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Critical Role of Cys168 in Noggin Protein's Biological Function

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 181-185页

作者： Wei-Dong LIU Xiang-ling FENG Cai-Ping REN Jian-ling SHI xu-Yu yang Ming ZHAO Liang ZHOU Ke LAN Kai-Tai YAO Cancer Research Institute,Central South University.Changsha 410078,China Cancer Research Institute Central South UniversityChangsha 410078 China

Previous studies have indicated that noggin exerts its neural inducing effect by binding andantagonizing bone morphogenetic protein 4(BMP4).In order to further clarify the relationship between thestructure and the function of noggin,and elucidate the possible mechanism responsible for noggin-BMP4interaction,we generated three noggin mutants,C168S,C174S and C197S,by using a site-directed mu-tagenesis *** expression of wild-type(WT)noggin,C174S or C197S,in Xenopus animal caps(ACs)by mRNA injection converted the explants(prospective ectoderm)into neural tissue,as indicated bythe neural-like morphology and expression of the neural cell adhesion molecule(NCAM)in the ***,ACs expressing C 168S suffered an epidermal fate similar to the control ***,among the threemutants,only C 168S lost the dorsalizing *** studies highlight the critical role played by Cys168in noggin’s biological *** probably participates in the formation of an intermolecular disulfide bridge.

关键词： noggin site-directed mutagenesis neural induction dorsalizing

Effects of marker density and minor allele frequency on genomic prediction for growth traits in Chinese Simmental beef cattle

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Journal of Integrative Agriculture 2017年第4期16卷 911-920页

作者： ZHU Bo ZHANG Jing-jing NIU Hong GUAN Long GUO Peng xu ling-yang CHEN Yan ZHANG Lu-pei GAO Hui-jiang GAO xue LI Jun-ya Laboratory of Molecular Biology and Bovine Breeding Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China

Genomic selection has been demonstrated as a powerful technology to revolutionize animal breeding. However, marker density and minor allele frequency can affect the predictive ability of genomic estimated breeding values （GEBVs）. To investigate the impact of marker density and minor allele frequency on predictive ability, we estimated GEBVs by constructing the different subsets of single nucleotide polymorphisms （SNPs） based on varying markers densities and minor allele frequency （MAF） for average daily gain （ADG）, live weight （LW） and carcass weight （CW） in 1 059 Chinese Simmental beef cattle. Two strategies were proposed for SNP selection to construct different marker densities： 1） select evenly-spaced SNPs （Strategy 1 ）, and 2） select SNPs with large effects estimated from BayesB （Strategy 2）. Furthermore, predictive ability was assessed in terms of the correlation between predicted genomic values and corrected phenotypes from 10-fold cross-validation. Predictive ability for ADG, LW and CW using autosomal SNPs were 0.13＋0.002, 0.21＋0.003 and 0.25＋0.003, respectively. In our study, the predictive ability increased dramatically as more SNPs were included in analysis until 200K for Strategy 1. Under Strategy 2, we found the predictive ability slightly increased when marker densities increased from 5K to 20K, which indicated the predictive ability of 20K （3% of 770K） SNPs with large effects was equal to the predictive ability of using all SNPs. For different MAF bins, we obtained the highest predictive ability for three traits with MAF bin 0.01-0.1. Our result suggested that designing a low-density chip by selecting low frequency markers with large SNP effects sizes should be helpful for commercial application in Chinese Simmental cattle.

关键词： genomic prediction cross-validation Chinese Simmental beef cattle marker density minor allele frequency (MAF)

脓毒性休克患儿血液氧代谢指标变化及其临床意义

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中国当代儿科杂志 2017年第10期19卷 1124-1128页

作者：徐菱阳徐丁兰州大学第二医院儿科甘肃兰州730000

脓毒性休克治疗的关键是提供充足的氧供和改善组织灌注,目前常用的反映氧代谢的指标是乳酸(Lac)和中心静脉血氧饱和度(ScvO_2),但是当乳酸和ScvO_2处于正常范围时,组织仍然可能存在缺氧。静动脉二氧化碳分压差(CO_2 gap)在ScvO_2正常时... 详细信息

脓毒性休克治疗的关键是提供充足的氧供和改善组织灌注,目前常用的反映氧代谢的指标是乳酸(Lac)和中心静脉血氧饱和度(ScvO_2),但是当乳酸和ScvO_2处于正常范围时,组织仍然可能存在缺氧。静动脉二氧化碳分压差(CO_2 gap)在ScvO_2正常时可以准确反映机体氧供情况。本文就乳酸和乳酸清除率、ScvO_2和CO_2 gap在判断组织缺氧时的优点和不足进行综述,以期为脓毒性休克的治疗和病情判断提供依据。

关键词：静动脉二氧化碳分压差中心静脉血氧饱和度氧代谢指标脓毒性休克儿童

Computer-driven automated weaning reduces weaning duration in difficult-to-wean patients

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Chinese Medical Journal 2013年第10期126卷 1814-1818页

作者： LIU ling xu Xiao-ting yang Yi HUANG Ying-zi LIU Song-qiao QIU Hai-bo Department of Critical Care Medicine Nanjing Zhongda HospitalSoutheast University Nanjing Jiangsu 210-009 China

Background Weaning difficulties occur in 31% of total intubated patients, and result in prolonged weaning duration. A computer-driven automated weaning system can perform a spontaneous breathing trial （SBT） automatically and display a message when the trial is successfully passed. Such a system might have a beneficial effect on difficult-to-wean patients. The aim of this study was to examine whether the computer-driven automated weaning system can accelerate discontinuation of mechanical ventilation and improve outcomes in difficult-to-wean patients. Methods This randomized controlled study included 39 difficult-to-wean patients who failed their first spontaneous breathing trial. Before initiating weaning, eligible patients were randomly allocated to wean by computer-driven automated weaning system （CDW group, n=19） or a physician-controlled local protocol （PW group, n=20）. Weaning duration, defined as the time from inclusion until first extubation, was the primary endpoint. Secondary endpoints were total duration of mechanical ventilation, the length of intensive care unit （ICU） stay, the number of reintubations, the mortality rate in the ICU, the number of noninvasive ventilations, the number of complications in the ICU, and the number of ventilator-associated pneumonia cases. Results The weaning duration was reduced with the computer-driven weaning as compared with the usual protocol （median 29.0 hours vs. 45.5 hours, P=0.044）. Total duration of mechanical ventilation and duration of the ICU stay did not differ between the CDW and PW groups. There was no difference in the number of reintubations between the CDW and PW groups （3 and 4 patients, P=0.732）. The study groups showed comparable numbers of tracheostomy, self- extubations, ventilator-associated pneumonia, and non-invasive ventilation. Mortality in the ICU was similar in the CDW and the PW groups （21.1% vs. 20.0%, P=0.935）. Conclusion The computer-driven automated weaning system can reduce weaning duration

关键词： respiration artificial weaning computers airway extubation

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao De-En xu Ming-Lei Wu Ji-Chuan Liu Zi-ling Shi Quan-Hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

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基于小波多尺度分析的X-线头影特征点提取

上海交通大学学报 2001年第9期35卷 1350-1354页

作者：凌旭峰杨杰吕永上海交通大学图像处理与模式识别研究所上海200030

提出了一种基于小波多尺度分析的 X-线头影特征点自动提取方法 .先进行多尺度分解 ,得到不同分辨率的图像 ;接着在低分辨率的图像上处理 ,用 Canny边缘检测算子提取边缘 ;用基于知识的跟踪方法和可变模板法连接边缘 ,得到了骨骼线和软... 详细信息

提出了一种基于小波多尺度分析的 X-线头影特征点自动提取方法 .先进行多尺度分解 ,得到不同分辨率的图像 ;接着在低分辨率的图像上处理 ,用 Canny边缘检测算子提取边缘 ;用基于知识的跟踪方法和可变模板法连接边缘 ,得到了骨骼线和软组织线 ;将轮廓线作为指导信息 ,在高分辨率图像上校正 ,得到高精度的轮廓线 ;根据特征点的几何定义 ,利用轮廓线的位置信息、曲率等属性来提取特征点 .实验表明 ,对于质量较好的 X-线图像 ,该方法具有较高的定位精度。

关键词：小波变换多尺度分析 Canny边缘检测算子 X-线头影测量特征提取图像处理牙科诊断

Bullous pemphigoid associated with acquired hemophilia A: A case report

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World Journal of Clinical Cases 2025年第4期13卷 28-33页

作者： Su-Ye Hu Meng-Can Li Zi-Jia Hao xu-Ya Chai Pei-Sai Li yang Liu Li-Xia Liu Ying xu Pan-Pan yang ling-E Li Department of Dermatology Shijiazhuang Traditional Chinese Medicine HospitalShijiazhuang 050051Hebei ProvinceChina Graduate School Hebei University of Traditional Chinese MedicineShijiazhuang 050051Hebei ProvinceChina Department of Pathology Shijiazhuang Traditional Chinese Medicine HospitalShijiazhuang 050051Hebei ProvinceChina

BACKGROUND Acquired hemophilia A (AHA) is a rare and potentially severe bleeding disordercaused by circulating autoantibodies against factor Ⅷ (FⅧ). In approximately50% of the patients, the condition is associated with autoimmune diseases,cancers, medication use, pregnancy, and the post-partum period. Bullous pemphigoid(BP) is a chronic autoimmune subepidermal blistering disease associatedwith tissue-bound and circulating autoantibodies against BP antigens 180 (BP180)and 230 (BP230). AHA-associated BP has a high mortality rate;hence, the understandingof this disease must *** SUMMARY A 69-year-old man presented with erythema, blisters, blood blisters, and crustsaccompanied by severe pruritus for more than 20 days, and ecchymosis andswelling on his left upper arm for 3 days. Pathological examination revealed asubepidermal blister that contained eosinophils. Laboratory tests showed that theBP180 autoantibody levels had increased, isolated activated partial thromboplastintime was notably prolonged (115.6 s), and coagulation FⅧ activity wasextremely low (< 1.0%). Furthermore, the FⅧ inhibitor titer had greatlyincreased (59.2 Bethesda units). Therefore, the patient was diagnosed as having BP associated with AHA, prescribed 0.05% topical halometasone cream, and transferred to a higher-level hospitalfor effective treatment;however, he died after 2 *** AHA associated BP is rare, dangerous, and has a high mortality rate. Therefore, its timely diagnosis and effectivetreatment are necessary.

关键词： Bullous pemphigoid Acquired hemophilia A Acquired hemophilia A FactorⅧ Case report

A monolithic 3D IGZO-RRAM-SRAM-integrated architecture for robust and efficient compute-in-memory enabling equivalent-ideal device metrics

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Science China(Information Sciences) 2025年

作者： Shengzhe YAN Zhaori CONG Zi WANG Zhuoyu DAI Zeyu GUO Zhihang QIAN xufan LI xu ZHENG Chuanke CHEN Nianduan LU Chunmeng DOU Guanhua yang Xiaoxin xu Di GENG Jinshan YUE lingfei WANG ling LI Ming LIU Institute of Microelectronics of the Chinese Academy of Sciences University of Chinese Academy of Sciences

Abstract Compute-in-memory (CIM) based on various devices such as static random access memory (SRAM) and resistive random access memory (RRAM) and other emerging devices such as indium-gallium-zinc-oxide (IGZO) transistor, magnetoresistive RAM (MRAM), and ferroelectric RAM (FeRAM) has been explored for better performance and energy efficiency on neural networks applications. However, CIM based on a single-type device suffers a variety of non-ideal metrics to reach the simultaneous optimal accuracy, density, and energy efficiency. This work presents equivalent-ideal CIM (Eq-CIM), a monolithic 3D(M3D) IGZO-RRAM-SRAM integrated architecture for robust and efficient compute-in-memory enabling equivalent-ideal device metrics. To overcome the non-ideality (variation, endurance, temperature, etc.) of the single-type devices, system-technology co-optimization (STCO) is performed. This work highlights the non-ideality-aware functionality breakdown for robust high accuracy and simultaneous high density/efficiency, by utilizing 2T0C IGZO for temporal activation storage, RRAM for high-density weight storage, and SRAM for accurate CIM. Device-to-algorithm variation transfer is applied to analyze the system-level accuracy. We benchmark Eq-CIM architecture on CIFAR-10/ImageNet, with 5.06×storage density and 5.05×/2.45×area/energy efficiency compared with single-type-device-based CIM, and high robustness (<0.27%accuracy loss) from-40℃ to 120℃.

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Ni-catalyzed cross-electrophile alkyl-alkyl coupling reactions

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Science China Chemistry 2025年

作者： Li-xu You Lan Tian Chun-ling Guo Shun-Xi Li Yu-Cheng Liu Yu-Long Li Wei Shu College of Chemistry and Environmental Engineering Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan Sichuan University of Science and Engineering Guangming Advanced Research Institute Shenzhen Grubbs Institute Department of Chemistry and Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and Technology

Introducing saturated carbon-carbon bonds into organic molecules significantly increases the three-dimensional configuration,thus altering the physical and chemical properties as well as the functions of molecules. Transition-metal-catalyzed carboncarbon cross-couplings represent one of the most straightforward, efficient and modular alternatives to build saturation in a single step. However, the efficient construction of alkyl-alkyl bonds remains a formidable challenge due to the precise manipulation of multiple alkyl metallic intermediates as well as associated competitive side reactions and selectivity control. Over the past years, Ni-catalyzed cross-coupling under reductive conditions has gained more and more attention for alkyl-alkyl crosscoupling partially attributing to the low-valent state of metal centers under reducing conditions, which suppresses the competitive β-hydride elimination process, thus facilitating the formation of alkyl-alkyl bonds. In this review, the initiative and progress of Ni-catalyzed cross-electrophile alkyl-alkyl cross-coupling, including the efforts in racemic and enantioselective formation of alkyl-alkyl bonds, have been summarized and discussed. Moreover, the challenges and further opportunities in this area have also been discussed.

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