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Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

Journal of Integrative Plant Biology 2023年第1期65卷 150-166页

作者： lisha zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China

The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ripening.However,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely unknown.Here,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening stage.Consistent with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the ripening.DNA affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB AM1.Stably overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ripening.Moreover,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation activity.Importantly,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by MaMsrA4.Collectively,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9.

关键词： banana EIN3/EIL redox regulation ROS starch degradation

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Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

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International Journal of Oral Science 2020年第1期12卷 10-24页

作者： lisha zhu Dan Luo Yan Liu Laboratory of Biomimetic Nanomaterials Department of OrthodonticsPeking University School and Hospital of StomatologyNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory of Digital StomatologyBeijingChina State Key Laboratory of Heavy Oil Processing College of New Energy and MaterialsBeijing Key Laboratory of Biogas Upgrading UtilizationChina University of Petroleum(Beijing)BeijingChina

Natural bone is a mineralized biological material, which serves a supportive and protective framework for the body, stores minerals for metabolism, and produces blood cells nourishing the body. Normally, bone has an innate capacity to heal from damage.However, massive bone defects due to traumatic injury, tumor resection, or congenital diseases pose a great challenge to reconstructive surgery. Scaffold-based tissue engineering(TE) is a promising strategy for bone regenerative medicine, because biomaterial scaffolds show advanced mechanical properties and a good degradation profile, as well as the feasibility of controlled release of growth and differentiation factors or immobilizing them on the material surface. Additionally, the defined structure of biomaterial scaffolds, as a kind of mechanical cue, can influence cell behaviors, modulate local microenvironment and control key features at the molecular and cellular levels. Recently, nano/micro-assisted regenerative medicine becomes a promising application of TE for the reconstruction of bone defects. For this reason, it is necessary for us to have in-depth knowledge of the development of novel nano/micro-based biomaterial scaffolds. Thus, we herein review the hierarchical structure of bone, and the potential application of nano/micro technologies to guide the design of novel biomaterial structures for bone repair and regeneration.

关键词： structure stores protective

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The SlTPL3–SlWUS module regulates multi-locule formation in tomato by modulating auxin and gibberellin levels in the shoot apical meristem

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Journal of Integrative Plant Biology 2022年第11期64卷 2150-2167页

作者： Shiwei Song Binbin Huang Zanlin Pan Qiuxiang Zhong Yinghua Yang Da Chen lisha zhu Guojian Hu Mi He Caiyu Wu Mohammed Zouine Riyuan Chen Mondher Bouzayen Yanwei Hao Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China Ministry of AgricultureCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Laboratory of Genomics and Biotechnology of Fruits INRAToulouse INPUniversity of ToulouseCastanet Tolosan F‐31326France

Malformed fruits depreciate a plant’s market value.In tomato(Solanum lycopersicum),fruit malformation is associated with the multi-locule trait,which involves genes regulating shoot apical meristem(SAM)development.The expression pattern of TOPLESS3(SITPL3)throughout SAM development prompted us to investigate its functional significance via RNA interference(RNAi)and clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(Cas9)-mediated gene editing.Lower SITPL3 transcript levels resulted in larger fruits with more locules and larger SAMs at the 5 d after germination(DAG5)stage.Differentially expressed genes in the SAM of wild-type(WT)and SITPL3-RNAi plants,identified by transcriptome deep sequencing(RNA-seq),were enriched in the gibberellin(GA)biosynthesis and plant hormone signaling pathways.Moreover,exogenous auxin and paclobutrazol treatments rescued the multi-locule phenotype,indicating that SITPL3 affects SAM size by mediating auxin and GA levels in the SAM.Furthermore,SITPL3 interacted with WUSCHEL(SIWUS),which plays an important role in SAM size maintenance.We conducted RNA-seq and DNA affinity purification followed by sequencing(DAP-seq)analyses to identify the genes regulated by SITPL3 and SIWUS in the SAM and to determine how they regulate SAM size.We detected24 overlapping genes regulated by SITPL3 and SIWUS and harboring an SIWUS-binding motif in their promoters.Furthermore,functional annotation revealed a notable enrichment for functions in auxin transport,auxin signal transduction,and GA biosynthesis.Dual-luciferase assays also revealed that SITPL3 enhances SIWUS-mediated regulation(repression and activation)of SIPIN3 and SIGA2 ox4 transcription,indicating that the SITPL3-SIWUS module regulates SAM size by mediating auxin distribution and GA levels,and perturbations of this module result in enlarged SAM.These results provide novel insights into the molecular mechanism of SAM maintenance and locule formation in tomato and highlight the SITPL3-SIWUS module as a key regula

关键词： auxin gibberellin locule number shoot apical meristem(SAM) TOPLESS3(Sl TPL3)

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Prim-O-glucosylcimifugin ameliorates aging-impaired endogenous tendon regeneration by rejuvenating senescent tendon stem/progenitor cells

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Bone Research 2023年第4期11卷 784-802页

作者： Yu Wang Shanshan Jin Dan Luo Danqing He Min Yu lisha zhu Zixin Li Liyuan Chen Chengye Ding Xiaolan Wu Tianhao Wu Weiran Huang Xuelin Zhao Meng Xu Zhengwei Xie Yan Liu Laboratory of Biomimetic Nanomaterials Department of OrthodonticsPeking University School and Hospital of Stomatology&National Center for Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental Materials&Translational Research Center for Orocraniofacial Stem Cells and Systemic HealthBeijing 100081China CAS Center for Excellence in Nanoscience Beijing Key Laboratory of Micro-nano Energy and SensorBeijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijing 101400China Peking University International Cancer Institute Health Science CenterPeking UniversityBeijing 100083China Department of Orthopedics the Fourth Medical Center of PLA General HospitalBeijing 100048China

Adult tendon stem/progenitor cells(TSPCs)are essential for tendon maintenance,regeneration,and repair,yet they become susceptible to senescence with age,impairing the self-healing capacity of tendons.In this study,we employ a recently developed deep-learning-based efficacy prediction system to screen potential stemness-promoting and senescence-inhibiting drugs from natural products using the transcriptional signatures of stemness.The top-ranked candidate,prim-O-glucosylcimifugin(POG),a saposhnikovia root extract,could ameliorate TPSC senescent phenotypes caused by long-term passage and natural aging in rats and humans,as well as restore the self-renewal and proliferative capacities and tenogenic potential of aged TSPCs.In vivo,the systematic administration of POG or the local delivery of POG nanoparticles functionally rescued endogenous tendon regeneration and repair in aged rats to levels similar to those of normal animals.Mechanistically,POG protects TSPCs against functional impairment during both passage-induced and natural aging by simultaneously suppressing nuclear factor-κB and decreasing mTOR signaling with the induction of autophagy.Thus,the strategy of pharmacological intervention with the deep learning-predicted compound POG could rejuvenate aged TSPCs and improve the regenerative capacity of aged tendons.

关键词： tendon endogenous progenitor

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高韧性PGA/TPEE共混物的制备及性能

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工程塑料应用 2024年第5期52卷 35-42页

作者：王梓冲吴亮朱丽莎徐鼐庞素娟潘莉莎海南大学材料科学与工程学院海口570228 海南大学海南省精细化工工程技术研究中心海口570228 海南大学化学化工学院海口570228

聚乙醇酸(PGA)具有高强度、高模量、良好的阻隔性能和生物相容性等优点,而针对其韧性差、加工成型稳定性不佳等问题进行改性,能进一步增强PGA材料的应用潜力。为此,将热塑性聚酯弹性体(TPEE)、多元环氧扩链剂(ADR)和PGA进行熔融共混,制... 详细信息

聚乙醇酸(PGA)具有高强度、高模量、良好的阻隔性能和生物相容性等优点,而针对其韧性差、加工成型稳定性不佳等问题进行改性,能进一步增强PGA材料的应用潜力。为此,将热塑性聚酯弹性体(TPEE)、多元环氧扩链剂(ADR)和PGA进行熔融共混,制备得到PGA/TPEE/ADR共混材料。其中,TPEE作为增韧组分,而ADR则作为反应性相容剂和扩链剂使用。通过转矩流变仪、熔体流动速率(MFR)仪、旋转流变仪、扫描电子显微镜、电子万能试验机、差示扫描量热仪、广角X射线衍射仪研究ADR的加入对PGA/TPEE(75/25)共混物的流变行为、微观结构、力学性能以及结晶性能等方面的影响。研究结果表明,随着ADR的加入,PGA/TPEE/ADR共混物的黏度和熔体强度上升、加工成型稳定性得到较大改善。当ADR添加量增大至0.9份时,PGA/TPEE/ADR(75/25/0.9)共混物的MFR值由PGA/TPEE(75/25)共混物的32.5 g/10 min降低至13.2 g/10 min。此外,ADR作为反应性相容剂,可明显改善PGA/TPEE界面相容性,共混物的韧性得到显著提升。PGA/TPEE/ADR(75/25/0.9)共混物的断裂伸长率和缺口冲击强度可由PGA/TPEE(75/25)共混物的54.6%和4.5 kJ/m^(2)分别增大至329.3%和16.1 kJ/m^(2)。

关键词：聚乙醇酸扩链增容流变行为力学性能

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Macrophage-derived small extracellular vesicles promote biomimetic mineralized collagen-mediated endogenous bone regeneration

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International Journal of Oral Science 2020年第4期12卷 317-326页

作者： Anqi Liu Shanshan Jin Cuicui Fu Shengji Cui Ting Zhang lisha zhu Yu Wang Steve G.F.Shen Nan Jiang Yan Liu Department of Oral and Maxillofacial Surgery Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of StomatologyShanghaiChina Laboratory of Biomimetic Nanomaterials Department of OrthodonticsPeking University School and Hospital of StomatologyNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory of Digital StomatologyBeijingChina Department of Oral Biochemistry Academic Centre for Dentistry Amsterdam(ACTA)University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU)Gustav Mahlerlaan 3004AmsterdamThe Netherlands Shanghai University of Medicine and Health Sciences ShanghaiChina Central Laboratory Peking University School and Hospital of StomatologyNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory of Digital StomatologyBeijingChina

Macrophages play an important role in material-related immune responses and bone formation,but the functionality of macrophage-derived extracellular vesicles(EVs)in material-mediated bone regeneration is still unclear.Here,we evaluated intracellular communication through small extracellular vesicles(sEVs)and its effects on endogenous bone regeneration mediated by biomimetic intrafibrillarly mineralized collagen(IMC).After implantation in the bone defect area,IMC generated more neobone and recruited more mesenchymal stem cells(MSCs)than did extrafibrillarly mineralized collagen(EMC).More CD63+CD90+and CD63+CD163+cells were detected in the defect area in the IMC group than in the EMC group.To determine the functional roles of sEVs,extracellular vesicles from macrophages cultured on different mineralized collagen were isolated,and they showed no morphological differences.However,macrophage-derived sEVs in the IMC group showed an enhanced Young’s modulus and exerted beneficial effects on the osteogenic differentiation of bone marrow MSCs by increasing the expression of the osteoblastic differentiation markers BMP2,BGLAP,COL1,and OSX and calcium nodule formation.Mechanistically,sEVs from IMC-treated macrophages facilitated MSC osteogenesis through the BMP2/Smad5 pathway,and blocking sEV secretion with GW4869 significantly impaired MSC proliferative,immunomodulative and osteogenic potential.Taken together,these findings show that macrophage-derived sEVs may serve as an emerging functional tool in biomaterial-mediated endogenous bone regeneration.

关键词： endogenous CD63 mediated

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浅谈审查意见答复策略的应用

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专利代理 2024年第2期 71-76页

作者：朱丽莎吴彬青常州至善至诚专利代理事务所(普通合伙) 常州213100

文章着重探讨了如何根据审查意见答复策略进行发明专利创造性的答复。发明专利创造性程度的高低是一个发明专利是否能够授权的关键所在,而基于创造性判断“三步法”是专利代理师进行审查意见创造性答复的根本。依据这个根本,专利代理师... 详细信息

文章着重探讨了如何根据审查意见答复策略进行发明专利创造性的答复。发明专利创造性程度的高低是一个发明专利是否能够授权的关键所在,而基于创造性判断“三步法”是专利代理师进行审查意见创造性答复的根本。依据这个根本,专利代理师围绕答复审查意见通知书的策略,按照事实认定、法律适用和意见陈述的判定标准,结合一件案例的两次审查意见进行深入分析、有效沟通和合理引导,最终实现了该发明专利的成功授权。

关键词：创造性答复专利技术启示

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将破解“钱学森之问”融入“大思政课”:意涵、挑战与路径

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未来与发展 2024年第2期48卷 71-74页

作者：魏崇辉朱丽莎上海交通大学上海200240

“大思政课”是培养人才的关键课程。对“钱学森之问”的破解是“大思政课”的基本使命。阐明破解“钱学森之问”的基本价值是“大思政课”首先需要面对的。基于此,“大思政课”要讲清楚破解“钱学森之问”的基本意涵、基本挑战和基本... 详细信息

“大思政课”是培养人才的关键课程。对“钱学森之问”的破解是“大思政课”的基本使命。阐明破解“钱学森之问”的基本价值是“大思政课”首先需要面对的。基于此,“大思政课”要讲清楚破解“钱学森之问”的基本意涵、基本挑战和基本实践。只有讲清楚破解“钱学森之问”的道理,才能有效践行习近平总书记“‘大思政课’我们要善用之”的重要指示。

关键词：钱学森之问大思政课科学家精神讲道理

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高效液相色谱法测定人血浆中伏立康唑浓度

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中国药业 2024年第13期33卷 56-58页

作者：朱丽莎苏强唐春梅四川省南充市中心医院·川北医学院第二临床医学院四川南充637000 个体化药物治疗南充市重点实验室四川南充637000

目的建立测定人血浆中伏立康唑浓度的高效液相色谱(HPLC)法。方法采用乙腈蛋白沉淀法对血液样本进行处理。色谱柱为Shimadzu InertSustain C18柱(250 mm×4.6 mm,5µm),流动相为甲醇-乙腈-磷酸盐缓冲液(33∶28∶39,V/V/V),流速为1.0 mL/... 详细信息

目的建立测定人血浆中伏立康唑浓度的高效液相色谱(HPLC)法。方法采用乙腈蛋白沉淀法对血液样本进行处理。色谱柱为Shimadzu InertSustain C18柱(250 mm×4.6 mm,5µm),流动相为甲醇-乙腈-磷酸盐缓冲液(33∶28∶39,V/V/V),流速为1.0 mL/min,柱温为30℃,检测波长为262 nm,进样量为20µL。结果伏立康唑的质量浓度在0.1~8µg/mL范围内与伏立康唑和酮康唑峰面积的比值线性关系良好(R2=0.9998,n=8);定量限为0.07µg/mL。低、中、高质量浓度(0.125,1,8µg/mL)样品的日内及日间精密度、冻融及长期稳定性试验结果的RSD均小于5%;提取回收率分别为(97.32±1.71)%,(97.61±1.22)%,(96.38±0.84)%。60例确诊为侵袭性真菌感染患者的血浆中,伏立康唑的质量浓度为0.278~9.129µg/mL。结论所建立的方法简单、快速、稳定性良好,适用于伏立康唑的血药浓度监测。

关键词：伏立康唑高效液相色谱法血药浓度

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Plant RNA-binding proteins:Phase separation dynamics and functional mechanisms underlying plant development and stress responses

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Molecular Plant 2024年第4期17卷 531-551页

作者： Sheng Fan Yu Zhang Shaobo zhu lisha Shen Temasek Life Sciences Laboratory 1 Research LinkNational University of Singapore1 Research LinkSingapore 117604Singapore Department of Biological Sciences National University of Singapore14 Science Drive 4Singapore 117543Singapore

RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in eukaryotes.Many RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA metabolism.Increasing evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress adaptation.Here,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA metabolism.We also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.

关键词： RNA-binding proteins phase separation biomolecular condensates RNA metabolism development stress resilience

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