Recombinant adenovirus-p53(Gendicine) sensitizes a pancreatic carcinoma cell line to radiation

作     者：Jinluan Li Jianji Pan Xianggao Zhu Ying Su Lingling Bao Sufang Qiu Changyan Zou Yong Cai Junxin Wu Ivan W.K.Tham 

作者机构：Department of Radiation OncologyTeaching Hospital of Fujian Medical UniversityFujian Provincial Cancer Hospital Department of Radiation OncologyPeking University People's Hospital Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Department of Radiation OncologyPeking University Cancer Hospital & Institute Department of Radiation OncologyNational University Cancer Institute 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2013年第25卷第6期

页      面：715-721页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Fujian Province Natural Science Foundation(No.2012J05139) Beijing Municipal Science & Technology Commission(No.Z111107058811021) 

主　　题：Pancreatic carcinoma recombinant adenovirus-p5 3 (rAd-p 5 3 ) transfection radiosensitization 

摘      要：Objective： In this study, we examine the effects of recombinant adenovirus-p53 （rAd-p53） on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation. Methods： Following transfection of SW1990 cells with rAd-p53, we measured expression of P53, P21 and Bax by immunocytochemistry. Both transfected and control cell lines were irradiated with a range of doses, and the survival fractions （SF） were calculated. Dose survival cttrves were constructed and modeled for comparison. Results： Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53, P21 and Bax in a time-dependent manner. At 96 h after transfection, 89.92% of cells expressed P53, 56.8% expressed P21, and 76.50% expressed Bax. The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells, suggesting that rAd-p53 sensitizes SW1990 cells to radiation （Do for the experimental and control groups was 2.199 and 2.462, respectively）. Conclusions： Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53, and this sensitizes the cell line to irradiation. This work suggests that combining rAd-p53 with radiation therapy in pancreatic cancer may be therapeutically beneficial.