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WheatCENet:A Database for Comparative Co-expression Network Analysis of Allohexaploid Wheat and Its Progenitors

Genomics, Proteomics & Bioinformatics 2023年第2期21卷 324-336页

作者： Zhongqiu Li Yiheng Hu Xuelian Ma lingling Da Jiajie She Yue Liu Xin Yi Yaxin Cao Wenying Xu Yuannian Jiao Zhen su State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBejing 100193China StateKey Laboratory of Systematic and Eolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Bejing 100049China

Genetic and epigenetic changes after polyploidization events could result in variable gene expression and modified regulatory ***,using large-scale transcriptome data,we constructed co-expression networks for diploid,tetraploid,and hexaploid wheat species,and built a platform for comparing co-expression networks of allohexaploid wheat and its progenitors,named *** is a platform for searching and comparing specific functional coexpression networks,as well as identifying the related functions of the genes clustered *** annotations like pathways,gene families,protein-protein interactions,microRNAs(miRNAs),and several lines of epigenome data are integrated into this platform,and Gene Ontology(GO)annotation,gene set enrichment analysis(GSEA),motif identification,and other useful tools are also *** WheatCENet,we found that the network of WHEAT ABERRANT PANICLE ORGANIZATION I(WAPOI)has more co-expressed genes related to spike development in hexaploid wheat than its *** also found a novel motif of CCWWWWWWGG(CArG)specifically in the promoter region of WAPO-Al,suggesting that neofunctionalization of the WAPO-AI gene affects spikelet development in hexaploid *** is useful for investigating co-expression networks and conducting other analyses,and thus facilitates comparative and functional genomic studies in wheat.

关键词： Co-expression network Species comparison Diploid and polyploid wheat Functional annotation

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持续低温天气下设施口感型番茄管理技术

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蔬菜 2024年第5期 81-84页

作者：苏铁吴兴彪胡博郝晓建曹玲玲杨明宇北京市密云区农业技术推广站北京101500 北京市密云区农业服务中心北京101500 北京南山农业生态园有限公司北京101500 北京市农业技术推广站北京100029

设施可局部范围改善或创造出适宜植物生长的环境条件,在一定程度上可有效控制和减轻灾害天气对作物的不良影响,然而,极端灾害天气仍会对设施蔬菜产生危害。为减少冬季低温持续时间长等极端天气对设施蔬菜造成的经济损失,减轻其对蔬菜市... 详细信息

设施可局部范围改善或创造出适宜植物生长的环境条件,在一定程度上可有效控制和减轻灾害天气对作物的不良影响,然而,极端灾害天气仍会对设施蔬菜产生危害。为减少冬季低温持续时间长等极端天气对设施蔬菜造成的经济损失,减轻其对蔬菜市场供应平衡的影响,分析了持续低温对设施口感番茄生产的影响,梳理了设施口感番茄生产中应对持续低温灾害天气的技术措施,如增光增蓄热、强保温减散热、应急增温保秧保果、严格把控棉被放揭、合理开关风口、科学水肥管理、保花保果促熟果、病虫害防控等,为未来更好地应对可能发生的类似灾害天气提供技术支撑,帮助广大生产主体及时止损、减损。

关键词：设施口感型番茄持续低温技术

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Recombinant adenovirus-p53(Gendicine) sensitizes a pancreatic carcinoma cell line to radiation

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Chinese Journal of Cancer Research 2013年第6期25卷 715-721页

作者： Jinluan Li Jianji Pan Xianggao Zhu Ying su lingling Bao sufang Qiu Changyan Zou Yong Cai Junxin Wu Ivan W.K.Tham Department of Radiation Oncology Teaching Hospital of Fujian Medical UniversityFujian Provincial Cancer Hospital Department of Radiation Oncology Peking University People's Hospital Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Radiation OncologyPeking University Cancer Hospital & Institute Department of Radiation Oncology National University Cancer Institute

Objective： In this study, we examine the effects of recombinant adenovirus-p53 （rAd-p53） on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation. Methods： Following transfection of SW1990 cells with rAd-p53, we measured expression of P53, P21 and Bax by immunocytochemistry. Both transfected and control cell lines were irradiated with a range of doses, and the survival fractions （SF） were calculated. Dose survival cttrves were constructed and modeled for comparison. Results： Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53, P21 and Bax in a time-dependent manner. At 96 h after transfection, 89.92% of cells expressed P53, 56.8% expressed P21, and 76.50% expressed Bax. The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells, suggesting that rAd-p53 sensitizes SW1990 cells to radiation （Do for the experimental and control groups was 2.199 and 2.462, respectively）. Conclusions： Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53, and this sensitizes the cell line to irradiation. This work suggests that combining rAd-p53 with radiation therapy in pancreatic cancer may be therapeutically beneficial.

关键词： Pancreatic carcinoma recombinant adenovirus-p5 3 (rAd-p 5 3 ) transfection radiosensitization

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Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2-and CDKNIA-interacting protein （BCCIP） in cells

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Protein & Cell 2016年第10期7卷 749-760页

作者： Jiaming su Yi sui Jian Ding Fuqiang Li Shuang Shen Yang Yang Zeming Lu Fei Wang lingling Cao Xiaoxia Liu Jingji Jin Yong Cai School of Life Sciences Jilin University 2699 Qianjin Street Changchun 130012 China Department of Gynecologic Oncology The First Clinical Hospital JJilin University 71 Xinmin Street Changchun 130021 China National Engineering Laboratory for AIDS Vaccine Jilin University 2699 Qianjin Street Changchun 130012 China Key Laboratory for Molecular Enzymology and Engineering The Ministry of Education Jilin University 2699 Qianjin Street Changchun 130012 China The first Hospital and Institute of Epigenetic Medicine Jilin University Changchun 130061 China

The BCCIP （BRCA2. and CDKNIA-interacting protein） is an important cofactor for BRCA2 in tumor suppression. Although the low expression of BCClP is observed in multiple clinically diagnosed primary tumor tissues such as ovarian cancer, renal cell carcinoma and col- orectal carcinoma, the mechanism of how BCCIP is regulated in cells is still unclear. The human INO80/YY1 chromatin remodeling complex composed of 15 sub- units catalyzes ATP-dependent sliding of nucleosomes along DNA. Here, we first report that BCClP is a novel target gene of the INO80/YY1 complex by presenting a series of experimental evidence. Gene expression studies combined with siRNA knockdown data locked candidate genes including BCCIP of the INO80/YY1 complex. Silencing or over-expressing the subunits of the INO80/YY1 complex regulates the expression level of BCCIP both in mRNA and proteins in cells. Also, the functions of INO80/YY1 complex in regulating the transactivation of BCCIP were confirmed by luciferase reporter assays. Chromatin immunoprecipitation （CHIP） experiments clarify the enrichment of INO80 and YY1 at ＋0.17 kb downstream of the BCClP transcriptional start site. However, this enrichment is significantly inhibited by either knocking down INO80 or YY1, suggesting the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCClP promoter region. Our findings strongly indicate that BCClP is a potential target gene of the INO80/YY1 complex.

关键词： BCCIP human INO80 chromatinremodeling complex

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江苏省农村地区居民食管癌及癌前病变影响因素分析

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中国肿瘤外科杂志 2024年第2期16卷 116-121页

作者：刘付东吴玲玲缪伟刚韩仁强苏健周金意罗鹏飞盐城市疾病预防控制中心慢性病防制科江苏盐城224100 江苏省疾病预防控制中心慢性非传染病防制所江苏南京210009

目的了解江苏省农村地区居民食管癌及癌前病变检出情况及影响因素,为食管癌的预防提供理论依据。方法2017年1月至2022年12月“江苏省农村上消化道癌早诊早治”项目采用整群抽样的方法抽取40~69岁常住居民参与研究,选取其中51198名40~69... 详细信息

目的了解江苏省农村地区居民食管癌及癌前病变检出情况及影响因素,为食管癌的预防提供理论依据。方法2017年1月至2022年12月“江苏省农村上消化道癌早诊早治”项目采用整群抽样的方法抽取40~69岁常住居民参与研究,选取其中51198名40~69岁居民作为调查对象,进行危险因素问卷调查、内镜检查和组织活检,根据病理诊断确定食管癌癌前病变或食管癌。采用SPSS 22.0软件进行χ^(2)检验。采用无序多分类Logistic回归分析食管癌前病变和食管癌的影响因素。结果51198名调查对象检出食管癌前病变1279例,检出率2.50%,食管癌741例,检出率1.45%。无序多分类Logistic回归分析结果显示,高中及以上教育水平(OR=0.81,95%CI:0.66~0.98)、家庭收入≥5万(OR=0.82,95%CI:0.73~0.93)和每周食用1~2次新鲜水果(OR=0.70,95%CI:0.52~0.93)是食管癌前病变低风险相关的因素;男性(OR=1.45,95%CI:1.27~1.65)、年龄(OR=5.35,95%CI:4.22~6.79)、食用腌晒食物(<2次/周:OR=1.80,95%CI:1.42~2.28;≥2次/周:OR=3.01,95%CI:2.35~3.87)、食管炎(OR=1.65,95%CI:1.19~2.31)和吃饭生气(OR=2.43,95%CI:2.07~2.85)是食管癌前病变高风险相关的因素。家庭收入≥5万(OR=0.74,95%CI:0.64~0.86)是食管癌低风险相关的因素;男性(OR=1.64,95%CI:1.38~1.95)、年龄(OR=5.54,95%CI:4.11~7.46)、每周食用1~2次霉变食物(OR=1.87,95%CI:1.38~2.54)、食管炎(OR=1.86,95%CI:1.22~2.86)和吃饭生气(OR=2.95,95%CI:2.37~3.67)是食管癌高风险相关的因素。结论江苏省农村地区居民食管癌及癌前病变检出率较高,应加强男性、高龄、有食管炎史和饮食习惯不良人群的食管癌健康知识宣传,以降低食管癌患病风险。

关键词：食管癌癌前病变危险因素无序多分类logistic回归分析

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Bilateral inferior petrosal sinus sampling for the treatment of Cushing's disease Data from 52 cases from one institute over an eight-year period

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Neural Regeneration Research 2011年第28期6卷 2203-2210页

作者： Changyan Fan Xiuhua Shi Qingfang sun Liuguan Bian Weiguo Zhao Jiankang Shen Hua Zhang Tingwei su Weiqing Wang Xiaoying Li Guang Ning Liang Kong lingling Hu Department of Neurology Wuxi No. 3 People＇s Hospital Wuxi 241043 Jiangsu Province China Department of Neurosurgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Radiology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Endocrine and Metabolism Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

The present study analyzed data from 108 Cushing＇s disease patients, who underwent transsphenoidal surgery in one hospital between January 2003 and April 2010, to investigate the performance value of bilateral inferior petrosal sinus sampling （BIPSS）. Of the 108 patients 52 underwent BIPSS prior to surgery and 56 did not. Results showed that BIPSS accuracy for the diagnosis of Cushing＇s disease was 86.5% （45/52）, and accuracy for adenoma lateralization during surgery was 76.9% （40/52）. In addition, early remission rate was significantly greater compared to patients without BIPSS. Results demonstrated that BIPSS facilitated localization and diagnosis of Cushing＇s disease and helped to predict adenoma lateralization.

关键词： bilateral inferior petrosal sinus Cushing's disease Cushing's syndrome pituitary adenoma transsphenoidal surgery

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ABO亚型分子遗传学分析及罕见等位基因鉴定

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中国输血杂志 2024年第2期37卷 145-150页

作者：宿军王夕钢杨红霞车玲玲任甜甜杨春晴赵玲王胜潍坊市中心血站潍坊市血液研究所山东潍坊261043 潍坊市人民医院输血科

目的研究95例血清学ABO亚型标本的分子机制。方法对95例血清学ABO正反定型不符亚型标本全部进行血清学确认和聚合酶链反应-序列特异性(PCR-SSP)ABO基因分型,对PCR-SSP无法检测到亚型等位基因的标本分别先后进行ABO基因第1~7外显子双链... 详细信息

目的研究95例血清学ABO亚型标本的分子机制。方法对95例血清学ABO正反定型不符亚型标本全部进行血清学确认和聚合酶链反应-序列特异性(PCR-SSP)ABO基因分型,对PCR-SSP无法检测到亚型等位基因的标本分别先后进行ABO基因第1~7外显子双链测序和单链测序,以确定突变位点以及突变点所在基因位置。结果95例标本共检测出34个ABO等位基因。其中5个常见ABO等位基因(ABO*A1.01、ABO*A1.02、ABO*B.01、ABO*O.01.01和ABO*O.01.02)和29个罕见亚型等位基因,包括16个ISBT命名等位基因(ABO*A2.01、ABO*A2.05、ABO*A2.13、ABO*A3.07、ABO*AW.37、ABO*AEL.05、ABO*B3.01、ABO*B3.05、ABO*BW.03、ABO*BW.07、ABO*BW.27、ABO*BEL.03、ABO*cisAB.01、ABO*cisAB.05、ABO*BA.02、ABO*BA.04)和5个dbRBC曾命名等位基因(A223、B309、Bw37、Bel09、Bw40)和8个均未命名新等位基因[ABO*B.01+978C>A、ABO*A1.02+248A>T、ABO*B.01+125dupT、ABO*B.01+(98+1G>A)、ABO*A1.02/ABO*B.01+1A>G,ABO*A1.02/ABO*O.01.01+28G>T,ABO*A1.02/ABO*B.01+538C>T,ABO*A1.02/ABO*O.01.01+797insT,后4个标本因标本量不足无法进行单链测序验证]。95例标本通过PCR-SSP确认亚型等位基因76例(21个ISBT和dbRBC已命名等位基因),剩余19例标本进行ABO基因第1~7外显子测序确认,其中确认8例未命名等位基因标本,剩余11例标本经测序未发现在检测范围内的亚型等位基因。结论研究揭示了95例血清学ABO亚型的分子遗传学背景,发现8种罕见的未命名新等位基因。血清学疑难血型的检测受到患者病理、生理等因素的干扰,ABO亚型鉴定需要血清学检测和基因检测相结合。

关键词：血清学ABO亚型血清学基因测序

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贵州铜仁中寒武统含钾页岩地质特征与钾富集规律研究

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贵州大学学报（自然科学版） 2024年第4期41卷 28-33页

作者：蒋天锐潘文苏特施春华涂灵玲赵爽黄丹周灵高军波贵州省地质矿产勘查开发局一〇三地质大队贵州铜仁554300 贵州大学资源与环境工程学院贵州贵阳550025 贵州大学喀斯特地质资源与环境教育部重点实验室贵州贵阳550025

钾盐是中国生产钾肥的重要战略性矿产资源,中国可用于生产钾肥的可溶性钾矿资源非常短缺,难以保障国家粮食安全生产之需,而难溶性钾矿资源储量丰富,尤其在贵州铜仁地区中寒武统含钾页岩广泛发育。以铜仁地区中寒武统含钾页岩为研究对象... 详细信息

钾盐是中国生产钾肥的重要战略性矿产资源,中国可用于生产钾肥的可溶性钾矿资源非常短缺,难以保障国家粮食安全生产之需,而难溶性钾矿资源储量丰富,尤其在贵州铜仁地区中寒武统含钾页岩广泛发育。以铜仁地区中寒武统含钾页岩为研究对象,对含钾页岩地质特征、钾赋存状态和富集规律进行研究。结果表明:钾富集层主要赋存于寒武系中统敖溪组第二段(∈_(2)a^(2))含炭质粉砂质页岩中,厚度26~36 m,K_(2)O含量7%~14%,平均约9.59%。钾元素主要赋存形式为微斜长石和伊利石,初步认为富钾页岩物源很可能来自陆源长英质含钾长石的风化。微斜长石、伊利石等含钾矿物主要在台地边缘斜坡偏氧化水体中发生沉积,最终形成富钾页岩。

关键词：含钾页岩地质特征富集过程中寒武统贵州铜仁

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Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells

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Bioactive Materials 2022年第5期7卷 32-40页

作者： Haicong Shen Rui su Jiao Peng Lin Zhu Kunyue Deng Qi Niu Yanling Song Liu Yang lingling Wu Zhi Zhu Chaoyong Yang MOE Key Laboratory of Spectrochemical Analysis&Instrumentation Collaborative Innovation Center of Chemistry for Energy MaterialsKey Laboratory for Chemical Biology of Fujian ProvinceState Key Laboratory of Physical Chemistry of Solid SurfacesDepartment of Chemical BiologyCollege of Chemistry and EngineeringXiamen UniversityXiamen361005China Department of Hematology Clinical LaboratoryThe First Affiliated Hospital of Xiamen UniversityXiamen361005China Institute of Molecular Medicine Department of Gastrointestinal SurgeryRenji HospitalSchool of MedicineShanghai Jiao Tong University Shanghai200127China

Circulating tumor cells(CTCs),as important liquid biopsy target,can provide valuable information for cancer progress monitoring and individualized ***,current isolation platforms incapable of balancing capture efficiency,specificity,cell viability,and gentle release have restricted the clinical applications of ***,inspired by the structure and functional merits of natural membrane interfaces,we established an antibody-engineered red blood cell(RBC-Ab)affinity interface on microfluidic chip for high-performance isolation and release of *** lateral fluidity,pliability,and anti-adhesion property of the RBC microfluidic interface enabled efficient CTCs capture(96.5%),high CTCs viability(96.1%),and high CTCs purity(average 4.2-log depletion of leukocytes).More importantly,selective lysis of RBCs by simply changing the salt concentration was utilized to destroy the affinity interface for efficient and gentle release of CTCs without nucleic acid *** this chip,CTCs were successfully detected in colon cancer samples with 90%sensitivity and 100%specificity(20 patients and 10 healthy individuals).After the release process,KRAS gene mutations of CTCs were identified from all the 5 cancer samples,which was consistent with the results of tissue *** expect this RBC interface strategy will inspire further biomimetic interface construction for rare cell analysis.

关键词： Red blood cells Circulating tumor cells Colon cancer Biomimetic interface

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早期两路补液联合大黄芒硝散治疗急性胰腺炎临床观察及作用机制

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中国中医药现代远程教育 2020年第17期18卷 132-134页

作者：黄泽辉林震群周文博施宗明张毅娜张婷婷苏玲玲王珊娜黄国榕陈熹福建省漳州市中医院脾胃病科福建漳州363000

目的研究早期两路补液联合大黄芒硝散治疗急性胰腺炎的临床疗效及作用机制。方法研究采用随机单盲对照法收集60例病例,并根据住院的先后顺序随机分为2组,每组30例。所有患者住院后予常规西药治疗。对照组采用两路补液,一路为常规西药输... 详细信息

目的研究早期两路补液联合大黄芒硝散治疗急性胰腺炎的临床疗效及作用机制。方法研究采用随机单盲对照法收集60例病例,并根据住院的先后顺序随机分为2组,每组30例。所有患者住院后予常规西药治疗。对照组采用两路补液,一路为常规西药输液治疗,另一路为生理盐水补液治疗,补液总量按3 mL/(kg·h)计算,24 h后保留一路常规西药输液治疗。试验组在对照组基础上予大黄芒硝散外敷腹部1周。2组给予相应治疗后观察临床疗效及测定血清CRP、IL-6水平。结果试验组与对照组早期液体复苏达标率分别为96.67%、93.33%,差异无统计学意义(P>0.05);试验组临床疗效优于对照组(P<0.05);试验组CRP下降程度显著优于对照组(P<0.01),且IL-6下降程度优于对照组(P<0.05)。结论早期两路补液联合大黄芒硝散外敷腹部治疗急性胰腺炎疗效显著,其机制可能与降低患者血清CRP、IL-6水平,减轻炎症反应有关。

关键词：两路补液大黄芒硝散急性胰腺炎 C反应蛋白白细胞介素6 腹痛

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