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Pig H3K4me3,H3K27ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

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Zoological Research 2024年第1期45卷 138-151页

作者： Tao Jiang Zhi-Min Zhou Zi-Qi ling Qing Zhang Zhong-Zi Wu Jia-Wen Yang Si-Yu Yang Bin Yang Lu-sheng Huang National Key Laboratory of Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely *** serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated ***,we conducted an integrated analysis using H3K27ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult *** aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental *** on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity,results indicated that H3K27ac exhibited stronger associations with gene expression than ***,1.45%of TEs overlapped with either the H3K27ac or H3K4me3 peaks,with the majority displaying tissue-specific ***,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K27ac peaks of the adult and fetal ***-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific *** note,1967 TE-containing transcripts were enriched in the *** identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2(a cell cycle-related protein kinase)in our pig *** element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary ***,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive

关键词： Transposable elements Porcine Histone modification Alternative promoter TE-containing transcript

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基于密度泛函理论与自举软缩减法的酒石酸太赫兹光谱特征谱区分析指认

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光谱学与光谱分析 2022年第9期42卷 2740-2745页

作者：唐鑫周胜灵祝诗平马羚凯郑权普京西南大学工程技术学院重庆402160

太赫兹时域光谱不但包含了样品的化学信息和物理信息,还承载了设备噪声、样品状态、环境参数等多方面的背景信息,其光谱的多元性可能影响模型的性能,降低预测精度。能否在复杂、重叠、变动背景下从光谱数据中提取目标组分的特征信息,去... 详细信息

太赫兹时域光谱不但包含了样品的化学信息和物理信息,还承载了设备噪声、样品状态、环境参数等多方面的背景信息,其光谱的多元性可能影响模型的性能,降低预测精度。能否在复杂、重叠、变动背景下从光谱数据中提取目标组分的特征信息,去除冗余变量,筛选特征谱区,对太赫兹光谱定量、定性分析至关重要。以L-酒石酸为研究对象,在室温下采集6个浓度:10%,20%,40%,50%,60%和80%,共计342个样本的L-酒石酸太赫兹吸收光谱。利用密度泛函理论(DFT)中的B3LYP方法,基于6-31G^(*)(d,p)基组对L-酒石酸单分子模型进行优化并对其太赫兹频谱特性进行理论模拟计算,分析对应特征波峰的分子振动模式,得到0.2~1.6 THz频段吸收谱。与实测吸收谱进行对比,实验所测结果与理论计算结果对应的吸收峰位置基本吻合。采用自举软缩减法(BOSS)对L-酒石酸的太赫兹吸收谱进行特征谱区筛选,并与竞争性自适应加权采样(CARS)、蒙特卡洛无信息变量消除法(MC-UVE)和间隔区间偏最小二乘法(iPLS)3种经典特征谱区筛选法进行对比,分析结果显示BOSS算法选取的有效谱区与DFT理论计算特征谱区重合度最优。分别使用全谱PLS,CARS-PLS,MC-UVE-PLS,iPLS及BOSS五种算法对L-酒石酸光谱进行建模回归分析,实验结果表明,四种谱区筛选方法相较于全谱PLS模型,预测精度均有所提高,其中BOSS算法预测能力提高最为显著,其交互验证均方根误差(RMSECV)、预测均方根误差(RMSEP)、训练集决定系数(R^(2)_(train))和测试集决定系数(R^(2)_(test))分别为0.0260,0.0260,0.9881和0.9875,相较其他模型有更高的预测精度和模型稳定性,为实现基于太赫兹光谱技术的快速定量检测提供了一种有效的方法。

关键词：太赫兹光谱 L-酒石酸密度泛函谱区筛选自举软缩减法

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Chinese Guidelines for the Diagnosis and Management of Atrial Fibrillation

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Journal of Geriatric Cardiology 2024年第3期21卷 251-314页

作者： Chang-sheng MA Shu-Lin WU Shao-Wen LIU Ya-ling HAN Chinese Society of Cardiology Chinese Medical Association Heart Rhythm Committee of Chinese Society of Biomedical Engineering Beijing Anzhen Hospital Capital Medical University Guandong Provincial People’s Hospital Shanghai First People’s Hospital General Hospital of Northern Theater Command 不详

Atrial fibrillation(AF)is the most common sustained cardiac arrhythmia,significantly impacting patients’quality of life and increasing the risk of death,stroke,heart failure,and *** the past two decades,there have been significant breakthroughs in AF risk prediction and screening,stroke prevention,rhythm control,catheter ablation,and integrated *** this period,the scale,quality,and experience of AF management in China have greatly improved,providing a solid foundation for the development of guidelines for the diagnosis and management of *** further promote standardized AF management,and apply new technologies and concepts to clinical practice in a timely and comprehensive manner,the Chinese Society of Cardiology of the Chinese Medical Association and the Heart Rhythm Committee of the Chinese Society of Biomedical Engineering have jointly developed the Chinese Guidelines for the Diagnosis and Management of Atrial *** guidelines have comprehensively elaborated on various aspects of AF management and proposed the CHA2DS2-VASc-60 stroke risk score based on the characteristics of AF in the Asian *** guidelines have also reevaluated the clinical application of AF screening,emphasized the significance of early rhythm control,and highlighted the central role of catheter ablation in rhythm control.

关键词： prevention sustained diagnosis

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基于大数据分析的港口停泊船舶识别方法

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舰船科学技术 2023年第13期45卷 158-161页

作者：凌海生浙江交通职业技术学院海运学院浙江杭州311112

针对港口图像背景复杂、数据量大,影响船舶识别效率的问题,提出基于大数据分析的港口停泊船舶识别方法。选取Hadoop云计算框架,利用分布式处理方法,并行处理海量港口数据。Hadoop利用数据分析平台,运行Top-hat分水岭分割方法,该方法利... 详细信息

针对港口图像背景复杂、数据量大,影响船舶识别效率的问题,提出基于大数据分析的港口停泊船舶识别方法。选取Hadoop云计算框架,利用分布式处理方法,并行处理海量港口数据。Hadoop利用数据分析平台,运行Top-hat分水岭分割方法,该方法利用港口图像的局部极小值确定分水岭位置,计算分水岭位置的梯度函数,依据设置的阈值分割港口图像。将分割后的图像作为卷积神经网络的输入,卷积神经网络通过对图像实施卷积和池化操作,输出港口停泊船舶识别结果。实验结果表明,该方法可以精准识别晴天、雨天等不同背景下的港口停泊船舶,船舶识别时间低于40 ms。

关键词：大数据分析港口停泊船舶识别 Hadoop 分水岭分割卷积神经网络

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Risk factor analysis of post-ERCP cholangitis: A single-center experience

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Hepatobiliary & Pancreatic Diseases International 2018年第1期17卷 55-58页

作者： Min Chen Lei Wang Yun Wang Wei Wei Yu-ling Yao Ting-sheng ling Yong-Hua Shen Xiao-Ping Zou Department of Gastroenterology The Affiliated Drum Tower Hospital of Nanjing University Medical School Department of Gastrointestinal Surgery The Second Affiliated Hospital of Nanjing Medical University

Background: Endoscopic retrograde cholangiopancreatography(ERCP) may have complications. Our study aimed to investigate the risk factors and prevention of post-ERCP ***: We retrospectively analyzed 4234 cases undergone ERCP in the Affiliated Drum Tower Hospital of Nanjing University Medical School from January 2008 to December 2013. Patient-related factors and procedure-related factors were analyzed to find the risk factors of post-ERCP cholangitis. The time point of post-ERCP cholangitis was also analyzed. Univariate and multivariate analyses were performed to define the independent risk factors of post-ERCP ***: The success rate of ERCP was 96.8%(4099/4234). The overall complication rate was 9.4%(399/4234). Post-ERCP cholangitis occurred in 102 cases(2.4%, 102/4234). The most dangerous time of post-ERCP cholangitis was from 24 h–48 h after ERCP(45.1%, 46/102). Univariate analysis revealed that age, hypertension, diabetes, previous ERCP history, biliary stent insertion, pancreatography, endoscopic sphincterotomy, balloon dilation and hilar obstruction were risk factors of post-ERCP cholangitis(P < 0.05). Multivariate analysis indicated that age, previous ERCP history and hilar obstruction were independent risk factors(P < 0.05). While endoscopic stone extraction was the potential protective ***: Many risk factors are involved in post-ERCP cholangitis. Among them, old age, previous ERCP history and hilar obstruction were independently related to this post-ERCP complication.

关键词： Cholangitis Endoscopic retrograde cholangiopancreatography Risk factors Logistic regression analysis

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Synthesis and Crystal Structure of the Binuclear Complex:[Cu_2(phen)_2(ip)(Hip)_2]·4H_2O

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Chinese Journal of Structural Chemistry 2007年第2期26卷 157-160页

作者： SUN Ya-Guang GU Xiao-Fu GAO En-Jun REN ling ZHANG Dong-sheng XU Jie Laboratory of Coordination Chemistry Shenyang Institute of Chemical Technology Shenyang 110142 China

A binuclear complex [Cu2（phen）2（ip）（Hip）2].4H2O has been synthesized by the reaction of Cu（CH3COO）2.H2O, phen（1,10-phenanthroline） and isophthalic acid （H2ip） under hydrothermal condition. Elemental analysis, IR spectra and X-ray single-crystal diffraction were carded out to determine the composition and crystal structure. Crystal data for this complex： monoclinic, space group C2/c, a = 19.214（3）, b = 10.6973（14）, c = 22.567（3） A, β = 105.081（2）°, C24H19N2O8Cu, Mr= 526.95, Z = 8, F（000） = 2160, V= 4478.6（10） A3, Dc = 1.563 g/cm^3,μ = 1.030 mm^-1, -24≤h≤ 16, -13≤k≤13, -28≤l≤28, R = 0.0369 and wR = 0.0819 for 4615 （Rint = 1.0357） independent reflections and 3290 observed ones （I 〉 2σ（I））. Structural analysis shows that coordination geometry of Cu（ Ⅱ ） is a planar square, and the title complex exhibits a 2-D framework supramolecular structure by hydrogen bonds and π-π stacking interactions.

关键词： Cu(Ⅱ) crystal structure isophthalic acid supramolecule

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Study on Triplex DNA by Use of Molecular“hight Switch”Complex of Ru(phen)_(2)(dppx)^(2+)

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Chinese Chemical Letters 2003年第3期14卷 300-303页

作者： Lian sheng ling Zhi Ke HE Fang CHEN Hua Shan ZHANG Yun E ZENG Department of Chemistry Wuhan UniversityWuhan 430072 Institute of Chemistry the Chinese Academy of SciencesBeijing 100080

A new method for the study of triplex DNA is established according the fluorescence enhancement of molecular 揕ight Switch?complex of Ru(phen)2(dppx)2+ when it intercalate into triplex DNA. Because the fluorescence intensity of Ru(phen)2(dppx)2+ bonded to triplex DNA is in the case higher than that bonded to duplex DNA in certain range of DNA concentration, the method is much more sensitive than other methods reported previously.

关键词： Molecular "Light Switch" Ru(phen)2(dppx)2+ Triplex DNA

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基于广义可能性的模态μ演算符号模型检测

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模糊系统与数学 2023年第4期37卷 55-64页

作者：陈娜耿生玲青海师范大学计算机学院青海西宁810008 高原科学与可持续发展研究院青海西宁810008

随着计算机功能的不断增强,软硬件系统也变得越来越复杂,使得人们对系统的需求更高。系统建模中可能存在的诸多不确定性信息也亟待被解决,为了充分解决系统信息来源的种种不确定性特征和各种不完备性,本文引入了广义可能性决策过程理论... 详细信息

随着计算机功能的不断增强,软硬件系统也变得越来越复杂,使得人们对系统的需求更高。系统建模中可能存在的诸多不确定性信息也亟待被解决,为了充分解决系统信息来源的种种不确定性特征和各种不完备性,本文引入了广义可能性决策过程理论作为系统模型。在此基础上,给出了广义可能性模态μ演算的语法和语义;然后通过约简有序二叉决策图进行布尔存储来描述广义可能性不确定信息系统的特征属性;最后给出了广义可能性的模态μ演算符号化模型检测算法和时间复杂度。同时,通过广义可能性的模态μ演算符号化模型检测算法也有效缓解了状态爆炸问题,这对不确定性信息系统的模型检测提供了一种新的思路。

关键词：广义可能性决策过程符号模型检测有序二值决策图模态μ演算

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Diagnosis and treatment of primary pulmonary enteric adenocarcinoma:Report of Six cases

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World Journal of Clinical Cases 2021年第30期9卷 9236-9243页

作者： ling-Fang Tu ling-Yan sheng Jian-Ying Zhou Xue-Fen Wang Yue-Hong Wang Qian Shen Yi-Hong Shen Department of Respiratory Medicine The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina

BACKGROUND Primary pulmonary enteric adenocarcinoma(PEAC)is a very rare subtype of invasive adenocarcinoma,and there have been no large studies on PEAC to ***,it is necessary to obtain much more information about the clinical and pathological features,diagnosis,differential diagnosis,and treatment of *** SUMMARY All clinical data of six patients with confirmed PEAC from 2013 to 2018 were collected,and data on diagnosis,differential diagnosis,and treatment of PEAC are discussed combined with all the associated *** mean age of six patients was 64.0±5.6(59-73)years *** clinical manifestations were heterogeneous,and during their disease course,there were no gastrointestinal *** was no evidence from colonoscopy or imaging studies to suggest digestive tract tumors or new *** most commonly mutated gene was KRAS(50.0%),and the pathological features of the six cases were similar to those of colorectal ***2(83.3%)and CK7(66.7%)had the highest positive rates upon immunohistochemical *** the associated literature,252 cases were identified,and the most commonly mutated gene was KRAS(42.9%).Additionally,CDX2(68.3%)and CK7(85.8%)had the highest positive *** mainly received surgery,chemotherapy,and radiotherapy,immunotherapy was not *** Positive results for CDX2 and CK7 play an important role in the diagnosis and differential diagnosis of PEAC,and immunotherapy or targeted therapy focused on KRAS needs to be further studied for the treatment of PEAC.

关键词： Pulmonary enteric adenocarcinoma Immunohistochemistry Diagnosis Treatment KRAS Case report

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Inhibitory effect of tartary buckwheat seedling extracts and associated flavonoid compounds on the polyphenol oxidase activity in potatoes(Solanum tuberosum L.)

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Journal of Integrative Agriculture 2019年第9期18卷 2173-2182页

作者： LI Jun WANG Hui LU Yang MAO Tang-fen XIONG Jiang HE sheng-ling LIU Hui Guizhou Biotechnology Institute Guizhou Academy of Agricultural SciencesGuiyang 550006P.R.China Food Processing Institute Guizhou Academy of Agricultural Sciences/Potato Engineering Research Center of Guizhou ProvinceGuiyang 550006P.R.China

To improve the processing quality of potatoes,phosphate buffer extract(PBE),50%ethanol(E50),and aqueous extract(AE)of tartary buckwheat seedlings were evaluated for their ability to inhibit the enzymatic browning of *** results suggest that all extracts of tartary buckwheat seedlings exert significant inhibitory effects on the polyphenol oxidase(PPO)activity in *** relative concentrations required for a 50%reduction in the PPO activity(IC50)were 0.21,0.28 and 0.41 mg mL^-1,for PBE,E50 and AE,*** strongest inhibitory activity was observed for PBE,followed by E50 and *** flavone compounds in the PBE of tartary buckwheat seedlings(i.e.,rutin,kaempferol-3-O-rutinoside,quercetin,and kaempferol)were identified by high-performance liquid *** compounds were subsequently evaluated for their roles in the inhibition of PPO from potatoes using a model *** results indicated that rutin exhibited the highest inhibition rate on the PPO of potato.A synergistic inhibitory effect was observed by mixing rutin,kaempferol-3-Orutinoside,quercetin,and *** inhibitory patterns of rutin,kaempferol-3-O-rutinoside,and quercetin on the enzyme were noncompetitive and reversible,with inhibitory constants of 0.12,0.31,and 0.40 mg mL^-1,*** from tartary buckwheat seedlings may exhibit a common mechanism with phenolic compounds,involving the blockage of the reaction of oxygen with PPO leading to the inhibition of the enzymes involved in *** on these results,extracts of tartary buckwheat seedlings can be used as potent natural inhibitors.

关键词： tartary buckwheat natural inhibitor flavonoid compounds polyphenol oxidase potato

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