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Diethyl　Tartrate　on　Polystyrene　Bead　as　Chiral　Stationary　Phase　in　Liquid　Chromatography

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Chinese Chemical Letters 1996年第11期7卷 1035-1036页

作者： liu, QY Wu, XJ He, JG liu, gq CHINESE ACAD SCI INST CHEMBEIJING 100080PEOPLES R CHINA

L0-(+)-diethyl tartrate was bound to hydroxymethyl polystyrene bead as a chiral stationaryphase in high-performance liquid chromatography(HPLC) for the resolution of pharmaceuticallyracemic compounds, such as D,L-amyg... 详细信息

关键词： Tartrate Bead

氟苯尼考与三甲氧苄啶配伍应用对鸡大肠杆菌病的临床疗效试验

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中国兽药杂志 2006年第10期40卷 49-51页

作者：王春华刘国权锦州医学院畜牧兽医学院辽宁锦州121001

为了测定氟苯尼考与三甲氧苄啶(TMP)联合应用对临床鸡大肠杆菌病的疗效,以临床上对本地区大肠杆菌病有部分疗效的药物为对照组,分感染对照组、恩诺沙星组、氟苯尼考组和氟苯尼考与TMP配伍应用组。经对比治疗,验证氟苯尼考与TMP配伍应用... 详细信息

为了测定氟苯尼考与三甲氧苄啶(TMP)联合应用对临床鸡大肠杆菌病的疗效,以临床上对本地区大肠杆菌病有部分疗效的药物为对照组,分感染对照组、恩诺沙星组、氟苯尼考组和氟苯尼考与TMP配伍应用组。经对比治疗,验证氟苯尼考与TMP配伍应用组临床疗效较好,配伍比例为5∶1,药物浓度为50μg/mL,即每毫升的药液浓度含氟苯尼考42μg,TMP为8μg。

关键词：氟苯尼考 TMP 配伍大肠杆菌

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可溶性抗HAV抗体Fab片段的原核表达

第四军医大学学报 2001年第7期22卷 666-667页

作者：于长明宋宏斌刘国奇杜桂鑫徐志凯王海涛军事医学科学院微生物流行病研究所北京100071 第四军医大学微生物教研室

0　引言　甲型肝炎病毒（HAV）是危害人类健康的致病原之一，目前已有多种用于接触前预防的甲肝疫苗；但对于接触后预防或短期到HAV高流行区的旅游者的预防，则多采用免疫球蛋白作被动免疫. 在接触后（14 d内）和潜伏期早期，注射免疫... 详细信息

0　引言　甲型肝炎病毒（HAV）是危害人类健康的致病原之一，目前已有多种用于接触前预防的甲肝疫苗；但对于接触后预防或短期到HAV高流行区的旅游者的预防，则多采用免疫球蛋白作被动免疫. 在接触后（14 d内）和潜伏期早期，注射免疫球蛋白预防甲肝的有效率达85%左右. 血源性免疫球蛋白虽然效价高，应用效果好，但可能被血源病原体污染. 因此基因工程人源抗体有望成为接触后预防的首选制剂. 我们从人源噬菌体抗体组合文库中筛选到一株甲肝特异性抗体［1］，在此基础上构建了可溶性表达载体，并获得可溶性表达，为该抗体的进一步研究打下基础.1　材料和方法1.1　可溶性表达载体的构建　以SpeI，NheI双酶切噬菌体抗体表达载体质粒pComb3（本室保存），除去噬菌体包膜蛋白基因Ⅲ的片段，粘端自身环化，转化大肠杆菌XL1-Blue，得到可溶性Fab的表达载体psFab［2］.1.2　可溶性Fab在大肠杆菌中的表达　将37℃过夜培养的阳性菌体按10 g*L-1体积接种于含50 μg*L-1羧苄青霉素SB培养基30 g*L-1蛋白胨，20 g*L-1酵母提取物，10 g*L-1 MOPS），生长至 A600 nm=0.2～0.4，加入IPTG至1 mmol*L-1，30℃培养过夜，10 000 r*mim-1离心10 min，上清即含有Fab.1.3　免疫印迹　培养上清经SDS-PAGE后电转移到硝酸纤维素膜上，用HRP-羊抗人IgG Fab (Pierce)处理，DAB显色.

关键词：甲型肝炎病毒 Fab片段基因表达抗HAV抗体

POTENTIAL INVERSION PROBLEMS FOR COUPLED SYSTEM OF DOWNGOING AND UPCOMING ONE-WAY WAVE EQUATIONS

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Journal of Computational Mathematics 1998年第6期16卷 551-560页

作者： Zhang, gq Dou, XL Chinese Acad Sci ICMSEC State Key Lab Sci & Engn Comp Beijing 100080 Peoples R China Beijing Res Inst Remote Sensing Informat Beijing 100011 Peoples R China

By using wave splitting method the formulation of the two-dimensional potential inversion problem is set up in terms of the coupled system for downgoing and upcoming wavefields. The boundary counditions on the characteristic surface needed for solving the problem are derived by singularity analysis. Two stability theorems are given for the direct problems of the system treated as Cauchy problems in the direction of depth.

关键词： 2-D potential inversion wave splitting singularity analysis

LAYER STRIPPING METHOD FOR POTENTIAL INVERSION

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Journal of Computational Mathematics 1999年第1期17卷 25-32页

作者： Dou, XL Zhang, gq Chinese Acad Sci ICMSEC State Key Lab Sci & Engn Comp Beijing 100080 Peoples R China Beijing Res Inst Remote Sensing Informat Beijing 100011 Peoples R China

To solve the potential inversion problem of the coupled system for one-way wave equations, the absorbing boundary conditions in the lateral direction are derived. The difference schemes are constructed and a layer str... 详细信息

关键词： 2-D potential inversion layer stripping method absorbing boundary condition

Synthesis of the optically pure 5,5"-dihydroxy-7,7"-dimethoxy-8,8"-biflavone and its derivatives

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Chinese Journal of Chemistry 1997年第5期15卷 464-471页

作者： Zhang, FJ Lin, gq Chinese Acad Sci Shanghai Inst Organ Chem Shanghai 200032 Peoples R China

(+/-)-5,5 '-Dihydroxy-7,7 '-dimethoxy-8,8 '-biflavone ((+/-)-1) was resolved into its optically pure forms via the formation and recrystallization of its (2R)- and (2S)-1-(4-toluenesulfonyl)prolylate, and the methylated derivatives of (+)- and (-)-1 were also prepared. The absolute configurations of all these 8,8 '-biflavones have been confirmed by their CD spectra.

关键词： 8,8 ''-biflavones synthesis optical resolution absolute configuration CD spectra

Lipases catalyzed acetylation of meso-2,4-dimethyl-1,5-pentanediol and the synthesis of Prelog-Djerassi lactone

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Chinese Journal of Chemistry 1995年第4期13卷 380-384页

作者： LIN, gq XU, WC CHINESE ACAD SCI SHANGHAI INST ORGAN CHEMSHANGHAI 200032PEOPLES R CHINA

Lipase catalyzed acetylation of 2 afforded 3 with the e.e. up to 98%. Utility of 3 was illustrated by the preparation of Prelog-Djerassi lactones 7a and 7b.

关键词： LIPASE (2R,4S)-5-ACETOXY-2,4-DIMETHYL-PENTANOL PRELOG-DJERASSI LACTONE

A new bimetallic complex catalyst for carbonylation of methanol to acetic acid

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Chinese Science Bulletin 1997年第7期42卷 563-566页

作者： Wang, XJ Pan, PL liu, ZY Zhu, CC Yuan, gq Institute of Chemistry Chinese Academy of Sciences Beijing China

As the catalysts for carbonylation of methanol to acetic acid, the homogeneous complexes

关键词： bimetallic complex catalyst Iigand carbonylation of methanol.

Sealing Interfaces of Pin in Colour Picture Tube

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Journal of Materials Science & Technology 1996年第1期12卷 78-80页

作者： Wang, gq Tang, XY Ma, JS Dept. of Materials Science and Engineering Tsinghua University Beijing 100084 China

interfacial geometric morphology, chemistry and microstructure have been studied by SEM, EDAX, HREM, and XPS. It is found that the oxide scale/metal interface consists of two layers. The first one is amorphous SiO2 growing inward metal matrix, and the second one under the first layer is TiO2 distributing in metal matrix. There is microstructural transition zone at Cr2O3/SiO2 interface, and an atomic mixed zone with 8 nm width at SiO2/metal interface. The transition zone which is saturated with Cr diffusing from oxide scale exists at the oxide scale/glass interface. The reasons why sand-blasting process can improve bonding strength of the oxide scale and the metal are also discussed. The ideal interfaces are proposed finally.

关键词： Television picture tubes