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Retinoblastoma binding protein 4 represses HIV-1 long terminal repeat-mediated transcription by recruiting NR2F1 and histone deacetylase

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Retinoblastoma binding protein 4 represses HIV-1 long termin...

2019年浙江省检验医学学术大会

作者： juan Wang Yang Zongxing cheng linfang Lu Lingna Pan Kenv Yang Jin Wu Nanping Tongde Hospital of Zhejiang Province The Second Department of Infectious Disease Xixi Hospital of Hangzhou The First Affiliated Hospital College of MedicineZhejiang University Center for Translational Medicine The Affiliated Hospital of Hangzhou Normal University Department of Clinical Laboratory Xixi Hospital of Hangzhou

Human immunodeficiency virus(HIV) transcription is closely associated with chromatin remodeling. Retinoblastoma binding protein 4(RBBP4) is a histone chaperone implicated in chromatin remodeling. However, the role of ...

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Expression of B7-H4 and hepatitis B virus X in hepatitis B virus-related hepatocellular carcinoma

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Expression of B7-H4 and hepatitis B virus X in hepatitis B v...

浙江省免疫学会第十次学术大会

作者： Bo Hong Yun Qian Hong Zhang Yiwen Sang linfang cheng Qi Wang Song Gao Min Zheng Hangping Yao Department of Pathology The Second Affiliated HospitalZhejiang University School of Medicine Department of clinical laboratory The Second Affiliated HospitalZhejiang University School of Medicine Department of Gastroenterology The First Affiliated HospitalZhejiang University School of Medicine State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Institute of Infectious DiseasesThe First Affiliated HospitalZhejiang University School of Medicine

AIM:To investigate the expression and clinical significance of B7-H4 and Hepatitis B virus X(HBx) protein in Hepatitis B virus-related hepatocellular carcinoma(HBV-HCC).METHODS:The expression of B7-H4 in the human HCC cell lines HepG2 and HepG2.2.15 were detected by Western blot,flow cytometry,and *** expression of B7-H4 and HBx in 83 HBV-HCC was detected by immunohistochemistry and the relationship with clinicopathological features was *** section from 83 cases of HBV-HCC patients(22 females and 61 males) were enrolled in this *** age of these patients ranged from 35 to 77 years,with an average of 52.5 ±11.3 *** of these experiments were approved by the Ethics Committees of the Second Affiliated Hospital,Zhejiang University School of ***:B7-H4 was significantly upregulated in HepG2.2.15 cells compared to HepG2 ***,the protein expression levels of B7-H4 in the lysates of HepG2.2.15 cells than in HepG2 *** addition,HBx was expressed only in HepG2.2.15 *** data was confirmed by flow cytometry as similar results were *** positive rates of B7-H4 and HBx in 83 HBV-HCC tissues were 68.67%(57/83) and 59.04%(49/83),*** expression of HBx was also correlated with the TNM *** expression of B7-H4 was positively correlated with HBx(rs= 0.388;P < 0.01).The expression level of B7-H4 in HBx-positive HBV-HCC tissues was substantially higher than that in HBx-negative HBV-HCC *** expression level of B7 H4 was negatively related to the tumor TNM ***:The higher expression of HBx and B7-H4 was correlated with tumor progression of HBV-related hepatocellular ***,B7-H4 may be involved in facilitating HBV-related hepatocarcinogenesis.

关键词： Hepatocellular carcinoma Hepatitis B virus Hepatitis B virus X B7-H4 Immunohistochemistry

Detection of the long non-coding RNAs NEAT1 and MALAT1 in the peripheral blood of HIV-1 infected patients

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Detection of the long non-coding RNAs NEAT1 and MALAT1 in th...

中华医学会第七次全国艾滋病、丙型肝炎学术会议

作者： Jin Changzhong Peng Xiaorong Xie Tiansheng Lu Xiangyun Liu Fumin Wu Haibo Yang Zongxing Wang Juan cheng linfang Wu Nanping State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalSchool of MedicineZhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Objectives:The Long non-coding RNAs(lncRNAs)in HIV-1 infection are not well *** we detected two lncRNAs,NEAT1 and MALAT1,in peripheral blood mononuclear cells(PBMC)and plasma of HIV-1 infected ***:Fifty nine HIV-1 infected patients and 21 healthy controls were recruited,of whom 31 patients were HAART-na?ve and 28 patients received HAART for more

关键词： HIV HAART Detection of the long non-coding RNAs NEAT1 and MALAT1 in the peripheral blood of HIV-1 infected patients

Analysis of the immunogenicity and bioactivities of a split influenza A/H7N9 vaccine mixed with MF59 adjuvant in Balb/c mice

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Analysis of the immunogenicity and bioactivities of a split ...

浙江省免疫学会第十次学术大会

作者： Huilin Ou Wei Yao Nanping Wu Xiaoxin Wu chengcong Han linfang cheng Keda Chen Honglin Chen Hangping Yao Lanjuan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang University

Background:The H7 N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 and stimulated vaccine development *** previous H7-based vaccines have been poorly immunogenic in humans,we sought to determine the immunogenic,protective properties and mechanisms of a split avian influenza A/H7 N9 vaccine mixed with MF59 adjuvant with comparison of other ***:Recombinant H7 N9 influenza seed that possess the hemagglutinin(HA)and neuraminidase(NA) gene segments from the A/Zhejiang/DTID-ZJU01/2013(H7 N9) and six internal protein gene segments from A/Puerto Rico/8/34(H1 N1)(PR8) were generated by reverse *** vaccinated Balb/c mouse with a two doses of different amounts and combinations of this new A/ZJU01/PR8/2013 split vaccine and adjuvant and subsequently challenged with A/Zhejiang/DTID-ZJU01/2013(H7 N9) by intranasal inoculation,the humoral-mediated immune responses including hemagglutination inhibition(HI),Micro-neutralization(MN),IgG antibody *** cell-mediated immune responses including NK assay,CTL assay,cytokine production *** the protection effects against H7 N9 virus were ***:Adjuvants help to increase the immune response to vaccines,achieving higher antibody titers that lead to protection with less amounts of antigen with an acceptable safety ***59 enhanced the HI,MN,IgG antibody titers to influenza antigens at least 2-6 fold higher than the same dose of this H7 N9 split vaccine alone after second immunization and virus incubation,0.05 ml MF59 even boost the response to the HA antigen concentration of 0.024μg which was ineffective alone to achieve protective ***59 has more advantages in inducing the humoral immune response and TH-2 cytokine production after virus attack compared with alum,both the MF59 and alum can increase the NK activity,CD4+ and CD8+subpopulations of splenocytes with no statistical differences between each *** there i

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高速铁路CTCS3+ATO系统优化方案研究

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铁道运输与经济 2021年第4期43卷 80-85页

作者：何志彬程林芳易海旺汪洋中国铁道科学研究院研究生部北京100081 中国铁道科学研究院集团有限公司通信信号研究所北京100081

随着我国高速铁路网逐年扩大,对于自动驾驶的需求不断增加,为满足高速铁路高速度、高密度跨线运营需求,实现装备智能化,阐述高速铁路CTCS3+ATO系统结构及设备功能,分析高速铁路CTCS3+ATO系统需求,考虑到城际铁路CTCS2+ATO系统及高速铁路... 详细信息

随着我国高速铁路网逐年扩大,对于自动驾驶的需求不断增加,为满足高速铁路高速度、高密度跨线运营需求,实现装备智能化,阐述高速铁路CTCS3+ATO系统结构及设备功能,分析高速铁路CTCS3+ATO系统需求,考虑到城际铁路CTCS2+ATO系统及高速铁路CTCS3+ATO系统实际运用情况,结合CTCS3+ATO系统技术特点,提出升级列车定位技术、改变区间闭塞制式、立即折返作业自动化、实现全自动无人驾驶等高速铁路CTCS3+ATO系统优化方案。该方案的实施及应用有助于在保障列车运行安全的前提下,提高自动化运行程度及运输效率。

关键词：高速铁路 CTCS3+ATO 列车自动驾驶系统优化自动驾驶等级

Human cellular miR-1224-3p and miR-1913 seed family microRNAs target on HIV-1 and inhibit virus production

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Human cellular miR-1224-3p and miR-1913 seed family microRNA...

中华医学会第七次全国艾滋病、丙型肝炎学术会议

作者： Lu Xiangyun Yang Jin Jin Changzhong cheng linfang Wu Haibo Wang Juan Yang Zongxing Liu Fumin Peng Xiaorong Xie Tiansheng Yao Hangping Wu Nanping State Key Laboratory for Diagnosis and Treatment of Infectious Disease Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalCollege of MedicineZhejiang University

Objective:Increasing evidences indicate that both cellular and viral derived miRNAs are important players of host-virus *** decide to search for novel cellular miRNAs that can directly target on the HIV-1 transcripts and function to repress HIV-1 ***:The terminal 1.9kb of the 3-UTR of HIV-1 YU2 strain and subfragments were cloned to luciferase and GFP-based miRNA re-

关键词： HIV miRNAs miR microRNAs Human cellular miR-1224-3p and miR-1913 seed family microRNAs target on HIV-1 and inhibit virus production

Preclinical evaluation of the safety and pathogenicity of a live attenuated recombinant influenza A/H7N9 seed strain and corresponding MF59-adjuvanted split vaccine

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Preclinical evaluation of the safety and pathogenicity of a ...

浙江省免疫学会第十次学术大会

作者： Huilin Ou Wei Yao Nanping Wu Frederick X.C.Wang chengcong Han Xiangyun Lu Dongshan Yu Haibo Wu linfang cheng Honglin Chen Hangping Yao Lanjuan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang University

In early spring 2013,human infections with a novel avian influenza A(H7 N9) virus in Eastern China raised concerns of a new influenza *** address these concerns,the development of a safe and effective H7 N9 influenza vaccine is urgently *** this end,we generated candidate H7 N9 vaccine strains using reverse genetics,with the hemagglutinin and neuraminidase genes derived from a human H7 N9 virus and the remaining genes from the PR8 virus,which grows well in ***,our research was conducted to evaluate recombinant clones in BALB/c mice and ferrets to determine whether the recombinants displayed the characteristics desirable in a vaccine strain,including lower virulence and *** established that the virulence and transmissibility of the recombinant H7 N9 vaccine seed strain were decreased as compared to wild-type H7 N9 virus,to levels comparable with *** the seed virus,we produced a monovalent split influenza A(H7 N9) MF59-adjuvanted vaccine that was immunogenic in *** assess the safety of our H7 N9 vaccine,we performed acute toxicity,repeated dose toxicity and active systemic anaphylaxis ***,the vaccine candidate showed no toxicological findings of significance at levels greatly in excess of those proposed for clinical *** conclusion,the comprehensive preclinical evaluation program conducted in the course of the development of an intramuscular MF59-adjuvanted split influenza vaccine documented the safety and immunogenicity of the recombinant H7 N9 vaccine virus strain and safety of the vaccine in animal *** human testing of the MF59-adjuvanted H7 N9 split vaccine will be needed to determine whether it could represent a candidate vaccine in a clinical context

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CTC通信服务器子系统的协议设计

铁道通信信号 2005年第4期41卷 44-46页

作者：程林芳赵随海铁道科学研究院通信信号研究所北京硕士研究生100081

对CTC系统中通信服务器子系统的协议设计进行研究。对于一个数据通信系统而言, 通信的双方是紧密关连的, 因此, 在做系统设计时将通信服务器的设计和各客户端通信部分的设计放在一起, 统一作为系统通信部分来设计。

关键词：通信服务器协议设计子系统数据通信系统 CTC系统系统通信客户端计时

miR-155调节淋巴细胞免疫功能以及与病毒感染的相关性

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国际流行病学传染病学杂志 2016年第1期43卷 47-50页

作者：程林芳吴南屏浙江大学医学院附属第一医院传染病诊治国家重点实验室感染性疾病诊治协同创新中心杭州310003

miR-155在活化淋巴细胞内高表达，参与各种淋巴细胞亚群，包括B细胞、CD8^＋T及CD4^＋T细胞，辅助T细胞1型（Th1）、Th2、Th17和调节性T细胞（Treg）的免疫调节过程。此文就miR-155在分子水平和细胞水平参与淋巴细胞发展、介导调节免疫... 详细信息

miR-155在活化淋巴细胞内高表达，参与各种淋巴细胞亚群，包括B细胞、CD8^＋T及CD4^＋T细胞，辅助T细胞1型（Th1）、Th2、Th17和调节性T细胞（Treg）的免疫调节过程。此文就miR-155在分子水平和细胞水平参与淋巴细胞发展、介导调节免疫应答，以及在单核细胞增生李斯特菌、HIV和HBV等病毒感染中的作用进行综述。

关键词：微RNAs miR-155 淋巴细胞亚群免疫功能病毒感染