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Aptamer-Based Cell-Surface Profiling with Single-Cell Resolution Enables Precise Cancer Characterization

CCS Chemistry 2024年第1期6卷 196-207页

作者： Liujun Xu Yawei Feng Tong Wang Shenhuan Li Kangli Xu Yue Sun Yi Luo Yishan Ye Yan Miao Yun Dong Zhenzhen Guo Qing Zhang Benshang Li He Huang Xue-Qiang Wang liping qiu Weihong Tan Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082 The Key Laboratory of Zhejiang Province for Aptamers and Theranostics Zhejiang Cancer HospitalHangzhou Institute of MedicineChinese Academy of SciencesHangzhouZhejiang 310022 Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240 Bone Marrow Transplantation Center The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003 Institute of Hematology Zhejiang UniversityHangzhou 310003 Department of Hematology and Oncology Shanghai Children’s Medical CenterShanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200127

Molecular profiling of cell-surface proteins is a powerful strategy for precise cancer *** mass cytometry(MC)enables synchronous detection of over 40 cellular parameters,its full potential in disease classification is challenged by the limited types of recognition probes currently *** this work,we synthesize a panel of heavy isotopeconjugated aptamers to profile cancer-associated signatures on the surface of hematological malignancy(HM)*** on 15 molecular signatures,we performed cell-surface profiling that allowed the precise classification of 8 HM cell *** with machine-learning technology,this aptamer-based MC platform also achieved multiclass identification of HM subtypes in clinical sampleswith 100%accuracy in the training cohort and 80%accuracy in the test ***,we report an effective and practical strategy for precise cancer classification at the singlecell level,paving the way for its clinical use in the near future.

关键词： molecular profiling cancer diagnosis mass cytometry aptamers machine learning

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南方典型红壤区马尾松生物量空间特征及驱动力研究

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西南林业大学学报（自然科学） 2024年第1期44卷 106-115页

作者：丘丽萍张帆林嘉源徐伟涛赖日文福建农林大学林学院福建福州350002 中共漳浦县委组织部福建漳州363200

基于长汀县森林资源二类调查数据估算马尾松地上生物量,使用全局Moran’s I指数及热点(Getis–Ord G_(i)^(*))进行空间特征分析;运用地理探测器探究其影响因子,构建结构方程模型阐明其驱动机制。结果表明:研究区马尾松林分地上生物量为5... 详细信息

基于长汀县森林资源二类调查数据估算马尾松地上生物量,使用全局Moran’s I指数及热点(Getis–Ord G_(i)^(*))进行空间特征分析;运用地理探测器探究其影响因子,构建结构方程模型阐明其驱动机制。结果表明:研究区马尾松林分地上生物量为53.563 t/hm^(2),存在明显的空间分布差异,空间特征表现为研究区西部、北部的高值聚类和集中在中南部的低值聚类。郁闭度、海拔、林分年龄、年均气温、降水量、土壤有机质和土壤全氮含量为影响研究区马尾松生物量空间分异较显著的影响因子,双因子交互作用后解释力得到增强。结构方程模型表明驱动路径的主要因子为郁闭度、林分年龄、海拔和年均降水。郁闭度通过直接效应影响生物量,海拔通过影响降水和气温从而对林分生物量造成间接影响,林龄和年均降水量对生物量既有直接效应又有间接效应,林龄通过影响郁闭度产生间接效应,年均降水量通过影响土壤全氮产生间接效应。综上所述,长汀县马尾松地上生物量呈现显著的空间聚类分布特征,研究区中南部区域为今后马尾松林分质量和生态治理成效进一步提升的重点区域,今后长汀县马尾松的经营管理可以通过优化林分结构、提高郁闭度和改善土壤养分等措施提升马尾松林分质量。

关键词：地上生物量空间特征地理探测器结构方程模型马尾松

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Microstructure and mechanical properties stability of pre-hardening treatment in Al-Cu alloys for pre-hardening forming process

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International Journal of Minerals,Metallurgy and Materials 2024年第3期31卷 539-551页

作者： liping Tang Pengfei Wei Zhili Hu qiu Pang Hubei Longzhong laboratory Xiangyang 441000China Hubei Key Laboratory of Advanced Technology for Automotive Components School of Automotive EngineeringWuhan University of TechnologyWuhan 430070China Hubei Engineering Research Center for Green&Precision Material Forming Wuhan University of TechnologyWuhan 430070China Key Laboratory of Metallurgical Equipment and Control Technology of Ministry of Education School of Machinery and AutomationWuhan University of Science and TechnologyWuhan 430081China

The stability of the microstructure and mechanical properties of the pre-hardened sheets during the pre-hardening forming(PHF)process directly determines the quality of the formed *** microstructure stability of the pre-hardened sheets was in-vestigated by differential scanning calorimetry(DSC),transmission electron microscopy(TEM),and small angle X-ray scattering(SAXS),while the mechanical properties and formability were analyzed through uniaxial tensile tests and formability *** results in-dicate that the mechanical properties of the pre-hardened alloys exhibited negligible changes after experiencing 1-month natural aging(NA).The deviations of ultimate tensile strength(UTS),yield strength(YS),and sheet formability(Erichsen value)are all less than 2%.Also,after different NA time(from 48 h to 1 month)is applied to alloys before pre-hardening treatment,the pre-hardened alloys possess stable microstructure and mechanical properties as ***,with the extension of NA time before pre-hardening treatment from 48 h to 1 month,the contribution of NA to the pre-hardening treatment is *** a yield strength increment of 20 MPa is achieved,with no loss in *** limited enhancement is mainly attributed to the fact that only a limited number of clusters are transformed into Guinier-Preston(GP)zones at the early stage of pre-hardening treatment,and the formation ofθ''phase inhibits the nucleation and growth of GP zones as the precipitated phase evolves.

关键词： Al-Cu alloy pre-hardened alloy natural aging mechanical properties microstructure

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菌藻协同对大口黑鲈养殖水环境、生长和免疫酶活性的影响

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大连海洋大学学报 2024年第1期39卷 29-37页

作者：曾天宇陈曦徐慧敏孟顺龙金渝钦胡庚东李丹丹裘丽萍宋超范立民徐跑上海海洋大学水产与生命学院上海201306 中国水产科学研究院淡水渔业研究中心农业农村部水产品质量安全环境因子风险评估实验室(无锡) 中国水产科学研究院内陆渔业生态环境和资源重点开放实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

为探索添加小球藻(Chlorella vulgaris)、枯草芽孢杆菌(Bacillus subtilis)和小球藻+枯草芽孢杆菌对大口黑鲈(Micropterus salmoides)养殖水环境、生长性能及其相关酶活性的影响,在1000 L聚乙烯桶中进行试验,共设置空白组(饲喂基础饲料... 详细信息

为探索添加小球藻(Chlorella vulgaris)、枯草芽孢杆菌(Bacillus subtilis)和小球藻+枯草芽孢杆菌对大口黑鲈(Micropterus salmoides)养殖水环境、生长性能及其相关酶活性的影响,在1000 L聚乙烯桶中进行试验,共设置空白组(饲喂基础饲料)、小球藻组、枯草芽孢杆菌组和菌藻混合组(水体中同时添加小球藻和枯草芽孢杆菌)4个组,每组设3个重复,每个重复放50尾体质量为(11.71±0.90)g的大口黑鲈,进行60 d的养殖试验。结果表明:试验过程中,与空白组相比,加藻/菌试验组水体中所测理化指标总体上均有所优化,各试验组总氮、总磷和溶解氧含量差异较小,而氨氮、硝酸盐氮和亚硝酸盐氮含量相差较大,尤其是菌藻混合组水体中的氨氮、硝酸盐氮和亚硝酸盐氮含量在多数时间点均达到了最低水平(P<0.05);试验结束时,各试验组大口黑鲈的体质量、增重率和特定生长率均较空白组有所增加,但仅菌藻混合组显著高于空白组(P<0.05);试验过程中,与空白组相比,加藻/菌试验组鱼肝脏还原型谷胱甘肽(GSH)含量、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)和溶菌酶(LZM)活力总体上均显著提高(P<0.05),丙二醛(MDA)含量显著降低(P<0.05),尤其是菌藻混合组的抗氧化效果更好。研究表明,在水环境中添加小球藻或枯草芽孢杆菌均可以改善水质环境,促进大口黑鲈生长,提高其抗氧化和免疫能力,藻菌协同效果更优。

关键词：大口黑鲈小球藻枯草芽孢杆菌水质生长性能

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以草莓花梗为外植体的高效组培快繁技术

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浙江农业科学 2024年第7期65卷 1614-1617页

作者：蔡鑫江景勇陈银龙王娇阳米敏邱莉萍台州市农业科学研究院浙江台州318000

健康种苗是草莓产业的重要组成部分,而组培快繁技术是生产健康种苗的重要一环。文章通过比较不同品种来源、不同器官来源外植体产生的不定芽发生率和再生芽数,来探究草莓花梗成苗的快繁技术。试验发现,带有花蕾和苞片部位的花梗能在接种... 详细信息

健康种苗是草莓产业的重要组成部分,而组培快繁技术是生产健康种苗的重要一环。文章通过比较不同品种来源、不同器官来源外植体产生的不定芽发生率和再生芽数,来探究草莓花梗成苗的快繁技术。试验发现,带有花蕾和苞片部位的花梗能在接种后3周形成不定芽,6周形成丛生芽;不定芽发生率可达100%。这为草莓种质资源保存、健康种苗生产提供了有力保障。

关键词：草莓花梗苞片不定芽匍匐茎组织培养

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DNA-nanostructure-templated precise biomineralization

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National Science Review 2018年第6期5卷 789-791页

作者： liping qiu Hui Liu Jianhui Jiang Weihong Tan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Bio-sensing and Chemometrics College of Chemistry and Chemical Engineering and College of Biology Institute of Molecular Medicine Renji HospitalSchool of Medicine and College of Chemistry and Chemical Engineering Shanghai Jiao Tong University

As the best designer in material science,nature has developed unique strategies for synthesizing various ingenious materials with highly controlled structural features and outstanding biophysical properties to fit dif... 详细信息

关键词： DNA-nanostructure-templated precise biomineralization flexible

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Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease

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Journal of Oceanology and Limnology 2020年第2期38卷 509-516页

作者： ZOU Dandan TANG Xianghai qiu liping MAO Yunxiang College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

To explore ef fective physiological indexes to distinguish the resistant lines and susceptible lines of Pyt hium porphyrae,the causal agent of red rot disease of Pyropia yezoensis,and establish the disease-resistance breeding strategy,we obtained and analyzed the candidate resistant and susceptible lines by population *** of the candidate lines were cultured in seawater containing *** *** activities,including superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO),were measured and compared between the two lines before and after *** the resistant lines,SOD and POD activities increased and then decreased,but PPO activity rose with the prolongation of the infection *** phenylalanine ammonia lyase(PAL)activities also increased and then decreased after infection,but it had significantly different expression in the two lines without pathogen *** synthesis rates ofβ-1,3-glucanase,and cell-wall degrading enzyme were different from each other between the two lines after infection of P *** in the contents of malondialdehyde(MDA)and reactive oxygen species(ROS)in the two lines showed that,the two contents varied synchronously in response to the pathogen *** of these enzymes activities or contents demonstrated that *** could resist against the pathogen of P *** with the antioxidant defense *** addition,β-1.3-glucanase content showed extremely significant dif ference between the two lines,and the PAL had consistent expression dif ***,phenylalanine ammonia lyase(PAL)andβ-1,3-glucanase can be considered as an ef fective index to distinguish susceptible line and resistant line,with which the workload of the resistant breeding could be reduced in the future.

关键词： population selection resistant lines susceptible lines P yropia yezoensis Pythium porphyrae

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T/CALAS 100—2021《实验动物绿色实验动物设施评价》编制介绍

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暖通空调 2024年第5期54卷 130-133页

作者：王清勤赵力范东叶吴伟伟仇丽娉王博雅范红亚中国建筑科学研究院有限公司北京

本文介绍了T/CALAS 100—2021《实验动物绿色实验动物设施评价》标准的编制背景、主要技术内容、评价方法与等级划分、评价指标体系、主要特点等方面内容。该标准以完善绿色建筑评价标准体系,推动实验动物设施绿色发展、节约资源、保护... 详细信息

本文介绍了T/CALAS 100—2021《实验动物绿色实验动物设施评价》标准的编制背景、主要技术内容、评价方法与等级划分、评价指标体系、主要特点等方面内容。该标准以完善绿色建筑评价标准体系,推动实验动物设施绿色发展、节约资源、保护环境为目标,对实验动物设施全寿命周期内五大性能的综合评价进行约束和引导。

关键词：实验动物绿色实验动物设施评价方法评价指标体系全寿命周期

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Characterization of Pythium chondricola associated with red rot disease of Pyropia yezoensis (Ueda)(Bangiales,Rhodophyta) from Lianyungang,China

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Journal of Oceanology and Limnology 2019年第3期37卷 1102-1112页

作者： qiu liping MAO Yunxiang TANG Lei TANG Xianghai MO Zhaolan Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyKey Laboratory of Marine Genetics and Breeding (Ocean University of China)Ministry of EducationQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyKey Laboratory of Maricultural Organism Disease ControlMinistry of Agriculture and Rural AffairsQingdao Key Laboratory of MaricultureEpidemiology and BiosecurityYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China

Pyropia yezoensis(formerly Porphyra yezoensis)is an economically important red alga that is cultured extensively in *** red rot disease occurs commonly during Pyropia cultivation,causing serious economic *** incidence of red rot disease was found in a *** farm from mid-November to mid-December 2015 at Lianyungang,Jiangsu Province,*** examination revealed that the naturally infected thalli were infected apparently by a pathogen,leading to red rot *** causative agent was isolated and identified as the oomycete Pythium chondricola by morphological analysis and sequence analysis of the internal transcribed spacer and cytochrome oxidase subunit 1(cox 1).In artifi cial infection experiments on the *** blades,the *** isolate was able to cause the same characteristic histopathology seen in natural *** grew well at a wide range of temperatures in the range 8-31℃,salinities at 0-45 and pH *** an orthogonal test used to determine the effects of environmental factors(temperature,salinity,and zoospore concentration)on infection,the data revealed that temperature was the most important factor to affect red rot disease development,with the optimal conditions for disease expansion being 20℃,35 salinity,and a zoospore concentration of 10^6 zoospores/*** results obtained from the present study prompted us to set up a comprehensive epidemiological study on Pyropia,which will provide support to maintain the healthy development of the Pyropia industry in China.

关键词： Pyropia yezoensis red rot identification Pythium chondricola pathogenicity disease expansion

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Functional nucleic acid-based cell imaging and manipulation

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Science China Chemistry 2021年第11期64卷 1817-1825页

作者： Yan Zhou Yuting Zhuo Ruizi Peng Yutong Zhang Yulin Du Qiang Zhang Yue Sun liping qiu Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangsha410082China NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangsha410000China

Cells are the basic structural and functional units of *** analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological *** in fluorescence microscopy have allowed real-time monitoring of biological events at the molecular ***,the development of highperformance fluorescence probes has become a critical *** nucleic acids(FNAs)are oligonucleotides with special chemical and biological functions,and aptamers with excellent molecular recognition capability are one of the most important *** have attracted extensive attention in the field of live-cell study,owing to intrinsic advantages of simple synthesis,convenient modification,low immunogenicity and high *** review focuses on recent research progress in fluorescence imaging and manipulation of cells using FNAs,particularly aptamers,as the molecular ***,a summary is provided and the related challenges are discussed.

关键词： functional nucleic acid aptamer fluorescence cell imaging biological manipulation

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