Genetic Evidence of Middle East Respiratory Syndrome Coronavirus(MERS-Cov) and Widespread Seroprevalence among Camels in Kenya

作     者：Sheila Ommeh Wei Zhang Ali Zohaib Jing Chen Huajun Zhang Ben Hu Xing-Yi Ge Xing-Lou Yang Moses Masika Vincent Obanda Yun Luo Shan Li Cecilia Waruhiu Bei Li Yan Zhu Desterio Ouma Vincent Odendo Lin-Fa Wang Danielle E.Anderson Jacqueline Lichoti Erick Mungube Francis Gakuya Peng Zhou Kisa-Juma Ngeiywa Bing Yan Bernard Agwanda Zheng-Li Shi 

作者机构：Institute for Biotechnology ResearchJomo Kenyatta University of Agriculture and TechnologyNairobi 62000-00200Kenya CAS Key Laboratory for Special Pathogens and BiosafetyWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China College of BiologyHunan University410006China Department of Medical MicrobiologyUniversity of NairobiNairobi 30197-00100Kenya Veterinary Services DepartmentKenya Wildlife ServiceNairobi 40241-00100Kenya Veterinary Research InstituteKenya Agriculture and Livestock Research OrganizationNairobi 57811-00200Kenya Veterinary Services Programme in Emerging Infectious DiseasesDuke-NUS Medical SchoolSingapore 169857Singapore Directorate of Veterinary ServicesState Department of LivestockMinistry of AgricultureLivestock Fisheries and IrrigationNairobi 34188-00100Kenya Kenya Camel AssociationNairobi 30095-00100Kenya Department of ZoologyNational Museums of KenyaNairobi 40658-00100Kenya 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2018年第33卷第6期

页      面：484-492页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：funded by Sino-Africa Joint Research Center (SAJC201313 and SAJC201605) External Cooperation Program of CAS (153211KYSB20160001) National Science and Technology Major Project (2018ZX0101004) 

主　　题：Middle East respiratory syndrome coronavirus(MERS-CoV) One-health Public health Zoonosis Kenya 

摘      要：We describe the first genome isolation of Middle East respiratory syndrome coronavirus(MERS-CoV) in Kenya. This fatal zoonotic pathogen was first described in the Kingdom of Saudi Arabia in 2012. Epidemiological and molecular evidence revealed zoonotic transmission from camels to humans and between humans. Currently, MERS-CoV is classified by the WHO as having high pandemic potential requiring greater surveillance. Previous studies of MERS-CoV in Kenya mainly focused on site-specific and archived camel and human serum samples for antibodies. We conducted active nationwide cross-sectional surveillance of camels and humans in Kenya, targeting both nasal swabs and plasma samples from 1,163 camels and 486 humans collected from January 2016 to June 2018. A total of 792 camel plasma samples were positive by ELISA. Seroprevalence increased with age, and the highest prevalence was observed in adult camels(82.37%, 95%confidence interval(CI) 79.50–84.91). More female camels were significantly seropositive(74.28%, 95% CI 71.14–77.19)than male camels(P \ 0.001)(53.74%, 95% CI 48.48–58.90). Only 11 camel nasal swabs were positive for MERS-CoV by reverse transcription-quantitative PCR. Phylogenetic analysis of whole genome sequences showed that Kenyan MERSCoV clustered within sub-clade C2, which is associated with the African clade, but did not contain signature deletions of orf4 b in African viruses. None of the human plasma screened contained neutralizing antibodies against MERS-CoV. This study confirms the geographically widespread occurrence of MERS-CoV in Kenyan camels. Further one-health surveillance approaches in camels, wildlife, and human populations are needed.