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河南省部分地区奶牛场传染性鼻气管炎感染情况调查

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现代牧业 2017年第1期1卷 20-22页

作者：许文博皇甫和平石冬梅王林康史静柯潘昊纯李聪聪朱志超宋予震王岩河南牧业经济学院河南郑州450046

为了解河南省奶牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitis virus,IBRv)的感染情况,采用SYBR Green I荧光定量PCR方法,对郑州、新乡、焦作等地区采集的397头奶牛鼻腔、阴道棉拭子进行了IBRv DNA检测。结果:总体检出率为7.... 详细信息

为了解河南省奶牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitis virus,IBRv)的感染情况,采用SYBR Green I荧光定量PCR方法,对郑州、新乡、焦作等地区采集的397头奶牛鼻腔、阴道棉拭子进行了IBRv DNA检测。结果:总体检出率为7.1%,检出率最高场可达73.7%;患病牛群中的检测率(6.5%)高于外观健康牛群中的阳性率(5.9%)。由此证实,河南省奶牛场中存在一定的IBRV感染,并且其感染程度与年龄、机体健康状况、牛场饲养管理和卫生情况具有一定的相关性。

关键词：奶牛传染性鼻气管炎

新型减毒HSV-2疫苗IFN-γELISpot检测方法的建立

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湖北工业大学学报 2024年第4期39卷 57-60页

作者：徐一艳何倩蔡林康刘滨磊湖北工业大学生物工程与食品学院湖北武汉430068 武汉滨会生物科技股份有限公司湖北武汉430073

通过比较不同刺激物、细胞培养时间、细胞接种浓度、免疫途径,建立检测新型减毒HSV-2疫苗细胞免疫水平的IFN-γELISpot方法。对C57BL/6小鼠进行减毒HSV-2疫苗免疫,第14天加强免疫,第21天分离小鼠脾脏淋巴细胞,采用IFN-γELISpot检测该... 详细信息

通过比较不同刺激物、细胞培养时间、细胞接种浓度、免疫途径,建立检测新型减毒HSV-2疫苗细胞免疫水平的IFN-γELISpot方法。对C57BL/6小鼠进行减毒HSV-2疫苗免疫,第14天加强免疫,第21天分离小鼠脾脏淋巴细胞,采用IFN-γELISpot检测该疫苗的细胞免疫水平。结果表明:最佳实验孔刺激物为紫外灭活30 min、原滴度为10^(8)CCID_(50)/mL的疫苗全病毒颗粒,最佳细胞培养时间为24~48 h,最佳细胞接种浓度为3×10^(6)cells/mL,最佳免疫途径是皮下注射。实验建立了针对该疫苗的IFN-γELISpot方法,为该疫苗提供了一种检测细胞免疫水平的方法。

关键词：酶联免疫斑点试验细胞免疫干扰素γ

Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue

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Science China(Life Sciences) 2014年第1期57卷 107-116页

作者： WU LiZhen ZHOU linkang CHEN Cheng GONG JingYi XU Li YE Jing LI De LI Peng MOE key laboratory of Bioinformatics and Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University Department of Biology Hong Kong University of Science and Technology Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Feed Research Institute Chinese Academy of Agricultural Sciences Department of Pathology The Fourth Military Medical University Center of Biomedical Analysis Tsinghua University

Excess lipid storage in adipose tissue results in the development of obesity and other metabolic disorders including diabetes,fatty liver and cardiovascular *** lipid droplet(LD)is an important subcellular organelle responsible for lipid *** previously observed that Fsp27,a member of the CIDE family proteins,is localized to LD-contact sites and promotes atypical LD fusion and ***,a close homolog of Fsp27,is expressed at high levels in brown adipose ***,the exact role of Cidea in promoting LD fusion and lipid storage in adipose tissue remains ***,we expressed Cidea in Fsp27-knockdown adipocytes and observed that Cidea has similar activity to Fsp27 in promoting lipid storage and LD fusion and ***,we generated Cidea and Fsp27 double-deficient mice and observed that these animals had drastically reduced adipose tissue mass and a strong lean *** addition,Cidea/Fsp27 double-deficient mice had improved insulin sensitivity and were intolerant to ***,we observed that the brown and white adipose tissues of Cidea/Fsp27double-deficient mice had significantly reduced lipid storage and contained smaller LDs compared to those of Cidea or Fsp27single deficient ***,these data reveal an important role of Cidea in controlling lipid droplet fusion,lipid storage in brown and white adipose tissue,and the development of obesity.

关键词： Cidea Fsp27 brown adipose tissue white adipose tissue lipid droplet fusion

Regional Prediction of Impending Debris Flow Based on Doppler Weather Radar

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Wuhan University Journal of Natural Sciences 2007年第4期12卷 627-632页

作者： JIANG Yuhong WEI Fangqiang ZHANG Jinghong GU linkang DENG Bo LIU Hongjiang Key Laboratory of Mountain Hazards and Surface Process Chinese Academy of Sciences/Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 Sichuan China Graduate University of Chinese Academy of Sciences Beijing 100049 China Hainan Meteorological and Ecological Academy Haikou 570203 Hainan China Liangshan Meteorological Observatory Xichang 615000 Sichuan China

Debris flow prediction is one of the important means to reduce the loss caused by debris flow. This paper built a regional prediction model of impending debris flow based on regional environmental background （including topography, geology, land use, and etc.）, rainfall and debris flow data. A system of regional prediction of impending debris flow was set up on ArcGIS 9.0 platform according to the model. The system used forecast precipitation data of Doppler weather radar and observational precipitation data as its input data. It could provide a prediction about the possibility of debris flow one to three hours before it happened, and was put into use in Liangshan Meteorological Observatory in Sichuan province in the monsoon of 2006.

关键词： Doppler weather radar debris flow impending prediction

Semi-quantitatively Predicting the Residence Time of Three Natural Products on Endothelin Receptor A by Peak Profiling Using the Receptor Functionalized Macroporous Silica Gel as Stationary Phase

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Journal of Analysis and Testing 2023年第1期7卷 40-52页

作者： Ping Li Bowen Shi linkang Li Jiatai Yin Qingqing Yao Tian Yang Xiaomin Huang Xu Ji Chaoni Xiao Qian Li Xinfeng Zhao College of Life Sciences Northwest UniversityXi’an 710069China Xi’an International Medical Center Hospital Xi’an 710100China Engineering Research Center of Tibetan Medicine Detection Technology Ministry of EducationXizang Minzu UniversityXianyang 712082China

Drug-receptor interaction analysis has been broadly adopted as a tool for the evaluation of the drug-like ***,growing evidence has demonstrated that drug e fficacy and safety are highly related to residence time,which equals the reciprocal of the dissociation rate constant(k_(d))of a drug to its target *** endothelin receptor A(ET_(A)R)as a probe,we immobilized the receptor on the surface of macroporous silica gel through a covalent interaction between the epidermal growth factor tyrosine kinase(EGFR)at the C terminal of ET_(A)R and the covalent inhibitor ibrutinib modified on the gel in a one-step *** a ffinity stationary phase was used to semi-quantitatively determine the residence time of natural products on ET_(A)R and evaluate their drug-like *** k_(d)values of three specific ligands(bosentan,macitentan,and ambrisentan)to ET_(A)R were determined by nonlinear chromatography,peak profiling and peak_(d)*** the data determined in free solution of the three methods,peak profiling is considered as the best-fit method for k_(d)***,peak profiling was applied for predicting the residence time of three natural products(ferulic acid,berberine,and palmatine)on ET_(A)*** the longest residence time of 61.11±3.47 s on ET_(A)R,palmatine was evaluated as the most potent compound,which could be developed as a long-acting lead for the *** demonstrate that the high-performance a ffinity chromatography with immobilized ET_(A)R is an alternative for the semi-quantitative measurement of residence time for the drug-like property evaluation of natural products.

关键词： Endothelin receptor A Residence time Natural product Drug-like property

Analysis on steering characteristics of crawler pipeline robot

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High Technology Letters 2023年第1期29卷 60-67页

作者：耿林康 RAO Jinjun LEI Jingtao School of Mechatronic Engineering and Automation Shanghai UniversityShanghai 200444P.R.China Shanghai Key Laboratory of Intelligent Manufacturing and Robotics Shanghai 200444P.R.China

In order to improve the elbow passing performance and different diameter adaptability of pipeline robot,a supported crawler pipeline robot is designed,which adopts screw nut mechanism and hinge four-bar mechanism to adapt to the complex environment such as variable diameter pipeline and *** steering characteristics passing through the elbow are studied,the kinematic of pipeline robot bending steering is established,the geometric constraint(GC)and steering constraint(SC)in the elbow are analyzed,and the steering experiment is *** results show that the robot can pass through the elbow by the SC *** SC model can reduce the motor current and energy consumption when the robot passes through the elbow.

关键词： crawler pipeline robot steering characteristics geometric constraint(GC) steering constraint(SC)

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罕见病病种遴选与优先主题确定方法的思考

罕见病研究 2024年第2期3卷 269-274页

作者：李柯欣陈敬丹张丁丁郭武栋郑佳音李林康赵琨张抒扬中国罕见病联盟北京罕见病诊疗与保障学会北京100022 中国医学科学院北京协和医院临床医学研究所北京100730 中国医学科学院北京协和医院心内科疑难重症及罕见病全国重点实验室、国家罕见病医疗质量控制中心北京100730 山东大学齐鲁医学院公共卫生学院卫生管理与政策研究中心国家卫生健康委员会卫生经济与政策研究重点实验室(山东大学)山东大学健康偏好研究中心济南250012 国家卫生健康委卫生发展研究中心北京100032 清华大学万科公共卫生与健康学院北京100080

本文通过对罕见病病种遴选和优先主题确定全流程的梳理和总结,对罕见病病种的申报和初步审查、病种主题信息的标准化、病种主题优先级遴选的证据梳理及罕见病主题确定和病种遴选的评价方法等多个环节内容进行了深入分析。以期为后续开... 详细信息

本文通过对罕见病病种遴选和优先主题确定全流程的梳理和总结,对罕见病病种的申报和初步审查、病种主题信息的标准化、病种主题优先级遴选的证据梳理及罕见病主题确定和病种遴选的评价方法等多个环节内容进行了深入分析。以期为后续开展罕见病病种遴选工作、提升罕见病病种遴选的公平合理性和科学性,并进一步推动中国罕见病相关领域的研究和决策提供参考和借鉴。

关键词：罕见病病种遴选优先主题多准则决策分析

基于NICS脉冲信号注入法的变压器绕组变形在线监测装置研究

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高电压技术 2015年第7期41卷 2259-2267页

作者：张重远王彦波张林康张欣华北电力大学河北省输变电设备安全防御重点实验室保定071003

电力变压器匝间故障将导致绝缘击穿,甚至造成整个电网事故的发生,为此,应用在线频率响应分析法检测变压器绕组变形。通过安装于变压器套管表面的非侵入式电容传感器（NICS）将测试信号通过高压套管注入变压器绕组从而实现了绕组变形的... 详细信息

电力变压器匝间故障将导致绝缘击穿,甚至造成整个电网事故的发生,为此,应用在线频率响应分析法检测变压器绕组变形。通过安装于变压器套管表面的非侵入式电容传感器（NICS）将测试信号通过高压套管注入变压器绕组从而实现了绕组变形的在线监测。开发了基于脉冲信号注入法的变压器绕组变形在线监测系统。着重阐述了测试信号的选择,在线信号的注入,保护电路的设计。分析了信号源和采样频率对频响特性测试结果的影响。对一台型号为35 k VA/10 k V/400 V的配电变压器进行测试,并与安捷伦频谱网络分析仪4395A的测试结果进行对比发现,在0~5 MHz频率范围内,两条频响曲线的相关系数为0.955 3。结果表明,该系统能在在线状态下准确、快速地获取变压器绕组数10 MHz内的频响特性。监测系统具有较高的灵敏度和重复性,能有效检测变压器绕组变形。

关键词：变压器 NICS 纳秒脉冲发生器绕组变形频率响应分析法在线监测

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核电商业模式的优化

开放导报 2016年第3期 37-41页

作者：岳林康中国广核集团有限公司广东深圳518000

核电在国家安全中具有特殊战略地位,具有安全要求高、投资大、建设周期长、运行稳定可靠和排放少、环境友好、有全行业系统性风险等行业特点。核电商业模式需要考虑项目投资、运营成本、环境要求、竞争环境和产业政策等约束。本文对核... 详细信息

核电在国家安全中具有特殊战略地位,具有安全要求高、投资大、建设周期长、运行稳定可靠和排放少、环境友好、有全行业系统性风险等行业特点。核电商业模式需要考虑项目投资、运营成本、环境要求、竞争环境和产业政策等约束。本文对核电厂、核电集团以及核电产业三个层面进行分析,提出了全面建立和完善可持续发展商业模式的路径建议。

关键词：核电行业特点商业模式