Critical Role of Cys168 in Noggin Protein's Biological Function

作     者：Wei-Dong LIU Xiang-Ling FENG Cai-Ping REN Jian-Ling SHI Xu-Yu YANG Ming ZHAO Liang ZHOU Ke LAN Kai-Tai YAO Cancer Research Institute,Central South University.Changsha 410078,China 

作者机构：CancerResearchInstituteCentralSouthUniversityChangsha410078China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2005年第37卷第3期

页      面：181-185页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：This work was supported by a grant from Joint Research Scheme Project of National Natural Science Foundation of China and the Hong Kong Research Grants Council(No.39910161994) 

主　　题：noggin site-directed mutagenesis neural induction dorsalizing 

摘      要：Previous studies have indicated that noggin exerts its neural inducing effect by binding andantagonizing bone morphogenetic protein 4(BMP4).In order to further clarify the relationship between thestructure and the function of noggin,and elucidate the possible mechanism responsible for noggin-BMP4interaction,we generated three noggin mutants,C168S,C174S and C197S,by using a site-directed mu-tagenesis *** expression of wild-type(WT)noggin,C174S or C197S,in Xenopus animal caps(ACs)by mRNA injection converted the explants(prospective ectoderm)into neural tissue,as indicated bythe neural-like morphology and expression of the neural cell adhesion molecule(NCAM)in the ***,ACs expressing C 168S suffered an epidermal fate similar to the control ***,among the threemutants,only C 168S lost the dorsalizing *** studies highlight the critical role played by Cys168in noggin’s biological *** probably participates in the formation of an intermolecular disulfide bridge.