 Dept.of Biology Queens college the City University of NewYork 中国农业大学农学与生物技术学院北京100094

为了研究水稻WRKY类基因的功能,对OsiWRKY基因进行了水稻转化.转基因植株的分子检测结果表明,OsiWRKY基因已整合到水稻基因组中并得到超量表达.进一步抗病性测定表明,转基因植株表现出对稻瘟病菌(Magnaporthe grisea)和白叶枯菌(Xanthom... 详细信息

为了研究水稻WRKY类基因的功能,对OsiWRKY基因进行了水稻转化.转基因植株的分子检测结果表明,OsiWRKY基因已整合到水稻基因组中并得到超量表达.进一步抗病性测定表明,转基因植株表现出对稻瘟病菌(Magnaporthe grisea)和白叶枯菌(Xanthomonas ***)的广谱抗性.这些结果说明OsiWRKY基因可能作为一种正调控因子参与水稻的抗病反应.

关键词： WRKY 转基因水稻稻瘟病菌白叶枯病菌抗病性