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OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

Journal of Integrative Plant Biology 2024年第4期66卷 660-682页

作者： Ruiqing Li Yue Song Xueqiang Wang Chenfan Zheng Bo Liu Huali Zhang Jian Ke Xuejing wu liquan wu Ruifang Yang Meng Jiang College of Agronomy Anhui Agricultural UniversityHefei 230036China Hainan Institute Yazhou Bay Sci-Tech CityZhejiang UniversitySanya 572025China National Key Laboratory of Rice Biology Advanced Seed InstituteZhejiang UniversityHangzhou 311225China State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 311401China Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-Construction by Ministry and Province) Ministry of Agriculture and Rural AffairsCrop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghai 201106China

Due to its tropical origins,rice(Oryza sativa)is susceptible to cold stress,which poses severe threats to production.OsNAC5,a NAC-type transcription factor,participates in the cold stress response of rice,but the detailed mechanisms remain poorly understood.Here,we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5(OsABI5).Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold tolerance.OsNAC5 also enhanced OsABI5 stability,thus regulating the expression of cold-responsive(COR)genes,enabling fine-tuned control of OsABI5 action for rapid,precise plant responses to cold stress.DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression,including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A(OsDREB1A),OsMYB20,and PEROXIDASE 70(OsPRX70).In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription,with marked COR upregulation in OsNAC5-overexpressing lines and downregulation in osnac5 and/or osabi5 knockout mutants.This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module,which may be used to ameliorate cold tolerance in rice via advanced breeding.

关键词： ABA signaling ABI5 cold tolerance DREB NAC5 rice

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Effects of mixed fertilizers formed by the compounding of two targeted controlled-release nitrogen fertilizers on yield,nitrogen use efficiency,and ammonia volatilization in double-cropping rice

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The Crop Journal 2023年第2期11卷 628-637页

作者： Jian Ke Jie Sun Tingting Chen Shibao Tao Tiezhong Zhu Chuanjun Yin Haibing He Cuicui You liquan wu Shuangshuang Guo College of Agronomy Anhui Agricultural UniversityHefei 230031AnhuiChina Zoomlion Intelligent Agriculture Co. Ltd.Wuhu 241000AnhuiChina

One-time application of mixed fertilizer formed by the compounding of two controlled-release nitrogen fertilizers(CRUs)with targeted N supply during the periods from transplantation(TS)to panicle initiation(PI)and from PI to heading(HS)is expected to synchronize the double-peak N demand of rice.However,its effects on the yield and N use efficiency(NUE)of labor-intensive double-cropping rice were unknown.Two targeted CRU(CRU_(A)and CRU_(B))were compounded in five ratios(CRU_(A):CRU_(B)=10:0,7:3,5:5,3:7,and 0:10)to form five mixed fertilizers(BBFs):BBF1-5.A field experiment was performed to investigate the characteristics of N supply in early and late seasons under different BBFs and their effects on N uptake,yield,and ammonia volatilization(AV)loss from paddy fields of double-cropping rice.Conventional high-yield fertilization(CK,three split applications of urea)and zero-N treatments were established as controls.The N supply dropped significantly with the increased compound ratio of CRU_(B)during the period from TS to PI,but increased during the period from PI to HS.With the exception of the period from TS to PI in the late rice season,the N uptake of early and late rice maintained close synchronicity with the N supply of BBFs during the double-peak periods.Excessive N supply(BBF1 and BBF2)in the late rice season during the period from TS to PI increased N loss by AV.The effect of BBF on grain yield increase varied widely between seasons,irrespective of year.Among the BBFs,the BBF2 treatment of early rice not only stabilized the spikelets per panicle but also ensured a high number of effective panicles by promoting N uptake during the period from TS to PI and a high grain-filling percentage by appropriately reducing the N supply at the later PI stage,resulting in the highest rice yield.While stabilizing the effective panicle number,the BBF4 treatment of late rice increased the number of spikelets per panicle by promoting N uptake during the period from PI to HS,resulting in the highest rice yield.The two-year aver

关键词： Targeted controlled-release fertilizer Mixed fertilizer Double-cropping rice N uptake Yield

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经济修辞学研究的方法

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江苏师范大学学报（哲学社会科学版） 2024年第2期50卷 11-18页

作者：吴礼权复旦大学中国语言文学研究所上海200433

经济修辞学是一门新型学科,是基于经济学原理透视修辞现象及其规律的学问。无论是在西方,还是在中国,严格意义上的经济修辞学,迄今都尚未建立起来。正因为如此,对于经济修辞学的研究,我们必须重视采用科学的研究方法。唯有如此,才能从... 详细信息

经济修辞学是一门新型学科,是基于经济学原理透视修辞现象及其规律的学问。无论是在西方,还是在中国,严格意义上的经济修辞学,迄今都尚未建立起来。正因为如此,对于经济修辞学的研究,我们必须重视采用科学的研究方法。唯有如此,才能从经济学的视角透视语言表达中的诸多修辞现象,揭示修辞现象中蕴含的经济学原理,解释修辞现象发生的内在动因与发展规律。根据科学研究的基本规律,经济修辞学的研究可采用的研究方法主要有五种:归纳法、演绎法、观察法、问卷调查法与心理分析法。

关键词：经济修辞学研究方法归纳法演绎法观察法问卷调查法心理分析法

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集约投资行为与排比文本建构

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阜阳师范大学学报（社会科学版） 2024年第2期 36-43页

作者：吴礼权复旦大学中国语言文学研究所上海200433

在生产性经营与商业性经营活动中,都广泛存在着一种集约投资行为,它可以极大地提升生产性经营或商业性经营的经济效益。在语言活动中,也有类似于经济活动中的集约化投资行为,如以排比修辞手法建构排比文本,从经济学的视角看,虽然有超量... 详细信息

在生产性经营与商业性经营活动中,都广泛存在着一种集约投资行为,它可以极大地提升生产性经营或商业性经营的经济效益。在语言活动中,也有类似于经济活动中的集约化投资行为,如以排比修辞手法建构排比文本,从经济学的视角看,虽然有超量配置语言资源之嫌,似乎不符合“成本最小化”的经济原则,但就终极修辞效果来看,则符合“收益最大化”的经济原则。因为排比文本的建构,从接受效果上看,既实现了表意功能的最大化,又在表意功能之外别添了审美价值,可谓实现了“增殖效益”与“成本效益”最大化的完美统一。

关键词：集约投资排比文本资源配置成本效益增殖效益

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经济修辞学研究的意义

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淮北师范大学学报（哲学社会科学版） 2024年第2期45卷 26-29页

作者：吴礼权复旦大学中国语言文学研究所上海200433

“经济修辞学”的概念,虽然早在20世纪90年代就在西方学术界出现了,但是无论在西方还是在中国,立足“语言本位”,真正基于经济学的视角研究修辞活动的经济修辞学则并未真正展开。从学术发展的长远要求来看,亟需转换看问题的视角,基于“... 详细信息

“经济修辞学”的概念,虽然早在20世纪90年代就在西方学术界出现了,但是无论在西方还是在中国,立足“语言本位”,真正基于经济学的视角研究修辞活动的经济修辞学则并未真正展开。从学术发展的长远要求来看,亟需转换看问题的视角,基于“语言即资源”的观点,将修辞学与经济学打通,积极开展经济修辞学研究。经济修辞学的研究具有其特定的理论意义与实践意义,还因其交叉多元的特殊性,具有重要的学科建设意义。

关键词：经济修辞学研究理论意义实践意义学科建设意义

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土地生态服务价值估算与补偿标准研究——以云南怒江州为例

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国土资源科技管理 2024年第2期41卷 12-21页

作者：渠立权吴迪刘晨江苏师范大学地理测绘与城乡规划学院江苏徐州201116

本文以云南怒江州为研究对象,利用2020年的土地利用、经济、能源数据,综合运用生态服务功能价值法与碳排放法核算生态补偿标准,破解生态补偿中“补多少”的技术问题。结果显示:(1)2020年,怒江州土地生态服务价值为305.25亿元,其中林地... 详细信息

本文以云南怒江州为研究对象,利用2020年的土地利用、经济、能源数据,综合运用生态服务功能价值法与碳排放法核算生态补偿标准,破解生态补偿中“补多少”的技术问题。结果显示:(1)2020年,怒江州土地生态服务价值为305.25亿元,其中林地生态系统服务价值233.68亿元,占比达到76.55%;(2)2020年怒江州土地生态系统共吸收5619.934×10^(4)t碳,转换为经济价值为153.59亿元;(3)2020年怒江州生态补偿标准区间值为4.12亿元~11.19亿元,取区间均值作为应获得的生态补偿金较为合理,怒江州应获得的生态补偿标准总额为7.655亿元。

关键词：土地生态服务价值土地碳排放生态补偿标准云南怒江州

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Anode Interfacial Issues in Solid-State Li Batteries:Mechanistic Understanding and Mitigating Strategies

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Energy & Environmental Materials 2023年第4期6卷 384-404页

作者： Jiacheng Wang liquan Chen Hong Li Fan wu Tianmu Lake Institute of Advanced Energy Storage Technologies Liyang 213300China Henry Royce Institute and Department of Materials School of Natural SciencesThe University of ManchesterManchester M139PLUK Yangtze River Delta Physics Research Center Liyang 213300China Beijing Advanced Innovation Center for Materials Genome Engineering Key Laboratory for Renewable EnergyBeijing Key Laboratory for New Energy Materials and DevicesInstitute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Nano Science and Technology Institute University of Science and Technology of ChinaSuzhou 215123China

All-solid-state Li metal batteries(ASSLBs)using inorganic solid electrolyte(SE)are considered promising alternatives to conventional Li-ion batteries,offering improved safety and boosted energy density.While significant progress has been made on improving the ionic conductivity of SEs,the degradation and instability of Li metal/inorganic SE interfaces have become the critical challenges that limit the coulombic efficiency,power performance,and cycling stability of ASSLBs.Understanding the mechanisms of complex/dynamic interfacial phenomena is of great importance in addressing these issues.Herein,recent studies on identifying,understanding,and solving interfacial issues on anode side in ASSLBs are comprehensively reviewed.Typical issues at Li metal/SE interface include Li dendrite growth/propagation,SE cracking,physical contact loss,and electrochemical reactions,which lead to high interfacial resistance and cell failure.The causes of these issues relating to the chemical,physical,and mechanical properties of Li metal and SEs are systematically discussed.Furthermore,effective mitigating strategies are summarized and their effects on suppressing interfacial reactions,improving interfacial Li-ion transport,maintaining interfacial contact,and stabilizing Li plating/stripping are highlighted.The in-depth mechanistic understanding of interfacial issues and complete investigations on current solutions provide foundations and guidance for future research and development to realize practical application of high-performance ASSLB.

关键词： all-solid-state Li metal batteries anode interfacial issues interface protection and modification interfacial reaction and evolution li dendrite growth

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圈养马麝的攻击性与粪样类固醇激素水平及麝香分泌的关系

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兽类学报 2024年第3期44卷 344-350页

作者：王晓龙吴佳忆周鑫吕青昕申立泉耿硕孟秀祥中国人民大学生态环境学院北京100872 西藏大学生态环境学院拉萨850000

动物行为的发育和表达受激素调控,动物的攻击性等个性特征与其激素水平关系紧密,并影响其生长和繁殖。本研究于2021年10月2日至12月15日采用焦点动物取样法和全事件记录法对兴隆山马麝繁育中心53头圈养雄性马麝(Moschus chrysogaster)... 详细信息

动物行为的发育和表达受激素调控,动物的攻击性等个性特征与其激素水平关系紧密,并影响其生长和繁殖。本研究于2021年10月2日至12月15日采用焦点动物取样法和全事件记录法对兴隆山马麝繁育中心53头圈养雄性马麝(Moschus chrysogaster)进行行为取样,结合粪样类固醇激素水平检测,分析雄麝的攻击性与粪样皮质醇水平、睾酮水平及麝香分泌的关系。结果表明:马麝的攻击性与粪样皮质醇水平呈显著正相关(r=0.311,P=0.023),攻击性强的马麝个体皮质醇水平(60.41±2.82)ng/g显著高于攻击性弱的个体(49.35±4.32)ng/g;攻击性与粪样睾酮水平呈显著正相关(r=0.326,P=0.017),攻击性强的马麝个体睾酮水平(101.27±4.94)ng/g显著高于攻击性弱的个体(84.48±4.10)ng/g;攻击性与麝香分泌量之间呈显著负相关(r=−0.282,P=0.041),攻击性弱的马麝个体麝香分泌量(10.62±0.58)g显著高于攻击性强的个体(8.28±0.91)g;攻击性与麝香酮含量之间呈显著负相关(r=−0.285,P=0.039),攻击性弱的马麝个体麝香酮含量(1.98±0.18)%显著高于攻击性强的个体(1.31±0.23)%。基于本研究结果,在麝类驯养实践中,建群时要避免较强攻击性个体同处一群,并基于攻击性的快速评估及粪样激素的实时监测等,即时优化圈养麝的社群结构,减少麝群的社群胁迫,提升个体的麝香分泌。本研究结果可为麝类迁地保育的优化及药用麝香的可持续生产提供参考。

关键词：马麝攻击性粪样类固醇激素麝香分泌麝香酮

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Expression Analysis of Heat Shock Protein 70 Gene in Rice(Oryza sativa L.)

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Agricultural Biotechnology 2022年第1期11卷 1-4,21页

作者： Fei TONG Jiwei LIU Yuanna CHENG Ting YE Zhaodong LIU Ni ZHAN Hui YE liquan wu Juan LI College of Agriculture Anhui Agricultural UniversityHefei 230036China College of Life Science Anhui Agricultural UniversityHefei 230036China Biotechnology Center Anhui Agricultural UniversityHefei 230036China

[Objectives]This study was conducted to construct a pColdI-HSP70 recombinant prokaryotic expression vector.[Methods]With rice‘N22’as the test variety,hydroponic experiments were set up for rice seedlings with normal growth(CK),high temperature treatment(H),drought treatment(D)and drought-high temperature cross treatment(DH).The recombinant prokaryotic expression vector was constructed by the method of prokaryotic expression,and its induction expression time,IPTG concentration and temperature were optimized.[Results]The pColdI-HSP70 expression vector was successfully constructed,and the fusion protein was highly expressed in host strain BL21,and the expressed proteins were all in a soluble form.By optimizing the induction expression conditions,it was found that the optimal expression conditions were the IPTG concentration of 0.5 mmol/L and induction at 20℃for 36 h.The expression analysis of the rice HSP70 gene under different stress treatments was carried out by qRT-PCR technology,and it was found that H,D and DH stresses all could induce its expression,and its expression levels were 4.65,1.40 and 17.66 times higher than that of the CK group,respectively.[Conclusions]This study lays a solid foundation for the isolation,purification and functional study of rice HSP70 proteins.

关键词： Rice HSP70 qRT-PCR Prokaryotic expression

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Air Stability of Solid‑State Sulfide Batteries and Electrolytes

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Electrochemical Energy Reviews 2022年第3期5卷 213-258页

作者： Pushun Lu Dengxu wu liquan Chen Hong Li Fan wu Tianmu Lake Institute of Advanced Energy Storage Technologies Liyang 213300JiangsuChina Yangtze River Delta Physics Research Center Liyang 213300JiangsuChina Beijing Advanced Innovation Center for Materials Genome Engineering Key Laboratory for Renewable EnergyBeijing Key Laboratory for New Energy Materials and DevicesInstitute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

Sulfides have been widely acknowledged as one of the most promising solid electrolytes(SEs)for all-solid-state batteries(ASSBs)due to their superior ionic conductivity and favourable mechanical properties.However,the extremely poor air stability of sulfide SEs leads to destroyed structure/performance and release of toxic H_(2)S gas,which greatly limits mass-production/practical application of sulfide SEs and ASSBs.This review is designed to serve as an all-inclusive handbook for studying this critical issue.First,the research history and milestone breakthroughs of this field are reviewed,and this is followed by an in-depth elaboration of the theoretical paradigms that have been developed thus far,including the random network theory of glasses,hard and soft acids and bases(HSAB)theory,thermodynamic analysis and kinetics of interfacial reactions.Moreover,the characterization of air stability is reviewed from the perspectives of H2S generation,morphology evolution,mass change,component/structure variations and electrochemical performance.Furthermore,effective strategies for improving the air stabilities of sulfide SEs are highlighted,including H_(2)S absorbents,elemental substitution,design of new materials,surface engineering and sulfide-polymer composite electrolytes.Finally,future research directions are proposed for benign development of air stability for sulfide SEs and ASSBs.

关键词： Sulfide solid electrolytes Air stability Superionic conductors All-solid-state batteries

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