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Synthetic and Systems Biotechnology 2024年第1期9卷 152-158页

作者： Xiaoning Xu lingwen zhang Yue Cui Jian Kong Tingting Guo State Key Laboratory of Microbial Technology Shandong UniversityNo.72 Binhai RoadQingdao266237PR China

Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics,while food-grade genetic tools are limited in those ***,a Zn^(2+)-controlled gene expression(ZICE)system was identified in the genome of *** CGMCC7.179,including a transcriptional regulator sczAst and a promoter region of cation transporter czcD(PczcDst).Specific binding of the SczAst to the palindromic sequences in PczcDst was demonstrated by EMSA analysis,suggesting the regulation role of SczAst on *** evaluate their possibility to control gene expression in vivo,the sczAst-PczcDst was employed to drive the expression of green fluorescence protein(GFP)gene in *** NZ9000 and *** CGMCC7.179,*** of the transformants could express GFP under Zn^(2+)induction,while no fluorescence without Zn^(2+)*** optimal conditions,Zn^(2+)was used at a final concentration of 0.8 mM in *** and 0.16 mM in *** at OD600 close to 0.4,and omitting yeast extract powder in the medium unexpectedly improved GFP expression level by *** the help of the ZICE system,engineered *** and *** strains were constructed to secret cytokine interleukin-10(IL-10)with immunogenicity,and the IL-10 content in the supernatant of the engineered *** was 59.37%of that under the nisin controlled expression *** study provided a tightly controlled expression system by the food-grade inducer Zn^(2+),having potential in development of engineered probiotics.

关键词： Lactococcus lactis Streptococcus thermophilus Food-grade expression system Engineered probiotic

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扎米亚京《我们》中的权力空间

外文研究 2024年第3期12卷 67-71,108页

作者：张凌闻复旦大学

运用列斐伏尔与福柯的空间理论解读扎米亚京的小说《我们》,能够厘清其中权力政治与空间表征的相互作用机理。小说世界中异时空间共时化的宏观时空架构“大一统国”“墙外世界”和“古屋”助推了文本意义的生成。其中的权力空间叙事通... 详细信息

运用列斐伏尔与福柯的空间理论解读扎米亚京的小说《我们》,能够厘清其中权力政治与空间表征的相互作用机理。小说世界中异时空间共时化的宏观时空架构“大一统国”“墙外世界”和“古屋”助推了文本意义的生成。其中的权力空间叙事通过“规训与反抗”一组相对立的权力维度得以展开:大一统国依托禁闭性物理空间设计与单向性精神空间干预对国民实现了深入而有效的理性规训,而小说人物则利用脱离权力作用的多元异质空间采取了积极多样的反抗尝试。

关键词：我们空间理论权力政治规训与反抗

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卡拉乌洛夫的语言个性理论及其核心理念

中国俄语教学 2024年第3期43卷 11-20页

作者：姜宏张凌闻复旦大学

由俄罗斯心理语言学家卡拉乌洛夫建构的语言个性理论不仅在学理内涵和层次结构上具有较大的独创性,而且包孕着许多即便在今天看来仍弥足珍贵的重要理念和思想。就其本质内涵而言,“语言个性”是一个多维度一多层级的聚合体,包括“语言... 详细信息

由俄罗斯心理语言学家卡拉乌洛夫建构的语言个性理论不仅在学理内涵和层次结构上具有较大的独创性,而且包孕着许多即便在今天看来仍弥足珍贵的重要理念和思想。就其本质内涵而言,“语言个性”是一个多维度一多层级的聚合体,包括“语言中的人”与“人说的语言”两个方面,分别呈现在语言掌握、语言认知、语言使用三个层级上,在实际言语活动中主要体现为“言语个性”。在语言个性理论框架内,语言与其主体(人)之间、个体与民族之间、个体与社会之间以及不同语言学范式、不同学科和不同方法之间的界限均被跨越,从而形成了一个跨范式、跨学科、跨方法的综合性研究模式,蕴含着丰富的语言学哲学思想。

关键词：卡拉乌洛夫语言个性信俄语语言个性语言学哲学综合性研究方法

冷等离子体处理的面筋蛋白对鸡肉糜凝胶品质特性的影响

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食品工业科技 2024年第6期45卷 84-90页

作者：韩西平计红芳张莹李丹丹张令文马汉军河南科技学院食品学院河南新乡453003 国家猪肉加工技术研发专业中心河南新乡453003

以鸡肉为原料,向其添加不同功率(350、500、650、800、950 W)冷等离子体处理的面筋蛋白,制得鸡肉糜凝胶,探究不同处理面筋蛋白对鸡肉糜凝胶蒸煮损失、保水性、流变特性、质构、化学作用力、微观结构和水分迁移等品质特性的影响。结果表... 详细信息

以鸡肉为原料,向其添加不同功率(350、500、650、800、950 W)冷等离子体处理的面筋蛋白,制得鸡肉糜凝胶,探究不同处理面筋蛋白对鸡肉糜凝胶蒸煮损失、保水性、流变特性、质构、化学作用力、微观结构和水分迁移等品质特性的影响。结果表明:添加冷等离子体处理的面筋蛋白均可提高凝胶的保水性、降低蒸煮损失,650 W时保水性最高为96.47%、蒸煮损失最低为5.43%,与其它组相比均差异显著(P<0.05);面筋蛋白的添加显著提高了凝胶不易流动水的比例(P<0.05),降低了自由水的比例,T_(21)、T_(22)在0~650 W时向短方向移动,650 W时T_(21)、T_(22)最短。随冷等离子体处理功率的增加,储能模量G′先升高后下降,650 W时最高;硬度与咀嚼性持续上升,而弹性和回复性先上升后下降,二者均在650 W时达到最高,分别为0.954和0.367;添加冷等离子处理的面筋蛋白提高了凝胶的疏水作用和二硫键。冷等离子体处理的面筋蛋白可提高凝胶的形成能力、改善品质,650 W时凝胶的空间网状结构致密有序,品质最佳。

关键词：冷等离子体面筋蛋白功率鸡肉凝胶品质

A Novel UWB Signal Sampling Method for Localization based on Compressive Sensing

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China Communications 2010年第1期7卷 65-72页

作者： zhang lingwen Tan Zhenhui Beijing Jiaotong Univ State Key Lab Rail Traff Control & Safety Beijing 100044 Peoples R China

Ultra-wide-band (UWB) signals are suitable for localization, since their high time resolution can provide precise time of arrival (TOA) estimation. However, one major challenge in UWB signal processing is the requirement of high sampling rate which leads to complicated signal processing and expensive hardware. In this paper, we present a novel UWB signal sampling method called UWB signal sampling via temporal sparsity (USSTS). Its sampling rate is much lower than Nyquist rate. Moreover, it is implemented in one step and no extra processing unit is needed. Simulation results show that USSTS can not recover the signal precisely, but for the use in localization, the accuracy of TOA estimation is the same as that in traditional methods. Therefore, USSTS gives a novel and effective solution for the use of UWB signals in localization.

关键词： localization sampling Ultra-Wide-Band (UWB) signal Compressive Sensing (CS)

Energy-efficiency-aware relay selection in distributed full duplex relay network with massive MIMO

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Science China(Information Sciences) 2017年第2期60卷 126-140页

作者： Yongzhi LI Cheng TAO Liu LIU lingwen zhang Institute of Broadband Wireless Mobile Communications Beijing Jiaotong University National Mobile Communications Research Laboratory Southeast University

This paper considers a distributed full duplex relay network where multiple sources simultaneously transmit their signals to multiple destinations via the cooperation of a set of relay stations(RSs). Each RS is assumed to be equipped with large antenna arrays while all sources and destinations only have a single *** assume the channels are Rician fading and the RSs use linear processing to process the signals with imperfect channel state information(CSI). We derive the closed-form expressions of the end-to-end achievable rates for the maximum-ratio combining/matched-filter(MRC/MF) and the zero-forcing(ZF) processing. These results are then used to pursue a detailed analysis of the power saving of the relay network. Then we study the energy-efficiency-aware relay selection strategy since the energy efficiency affects network lifetime in future wireless network. We propose a sub-optimal strategy with low complexity that only requires the statistical *** show that the energy efficiency of the system can be improved with optimal power allocation and our proposed strategy performs very close to the exhaustive search algorithm which is optimal.

关键词： relay network full duplex massive MIMO relay selection energy efficiency

Improved Eigenstructure-Based 2D DOA Estimation Approaches Based on Nystrom Approximation

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China Communications 2019年第1期16卷 139-147页

作者： lingwen zhang Siliang Wu Guanze Peng Wenkao Yang School of Electronic and Information Engineering Beijing Jiaotong University ECE department Tandon School of EngineeringNYU

In this paper,we propose improved approaches for two-dimensional(2 D) direction-of-arrival(DOA) estimation for a uniform rectangular array(URA).Unlike the conventional eigenstructure-based estimation approaches such as Multiple Signals Classification(MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique(ESPRIT),the proposed approaches estimate signal and noise subspaces with Nystr?m approximation,which only need to calculate two sub-matrices of the whole sample covariance matrix and avoid the need to directly calculate the eigenvalue decomposition(EVD) of the sample covariance ***,the proposed approaches can improve the computational efficiency greatly for large-scale *** results verify the reliability and efficiency of the proposed approaches.

关键词： two-dimensional(2D)direction-of-arrival(DOA)estimation uniform rectangular array(URA) Nystrom approximation

Generalized Physical Layer Channel Model for RelayBased Super Dense Networks

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China Communications 2015年第8期12卷 123-131页

作者： zhang lingwen LIU Chang zhang Jiayi WU Faen Institute of Broadband Wireless Mobile Communications Beijing Jiaotong University Department of Electronic Engineering Tsinghua University School of Electronics and Information Engineering Beijing Jiaotong University

The κ-μ fading model is an advanced channel model in super dense wireless *** this paper,we evaluate the performance of the system over κ-μ fading channel in super dense relay networks with consideration of multiple independent but not necessarily identically distributed(i.n.i.d.) cochannel interference(CCI) under interferencelimited *** specifically,we derive a useful and accurate cumulative distribution function(CDF) expression of the end-to-end signal-to-interference plus noise(SINR) ***,we derive novel analytical expressions of the outage probability(OP),average bit error probability(ABEP) and average capacity for binary modulation types and arbitrary positive values of κ-and μ of such ***,we propose asymptotic analysis for both the OP and ABEP to give physical insights.A simplified analytical form for the ABEP at high-SNR regimes is provided as ***,the accuracy of the derived expressions is well validated by Monte Carlo simulations.

关键词： super dense generalized fadingmodel relay co-channel interference decode-and-forward outage probability average biterror probability average capacity.

Performance of Relay Networks in Fading Environments with Dominant Specular Components

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China Communications 2016年第12期13卷 69-78页

作者： lingwen zhang Chang Liu Jiayi zhang Faen Wu Wenkao Yang School of Electronics and Information Engineering Beijing Jiaotong University School of Information and Communication Engineering Beijing University of Posts and Telecommunications School of Science Beijing Jiaotong University

Radio propagation in dense and super dense wireless networks as well as indoor-to-outdoor picocell networks can have multiple line-of-sight or multiple specular components. The performance of a dual-hop decode-and-forward relaying system over multiple specular components fading channels(MSCC)with multiple Rayleigh distributed co-channel interferers in an interference-limited environment is investigated. The MSCC fading model is designed to allow direct and meaningful comparisons to be made between line-of-sight channels and non-line-of-sight channels, with exact parameter correspondences. Comparisons of outage and bit error performance between Nakagami-m/Rayleigh and MSCC/Rayleigh fading environments show that the MSCC model is needed to describe line-of-sight channels that cannot be accurately modeled by the Nakagami-m, or other fading models.

关键词： Multiple Specular Components Channel(MSCC) cochannel interference decode-and-forward relay outage probability average bit error probability

An Efficient Machine Learning Approach for Indoor Localization

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China Communications 2017年第11期14卷 141-150页

作者： lingwen zhang Yishun Li Yajun Gu Wenkao Yang School of Electronics and Information Engineering Beijing Jiaotong University Beijing 100044 China

Indoor localization has gained much attention over several decades due to enormous applications. However, the accuracy of indoor localization is hard to improve because the signal propagation has small scale effects which leads to inaccurate measurements. In this paper, we propose an efficient learning approach that combines grid search based kernel support vector machine and principle component analysis. The proposed approach applies principle component analysis to reduce high dimensional measurements. Then we design a grid search algorithm to optimize the parameters of kernel support vector machine in order to improve the localization accuracy. Experimental results indicate that the proposed approach reduces the localization error and improves the computational efficiency comparing with K-nearest neighbor, Back Propagation Neural Network and Support Vector Machine based methods.

关键词： indoor localization machine learning SVM PCA