检索结果-南通市图书馆

五叶草莓与凤梨草莓种间杂交F_1代的形态学及SSR标记鉴定

在线全文

学校读者我要写书评

暂无评论

西北农业学报 2010年第11期19卷 145-148页

作者：董静张运涛王桂霞金万梅钟传飞王丽娜常琳琳北京市农林科学院林业果树研究所北京100093

运用传统杂交方法,以五叶草莓为母本、凤梨草莓品种‘达赛莱克特’为父本进行远缘杂交,获得了11个F1代株系,利用形态学性状与分子标记相结合进行种间杂种鉴定。对父母本、F1代主要的植物学特征、生物学特性的调查分析结果表明,F1代在叶... 详细信息

运用传统杂交方法,以五叶草莓为母本、凤梨草莓品种‘达赛莱克特’为父本进行远缘杂交,获得了11个F1代株系,利用形态学性状与分子标记相结合进行种间杂种鉴定。对父母本、F1代主要的植物学特征、生物学特性的调查分析结果表明,F1代在叶、花、果实等方面的很多性状不同于父母本,其中大部分介于双亲之间;植株长势明显强于父母本,具有明显的杂种优势。采用草莓属46对SSR引物进行分子鉴定,利用3对引物鉴定出种间杂种。形态学与SSR标记综合鉴定结果表明,全部F1代株系均为五叶草莓与凤梨草莓的种间杂种。

关键词：五叶草莓凤梨草莓 SSR标记形态性状杂种鉴定

在线全文

学校读者我要写书评

暂无评论

Face Painting

空中英语教室(初级版.大家说英语) 2025年第1期 38-39,55页

作者： Campbell chang Ruth Devlin 不详

Have you ever seen a movie or TV show where an actor is transformed into something?Maybe the person became an animal or a creature from outer space.

关键词： Painting something outer

Mass spectrometry imaging for unearthing and validating quality markers in traditional Chinese medicines

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2025年第1期17卷 31-40页

作者： Zhiyun Wang Huajie chang Qian Zhao Wenfeng Gou Yiliang Li Zhengwei Tu Wenbin Hou Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine Institute of Radiation Medicine Peking Union Medical College & Chinese Academy of Medical Sciences Tianjin Nankai District Wangdingdi Hospital Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair Tianjin Nan Kai Hospital

Quality marker(Q-Marker) is an innovative concept and model for quality control of Traditional Chinese medicines(TCMs),which will navigate the new direction of quality development of ***,how to characterize the overall quality attributes of TCMs and their biological effects is still *** view of this key scientific issue,this paper proposes a research method based on mass spectrometry imaging(MSI) technology for the discovery and confirmation of TCMs *** is powerful in investigating the spatial distribution of molecules in a variety of samples,and visualizing the information obtained from *** this basis,combine with the five principles of TCMs Q-Marker validation,i.e.,specificity,transmission and traceability,testability,prescription compatibility,and validity,were applied to confirm the finalized *** will lead the new direction of quality development of TCMs.

关键词： five principles quality marker quality control traditional Chinese medicines

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Rainbow Village

空中英语教室(初级版.大家说英语) 2025年第2期 46-47,56页

作者： Campbell chang Ruth Devlin 不详

In Taichung,there is a small village of 11 houses called Rainbow *** soldier Yung-Fu Huang created *** bought a house there more than 30 years *** 2010 the village was to be torn down.

关键词： Village village houses

MET receptor tyrosine kinase promotes the generation of functional synapses in adult cortical circuits

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第5期20卷 1431-1444页

作者： Yuehua Cui Xiaokuang Ma Jing Wei chang Chen Neha Shakir Hitesch Guirram Zhiyu Dai Trent Anderson Deveroux Ferguson Shenfeng Qiu Basic Medical Sciences University of Arizona College of Medicine-PhoenixPhoenixAZUSA Department of Medicine University of Arizona College of Medicine-PhoenixPhoenixAZUSA

Loss of synapse and functional connectivity in brain circuits is associated with aging and neurodegeneration,however,few molecular mechanisms are known to intrinsically promote synaptogenesis or enhance synapse *** have previously shown that MET receptor tyrosine kinase in the developing cortical circuits promotes dendritic growth and dendritic spine *** investigate whether enhancing MET in adult cortex has synapse regenerating potential,we created a knockin mouse line,in which the human MET gene expression and signaling can be turned on in adult(10–12 months)cortical neurons through doxycycline-containing *** found that similar to the developing brain,turning on MET signaling in the adult cortex activates small GTPases and increases spine density in prefrontal projection *** findings are further corroborated by increased synaptic activity and transient generation of immature silent *** MET signaling resulted in an increasedα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/N-methyl-Daspartate(AMPA/NMDA)receptor current ratio,indicative of enhanced synaptic function and *** data reveal that enhancing MET signaling could be an interventional approach to promote synaptogenesis and preserve functional connectivity in the adult *** findings may have implications for regenerative therapy in aging and neurodegeneration conditions.

关键词： aging circuit connectivity cortical circuits molecular mechanisms neural regeneration neurodegeneration synapses

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li Weiwei Guo Shuolong Yuan Liangwei Xu chang Lin Xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

Palindrome-mediated DNA nanotubes with cell-specific aptamers to improve targeted antitumor effects and reduce toxicity on non-small cell lung cancer

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2025年第2期68卷 454-466页

作者： Cheng Zheng Lanlan Song chang Yu Lingye Zhu Jing Zhang Ning Wang Mengchu Liu Shini Li Liangxing Wang Zhifa Shen Xiaoying Huang Division of Pulmonary Medicine the First Affiliated Hospital of Wenzhou Medical UniversityKey Laboratory of Interdiscipline and Translational MedicineWenzhou 325000China Intervention Department the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Key Laboratory of Laboratory Medicine Ministry of Education of Chinaand Zhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhou 325035China

Chemotherapy is regarded as a widely used and effective treatment strategy for lung cancer,although most conventional chemotherapeutics cause severe toxic side-effects due to their indiscriminate attacks on both cancerous and normal *** nucleic acid nanomaterials are emerging as a promising drug delivery strategy,their clinical applications are limited by rapid degradation by nucleases and difficulties in targeting cancer *** this study,we have developed a Rhein-loaded aptamer-based DNA nanotube(DNT-S6@Rhein)for the targeted and efficient therapy of non-small cell lung *** the palindrome segments,two specified oligonucleotides were hybridized and folded into the well-defined nanotubes(DNT-S6),with the S6 aptamer distributed *** obtained nanotubes exhibited excellent serum stability and targeting ability towards A549 cells due to the firm structure and decoration of the S6 ***,as an antitumor drug and DNA intercalator,can be effectively inserted into the *** drug-loaded nanotubes rapidly disassembled in intracellular environment and then the released Rhein was found to activate cellular apoptotic process and significantly suppress proliferation,migration and invasion of A549 ***,DNT-S6@Rhein could efficiently accumulate in tumor regions,offering compelling therapeutic efficacy and biocompatibility under both in vitro and in vivo *** findings of this study provide a promising strategy for mitigating the inevitable systemic side-effects of chemotherapy and expand the potential application of DNA nanostructure on targeted drug delivery.

关键词： DNA nanotube self-assembly Rhein targeted drug delivery cancer therapy

No-touch recipient hepatectomy in liver transplantation for liver malignancies: A state-of-the-art review

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 39-44页

作者： Sheng-Jun Xu Qiang Wei Xin Hu chang-Biao Li Zhe Yang Shu-Sen Zheng Xiao Xu NHC Key Laboratory of Combined Multi-organ Transplantation Institute of Organ TransplantationZhejiang UniversityHangzhou 310003China School of Clinical Medicine Hangzhou Medical CollegeHangzhou 310059China General Surgery Cancer CenterDepartment of Gastrointestinal and Pancreatic SurgeryZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhou 310014China Department of Hepatobiliary and Pancreatic Surgery Department of Liver TransplantationShulan(Hangzhou)HospitalZhejiang Shuren University School of MedicineHangzhou 310022China Institute of Translational Medicine Zhejiang UniversityHangzhou 310000China

Tumor recurrence, the Gordian knot of liver transplantation for malignancies, may be attributed to many parameters. The technique of the “classical” recipient hepatectomy is believed to be one of the potential reasons to cause tumor evasion because of the possible increase of circulating tumor cells, thus leading to an increased recurrent rate. On this background, the no-touch oncological recipient hepatectomy technique has been developed. A comprehensive review of the development and the key surgical steps of the no-touch recipient hepatectomy is presented. This technique might improve clinical outcomes, especially for those recipients who are at a high risk for tumor recurrence. Multicenter prospective studies should be set up to further validate the prognostic role of this technique in patients with liver cancer treated with liver transplantation.

关键词： Liver transplantation Hepatocellular cancer Tumor recurrence Surgical technique No-touch recipient hepatectomy Vena cave sparing hepatectomy

Dense carbon nanofiber self-supporting electrode fabricated by orientation/compaction strategy for high volumetric lithium storage capacity

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2025年第01期 229-237页

作者： Haihui Liu Chaoqun Chen Qiang Xu Yan Song Xiangwu Zhang chang Ma Tianjin Municipal Key Lab of Advanced Fiber and Energy Storage Technology Tiangong University CAS Key Laboratory of Carbon Materials Institute of Coal Chemistry Chinese Academy of Sciences Fiber and Polymer Science Program Department of Textile Engineering Chemistry and Science Wilson College of Textiles North Carolina State University

Low bulk density greatly restricts the large-scale application of electrospun carbon-based fiber membrane as electrode in energy storage devices. To solve the above challenges, herein an orientation-compaction densification strategy is proposed to enhance the bulk density and volumetric capacity of PAN-based carbon nanofiber membranes as self-supporting electrode used in lithium-ion batteries（LIBs）. Specifically, highly-oriented fibers are achieved by high-speed roller collecting during electrospinning, and compaction densification is conducted by hot-pressing treatment. The effects of collecting speed and hot-pressing pressure on the morphology, conductivity,bulk density, tensile strength, and flexibility of the obtained carbon nanofiber membrane are *** to conventional fiber membranes, of which fibers are disorderly stacked, the oriented fiber membrane is much easier to achieve dense stacking by compaction. The obtained dense carbon nanofiber membrane demonstrates a bulk density of 0.566 g cm-3, and shows a significantly-enhanced volumetric capacity(318.3 mA h cm-3), high-rate performance(86.6 mA h cm-3at 5 A g-1), and satisfactory cycling stability when used as selfsupporting electrode of LIBs.

关键词：