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Genetic evidence of Middle east Respiratory Syndrome Coronavirus(MeRS-Cov) and Widespread Seroprevalence among Camels in Kenya

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Virologica Sinica 2018年第6期33卷 484-492页

作者： Sheila Ommeh Wei Zhang Ali Zohaib Jing Chen Huajun Zhang Ben Hu Xing-Yi Ge Xing-Lou Yang Moses Masika Vincent Obanda Yun Luo Shan Li Cecilia Waruhiu Bei Li Yan Zhu Desterio Ouma Vincent Odendo lin-Fa Wang Danielle e.Anderson Jacqueline Lichoti erick Mungube Francis Gakuya Peng Zhou Kisa-Juma Ngeiywa Bing Yan Bernard Agwanda Zheng-Li Shi institute for Biotechnology Research Jomo Kenyatta University of Agriculture and TechnologyNairobi 62000-00200Kenya CAS Key Laboratory for Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China College of Biology Hunan University410006China Department of Medical Microbiology University of NairobiNairobi 30197-00100Kenya Veterinary Services Department Kenya Wildlife ServiceNairobi 40241-00100Kenya Veterinary Research institute Kenya Agriculture and Livestock Research OrganizationNairobi 57811-00200Kenya Veterinary Services Programme in emerging infectious Diseases Duke-NUS Medical SchoolSingapore 169857Singapore Directorate of Veterinary Services State Department of LivestockMinistry of AgricultureLivestock Fisheries and IrrigationNairobi 34188-00100Kenya Kenya Camel Association Nairobi 30095-00100Kenya Department of Zoology National Museums of KenyaNairobi 40658-00100Kenya

We describe the first genome isolation of Middle east respiratory syndrome coronavirus(MeRS-CoV) in Kenya. This fatal zoonotic pathogen was first described in the Kingdom of Saudi Arabia in 2012. epidemiological and molecular evidence revealed zoonotic transmission from camels to humans and between humans. Currently, MeRS-CoV is classified by the WHO as having high pandemic potential requiring greater surveillance. Previous studies of MeRS-CoV in Kenya mainly focused on site-specific and archived camel and human serum samples for antibodies. We conducted active nationwide cross-sectional surveillance of camels and humans in Kenya, targeting both nasal swabs and plasma samples from 1,163 camels and 486 humans collected from January 2016 to June 2018. A total of 792 camel plasma samples were positive by eLiSA. Seroprevalence increased with age, and the highest prevalence was observed in adult camels(82.37%, 95%confidence interval(Ci) 79.50–84.91). More female camels were significantly seropositive(74.28%, 95% Ci 71.14–77.19)than male camels(P \ 0.001)(53.74%, 95% Ci 48.48–58.90). Only 11 camel nasal swabs were positive for MeRS-CoV by reverse transcription-quantitative PCR. Phylogenetic analysis of whole genome sequences showed that Kenyan MeRSCoV clustered within sub-clade C2, which is associated with the African clade, but did not contain signature deletions of orf4 b in African viruses. None of the human plasma screened contained neutralizing antibodies against MeRS-CoV. This study confirms the geographically widespread occurrence of MeRS-CoV in Kenyan camels. Further one-health surveillance approaches in camels, wildlife, and human populations are needed.

关键词： Middle east respiratory syndrome coronavirus(MeRS-CoV) One-health Public health Zoonosis Kenya

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激光熔覆FeCoCrNiNb高熵合金涂层组织及耐磨性能

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稀有金属材料与工程 2023年第4期52卷 1483-1489页

作者：王石王琳尹晓伟沈阳工程学院机械学院辽宁沈阳110870

采用激光熔覆技术在304不锈钢表面制备了FeCoCrNi、FeCoCrNiNb等摩尔比高熵合金熔覆层,研究了Nb元素对熔覆层组织及性能的影响。采用XRD、SeM、eDS、纳米压痕测试和干滑动摩擦磨损实验等方法,详细分析了2种高熵合金熔覆层的相组成、组... 详细信息

采用激光熔覆技术在304不锈钢表面制备了FeCoCrNi、FeCoCrNiNb等摩尔比高熵合金熔覆层,研究了Nb元素对熔覆层组织及性能的影响。采用XRD、SeM、eDS、纳米压痕测试和干滑动摩擦磨损实验等方法,详细分析了2种高熵合金熔覆层的相组成、组织演变、纳米硬度及耐磨性能。结果表明:FeCoCrNiNb高熵合金熔覆层相组成为fcc固溶体及富Nb-Laves相。FeCoCrNiNb熔覆层的纳米硬度(H)、弹性模量(e)、H/e和H^(3)/e^(2)分别为6.066 GPa、231.54 GPa、0.0262和0.0042,远高于FeCoCr Ni熔覆层的3.456 GPa、209.48 GPa、0.0165和0.00094。随着纳米硬度的增加,FeCoCrNiNb熔覆层的摩擦系数和比磨损率也随之降低,分别为0.519和2.54×10^(-6)mm^(3)/(N·m)。综上所述,FeCoCrNiNb高熵合金熔覆层具有良好的纳米硬度和耐磨性。

关键词：高熵合金激光熔覆 Laves相纳米压痕耐磨性

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Haplotype-resolved and chromosome-level genome assembly of Colorado potato beetle

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Journal of Genetics and Genomics 2023年第7期50卷 532-535页

作者： Ziqi Ye Ruirui Lu Chao Li Doudou Yang Zhuozhen Zeng Weichao lin Jie Cheng Zhongmin Yang Li Wang Yulin Gao Sanwen Huang Xingtan Zhang Suhua Li Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsChinese Academy of Agricultural SciencesShenzhenGuangdong 518000China Key Laboratory of Prevention and Control of invasive Alien Species in Agriculture&Forestry of the North-western Desert Oasis Ministry of Agriculture and Rural AffairsCollege of AgronomyXinjiang Agricultural UniversityUrumqiXinjiang Uygur Autonomous Region 830052China School of Life Sciences Henan UniversityKaifengHenan 475004China Shenzhen Research institute of Henan University ShenzhenGuangdong 518000China College of Horticulture Xinjiang Agricultural UniversityUrumuqiXinjiang Uygur Autonomous Region 830052China State Key Laboratory for Biology of Plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Chinese Academy of Tropical Agricultural Sciences HaikouHainan 571101China

As the most important non-cereal food crop,potatoes are a staple food for 1.3 billion people(Stokstad,2019).However,these cultivated potatoes are challenged with multiple biotic stresses(i.e.,pathogens and pests),some of which can evade host resistance,leading to a serious dampening of potato *** of these pests is the Colorado potato beetle(hereafter referred to as CPB,Leptinotarsa decemlineata Say).CPB is a member of the order Coleoptera,and originated in the southwestern United States and Mexico(Alyokhin et al.,2008).

关键词： originated hereafter

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图的匹配设计的矩阵构造法

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系统科学与数学 2000年第2期20卷 140-148页

作者：林翠琴清华大学教学科学系北京100084

完全图Ｋ_ｎ（完全二部图Ｋ_ｎ，ｎ）的一个ｋ－匹配的集合Ｍ，若满足：Ｋ_ｎ（Ｋ_ｎ，ｎ）的每一对独立边恰出现在Ｍ的λ个元素中，则称Ｍ为一个匹配设计，记为ＭＡＴＣＨ（ｎ，ｋ，λ）（ＢＩＭＡＴＣＨ（ｎ；ｋ，λ））一设计．本文... 详细信息

完全图Ｋ_ｎ（完全二部图Ｋ_ｎ，ｎ）的一个ｋ－匹配的集合Ｍ，若满足：Ｋ_ｎ（Ｋ_ｎ，ｎ）的每一对独立边恰出现在Ｍ的λ个元素中，则称Ｍ为一个匹配设计，记为ＭＡＴＣＨ（ｎ，ｋ，λ）（ＢＩＭＡＴＣＨ（ｎ；ｋ，λ））一设计．本文定义两个匹配设计对应的矩阵，并以此构造出某些新的匹配设计．

关键词：完全图完全二部图匹配设计矩阵构造法

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多因共振下的校园贷整治难题

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财经理论与实践 2019年第6期40卷 154-160页

作者：林立成浙江大学光华法学院浙江杭州310008

作为规制难题的非法校园贷,其生成原因是多元的。小额贷款公司与P2P网贷平台长期面临的“融资贵”问题致使其运营成本上升,直接造成二者在利息设定上普遍偏高,容易向高利贷转化。同时,贯穿整个经营过程中的高度不确定的法律风险,使得小... 详细信息

作为规制难题的非法校园贷,其生成原因是多元的。小额贷款公司与P2P网贷平台长期面临的“融资贵”问题致使其运营成本上升,直接造成二者在利息设定上普遍偏高,容易向高利贷转化。同时,贯穿整个经营过程中的高度不确定的法律风险,使得小贷公司与网贷平台更为担忧坏账的发生,当举债学生无法如期还款时,倾向于采用非法催收方式逼迫还债。最后,软暴力手段淡化非法催收的违法性,使非法校园贷的发生更富隐蔽性,加剧了公安机关的介入难度。校园贷监管当在扩大银行、大电商这类正规经营主体在校园信贷市场覆盖面的基础上,不断挤压非法校园贷的生存空间,以期实现良币驱逐劣币的效果。

关键词：非法校园贷融资困境经营风险非法催收警察权

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Brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses

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Journal of innovative Optical Health Sciences 2023年第6期16卷 13-21页

作者： Nengsong Luo Zengpeng Han Jiaxin Kou Yuxiang Cai Xin Yang Jie Wang Kunzhang lin Fuqiang Xu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074P.R.China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430071P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China Shenzhen Key Laboratory of Viral Vectors for Biomedicine Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Productsthe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen518055P.R.China Department of Pathophysiology Key Lab of Neurological Disorder of Education MinistrySchool of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R.China Shenzhen-Hong Kong institute of Brain Science-Shenzhen Fundamental Research institutions Shenzhen518055P.R.China Center for excellence in Brain Science and intelligence Technology Chinese Academy of SciencesShanghai200031P.R.China

Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates,making them powerful tools for determining the structure and function of the complicated neural circuits of the ***,they have some limitations,such as posing high risks to human health and the inability to retrograde trans-synaptic label inputs from genetically-de¯ned starter ***,we established a new retrograde trans-multi-synaptic tracing method through brain-wide rabies virus glycoprotein(RVG)compensation,followed by glycoprotein-deleted rabies virus(RV-△G)infection in specific brain ***,in combination with the avian tumor virus receptor A(TVA)controlled by a cell-type-specific promoter,we found that envA-pseudotyped RV-△G can mediate e±cient retrograde trans-multi-synaptic transduction from cell-type-specific starter *** study provides new alternative methods for neuroscience researchers to analyze the input neural networks of rodents and nonhuman primates.

关键词： Rabies virus retrograde tracers trans-multi-synaptic tracing AAV-PHP.eB celltype-specific input neural networks

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中国近1000年旱涝的持续性特征研究

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物理学报 2008年第6期57卷 3920-3931页

作者：龚志强封国林扬州大学物理科学与技术学院扬州225002 中国科学院大气物理研究所东亚区域气候-环境重点实验室北京100029 国家气候中心中国气象局气候研究开放实验室北京100081

应用中国近531年旱涝指数和近1041年干湿指数,定义干旱(湿润)等级,滑动计算原指数序列各干旱(湿润)等级的出现次数,发现各等级出现的次数与其窗口长度之间均遵循指数分布Pi(x)=Ae-γx.结合指数分布的数理意义,定义指数特征值γ的倒数λ... 详细信息

应用中国近531年旱涝指数和近1041年干湿指数,定义干旱(湿润)等级,滑动计算原指数序列各干旱(湿润)等级的出现次数,发现各等级出现的次数与其窗口长度之间均遵循指数分布Pi(x)=Ae-γx.结合指数分布的数理意义,定义指数特征值γ的倒数λ为描述旱(涝)持续性的尺度因子并对华北和江淮流域旱(涝)发生的持续性特征进行研究.结果表明:旱尺度因子的空间分布表现为由北向南呈带状式波动分布,我国北方地区干旱的持续性相对长江流域要长一些,由北向南三个区的旱尺度因子的均值分别为1·87,1·62,1·82.旱涝指数序列中旱或偏旱(涝或偏涝)相对集中的时段对旱(涝)持续性影响较显著,12世纪末期、13世纪早期、17世纪早期和20世纪末期华北和江淮流域发生时间上同步、空间上尺度较大的极端干旱事件的概率较高,这也从侧面验证了旱涝指数序列中群发现象的存在;华北地区1260—1280年(对应的气候背景为中世纪暖期的末期)的旱涝指数对旱尺度因子的影响较1980—2000年(对应的气候背景为20世纪全球增暖)的情况更显著;1260—1280年这一时段旱或偏旱年数较1980—2000年也要多一些.因此,在气候较暖的时期可能易发生强度大、范围广的同步干旱事件,而近30年的中国北方干旱化可能是自然变率起主导作用下人为变率和自然变率共同作用的结果.

关键词：旱(涝)尺度因子持续性群发性指数分布

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斑鳢等鱼贝类去毒相关基因的克隆

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环境科学学报 2008年第11期28卷 2321-2326页

作者：林群梁旭方王琳胡永乐李光照暨南大学生命科学技术学院广州510632

采用RT-PCR和RACe法,克隆得到斑鳢、鲢鱼CYP1A与斑鳢HSP70基因cDNA的核心序列,序列长度分别为908bp、902bp、684bp,分别编码302、300、228个氨基酸;还克隆得到斑鳢、近江牡蛎GPX基因cDNA的部分序列,长度分别为720bp、727bp,分别编码119... 详细信息

采用RT-PCR和RACe法,克隆得到斑鳢、鲢鱼CYP1A与斑鳢HSP70基因cDNA的核心序列,序列长度分别为908bp、902bp、684bp,分别编码302、300、228个氨基酸;还克隆得到斑鳢、近江牡蛎GPX基因cDNA的部分序列,长度分别为720bp、727bp,分别编码119、232个氨基酸.多个重要养殖水生动物的去毒相关基因的成功克隆,为水产养殖动物去毒相关基因结构、功能、表达调控研究以及今后定向筛选高食用安全保障的水产品奠定基础.

关键词：去毒相关基因基因克隆水产养殖动物水产品安全

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in vitro degradation and biocompatibility of Mg-Nd-Zn-Zr alloy

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Chinese Science Bulletin 2012年第32期57卷 2163-2170页

作者： WANG YongPing He YaoHua ZHU ZhaoJin JiANG Yao ZHANG Jian NiU Jialin MAO lin YUAN GuangYin Department of Orthopaedics Sixth People's Hospital of Shanghai Jiao Tong UniversityShanghai 200233China National engineering Research Center of Light Alloys Net Forming School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China

in this study,in vitro degradation and biocompatibility of Mg-Nd-Zn-Zr(NZK) alloy were investigated to determine its suitability as a degradable medical *** corrosion properties were evaluated by static immersion test,electrochemical corrosion test,scanning electron microscopy(SeM),and energy dispersive spectroscopic(eDS) analysis,and in vitro biocompatibilities were assessed by hemolysis and cytotoxicity *** magnesium was used as *** results of static immersion test and electrochemical corrosion test in simulated body fluid(SBF) demonstrated that the addition of alloying elements could improve the corrosion *** hemolysis test found that the hemolysis rate of calcium phosphate coated NZK alloy was 4.8%,which was lower than the safe value of 5%.The cytotoxicity test indicated that NZK alloy extracts did not significantly reduce MC3T3-e1 cell *** test and cytotoxicity test display excellent hemocompatibility and cytocompatibility of NZK alloy in *** data indicate that NZK alloy has excellent biocompatibility and thus can be considered as a potential degradable medical biomaterial for orthopedic applications.

关键词：生物相容性体外降解锆合金纯镁电化学腐蚀试验医用生物材料扫描电子显微镜溶血试验

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Production of XX neo-male mandarin fish, Siniperca chuatsi, after sexual differentiation by oral administration of 17α-methyltestosterone

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Water Biology and Security 2023年第1期2卷 96-103页

作者： Jingjun Huang Shiyan Liu Haofeng Ouyang Chong Han Meihui Li linqiang Han Shuisheng Li Guifeng Li Haoran lin Yong Zhang State Key Laboratory of Biocontrol and School of Life Sciences Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic FishSun Yat-Sen UniversityGuangzhou510275PR China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao266373PR China Guangdong Liangshi Aquatic Seed industry Co. LtdFoshan528100PR China

A well-known fact about mandarin fish(Siniperca chuatsi)is that females grow faster than *** fish that have all-female populations have economic advantages,but traditional neo-male fish are difficult to *** this study,indirect feeding of 500 mg/kg 17α-methyltestosterone(MT)to XX mandarin fish(60 dpf,days post fertilization)with completely differentiated ovaries induced secondary sex *** 40 days after MT treatment,14.7%of mandarin fish with complete reversal was produced,which showed for the first time that XX mandarin fishes with differentiated ovaries still have potentially bisexual germ cells and the plasticity of sex *** the MT treatment group,there was no significant difference in survival compared to the control *** body weight of the MT treatment group was significantly lower than that of the control group 40 days after treatment(dat)(Ping MT treatment,serum 11-KT and T concentrations increased and serum e2 concentration decreased in the MT treatment group,but there was no significant difference in hormone levels between the MT treatment group and the control group.A masculinization of mandarin fish was also confirmed by the expression patterns of sex-specific genes sox9,dmrt1,cyp19a1a and foxl2.A significant amount of sox9 and dmrt1 was expressed in the fish gonads of the MT treatment group,whereas cyp19a1a and foxl2 were minimally expressed in the fish gonads.

关键词： 17α-methyltestosterone(MT) Mandarin fish Sex reversal Sex-specific genes

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