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Expression of TET and 5-HmC in Trophoblast Villi of Women with Normal Pregnancy and with Early Pregnancy Loss

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Current Medical Science 2018年第3期38卷 505-512页

作者： Ai-hua WU Dong-yu YANG Yu-dong LIU Xin CHEN Xu-long CHEN Shan LU shi-ling CHEN Center for Reproductive Medicine Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou510120 China Center for Reproductive Medicine Department of Gynecology and Obstetrics Nanfang Hospital Southern MedicalUniversity Guangzhou 510515 China

Increasing evidence suggests that epigenetic dysfunction may influence the stability of normal pregnancy. The ten-eleven translocation （TET） family and 5-hydroxymethylcytosine （5-hmC） were found to be linked with epigenetic reprogramming. The present study aimed to examine the expression of the TET family and 5-hmC in the villi of human embryos and compared their expression between normal pregnancy and early pregnancy loss （EPL）. Embryonic villi were collected from normal pregnant women （control） experiencing medical abortion and from EPL patients at gestation ages of 6, 7 and 8 weeks. The mRNAs of TET family were analysed using quantitative polymerase chain reaction （qPCR）, and TET proteins using Western blotting and immunohistochemical analysis. The MethylFlashTM Kit was used to quantify the absolute amount of 5-methylcytosine （5-mC） and 5-hmC. Our results showed that the expression of the TETs and 5-hmC in the normal villus decreased with increasing gestational age. Immunohistochemistry revealed that the TET proteins were expressed in the cytoplasm of trophoblasts and their expression was the highest in the 6-week tissue samples, which was consistent with the qPCR and Western blot results. The expression of TET1, TET2, and TET3 was lower in the villi in EPL group than in normal pregnancy group （P〈0.05 for all）. It was concluded that the TET family and 5-hmC are critical in epigenetic reprogramming of human embryo. The findings also suggest that a deficiency of TETs in the villus might be associated with human EPL.

关键词： early pregnancy loss villus ten-eleven translocation 5-hydroxymethylcytosine 5-methylcytosine

Biodegradable Foaming Material of Poly(butylene adipate-co-terephthalate)(PBAT)/Poly(propylene carbonate)(PPC)

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Chinese Journal of Polymer Science 2022年第2期40卷 208-219页

作者： Han-Lin Tian Ze-Peng Wang shi-ling Jia Hong-Wei Pan Li-Jing Han Jun-Jia Bian Ying Li Hui-Li Yang Hui-Liang Zhang Changchun University of Technology School of Chemical EngineeringChangchun 130012China Key Laboratory of Polymer Ecomaterials Chinese Academy of SciencesChangchun Institute of Applied ChemistryChangchun 130022China Jilin COFCO Biochemistry Packaging Co. Ltd.Liaoyuan 136300China

A biodegradable blend foaming material of poly(butylene adipate-co-terephthalate)(PBAT)/poly(propylene carbonate)(PPC)was successfully prepared by chemical foaming agent and screw extrusion ***,PBAT was modified by bis(tert-butyl dioxy isopropyl)benzene(BIBP)for chain extension,and then the extended PBAT(E-PBAT)was foamed with PPC using a twin(single)screw *** analyzing the properties of the blends,we found that Young’s modulus increased from 58.8 MPa of E-PBAT to 244.7 MPa of E-PBAT/PPC 50/*** viscosity of the polymer has a critical influence on the formation of *** with neat PBAT(N-PBAT),the viscosity of E-PBAT increased by 3396 Pa·s and E-PBAT/PPC 50/50 increased by 8836 Pa·***,the dynamic mechanical analysis(DMA)results showed that the storage modulus(E’)at room temperature increased from 538 MPa to 1650 *** various phase morphologies(“sea-island”,“quasi-co-continuous”and“cocontinuous”)and crystallinity of the blends affected the spread velocity of gas and further affected the foaming morphology in E-PBAT/PPC ***,through the analysis of phase morphology and foaming mechanism,we concluded that the E-PBAT/PPC 70/30 component has both excellent strength and the best foaming performance.

关键词： Poly(butylene adipate-co-terephthalate) Poly(propylene carbonate) Phase structure Biodegradable foam Extrusion foaming

Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9

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Journal of Integrative Agriculture 2015年第7期14卷 1223-1228页

作者： HAN Liu-sha LI Zai-feng WANG Jia-zhen shi ling-zhi ZHU Lin LI Xing LIU Da-qun Syed J A Shah College of Plant Protection Agricultural University of Hebei Nuclear Institute for Food and Agricuture

The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 （resistant）/Zhengzhou 5389 （susceptible） were tested with leaf rust pathotype ＂FHNQ＂ in the greenhouse. Results indicated a 3：1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat （SSR） markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.

关键词： wheat leaf rust resistance gene inheritance analyses molecular mapping

Converting tropical rainforest to native rubber plantations alters soil bacterial and fungal communities

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Circular Agricultural Systems 2022年第1期2卷 48-53页

作者： ling-ling shi De-Li Zhai Hua-fang Chen Centre for Mountain Futures(CMF) Kunming Institute of BotanyKunming 650201YunnanChina Key Laboratory for Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina

Driven by soil biochemistry and plant community composition,soil microbial communities reflect land management and environmental *** evaluate the effects of land-use change on soil microbial diversity,we used denaturing gradient gel electrophoresis(DGGE)combined with sequencing to compare bacterial and fungal community profiles between rubber plantation(RP)and nearby seasonal rainforests(SR).Rainforest soil generally had higher soil total C and microbial biomass C concentration,smaller soil aggregate proportions,and a soil pH below rubber plantation *** bacterial and fungal richness and diversity were similar after converting primary forests to rubber ***,the composition of bacterial and fungal communities has significantly changed in rubber ***,the predominant group of fungi,was significantly different between primary forests and rubber ***,Basidiomycota showed higher heterogenetic distribution in the rainforest under rubber *** conclusion,land-use changes mainly affect soil microbial community composition and heterogeneity distribution patterns,especially for saprotrophic fungi,which consist of changes in litter inputs and soil C conditions.

关键词： soil rubber forest

Wetting Rate and Clay Content Effects on Interrill Erosion in Ultisols of Southeastern China

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Pedosphere 2010年第1期20卷 129-136页

作者： YAN Feng-ling, shi Zhi-Hua , CAI Chong-Fa and LI Zhao-Xia Key Laboratory of Subtropical Agriculture & Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070 (China) Key Laboratory of Subtropical Agriculture & Environment Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 (China)

An aggregate stability test and a simulated rainfall test were conducted on four representative Ultisols from southeastern China. The soils selected, with clay contents ranging between 117 and 580 g kg-1 , were derived from shale and Quaternary red clay. The stability of aggregates (2–5 mm in diameter) obtained from the soil samples were determined by the Le Bissonnais method. For determination of infiltration, runoff, and erosion, the soil samples were packed in 30 cm × 60 cm trays, wetted at rates of 2, 10, and 60 mm h-1 , and then exposed to simulated rainfall at 60 mm h-1 for 1 h. The results indicated that both aggregate stability and slaking caused by fast wetting increased with increasing clay content. The effect of wetting rate (WR) on infiltration and seal formation varied with clay contents. In the soil with low clay content (sandy loam), the infiltration rate was affected slightly by WR due to low aggregate stability and slaking. In the soils with medium clay content (silt clay loam and clay), WR affected infiltration significantly due to the high aggregate slaking force. In the soil with high clay content, the effect of WR on infiltration was significant, but not as evident as in the soils with medium clay content, which may be related to high aggregate stability by wetting partially compensating for slaking force. The effect of WR on soil loss was similar to that of runoff, but more pronounced. The findings from this study indicated that the relationship between wetting rate and clay content should be considered when predicting interrill erosion in Ultisols.

关键词： aggregate stability infiltration seal formation

Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway

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Journal of Integrative Medicine 2022年第2期20卷 163-172页

作者： Hong-xiao Li ling shi Shang-jie Liang Chen-chen Fang Qian-qian Xu Ge Lu Qian Wang Jie Cheng Jie Shen Mei-hong Shen College of Acupuncture Moxibustion and TuinaNanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina Key Laboratory of Acupuncture and Medicine Research of Ministry of Education Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina School of Traditional Chinese Medicine&School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

Objective:Moxibustion,a common therapy in traditional Chinese medicine,has potential benefits for treating decreased ovarian reserve(DOR).The present study investigates the protective effect of moxibustion in a rat model of DOR and explores the possible ***:Sixty-four female Sprague-Dawley rats were randomly divided into four groups:control,DOR,moxibustion(MOX),and hormone replacement therapy(HRT).The DOR rat model was established by intragastric administration of 50 mg/kg Tripterygium glycoside suspension(TGS),once daily for 14 *** and HRT treatments were given from the day TGS administration was *** ovarian reserve function was evaluated by monitoring the estrus cycle,morphological changes in ovaries,levels of serum estradiol(E2),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and anti-Mullerian hormone(AMH),pregnancy rate and embryo ***-deoxynucleotidyl transferase-mediated nickend-labeling staining was used to identify ovarian granulosa cell apoptosis,while the protein and m RNA expressions of Bax,B-cell lymphoma-2(Bcl-2),phosphatidylinositol 3-kinase(PI3 K)and protein kinase B(AKT)in ovarian tissues were examined by immunohistochemistry,Western blot and quantitative reverse transcription-polymerase chain ***:Compared with the DOR group,MOX improved the disordered estrous cycle,promoted follicular growth,reduced the number of atresia follicles,increased the concentrations of serum E2 and AMH,and decreased serum FSH and LH *** importantly,the pregnancy rate and embryo numbers in DOR rats were both upregulated in the MOX treatment group,compared to the untreated DOR ***,we found that the MOX group had reduced apoptosis of ovarian granulosa cells,increased Bcl-2 expression and reduced expression of ***,the PI3 K/AKT signaling pathway was triggered by the moxibustion ***:Moxibustion improved ovarian function and suppressed apoptosis of ovarian gra

关键词： Decreased ovarian reserve Moxibustion Phosphatidylinositol 3-kinase Protein kinase B Apoptosis Infertility

A low-jitter self-triggered spark-discharge pre-ionization switch: primary research on its breakdown characteristics and working mechanisms

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Plasma Science and Technology 2021年第11期23卷 167-176页

作者： Tianchi WANG Yingchao DU Wei CHEN Junna LI Haiyang WANG Tao HUANG Linshen XIE Le CHENG ling shi Key Laboratory of Particle&Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084People's Republic of China Department of Engineering Physics Tsinghua UniversityBeijing 100084People's Republic of China State Key Laboratory of Intense Pulsed Radiation Simulation and Effect Northwest Institute of Nuclear TechnologyXi'an 710024People's Republic of China State Key Laboratory of Electrical Insulation and Power Equipment Xi’an Jiaotong UniversityXi'an 710049People's Republic of China

MV pulsed switch plays a key role as the transfer switch in large electromagnetic pulse simulators. To broaden the range of self-triggering time, a novel spark-discharge pre-ionization switch, in which the main gap electric field is superposed at the trigger gap to let the electrons in its spark channel also become initial electrons, is proposed and tested. The design idea is: as electrons in the spark channel of the trigger gap always exist after its breakdown, the injection time of pre-ionization should have a more negligible effect on reducing the switch jitter. The experiment results under pulses with a rise time of ~100 ns support the above *** the operating voltage is from ~300 to ~800 kV and the self-triggering time is ~45% to~75% of the peak time, the breakdown time delay jitter is less than 2 ns, and the breakdown voltage jitter is smaller than 1.25%. Under specific self-triggering time, the breakdown time delay jitter is less than 1.5 ns, and the breakdown voltage jitter is smaller than 0.8%.

关键词： gas switch self-triggering pre-ionization jitter nanosecond pulse

Superior Toughened Biodegradable Poly(L-lactic acid)-based Blends with Enhanced Melt Strength and Excellent Low-temperature Toughness via In situ Reaction Compatibilization

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Chinese Journal of Polymer Science 2023年第3期41卷 373-385,I0007页

作者： shi-ling Jia Xiang-Yu Wang Ye Zhang Xiang-Yu Yan Hong-Wei Pan Yan Zhao Li-Jing Han Hui-Liang Zhang Li-Song Dong Hui-Xuan Zhang Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Materials Science and Engineering Changchun University of TechnologyChangchun 130012China

The toughened poly(L-lactic acid)/poly(butylene succinate-butylene terephthalate)(PLLA/PBST) blend with enhanced melt strength and excellent low temperature toughness and strength was prepared by melt compounding through in situ compatibilization reaction in presence of multifunctional epoxy compound(ADR).The PLLA/PBST blend was an immiscible system,and the compatibility of the PLLA/PBST blend was improved after adding *** and GPC curves confirmed the formation of the PLLA-g-PBST copolymer,which improved the interfacial bonding of the blend and therefore the PLLA/PBST/ADR blend showed excellent melt strength and mechanical *** the PLLA/PBST/ADR blend with 70/30 PLLA/PBST content,the complex viscosity increased significantly with increasing ADR ***,the tensile strength,elongation at break and impact strength all increased obviously with increasing the ADR *** elongation at break of the blend reached the maximum value of 392.7%,which was 93.2 times that of neat *** the impact strength of the blend reached the maximum value of 74.7 k J/m~2,which was 21.3 times that of neat ***,the PLLA/PBST/ADR blend exhibited excellent lowtemperature toughness and *** –20 ℃,the elongation at break of the PLLA/PBST/ADR blend was as high as 93.2%,and the impact strength reached 18.8 k J/m~***,the tensile strength of the blend at low temperature was also high(64.7 MPa),which was beneficial to the application of PLA in the low temperature *** addition,the PLLA/PBST/ADR blend maintaind good biodegradability,which was of great significance to the wide application of PLLA.

关键词： Poly(L-lactic acid) PBST Compatibilization Super toughness Low-temperature mechanical properties Biodegradability

Diurnal changes in net photosynthetic rate of Pueraria lobataand its impact factors

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Forestry Studies in China 2011年第1期13卷 57-63页

作者： DING You-fang WANG Cai-yun Neo C MOKGOLODI ZHANG Xiao-xia shi ling-ling ZHANG Yun-wei YANG Fu-yu LIU Yu-jun College of Biological Sciences and Biotechnology National Engineering Laboratory for Tree Breeding Beijing Forestry University Beijing 100083 P. R. China College of Animal Sciences and Technology China Agricultural University Beijing 100193 P. R. China

In order to identify the relationship between diurnal changes in the net photosynthetic rate（Pn） of Pueraria lobata and environmental factors,diurnal changes in the Pn of leaves of two *** cultivars were measured using a CIRAS-1 portable photosynthesis measurement system（PP-Systems,UK）.The results show that diurnal changes in Pn of both cultivars could be interpreted as double-peak curves,indicating the occurrence of an obvious midday *** analyses indicate that the correlation between Pn and stomatal conductance was positive and extremely significant（p 〈 0.01）.The correlations of Pn with intercellular CO2 concentration and transpiration rate were positive and significant（p 〈 0.05）,while the correlations of Pn with air and leaf temperatures were negative and significant（p 〈 0.05）.The results indicate that among the factors affecting photosynthetic properties,some can be grouped as stomatal limitations while others are non-stomatal limitations.

关键词： Pueraria lobata net photosynthetic rate (Pn) diumal change environmental factors