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Seedling Petri-dish inoculation method:A robust,easy-to-use and reliable assay for studying plant-Ralstonia solanacearum interactions

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Journal of Integrative Agriculture 2023年第12期22卷 3709-3719页

作者： CAO Peng CHEN Jia-lan LI Ning-ning ZHANG Shuang-xi WANG Rong-bo LI Ben-jin liu pei-qing AN Yu-yan ZHANG Mei-xiang National Engineering Laboratory for Endangered Medicinal Resource Development in Northwest China/Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry of Ministry of Education College of Life SciencesShaanxi Normal UniversityXi’an 710119P.R.China Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095P.R.China Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhou 350013P.R.China

Ralstonia solanacearum causes a lethal bacterial wilt disease in many crops,leading to huge losses in crop production every year.Understanding of plant-R.solanacearum interactions will aid to develop efficient strategies to control the disease.As a soilborne pathogen,R.solanacearum naturally infects plants via roots.A huge limitation in studying plant-R.solanacearum interactions is the large variation of R.solanacearum infection assay due to the variable soil conditions and uneven inoculum exposure.Here,we developed a robust and reliable Petri-dish inoculation method which allows consistent and stable infection in young plant seedlings.This method is easy to use,takes about only 10 days from seed germination to the completion of inoculation assay,and requires less inoculum of bacteria as well as growth chamber space.We proved the efficacy of the seedling Petri-dish inoculation method by analyzing plant defense primed by molecular patterns,resistance of defense-related plant mutants,and virulence of R.solanacearum mutants.Furthermore,we demonstrated that the seedling Petri-dish inoculation method can be applied to other host plants such as tobacco and has great potential for high-throughput screening of resistant plant germplasms to bacterial wilt in the future.

关键词： bacterial wilt Ralstonia solanacearum Petri-dish inoculation method virulence resistance defense priming

习近平关于消除相对贫困重要论述的理论意涵、生成逻辑与现实引领

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齐齐哈尔大学学报（哲学社会科学版） 2024年第4期 10-17页

作者：刘培卿中共四川省委党校(四川行政学院)科学社会主义教研部四川成都610072

绝对贫困的历史性消除,宣告我国进入相对贫困治理新阶段。新时代十年来,习近平围绕消除相对贫困形成了一系列重要论述,为我国反贫困事业指明了方向。其论述包蕴了四点理论意涵:相对贫困将长期存在,相对贫困群众的帮扶任务任重道远,相对... 详细信息

绝对贫困的历史性消除,宣告我国进入相对贫困治理新阶段。新时代十年来,习近平围绕消除相对贫困形成了一系列重要论述,为我国反贫困事业指明了方向。其论述包蕴了四点理论意涵:相对贫困将长期存在,相对贫困群众的帮扶任务任重道远,相对贫困治理是乡村振兴战略的重要任务和建立健全解决相对贫困的长效机制。其生成源于脱贫攻坚阶段奠定的实践基础、长期反贫困工作形成的思维方法和绝对贫困历史性解决的经验启益。在习近平相对贫困重要论述的现实引领下,现阶段相对贫困治理要遵循人民生活幸福是“国之大者”,同乡村振兴战略进行统筹部署,与实现人的全面发展互促共进,逐步实现全体人民共同富裕。

关键词：相对贫困绝对贫困贫困治理习近平新时代中国特色社会主义思想

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Comparative proteomic analysis of differentially expressed proteins between K562 and K562/ADM cells

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Chinese Medical Journal 2008年第5期121卷 463-468页

作者： SHEN Shao-hua GU Long-jun liu pei-qing YE Xin CHANG Wei-shan LI Ben-shang Shanghai Xinhua Hospital Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200127 China Laboratory of Pharmacology and Toxicology School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou Guangdong 510080 China Taian Central Hospital Taian Shandong 271000 China Vaccine and Infectious Disease Organization Saskatoon S7N5E3 Canada

Background Multidrug resistance to chemotherapeutic agents is an important clinical problem during the treatment of leukemia. The resistance process is multifactorial. To realize the total factors involved in multidrug resistance, we analyzed the differentially expressed proteins of K562 and K562/ADM cells and we investigated one of the up-regulated proteins （CRKL） using siRNA to determine its role in K562/ADM cells. Methods Altered protein expressions between K562/S （K562 ADM-sensitive cell line） and K562/ADM （K562 multidrug resistant cell line induced by adriamycin） were identified by 2D-DIGE coupled with mass spectrometry. Meanwhile, we confirmed the differential expression of CRKL and Stathmin in both K562 and K562/ADM cells by Western blot analysis. Furthermore, we used RNA interference to silence the CRKL gene expression. Results Among the 9 differentially expressed proteins, 3 were up-regulated in K562/ADM cells, while 6 were down-regulated in the K562/ADM cells compared with its parent cell line. The expression of CRKL was up-regulated significantly in K562/ADM cells, and it can be decreased by recombinant lentivirus. Moreover, the multidrug resistance of K562/ADM cells was efficiently reversed by silence of CRKL gene expression. Conclusions The data provided the differentially expressed proteins in K562 and its resistant cell line and highlights the power of 2D-DIGE for the discovery of resistance markers in cancer. We found CRKL may be a new protein involved in the multidrug resistanse of leukaemia cells.

关键词： multidrug resistance leukemia adriamycin proteomics

Berberine ameliorates renal injury in streptozotocin-induced diabetic rats by suppression of both oxidative stress and aldose reductase

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Chinese Medical Journal 2008年第8期121卷 706-712页

作者： liu Wei-hua HEI Zi-qing NIE Hong TANG Fu-tian HUANG He-qing LI Xue-juan DENG Yan-hui CHEN Shao-rui GUO Fen-fen HUANG Wen-ge CHEN Feng-ying liu pei-qing School of Pharmaceutical Science of Sun Yat-sen University Guangzhou Guangdong 510080 China Third Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510630 China Jinan University College of Pharmacy Guangzhou Guangdong 510632 China

Background Berberine is one of the main constituents of Coptidis rhizoma （CR） and Cortex phellodendri. In this study, we investigated the beneficial effects of berberine on renal function and its possible mechanisms in rats with diabetic nephropathy （DN）. Methods Male Wistar rats were divided into three groups： normal, diabetic model, and berberine treatment groups. Rats in the diabetic model and berberine treatment groups were induced to diabetes by intraperitonal injection with streptozotocin （STZ）. Glomerular area, glomerular volume, fasting blood glucose （FBG）, blood urea nitrogen （BUN）, serum creatinine （Cr） and urine protein for 24 hours （UP24h） were measured using commercially available kits. Meanwhile, the activity of superoxide dismutase （SOD）, content of malondialdehyde （MDA） in serum, activity of aldose reductase （AR） and the expression of AR mRNA and protein in kidney were detected by different methods. Results The results showed that oral administration of berberine （200 mg·kg^-1·d^-1） significantly ameliorated the ratio of kidney weight to body weight. Glomerular area, glomerular volume, FBG, BUN, Cr and UP24h were significantly decreased in the berberine treatment group compared with the diabetic model group （P〈0.05）. Berberine treatment significantly increased serum SOD activity and decreased the content of MDA compared with diabetic model group （P 〈0.05）. AR activity as well as the expression of AR mRNA and protein in the kidney was markedly decreased in the berberine treatment group compared with diabetic model group （P 〈0.05）. Conclusion These results suggested that berberine could ameliorate renal dysfunction in DN rats through controlling blood glucose, reduction of oxidative stress and inhibition of the activation of the polyol pathway.

关键词： berberine diabetic nephropathy oxidative stress aldose reductase polyol pathway

Emodin inhibits dietary induced atherosclerosis by antioxidation and regulation of the sphingomyelin pathway in rabbits

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Chinese Medical Journal 2006年第10期119卷 868-870页

作者： HEI Zi-qing HUANG He-qing TAN Hong-mei liu pei-qing ZHAO Ling-zhi CHEN Shao-rui HUANG Wen-ge CHEN Feng-ying GUO Fen-fen Department of Anesthesiology Third Affiliated Hospital SunYat-sen University Guangzhou 510630 China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510080 China Zhongshan Medical College Sun Yat-sen University Guangzhou510080 China

Emodin is an important component of rhubarb, a traditional Chinese herb. Previous studies in vitro showed that emodin inhibited the proliferation of human vascular smooth muscle cells, indicating the possible inhibitive effect of emodin on atherosclerosis. The present research evaluated the effect of emodin on the formation of atherosclerotic lesion induced by high cholesterol and fat diet in rabbits and the mechanism of this effect.

关键词： SIGNAL-TRANSDUCTION CERAMIDE

Cortactin/N-cadherin信号轴在病理性心肌肥大中的作用

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中国药理学通报 2024年第2期40卷 234-242页

作者：王钺镁喻文静孙袭孟张静路静刘培庆中山大学药学院广东广州510006

目的探究皮层肌动蛋白结合蛋白(Cortactin)对异丙肾上腺素(isoprenaline,ISO)诱导的病理性心肌肥大的调控作用及其机制。方法采用ISO刺激新生大鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)24 h,在细胞水平建立心肌肥大模型;C57BL/6... 详细信息

目的探究皮层肌动蛋白结合蛋白(Cortactin)对异丙肾上腺素(isoprenaline,ISO)诱导的病理性心肌肥大的调控作用及其机制。方法采用ISO刺激新生大鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)24 h,在细胞水平建立心肌肥大模型;C57BL/6小鼠皮下注射ISO 1周,在动物水平建立心肌肥大模型。采用RT-qPCR检测mRNA的变化;免疫印迹法检测相应蛋白含量的变化;免疫荧光法检测Cortactin的亚细胞定位及表达量的变化;采用腺病毒感染的方法过表达Cortactin,通过转染小干扰RNA敲低Cortactin。结果在细胞和动物水平上,成功建立ISO诱导的心肌肥大模型,均观察到ISO引起Cortactin和N型钙黏连蛋白(N-cadherin)水平降低;过表达Cortactin可逆转ISO导致的N-cadherin蛋白水平的降低及心肌细胞肥大反应;敲低Cortactin则显示相反的效应。结论Cortactin可能联合N-cadherin通过增强心肌细胞之间的连接,发挥抗心肌肥大的作用。

关键词：异丙肾上腺素心肌肥大心肌细胞肌动蛋白结合蛋白 N-cadherin 细胞连接

Isogeometric Analysis for the Arbitrary AFG Microbeam with Two-Phase Nonlocal Stress-Driven Model

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Acta Mechanica Solida Sinica 2024年第2期37卷 341-360页

作者： pei-Liang Bian Zhaowei liu Hai qing Tiantang Yu Department of Engineering Mechanics Hohai UniversityNanjing211100China State Key Laboratory of Mechanics and Control of Mechanical Structures Nanjing University of Aeronautics and AstronauticsNanjing210016China

Scale effects play critical roles in the mechanical responses of microstructures.An isogeometric analysis was developed here to investigate the mechanical responses of an axially functionally graded microbeam.The Euler–Bernoulli beam model was utilized,and size effects in the structure were modeled with a stress-driven two-phase local/nonlocal integral constitution.The governing equation of microstructures was given in an equivalent differential form with two additional constitutive boundary conditions.The framework was verified and utilized to analyze the microbeam’s static and dynamic mechanical responses.The present work showed great potential for modeling various types of functionally graded microstructures.

关键词： Isogeometric analysis Size effect Functionally graded material Nonlocal theory Euler-Bernoulli beam

S5A-4 SIRT3:A Potential Target for CHF?

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神经药理学报 2018年第4期8卷 80-81页

作者： YUE Zhong-bao YOU Jia LI Zhuo-ming CHEN Shao-rui liu pei-qing Department of Pharmacology and Toxicology School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhou510006China

Pathological cardiac hypertrophy is a maladaptive response in a variety of organic heart disease(OHD),which is characterized by mitochondrial dysfunction that results from disturbed energy metabolism.SIRT3,a mitochondria-localized sirtuin,regulates global mitochondrial lysine acetylation and preserves mitochondrial function.However,the mechanisms by which SIRT3 regulates cardiac hypertrophy remains to be further elucidated.In this study,we firstly demonstrated that expression of SIRT3 was decreased in AngiotensionⅡ(AngⅡ)-treated cardiomyocytes and in hearts of AngⅡ-induced cardiac hypertrophic mice.In addition,SIRT3 overexpression protected myocytes from hypertrophy,whereas SIRT3 silencing exacerbated AngⅡ-induced cardiomyocyte hypertrophy.In particular,SIRT3-KO mice exhibited significant cardiac hypertrophy.Mechanistically,we identified NMNAT3(nicotinamide mononucleotide adenylyltransferase 3),the rate-limiting enzyme for mitochondrial NAD biosynthesis,as a new target and binding partner of SIRT3.Specifically,SIRT3 physically interacts with and deacetylates NMNAT3,thereby enhancing the enzyme activity of NMNAT3 and contributing to SIRT3-mediated anti-hypertrophic effects.Moreover,NMNAT3 regulates the activity of SIRT3 via synthesis of mitochondria NAD.Taken together,these findings provide mechanistic insights into the negative regulatory role of SIRT3 in cardiac hypertrophy.Sirtuin 3(SIRT3),a mitochondrial deacetylase that may play an important role in regulating cardiac function and a potential target for CHF.

关键词： SIRT3 NMNAT3 chronic heart failure mitochondria energy metabolism

Numerical study of submerged bending vegetation under unidirectional flow

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Water Science and Engineering 2024年第1期17卷 92-100页

作者： pei-pei Zhang Yi-qing Gong Ken Vui Chua Jie Dai Jing-qiao Mao College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing 210098China Institute of Water Science and Technology Hohai UniversityNanjing 210098China School of Engineering Cardiff UniversityCardiff CF243AAUK

Submerged vegetation commonly grows and plays a vital role in aquatic ecosystems,but it is also regarded as a barrier to the passing flow.Numerical simulations of flow through and over submerged vegetation were carried out to investigate the effect of vegetation density on flow field.Numerical simulations were computationally set up to replicate flume experiments,in which vegetation was mimicked with flexible plastic strips.The fluid-structure interaction between flow and flexible vegetation was solved by coupling the two modules of the COMSOL packages.Two cases with different vegetation densities were simulated,and the results were successfully validated against the experimental data.The contours of the simulated time-averaged streamwise velocity and Reynolds stress were extracted to highlight the differences in mean and turbulent flow statistics.The turbulence intensity was found to be more sensitive to vegetation density than the time-averaged velocity.The developing length increased with the spacing between plants.The snapshots of the bending vegetation under instantaneous velocity and vorticity revealed that flexible vegetation responded to the effects of eddies in the shear layer by swaying periodically.The first two rows of vegetation suffered stronger approaching flow and were prone to more streamlined postures.In addition,the origin of tip vortices was investigated via the distribution of vorticity.The results reveal the variation of flow properties with bending submerged vegetation and provide useful reference for optimizationofrestorationprojects.

关键词： Computational fluid dynamics Fluid-structure interaction Turbulence Flexible and submerged vegetation